THE PHYTOCHEMICAL COMPOSITION AND THE EFFECT OF METHANOLIC EXTRACT OF TYPHA DOMINGENSIS PERS. FRUITE ON SOME BIOCHEMICAL PARAMETERS IN ADULT MALE RABBITS

2012 ◽  
Vol 11 (1) ◽  
pp. 224-228
Author(s):  
Arwa H.M.AL-Saeed
Keyword(s):  
Adult Male ◽  
Biochemical Parameters ◽  
Methanolic Extract ◽  
Typha Domingensis ◽  
Phytochemical Composition

Investigation of Oral Subacute Toxicity and In-Vitro Antioxidant Activity of Standardized Methanolic Extract of Quercus serrata Leaves

2020 ◽  
Vol 16 ◽  
Author(s):  
Maibam Beebina Chanu ◽  
Biseshwori Thongam ◽  
Khumukcham Nongalleima ◽  
Hans Raj Bhat ◽  
Surajit Kumar Ghosh ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Biochemical Parameters ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Quercus Serrata ◽  
Control Group ◽  
Blood Cell Count ◽  
Toxicological Profile

Background: Quercus serrata Murray leaves have been used traditionally in the treatment of diabetes, dysmenorrhoea, inflammation and urinary tract infection. So, far no study had been reported on the toxicological profile and antioxidant properties of the plant. Objective: The present study was aimed to investigate the in-vivo toxicological profile and in-vitro antioxidant activities of the methanolic extract of standardized Quercus serrata leaves. Methods: Per-oral sub-acute toxicity study was performed in rats using three dose levels (200, 400 and 800 mg/kg b.w.) of the extract for 28-days. Control group received gum acacia suspended in water. Bodyweight was measured weekly. Biochemical parameters were analysed using the serum, the blood-cell count was done using whole blood. Pathological changes were also checked in highly perfused tissues. Further, in-vitro reducing power assay, nitric oxide scavenging assay, DPPH free-radical scavenging assay were performed to check the antioxidant activity of the extract. Results: There were no significant alterations in the blood-cell count and biochemical parameters analysed in the treatment group when compared with the normal control. Histopathology study of liver, kidney, pancreas, heart and brain revealed normal cellular architecture in the treatment groups alike the control group animals. Quercus serrata also showed a significant reduction of DPPH with IC50 4.48±0.254 µg/mL, in-vitro reducing power activity with IC50121.65±0.320 µg/mL and nitric oxide scavenging activity IC50 106.43±0.338 µg/mL. Conclusion: The above study showed that standardized methanolic extract of Quercus serrata leaves was safe after subacute oral administration in rats and has good antioxidant potential.

scholarly journals Investigation on phytochemical composition, anti-oxidant and anticancer properties of methanolic extract of Phyllanthus niruri schumach & thonn. And protein modelling and drug docking

2020 ◽  
Vol 11 (4) ◽  
pp. 7937-7943
Author(s):  
Sugumar R ◽  
Saravana D ◽  
Pavithra N ◽  
Suresh Raj V ◽  
Sekar Babu M ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Docking Studies ◽  
Future Research ◽  
Total Phenolic ◽  
Human Beings ◽  
Protein Modelling ◽  
Phyllanthus Niruri ◽  
Anticancer Properties ◽  
Phytochemical Composition ◽  
Anti Oxidant

The study aims to investigate the Phytochemical Composition, Anti-oxidant and Anticancer properties of methanolic extract of Phyllanthus niruri Schumach & Thonn And the Protein Modelling and drug docking. The research deals with the methanolic extraction and phytochemical screening, determination of total phenolic and flavonoids contents and anti-oxidant assay. By performing GC-MS characterisation, various active metabolites are analysed. Thin-layer chromatography profiling of the Phyllanthus niruri methanolic extract was performed. The IC50 of the Phyllanthus niruri methanolic extract against PA-1 Cell lines(Ovarian cancer) was calculated. Docking studies also performed for antitumor activity by using Bioinformatics and Cheminformatics software on corilagin and cisplatin. The results suggested that the methanolic extract of Phyllanthus niruri leaves has the anticancer cancer effect on the ovarian cell line. The docking studies also performed that Corilagin interaction with T.F. receptor shows a high binding score when compared to cisplatin. Our future research can be done in this area to optimise anticancer activity efficacy. Our results can be further tested Clinico-pharmacologically to prove its efficiency in human beings.

scholarly journals Comparative Nephroprotective Effects of Extracts of Buchholzia coriacea on some Biochemical Parameters in Carbon Tetrachloride-induced Toxicity in Rattus novergicus

2018 ◽  
Vol 17 (2) ◽  
pp. 227-235
Author(s):  
Godwin C. Ugwu ◽  
Chinagorom L. Okanya ◽  
Jude V. Egbuji ◽  
Jude I. Okwo ◽  
Emmanuel I. Nnamonu ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Uric Acid ◽  
Carbon Tetrachloride ◽  
Medicinal Plant ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Methanolic Extract ◽  
Rat Kidney ◽  
Aqueous Extracts ◽  
Methanolic Extracts

Buchholzia coriacea (wonderful kola) is a medicinal plant that has been used worldwide as an alternative medication to promote human health. Comparative nephroprotective effects of crude seed powder, aqueous and methanolic extracts of B. coriacea in hepatotoxic rats was investigated for 56 days. The crude powder (BCCP), aqueous extract (BCAE) and methanolic extract (BCME) significantly reduced (p<0.05) the levels of creatinine and uric acid and decreased minimally throughout the treatment periods. Total protein increased significantly (p<0.05). Significant decrease (p<0.05) were observed with 200 mg/kg BCAE and BCCP respectively in urea and BUN levels. Noticeable nephroprotective effects may be attributed to the presence of phytochemicals like flavonoids and tannins which act as antioxidants. This study has demonstrated that B. coriacea crude seed powder, methanolic and aqueous extracts caused no adverse effect on the rat kidney and may be recommended for the management of nephrotoxicity Dhaka Univ. J. Pharm. Sci. 17(2): 227-235, 2018 (December)

scholarly journals An Alkenylresorcinol Derivative from Hakea Sericea Fruits and their Antimicrobial Activity

2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Ângelo Luís ◽  
Carla Cruz ◽  
Ana Paula Duarte ◽  
Fernanda Domingues
Keyword(s):  
Methanolic Extract ◽  
High Resolution Mass Spectrometry ◽  
Antibacterial Properties ◽  
Gram Negative Bacteria ◽  
Two Dimensional ◽  
Shrub Species ◽  
Phytochemical Composition ◽  
Bioassay Guided Fractionation ◽  
Mrsa Strains ◽  
Microtiter Assay

Hakea sericea has been introduced to Portugal for ornamental purposes. The phytochemical composition and the antioxidant, antibacterial, antibiofilm and cytotoxic properties of this shrub species have been previously reported. The present work describes the bioassay-guided fractionation of the crude methanolic extract of H. sericea fruits and the isolation of 9-(3,5-dihydroxy-4-methylphenyl)nona-3( Z)-enoic acid. The structure of this new compound was established by one- and two-dimensional NMR and IR spectroscopy, and high-resolution mass spectrometry. The antibacterial properties of the new alkenylresorcinol were studied by determining its MIC values against several strains of Gram-positive and Gram-negative bacteria using the resazurin microtiter assay. The new alkenylresorcinol inhibited the growth of Enterococcus faecalis, Listeria monocytogenes and Bacillus cereus with MIC values of 0.31, 0.02 and 0.16 mg/mL, respectively. Good MIC values were obtained against Staphylococcus aureus strains (0.005 – 0.16 mg/mL), including the clinical isolates (SA 01/10, SA 02/10 and SA 03/10) and MRSA strains.

Effects of BDE-99 on hormone homeostasis and biochemical parameters in adult male rats

2010 ◽  
Vol 48 (8-9) ◽  
pp. 2206-2211 ◽  
Author(s):  
Virginia Alonso ◽  
Victoria Linares ◽  
Montserrat Bellés ◽  
María Luisa Albina ◽  
Andreu Pujol ◽  
...  
Keyword(s):  
Adult Male ◽  
Biochemical Parameters ◽  
Male Rats ◽  
Hormone Homeostasis

The Effect of Different Doses of Green Tea Extract on Hematological and Biochemical Parameters in Adult Male Rats

2020 ◽  
Vol 11 (2) ◽  
pp. 1418
Author(s):  
Omer Mahrouf Ali Shoshin ◽  
Ahmed Abdulaali Azeez Baker ◽  
Evan Mohammed Mostafa ◽  
Noor Abdulaali Azeez Baker ◽  
Ahmad Salih Helal
Keyword(s):  
Green Tea ◽  
Adult Male ◽  
Biochemical Parameters ◽  
Green Tea Extract ◽  
Male Rats ◽  
Hematological And Biochemical Parameters ◽  
Tea Extract ◽  
Different Doses

Evaluation of Acute, Subacute and LD50 values of Methanolic extract of Sphaeranthus indicus leaves in Albino mice

2021 ◽  
pp. 2487-2492
Author(s):  
Gajendra Pratap Choudhary ◽  
Ashutosh Pal Jain
Keyword(s):  
Body Weight ◽  
Acute Toxicity ◽  
Biochemical Parameters ◽  
Methanolic Extract ◽  
The Body ◽  
Therapeutic Window ◽  
System Response ◽  
Acute Administration ◽  
Sphaeranthus Indicus ◽  
Hematological And Biochemical Parameters

Sphaeranthus indicus is one of the extremely precious herbs in the Indigenous System of Medicine. The present study was carried out to acute, subacute and LD50 values of methanolic extract of S. indicus leaves in Swiss mice of both sexes. The acute toxicity studies were conducted oral administration of 1.75, 5.5, 17.5, 55, 175, 550, 2000mg/kg body weight SIME used. Observations were recorded systemically up to 24 h after dose administration for behavior related to nervous system response or autonomic functions. Food and water intake, body weight variations, hematological and biochemical parameters were assessed. In sub acute toxicity treatment there were no significant variation in the body weights and haematological parameters except dose-dependent increase in lymphocyte count was noted in both sexes supported immunostimulant activity. Pathologically, significant protective effect on hepatic, renal functions and decreased cholesterol, triglyceride levels. The results did not show any treatment related abnormalities in terms of hematological and biochemical parameters in sub-acute toxicity. After acute administration, no mortality was recorded in mice treated with the SIME orally at a dose of 1000mg/kg. The LD50 values were determined using graphical method; we found a broad therapeutic window and a high therapeutic index value showed that the LD50 of the extract is 2480mg/kg. The results suggest that the plant seems to be high margin of drug safety in mice.

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