scholarly journals Characterization of the Pattern of Inflammatory Cell Influx in Chicks Following the Intraperitoneal Administration of Live Salmonella enteritidis and Salmonella enteritidis-Immune Lymphokines

1995 ◽  
Vol 74 (1) ◽  
pp. 8-17 ◽  
Author(s):  
MICHAEL H. KOGUT ◽  
EDWARD D. MCGRUDER ◽  
BILLY M. HARGIS ◽  
DONALD E. CORRIER ◽  
JOHN R. DELOACH
Keyword(s):  
Salmonella Enteritidis ◽  
Inflammatory Cell ◽  
Intraperitoneal Administration ◽  
Immune Lymphokines

Activation of isolated heterophils from chicks stimulated in vivo with salmonella enteritidis-immune lymphokines

1996 ◽  
Vol 54 ◽  
pp. 760-761
Author(s):  
R. B. Moyes ◽  
R. E. Droleskey ◽  
M. H. Kogut ◽  
J. R. DeLoach
Keyword(s):  
Lamina Propria ◽  
Intestinal Mucosa ◽  
Salmonella Enteritidis ◽  
Intestinal Tract ◽  
Polymorphonuclear Cells ◽  
Subclinical Infection ◽  
Egg Products ◽  
Immune Lymphokines ◽  
Organ Invasion

Salmonella enteritidis (SE) is of great concern to the poultry industry due to the organism's ability to penetrate the intestinal mucosa of the laying hen and subsequently colonize the ovaries and yolk membrane. The resultant subclinical infection can lead to SE infection of raw eggs and egg products. Interference with the ability of the organism to invade has been linked to the activation and recruitment of inflammatory polymorphonuclear cells, heterophils, to the lamina propria of the intestinal tract.Recently it has been established that heterophil activation and increased resistance to SE organ invasion can be accomplished by the administration of SE-immune lymphokines (SE-ILK) obtained from supernatants of concanavalin-A stimulated SE immune T lymphocytes from SE hyperimmunized hens. Invasion of SE into the lamina propria provides a secondary signal for directing activated heterophils to the site of SE invasion.

Presence of Interferon-Gamma and IL-2 in Supernatants of Salmonella enteritidis-Immune Lymphokines

2009 ◽  
Vol 8 (9) ◽  
pp. 820-823
Author(s):  
Gabriela V. G- Verduzco ◽  
Guillermo Tellez ◽  
Michael H. Kogut ◽  
Armando Isibasi ◽  
Vianney Ortiz- Navarrete
Keyword(s):  
Interferon Gamma ◽  
Salmonella Enteritidis ◽  
Immune Lymphokines

scholarly journals Lactate Production Precedes Inflammatory Cell Recruitment in Arthritic Ankles: an Imaging Study

2020 ◽  
Vol 22 (5) ◽  
pp. 1324-1332
Author(s):  
Marie-Aline Neveu ◽  
Nicolas Beziere ◽  
Rolf Daniels ◽  
Caroline Bouzin ◽  
Arnaud Comment ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Inflammatory Cell ◽  
Immune Cell ◽  
Lactate Production ◽  
High Rate ◽  
Metabolic Shift ◽  
Pyruvate Metabolism ◽  
Cell Recruitment ◽  
Inflammatory Cell Recruitment

Abstract Purpose Inflammation is involved in many disease processes. However, accurate imaging tools permitting diagnosis and characterization of inflammation are still missing. As inflamed tissues exhibit a high rate of glycolysis, pyruvate metabolism may offer a unique approach to follow the inflammatory response and disease progression. Therefore, the aim of the study was to follow metabolic changes and recruitment of inflammatory cells after onset of inflammation in arthritic ankles using hyperpolarized 1-13C-pyruvate magnetic resonance spectroscopy (MRS) and 19F magnetic resonance imaging (MRI), respectively. Procedure Experimental rheumatoid arthritis (RA) was induced by intraperitoneal injection of glucose-6-phosphate-isomerase-specific antibodies (GPI) containing serum. To monitor pyruvate metabolism, the transformation of hyperpolarized 1-13C-pyruvate into hyperpolarized 1-13C-lactate was followed using MRS. To track phagocytic immune cell homing, we intravenously injected a perfluorocarbon emulsion 48 h before imaging. The animals were scanned at days 1, 3, or 6 after GPI-serum injection to examine the different stages of arthritic inflammation. Finally, to confirm the pyruvate metabolic activity and the link to inflammatory cell recruitment, we conducted hematoxylin-eosin histopathology and monocarboxylase transporter (MCT-1) immune histochemistry (IHC) of inflamed ankles. Results Hyperpolarized 1-13C-pyruvate MRS revealed a high rate of lactate production immediately at day 1 after GPI-serum transfer, which remained elevated during the progression of the disease, while 19F-MRI exhibited a gradual recruitment of phagocytic immune cells in arthritic ankles, which correlated well with the course of ankle swelling. Histopathology and IHC revealed that MCT-1 was expressed in regions with inflammatory cell recruitment, confirming the metabolic shift identified in arthritic ankles. Conclusions Our study demonstrated the presence of a very early metabolic shift in arthritic joints independent of phagocytic immune cell recruitment. Thus, hyperpolarized 1-13C-pyruvate represents a promising tracer to monitor acute arthritic joint inflammation, even with minor ankle swelling. Furthermore, translated to the clinics, these methods add a detailed characterization of disease status and could substantially support patient stratification and therapy monitoring.

scholarly journals Purification and characterization of thin, aggregative fimbriae from Salmonella enteritidis.

1991 ◽  
Vol 173 (15) ◽  
pp. 4773-4781 ◽  
Author(s):  
S K Collinson ◽  
L Emödy ◽  
K H Müller ◽  
T J Trust ◽  
W W Kay
Keyword(s):  
Salmonella Enteritidis ◽  
Purification And Characterization

Immunohistochemical characterization of seminoma and its inflammatory cell infiltrate

1987 ◽  
Vol 18 (5) ◽  
pp. 511-520 ◽  
Author(s):  
Debra A. Bell ◽  
Thomas J. Flotie ◽  
Atul K. Bhan
Keyword(s):  
Inflammatory Cell ◽  
Inflammatory Cell Infiltrate ◽  
Cell Infiltrate

scholarly journals Dietary Selenium for the counteraction of oxidative damage: fortified foods or supplements?

2007 ◽  
Vol 99 (1) ◽  
pp. 191-197 ◽  
Author(s):  
Alessandra Bordoni ◽  
Francesca Danesi ◽  
Marco Malaguti ◽  
Mattia Di Nunzio ◽  
Francesca Pasqui ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Intraperitoneal Administration ◽  
Dietary Advice ◽  
Glutathione Peroxidase Activity ◽  
Significant Modification ◽  
Fortified Foods ◽  
Male Wistar Rats ◽  
Different Sources ◽  
Se Status

Since any significant modification in the Se status, leading to changes in the activity of the seleno-enzymes, may have important consequences on the susceptibility of tissues to oxidative stress, considerable efforts have been made upon increasing Se dietary intake. In this respect, an important debate is still open about the bioavailability and the effectiveness of Se, and more generally nutrients, in supplements compared with foods. Using male Wistar rats, we have compared the effectiveness of two different diets in which an adequate Se content (0·1 mg/kg) was achieved by adding the element as sodium selenite or as component of a lyophilized Se-enriched food, in the counteraction of an oxidative stress induced by intraperitoneal administration of adriamycin. Both Se-enriched diets were able to reduce the consequences of the oxidative stress in liver, mainly by increasing glutathione peroxidase activity. This increase was more evident in rats fed on the diet enriched with the lyophilized food, probably due to the different chemical forms of Se, or to other components of the food itself. Although further studies are needed, data herein presented may contribute to the characterization of the effectiveness of Se from different sources, foods or supplements, in the light of dietary advice to the population concerning improvement of Se intake.

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