scholarly journals Genetic and Phenotypic Correlations between Immune Response to Escherichia coli and to Newcastle Disease Virus Vaccines

1981 ◽  
Vol 60 (1) ◽  
pp. 49-53 ◽  
Author(s):  
M. SOLLER ◽  
D. HELLER ◽  
B. PELEG ◽  
NILI RON-KUPER ◽  
K. HORNSTEIN
Keyword(s):  
Escherichia Coli ◽  
Immune Response ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Phenotypic Correlations ◽  
Genetic And Phenotypic Correlations

scholarly journals Optimization of Human Immunodeficiency Virus Gag Expression by Newcastle Disease Virus Vectors for the Induction of Potent Immune Responses

2008 ◽  
Vol 83 (2) ◽  
pp. 584-597 ◽  
Author(s):  
Elena Carnero ◽  
Wenjing Li ◽  
Antonio V. Borderia ◽  
Bruno Moltedo ◽  
Thomas Moran ◽  
...  
Keyword(s):  
Immune Response ◽  
Human Immunodeficiency Virus ◽  
Immune Responses ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Insertion Site ◽  
Gag Protein ◽  
Immunodeficiency Virus

ABSTRACT One attractive strategy for the development of a human immunodeficiency virus (HIV) vaccine is the use of viral vectors with a proven safety profile and an absence of preexisting immunity in humans, such as Newcastle disease virus (NDV). Several NDV vaccine vectors have been generated, and their immunogenicities have been investigated with different animal models. However, a systematic study to evaluate the optimal insertion site of the foreign antigens into NDV that results in enhanced immune responses specific to the antigen has not yet been conducted. In this article, we describe the ability of NDV expressing HIV Gag to generate a Gag-specific immune response in mice. We also have determined the optimal insertion site into the NDV genome by generating recombinant NDV-HIVGag viruses in which HIV gag was located at different transcriptional positions throughout the NDV viral genome. All recombinant viruses were viable, grew to similar titers in embryonated chicken eggs, and expressed Gag in a stable manner. Our in vivo experiments revealed that higher HIV Gag protein expression positively correlates with an enhanced CD8+ T-cell-mediated immune response and protective immunity against challenge with vaccinia virus expressing HIV Gag. We also inserted a codon-optimized version of HIV gag in the described best location, between the P and M genes. Virus expressing the codon-optimized version of HIV gag induced a higher expression of the protein and an enhanced immune response against HIV Gag in mice. These results indicate that strategies directed toward increasing antigen expression by NDV result in enhanced immunogenicity and vaccine efficacy.

INTRANASAL DELIVERY OF MANNAN-COATED LIPOSOMES ENHANCES ANTIBODY RESPONSES TO A NEWCASTLE DISEASE VIRUS VACCINE

2010 ◽  
Vol 22 (01) ◽  
pp. 1-7 ◽  
Author(s):  
Li-Ping Tseng ◽  
Chwei-Jang Chiou ◽  
Ming-Chung Deng ◽  
Yi-You Huang ◽  
Der-Zen Liu
Keyword(s):  
Immune Response ◽  
Survival Rate ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Immunoglobulin A ◽  
Adaptive Immune Response ◽  
Mucosal Vaccination ◽  
Adaptive Immune ◽  
Newcastle Disease Virus Vaccine

The polysaccharide, mannan, has been shown to be an effective immunoprotective agent and vaccine adjuvant in a variety of bacterial and viral diseases in different animal species. The objective of this study was to evaluate the immune response of chickens after being intranasal (i.n.) immunized using the Newcastle disease virus (NDV) encapsulated in liposomes and coated with mannan. Groups of chickens were vaccinated once intranasally with virus alone, then with uncoated liposome-encapsulated NDV (NC-lip), or mannan-coated liposome-encapsulated NDV (Man-lip). On day 14, velogenic viral challenge was carried out on vaccinated chickens. Chickens receiving virus alone had a 20% survival rate. In contrast, groups that received Man-lip had a higher survival rate of 70% (P < 0.01). The titers of mucosal secretary immunoglobulin A ( s-IgA ) was the highest in the group for the birds that received Man-lip as mucosal adjuvant (P < 0.01). These results suggest that liposomes coated with mannan may thus be used as a potential adjuvant for mucosal vaccination against NDV. This is the first study in which mannan has been demonstrated to enhance an adaptive immune response against ND virus infection in chickens.

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