Immune Response of the Duck to Particulate (Red Blood Cell) Antigens

1981 ◽  
Vol 25 (2) ◽  
pp. 353 ◽  
Author(s):  
T. E. Toth ◽  
N. E. Norcross
Keyword(s):  
Immune Response ◽  
Blood Cell ◽  
Red Blood Cell

scholarly journals Expression and characterization of recombinant anti-Rh(D) antibodies on filamentous phage: a model system for isolating human red blood cell antibodies by repertoire cloning

Blood ◽  
1994 ◽  
Vol 83 (8) ◽  
pp. 2334-2344 ◽  
Author(s):  
DL Siegel ◽  
LE Silberstein
Keyword(s):  
Immune Response ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Expression Vectors ◽  
Filamentous Phage ◽  
Soluble Antigen ◽  
Fab Fragments ◽  
Human Immune Response ◽  
Membrane Bound ◽  
Human Immune

Abstract The production of human anti-red blood cell (RBC) Igs in vitro from immunized individuals would greatly facilitate the genetic analysis of the human immune response to RBC antigens and also provide useful serologic reagents. Technical difficulties inherent in human B-cell immortalization have led to the development of molecular approaches that bypass the need for cell transformation. By cloning human Ig gene segments into bacterial expression vectors, libraries are created of filamentous phage particles displaying Fab fragments on their surfaces. Libraries have been screened with purified, soluble antigen and selected clones genetically manipulated in Escherichia coli to produce soluble Fab fragments. Our goal has been to adapt this technique to the study of RBC autoantibodies and alloantibodies that have specificities against unpurifiable membrane-bound antigens. To test the feasibility of this approach, two sets of phage were created, one set expressing a human anti-Rh(D) Ig and the other expressing a human antitetanus toxoid Ig. After verifying the presence of functional phage-displayed Fabs through biochemical, flow cytometric, and electron microscopic analyses, a model library was constructed comprising one anti-Rh(D)- expressing phage per 10(4) antitetanus toxoid-expressing phage. A method was developed for screening the library with intact Rh(D)- positive RBCs. After four rounds of panning, anti-Rh(D) specificity was enriched more than 10,000-fold to a final frequency of approximately 100%. Plasmid DNA derived from anti-Rh(D) phage was used to produce milligram quantities of soluble recombinant anti-Rh(D) Fabs purified by nitrogen cavitation and nickel-chelation affinity chromatography. The authenticity of the Fabs was confirmed by sodium dodecyl sulfate- polyacrylamide gel electrophoresis and immunoblotting, which showed bands with molecular weights of approximately 50 kD and 26 kD under nonreducing and reducing conditions, respectively. Binding of recombinant anti-Rh(D) Fabs to Rh(D)-positive RBCs was demonstrated by flow cytometry and by an agglutination assay. Our results suggest that repertoire cloning by cell-surface enrichment may have broad application to the study of the human immune response to erythroid antigens in addition to membrane-bound antigens present on other hematopoietic cells.

scholarly journals Red Blood Cell Released ATP Regulates Systemic Immune Response in Atherosclerosis

2021 ◽  
Vol 35 (S1) ◽  
Author(s):  
Haoyu Sun ◽  
Yunpei Zhang ◽  
Yong Du ◽  
Pingnian He
Keyword(s):  
Immune Response ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Systemic Immune Response

Immune Response to Chronic Red Blood Cell Transfusion

Vox Sanguinis ◽  
1983 ◽  
Vol 44 (4) ◽  
pp. 212-217 ◽  
Author(s):  
Neil Blumberg ◽  
Kathy Peck ◽  
Karen Ross ◽  
Eduardo Avila
Keyword(s):  
Immune Response ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Red Blood Cell Transfusion

Hawthorn (Crataegus monogyna) Phenolic Extract Modulates Lymphocyte Subsets and Humoral Immune Response in Mice

Planta Medica ◽  
2019 ◽  
Vol 86 (02) ◽  
pp. 160-168 ◽  
Author(s):  
Magdalena Lis ◽  
Marianna Szczypka ◽  
Agnieszka Suszko-Pawłowska ◽  
Anna Sokół-Łętowska ◽  
Alicja Kucharska ◽  
...  
Keyword(s):  
Immune Response ◽  
T Lymphocytes ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Humoral Immune Response ◽  
Lymphocyte Subsets ◽  
Mesenteric Lymph Nodes ◽  
Crataegus Monogyna ◽  
Humoral Immune ◽  
Phenolic Extract

AbstractThis study investigated the effect of hawthorn (Crataegus monogyna) phenolic extract on lymphocyte subsets in the lymphoid organs in nonimmunized mice and on humoral immune response in sheep red blood cell-immunized mice. Hawthorn phenolic extract (50, 100, 200 mg/kg) was administered orally five or ten times. Sheep red blood cells were injected 24 h after administration of the last extract dose. The lymphocyte subsets were assessed 24 and 72 h after the last dose. Humoral immune response was determined 4 and 7 days after immunization. Five doses of the extract decreased the percentage of CD4−CD8− and CD4+ thymocytes but elevated the percentage of CD4+CD8+ and CD8+ thymic cells. The extract increased the total number, percentage, and absolute count of T and B splenocytes. When administered five times, it lowered the percentage of T lymphocytes, but boosted the population of B lymphocytes of mesenteric lymph nodes (after 24 h). However, a rise in the population of T lymphocytes was observed 72 h after five and ten doses. The extract administered ten times elevated the number of plaque-forming cells and total anti-sheep red blood cell hemagglutinin titer but reduced the 2-ME-resistant antibody titer (day 7). At the same time, five doses of the extract increased antibody titers. Considering its impact on lymphocyte subsets and humoral immune response, hawthorn extract may be used as an immunomodulator.

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