scholarly journals Diclofenac-induced cytotoxicity in cultured carp leukocytes

2020 ◽  
pp. S607-S618
Author(s):  
M Nemcova ◽  
J Pikula ◽  
J Zukal ◽  
V Seidlova
Keyword(s):  
Surface Waters ◽  
Aquatic Environment ◽  
Immune Functions ◽  
Medicinal Products ◽  
Carp Cyprinus Carpio ◽  
Cell Incubation ◽  
Different Temperatures ◽  
Higher Temperature ◽  
Incubation Temperatures ◽  
Concentration Cells

Diclofenac is a drug commonly used in human and veterinary medicine for the treatment of diseases associated with inflammation and pain. Medicinal products enter waste and surface waters on an everyday basis and contaminate the aquatic environment. Fish are therefore permanently exposed to these chemicals dissolved in their aquatic environment. To simulate variable environmental conditions, the aim of our study was to examine adverse effects of diclofenac under different temperatures of cell incubation (18, 21, 24, 27 and 30 °C). Cyto-toxic and -static effects of diclofenac in concentrations of 0.001 μg/ml, 0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml and 100 μg/ml for the carp (Cyprinus carpio) cultured leukocytes were quantified using detection of lactate dehydrogenase released from damaged cells. Overall DCF cytotoxicity was relatively low and its impact was pronounced at higher temperature and DCF concentration. Cells growth inhibition is changing more rapidly but it is high mainly at the highest concentration from low temperature. DNA fragmentation was not detected in tested leukocyte cell line. CYP450 increased diclofenac cytotoxicity only at the highest concentration but at incubation temperatures 18 and 27 °C. Leukocyte viability is essential for immune functions and any change can lead to reduction of resistance against pathogens, mainly in cold year seasons, when the immune system is naturally suppressed.

scholarly journals Effect of calcination temperature and calcination time on the kaolinite/tio2 composite for photocatalytic reduction of CO2

2012 ◽  
Vol 58 (4) ◽  
pp. 10-22 ◽  
Author(s):  
Martin Reli ◽  
Kamila Kočí ◽  
Vlastimil Matějka ◽  
Pavel Kovář ◽  
Lucie Obalová
Keyword(s):  
Crystallite Size ◽  
Physical Adsorption ◽  
Photocatalytic Reduction ◽  
Thermal Hydrolysis ◽  
Calcination Time ◽  
Different Temperatures ◽  
Higher Temperature ◽  
Titanyl Sulphate ◽  
Reduction Of Co2 ◽  
Hydrolysis Of

Abstract The kaolinite/TiO2 composite (60 wt% of TiO2) was prepared by thermal hydrolysis of a raw kaolin suspension in titanyl sulphate and calcined at different temperatures (600, 650 and 700°C) and for different times (1, 2 and 3 h). The obtained samples were characterized by XRPD, N2 physical adsorption and SEM, and tested for photocatalytic reduction of CO2. The different calcination conditions did not influence TiO2 phase composition, only slightly changed the specific surface area, and significantly affected crystallite size of kaolinite/TiO2 composite. A higher temperature and longer duration of calcination lead to higher crystallinity of the powder. The photocatalytic results showed that the crystallite size determined the efficiency of kaolinite/TiO2 photocatalysts.

scholarly journals Differences in some cranial bones between two Cyprinidae species, Common carp Cyprinus carpio (Linnaeus, 1758) and Crucian Carp Carassius carassius (Linnaeus, 1758) Collected from Tigris River, Iraq

2021 ◽  
Vol 15 (3) ◽  
pp. 225-237
Author(s):  
Muhammad Inad Ghazwan
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Aquatic Environment ◽  
Crucian Carp ◽  
Diagnostic Study ◽  
Carassius Carassius ◽  
Tigris River ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Crucian Carp Carassius Carassius

The present study attempts to identify some of the differences between the skull bones of two species Cyprinus carpio and Carassius carassius, which belong to the Cyprinidae family. The study is a taxonomic diagnostic study between the two species which are considered local fish abundant in the Iraqi aquatic environment

scholarly journals Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod <i>Limacina helicina</i>: mortality, shell degradation, and shell growth

2010 ◽  
Vol 7 (6) ◽  
pp. 8177-8214 ◽  
Author(s):  
S. Lischka ◽  
J. Büdenbender ◽  
T. Boxhammer ◽  
U. Riebesell
Keyword(s):  
Ocean Acidification ◽  
Surface Waters ◽  
Elevated Temperatures ◽  
Shell Growth ◽  
Shell Diameter ◽  
Partial Pressures ◽  
Limacina Helicina ◽  
Different Temperatures ◽  
Arctic Surface

Abstract. Due to their aragonitic shell thecosome pteropods may be particularly vulnerable to ocean acidification driven by anthropogenic CO2 emissions. This applies specifically to species inhabiting Arctic surface waters that are projected to become locally undersaturated with respect to aragonite as early as 2016. This study investigated the effects of rising pCO2 partial pressures and elevated temperature on pre-winter juveniles of the polar pteropod Limacina helicina. After a 29 days experiment in September/October 2009 at three different temperatures and under pCO2 scenarios projected for this century, mortality, shell degradation, shell diameter and shell increment were investigated. Temperature and pCO2 had a significant effect on mortality, but temperature was the overriding factor. Shell diameter, shell increment and shell degradation were significantly impacted by pCO2 but not by temperature. Mortality was 46% higher at 8 °C compared to 3 °C (in situ), and 14% higher at 1100 μatm CO2 as compared to 230 μatm CO2. Shell diameter and increment were reduced by 10% and 12% at 1100 μatm CO2 as compared to 230 μatm CO2, respectively, and shell degradation was 41% higher at elevated compared to ambient pCO2 partial pressures. We conclude that pre-winter juveniles will be negatively affected by both rising temperature and pCO2 which may result in a possible abundance decline of the overwintering population, the basis for next year's reproduction.

scholarly journals Synthesis of Fe3O4 and Fe2O3 nanoparticles using hybrid electrochemical-thermal method

2020 ◽  
Vol 55 (3) ◽  
pp. 221-228
Author(s):  
HA Simol ◽  
R Sultana ◽  
M Y A Mollah ◽  
MS Miran
Keyword(s):  
Iron Oxide ◽  
Total Pore Volume ◽  
Thermal Method ◽  
Prepared Compound ◽  
Xrd Pattern ◽  
Spectral Techniques ◽  
Different Temperatures ◽  
Ft Ir ◽  
Higher Temperature ◽  
Lower Temperature

Nanocrystalline Fe3O4 and Fe2O3 particles were successfully synthesized by an innovative hybrid electrochemical-thermal method. The as-prepared compound was calcined for an hour from 100 to 600oC temperatures. The crystallinity, morphology and chemical state of the synthesized powders were characterized by XRD, TG-DTA, SEM/EDS, FT-IR, and UV–Vis spectral techniques after calcinations. The Brunauer–Emmett–Teller (BET) plots confirmed that iron oxide nanoparticles (NPs) calcined at 400oC has a surface area of 18.28 m2 g-1 with a total pore volume of 0.2064 cc g-1. From XRD pattern it is revealed that the precursor calcined at lower temperature (100-400oC) correspond to Fe3O4,while the ones calcined at higher temperature follow Fe2O3 pattern. The morphology of iron oxide NPs calcined at different temperatures were studied with scanning electron microscope (SEM) and exhibits spherical shaped geometries with average diameters of 80-150nm. Bangladesh J. Sci. Ind. Res.55(3), 221-228, 2020

scholarly journals Hydrothermal Carbonization Brewer’s Spent Grains with the Focus on Improving the Degradation of the Feedstock

Energies ◽  
2018 ◽  
Vol 11 (11) ◽  
pp. 3226 ◽  
Author(s):  
Pablo Arauzo ◽  
Maciej Olszewski ◽  
Andrea Kruse
Keyword(s):  
Water Content ◽  
Liquid Product ◽  
Reaction Times ◽  
Hydrothermal Carbonization ◽  
Heating Value ◽  
High Heating Value ◽  
Acid Addition ◽  
Spent Grains ◽  
Different Temperatures ◽  
Higher Temperature

Hydrochar is a very interesting product from agricultural and food production residues. Unfortunately, severe conditions for complete conversion of lignocellulosic biomass is necessary, especially compared to the conversion of sugar compounds. The goal of this work is to improve the conversion of internal carbohydrates by application of a two-steps process, by acid addition and slightly higher water content. A set of experiments at different temperatures (180, 200, and 220 °C), reaction times (2 and 4 h), and moisture contents (80% and 90%) was performed to characterize the solid (high heating value (HHV), elemental) and liquid product phase. Afterwards, acid addition for a catalyzed hydrolysis reaction during hydrothermal carbonization (HTC) and a two-steps reaction (180 and 220 °C) were tested. As expected, a higher temperature leads to higher C content of the hydrochar and a higher fixed carbon (FC) content. The same effect was found with the addition of acids at lower temperatures. In the two-steps reaction, a primary hydrolysis step increases the conversion of internal carbohydrates. Higher water content has no significant effect, except for increasing the solubility of ash components.

scholarly journals Analysis of a Lab-Scale Heat Transformation Demonstrator Based on a Gas–Solid Reaction

Energies ◽  
2019 ◽  
Vol 12 (12) ◽  
pp. 2234 ◽  
Author(s):  
Jana Stengler ◽  
Julius Weiss ◽  
Marc Linder
Keyword(s):  
Experimental Studies ◽  
Hydration Reaction ◽  
Solid Reaction ◽  
Discharge Performance ◽  
Different Temperatures ◽  
Higher Temperature ◽  
Solid Bulk ◽  
Heat Transformation ◽  
Modelling Approach ◽  
Gas Solid Reaction

Heat transformation based on reversible chemical reactions has gained significant interest due to the high achievable output temperatures. This specific type of chemical heat pump uses a reversible gas–solid reaction, with the back and forward reactions taking place at different temperatures: by running the exothermic discharge reaction at a higher temperature than the endothermic charge reaction, the released heat is thermally upgraded. In this work, we report on the experimental investigation of the hydration reaction of strontium bromide (SrBr2) with regard to its use for heat transformation in the temperature range from 180 °C to 250 °C on a 1 kg scale. The reaction temperature is set by adjusting the pressure of the gaseous reactant. In previous experimental studies, we found the macroscopic and microscopic properties of the solid bulk phase to be subject to considerable changes due to the chemical reaction-. In order to better understand how this affects the thermal discharge performance of a thermochemical reactor, we combine our experimental work with a modelling approach. From the results of the presented studies, we derive design rules and operating parameters for a thermochemical storage module based on SrBr2.

Effect of Annealing on Microstructure of Hydroxyapatite Coatings and their Behaviours in Simulated Body Fluid

2014 ◽  
Vol 922 ◽  
pp. 657-662 ◽  
Author(s):  
Sharidah Azuar Abdul Azis ◽  
John Kennedy ◽  
Peng Cao
Keyword(s):  
Heat Treatment ◽  
Simulated Body Fluid ◽  
Body Fluid ◽  
Ion Beam ◽  
Apatite Formation ◽  
Ion Beam Sputtering ◽  
Different Temperatures ◽  
Higher Temperature ◽  
Xrd Patterns ◽  
Post Deposition

In this study, hydroxyapatite (HA) coatings on Ti6Al4V substrate were deposited using an ion beam sputtering technique. Owing to its medical applications, the crystalline phases present in the HA must be controlled. This study investigated the effect of post-deposition heat treatment at different temperatures and evaluated the microstructure of the HA coatings and their behaviours in simulated body fluid (SBF). The post-deposition treatment of the as-deposited samples was carried out in an air-circulated furnace at a temperature between 3000C and 6000C. The XRD patterns reveal that the minimum temperature to transform the HA coating from amorphous to crystalline phase is 4000C. A higher temperature at 6000C leads to a growth of the crystalline HA phases. Fourier transform infrared spectroscopy (FTIR) measurements show the existence of hydroxyl and PO-bonds in all coatings and the amounts varied with temperature. Atomic Force Microscopy (AFM) study suggests that the nanostructured crystalline HA starts to grow at 4000C and becomes more obvious at a higher temperature of 6000C. The simulated body fluid (SBF) test reveals that better apatite formation with post deposition heat treatment at 6000C would potentially enhance the formation of new bone (osseointegration).

Effect of Calcination Temperature on the Morphological and Phase Structure of Hydrothermally Synthesized Copper Ion Doped TiO2 Nanotubes

2014 ◽  
Vol 1024 ◽  
pp. 7-10 ◽  
Author(s):  
Mohd Hasmizam Razali ◽  
M.N. Ahmad-Fauzi ◽  
Abdul Rahman Mohamed ◽  
Srimala Sreekantan
Keyword(s):  
Calcination Temperature ◽  
Morphological Evolution ◽  
Copper Ion ◽  
X Ray Diffraction ◽  
X Ray ◽  
Transmission Electron ◽  
Different Temperatures ◽  
Scanning Electronmicroscopy ◽  
Higher Temperature ◽  
Uniform Diameter

Morphological evolution and phase transformations of copper ion doped TiO2nanotubes after being calcined at different temperatures were studied by field emission scanning electronmicroscopy, transmission electron microscopy, and X-ray diffraction. After calcination at 300°C, the nanotubes with uniform diameter and length wereobtained. At 400°C, the nanotube structures were maintained. Nevertheless the inner tube diameter became narrower, and in same instances disappeared due to aggregation of nanotubes. The copper ion doped TiO2nanotubes then transformed to nanorodsat 500°C and the length of the nanorodsshortens after calcination at 600 °C. When the calcination temperature was further increased to 700°C, the nanorodsdisintegrate to form nanoparticles. On the other hand the phase structures of copper ion doped TiO2nanotubes calcined at 300 and 400 °C were TiO2hexagonal. After calcined at higher temperature (600 and 700°C) they transformed to anatase TiO2(tetragonal).

Wetting and Interfacial Behaviors of Molten Ag, Cu and Ag-28Cu on WC-8Co Cemented Carbide

2016 ◽  
Vol 697 ◽  
pp. 555-560 ◽  
Author(s):  
Hao Fu ◽  
Xiang Zhao Zhang ◽  
Gui Wu Liu ◽  
Zi Wei Xu ◽  
Tie Zheng Pan ◽  
...  
Keyword(s):  
Contact Angle ◽  
Sessile Drop ◽  
Testing Temperature ◽  
Cemented Carbide ◽  
Equilibrium Contact Angle ◽  
Sessile Drop Technique ◽  
Different Temperatures ◽  
Higher Temperature ◽  
Drop Technique ◽  
Scanning Electron

The wetting and spreading of molten Cu, Ag and Ag-28Cu (wt.%) on the WC-Co cemented carbide were investigated by the sessile drop technique at different temperatures, and the interfacial behaviors of the metal/WC-Co couples were analyzed by scanning electron microscopy (SEM) coupled with energy dispersive spectroscopy (EDS). The experimental results showed that the testing temperature and the composition of drop can play a key role in the wetting and spreading, and the good wettability with contact angle of less than 40o can be obtained. Moreover, the strongest interactions can be produced between the molten Cu and the WC-Co substrate at the higher temperature of 1100 oC due to the strong interdiffusion and solid solution between Cu and Co, resulting in the lowest equilibrium contact angle of ~0o. In addition, smooth and clean interfaces, without formation of visible interfacial reaction layer, were observed in all the three wetting systems. This work may also provide guidance to brazing of the cemented carbide.

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