scholarly journals Xylose-Lysine-Tergitol 4: An Improved Selective Agar Medium for the Isolation of Salmonella

1991 ◽  
Vol 70 (12) ◽  
pp. 2429-2432 ◽  
Author(s):  
RUSSELL G. MILLER ◽  
C.R. TATE ◽  
E.T. MALLINSON ◽  
J.A. SCHERRER
Keyword(s):  
Agar Medium ◽  
Selective Agar

Assessment of different selective agar media for enumeration and isolation ofListeriafrom dairy products

1988 ◽  
Vol 55 (4) ◽  
pp. 579-583 ◽  
Author(s):  
Lucas Dominguez ◽  
José Francisco Fernández ◽  
Victor Briones ◽  
José Luis Blanco ◽  
Guillermo Suárez
Keyword(s):  
Dairy Products ◽  
Agar Medium ◽  
Raw Milk ◽  
Superior Performance ◽  
Direct Plating ◽  
Selective Agar ◽  
Natural Microflora ◽  
Agar Media ◽  
The Difference ◽  
Better Than

SummaryDifferent selective agar media were compared for the recovery and isolation of five species ofListeriafrom raw milk and cheese. The selective media examined were Beerens medium, MacBride medium and that described by Dominguezet al.(1984) with 6 mg/1 acriflavine, listeria selective agar medium (LSAM), and LSAM with 12 mg/1 acriflavine (LSAM × 2A); a non-selective yeast glucose Lemco agar was included for comparison. When the difference between listeria and the natural microflora of raw milk and cheese was 102cfu/ml, listeria could be isolated by direct plating on all media tested. When it was lower than 103–104cfu/ml, listeria were isolated by direct plating only on LSAM and LSAM × 2A. When the difference was greater than 104cfu/ml, a previous enrichment was necessary to isolate them. LSAM and LSAM × 2A media performed better than the other media tested for isolating listeria by direct plating and improved their isolation from dairy products. This superior performance was evaluated by the ability of these media to support colony formation of different species ofListeriatested, the easy recognition of these colonies from those formed by other microorganisms and by their capacity to inhibit the natural microflora of these foods.

Procedures for Recovery of Stressed and Injured Cells of Yersinia Enterocolitica from Meat and Meat Products

1989 ◽  
Vol 52 (5) ◽  
pp. 360-362 ◽  
Author(s):  
ZHEKO KOUNEV
Keyword(s):  
Yersinia Enterocolitica ◽  
Agar Medium ◽  
Alkali Treatment ◽  
Meat Products ◽  
The Other ◽  
Step Procedure ◽  
Selective Agar ◽  
Agar Media ◽  
Phosphate Buffered Saline

A new three-step procedure (TSP) for the recovery of Yersinia enterocolitica 0:3 from frozen meat, salted and dried meat products, raw dried meat products, and cooked perishable sausages, has been developed. The TSP is based mainly on enrichment in 0.15 M phosphate-buffered saline at 25°C. In the TSP, selected dilutions of samples are enriched for 1, 2, 3, 4, 24, and 48 h and then plated onto nonselective and selective agar media after or without alkali treatment. Additional enrichment was performed with half of the samples at 25°C for 24 h, and the rest at 4°C for up to 2 wk, followed by alkali treatment and plating on selective agar medium. Recovery of Y. enterocolitica was better using the TSP than the other method used for isolating the organism from meats.

Screening of Rhizobium leguminosarum bv. trifolii for adaptation to acid and neutral soils using a selective agar medium

1993 ◽  
Vol 25 (10) ◽  
pp. 1463-1464 ◽  
Author(s):  
L.G. Gemell ◽  
R.J. Roughley ◽  
M.L. Reed ◽  
Elizabeth J. Hartley
Keyword(s):  
Rhizobium Leguminosarum ◽  
Agar Medium ◽  
Selective Agar

scholarly journals Antimicrobial resistance of bacterial isolates from dierent dairy products and their emergence in the food chain

2018 ◽  
Vol 11 (1) ◽  
pp. 45-57
Author(s):  
É. György ◽  
É. Laslo
Keyword(s):  
Risk Factors ◽  
Antibiotic Resistance ◽  
Foodborne Pathogens ◽  
Dairy Products ◽  
Preventive Measures ◽  
Agar Medium ◽  
Bacterial Isolates ◽  
E Coli ◽  
Selective Agar ◽  
Overall Resistance

Abstract The antibiotic resistance of foodborne pathogens represents a healthcare concern globally. This phenomenon has an increasing impact on medicine and economy. A total of 26 spoilage and pathogenic bacterial isolates originating from different dairy products have been screened against eight different antibiotics. Based on the type of the selective agar medium used for their isolation, the isolates were: five staphylococci isolates, six Vibrio isolates, two Pseudomonas sp. isolates, three Salmonella isolates, five E. coli isolates, and five coliform isolates. The overall resistance to the tested antimicrobials of the bacterial isolates was 31.73%, the majority being susceptible. Based on the results, there are isolates with multiple antibiotic patterns that can be possible risk factors and may call for preventive measures.

scholarly journals STUDY ON PATHOGENICITY OF PASTEURELLA MULTOCIDA WHICH ISOLATED FROM FARM ANIMALS AND MAN

2021 ◽  
Vol 26 (1) ◽  
pp. 74-81
Author(s):  
S. S. A. Mobarak ◽  
A. K. Shubber ◽  
A. S. Raheem
Keyword(s):  
Pasteurella Multocida ◽  
Culture Media ◽  
Agar Medium ◽  
Farm Animals ◽  
Pathogenicity Test ◽  
Infected Farm ◽  
Human Samples ◽  
Selective Agar ◽  
Suppurative Pneumonia ◽  
Animal Isolates

This study was described for the nature of the pathpgenesis of bacteria Pasteurella multocida which was isolated from infected man made comparison between these bacteria and those from infected farm animals. The percentage of Pasteurella multcida diagnosed bacteria from animals and human was 29.4% and 16.9% respectively. Comparing to other culture media Pasteurella multocida selective agar medium was characterized by its selectivity and sensitivity and then was attempt for biotyping species and subspecies of isolated Pasteurella from animals and human samples were successfully achieved. Pathogenicity test was performed on mice, only nine human isolatetes and twenty-one animal isolates from Pasteurella multocida were virulent. Todistinguish between the pathogenesis of human and animal isolates, one isolated from human and animal were chosed, in addition to the standared strain. The mice had been experimentally infected by three different ways, I/P, I/T, I/Eye. The results were showed that Pasteurella multocida can produce lesions as fibrinous suppurative pneumonia in lungs, liver and spleen which were detected histopatho logically. However the animal isolates were more virulent than human or standared strain.

Seed-to-seedling transmission of Botrytis cinerea in chickpea and disinfestation of seed with moist heat

1997 ◽  
Vol 37 (2) ◽  
pp. 223 ◽  
Author(s):  
D. R. Burgess ◽  
T. Bretag ◽  
P. J. Keane
Keyword(s):  
Heat Treatment ◽  
Adverse Effect ◽  
Botrytis Cinerea ◽  
Seedling Establishment ◽  
Agar Medium ◽  
Soft Rot ◽  
Isolation Frequency ◽  
Eastern Australia ◽  
Selective Agar ◽  
Stem Lesions

Summary. Chickpea crops in south-eastern Australia are affected in some seasons by poor emergence and seedling soft rot. Botrytis cinerea was isolated on a semi-selective agar medium from diseased seedlings and from samples of seed submitted by growers. The frequency of isolation from seed harvested in 1993–94 ranged from 0 to 95%. Higher isolation rates were associated with reductions in seedling establishment in 1994 estimated at 30–75%. Surface sterilisation studies showed that infection was largely external on the seed. In growth cabinet experiments at 8–18°C, seedlings grown from infected seed or from seed inoculated with B. cinerea developed soft rot, and sporulation of the pathogen on the basal stem lesions was observed. Treatment of moist seed at 50°C for 5 min or storage of seed at 20°C for 12 months reduced the frequency of isolation of B. cinerea to 0 and 2%, respectively, with no adverse effect on germination. Heat treatment of freshly harvested seed reduced the isolation frequency from 98 to 18%. The applicability of heat treatment for seed disinfestation by chickpea growers is discussed.

Evaluation of a new selective agar medium for detection of carbapenem-resistant Enterobacteriaceae

2019 ◽  
Vol 95 (4) ◽  
pp. 114882
Author(s):  
Kyoko Kuwahara-Arai ◽  
Ayaka Morito ◽  
Izumi Yanagisawa ◽  
Tomomi Hishinuma ◽  
San Mya ◽  
...  
Keyword(s):  
Agar Medium ◽  
Carbapenem Resistant ◽  
Selective Agar ◽  
Carbapenem Resistant Enterobacteriaceae
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