scholarly journals In Vitro Uptake of Fe59 in Adult and Immature Avian and Human Red Cells

1963 ◽  
Vol 42 (3) ◽  
pp. 595-598
Author(s):  
R.P. Reckel ◽  
S.L. Scheinberg
Keyword(s):  
Red Cells ◽  
In Vitro Uptake ◽  
Human Red Cells

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

scholarly journals Some Interrelationships of Blood and the Fava Bean Principle in Vitro

Blood ◽  
1952 ◽  
Vol 7 (7) ◽  
pp. 721-728 ◽  
Author(s):  
WILLIAM P. CREGER ◽  
HOUGHTON GIFFORD
Keyword(s):  
Animal Species ◽  
Gamma Globulin ◽  
Test Tube ◽  
Red Cells ◽  
Red Cell ◽  
Fava Bean ◽  
Increased Susceptibility ◽  
Human Red Cells

Abstract 1. Saline suspensions of human red cells, as well as those of several animal species, were agglutinated by normal saline extracts of the Fava bean. 2. This agglutination was potentiated in titer 100-fold in a medium of 10 per cent acacia, as a diluent. 3. The inhibition of the hemagglutination action of the Fava bean extract by human serum was apparently attributable to the gamma globulin fraction. 4. The Fava bean principle could be transferred from cell to cell, as shown by heat-elution and acacia technics. 5. Fava-sensitized red cells did not exhibit increased susceptibility in the test tube to complement, hemolysin, or osmotic or mechanical fragility. 6. The mechanism of in vivo red cell destruction in Favism is as yet unknown, but a special immunologic susceptibility to the action of the bean’s principle is suspected in certain persons. 7. It is suggested that the relation of acacia to Fava-sensitized red cells may form the basis of a diagnostic test for Favism in the early, acute stages of the disease.

Influence of Cell Age and Ketoaminic Linkage on Rapid Glycosylation of Hemoglobin in Human Red Cells In Vitro

1985 ◽  
Vol 17 (04) ◽  
pp. 201-204 ◽  
Author(s):  
R. Cordera ◽  
G. Andraghetti ◽  
R. Bonadonna ◽  
R. Gherzi ◽  
M. Maiello ◽  
...  
Keyword(s):  
Red Cells ◽  
Cell Age ◽  
Human Red Cells

scholarly journals Hemoglobin Charge Dependence on Hemoglobin Concentration in Vitro

1971 ◽  
Vol 57 (3) ◽  
pp. 283-289 ◽  
Author(s):  
C. M. Gary-Bobo ◽  
A. K. Solomon
Keyword(s):  
External Solution ◽  
Hemoglobin Concentration ◽  
Bulk Water ◽  
Red Cells ◽  
A Value ◽  
Charge Dependence ◽  
Hemoglobin Molecule ◽  
Sigmoid Curve ◽  
Human Red Cells

Studies have been made of the dependence of the charge of the hemoglobin molecule on hemoglobin concentration in the concentration range between 3 and 11 mmolal. The charge has been determined by measuring the distribution of 42K between a hemoglobin solution in a cellophane bag and an external solution. The pH was 6.6, the K concentration was 10 mM, and the temperature was 4°C. The charge decreased along a sigmoid curve from a value of +3 in the most dilute solutions to a value of +0.5 in the most concentrated solutions. The results were in excellent agreement with earlier studies of Gary-Bobo and Solomon in which Cl distribution was measured between human red cells and external solutions and thus give added support to the conclusion that the apparent anomalous osmotic behavior of human red cells may be attributed to concentration-dependent changes in the hemoglobin net charge. The present findings also support the view that the water in the red cell is solvent water for K and Cl and differs in no quantitatively important respect from bulk water in free solution.

Calmodulin carboxylmethyl ester formation in intact human red cells and modulation of this reaction by divalent cations in vitro

FEBS Letters ◽  
1982 ◽  
Vol 147 (1) ◽  
pp. 125-130 ◽  
Author(s):  
L. Runte ◽  
H.-L. Jürgensmeier ◽  
C. Unger ◽  
H.D. Söling
Keyword(s):  
Divalent Cations ◽  
Red Cells ◽  
Ester Formation ◽  
Human Red Cells

IN VITRO UPTAKE OF Fe59 BY BLOOD CELLS FROM NORMAL BIRDS AND BIRDS WITH ERYTHROBLASTOSIS

1963 ◽  
Vol 41 (1) ◽  
pp. 2057-2064 ◽  
Author(s):  
R. Bather ◽  
Stella Dzuibalo ◽  
C. le Q. Darcel
Keyword(s):  
Bone Marrow ◽  
Ammonium Sulphate ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Blood ◽  
Promoting Effect ◽  
Heme Synthesis ◽  
Heat Resistant ◽  
In Vitro Uptake

The cellular elements of blood obtained from birds with virus-induced erythroblastosis rapidly incorporated radioactivity when incubated in vitro with Fe59Cl3. This effect was not seen until primitive red cells entered the circulating blood. Little of the iron that was incorporated reached heme. Instead most of it was retained by a heat-resistant component which was precipitable with 35% ammonium sulphate and had properties suggestive of a ferritin-like substance. Plasma from leukemic blood had a slight promoting effect on incorporation of Fe59 into normal blood cells in vitro when compared with normal plasma.Blood cells from birds in which the bone marrow was rendered hyperactive with phenylhydrazine also incorporated Fe59 in vitro, a large proportion of this reaching heme. Incorporation into the heme fraction was also observed in blood cells from erythroblastosis-infected birds treated with phenylhydrazine, indicating that heme synthesis was not completely blocked in these birds.

scholarly journals Neutrophils mediate lipid peroxidation in human red cells

Blood ◽  
1984 ◽  
Vol 64 (5) ◽  
pp. 1079-1084 ◽  
Author(s):  
S Claster ◽  
DT Chiu ◽  
A Quintanilha ◽  
B Lubin
Keyword(s):  
Lipid Peroxidation ◽  
Red Cells ◽  
Red Cell ◽  
Normal Cells ◽  
Sickle Cells ◽  
Induce Lipid Peroxidation ◽  
Activated Neutrophils ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Human Red Cells

Abstract Activated neutrophils (ANs) are known to release reactive oxygen species that may cause oxidative damage to surrounding tissues. We determined if ANs could induce lipid peroxidation (LP) in human red cells and investigated the mechanism involved in this interaction. We studied neonatal glucose-6-phosphate dehydrogenase (G6PD) deficient, and sickle red cells, since each of these are known to be susceptible to oxidant injury. Neutrophils were isolated from whole blood and activated by incubation with opsonized zymosan. Mixtures of such neutrophils and red cells at a ratio of 1:100 were incubated for two hours at 37 degrees C, after which the malonyldialdehyde content in red cells was measured as an index of LP. All red cells underwent LP after AN treatment, and the degree of LP was proportional to the amount of AN in the mixture. Superoxide dismutase and catalase partially inhibited LP. When compared to normal red cells, only sickle cells demonstrated a significant increase in AN-mediated LP. Conversion of hemoglobin to carboxy-hemoglobin increased AN-mediated LP, whereas conversion to met- hemoglobin decreased AN-mediated LP. The protective effect of met- hemoglobin on LP was less in sickle cells than in normal cells. We conclude that AN can induce LP in red cells in vitro and that sickle cells are more susceptible to this process than normal cells. Hemoglobin can serve as an electron trap and protect the cell against peroxidative damage, but this mechanism is impaired in sickle cells. We speculate that the pathogenesis of hemolysis associated with infectious disease may include AN-induced red cell LP.

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