scholarly journals Prenatal Caffeine Damaged Learning and Memory in Rat Offspring Mediated by ARs/PKA/CREB/BDNF Pathway

2018 ◽  
pp. 975-983 ◽  
Author(s):  
Y. LI ◽  
W. ZHANG ◽  
R. SHI ◽  
M. SUN ◽  
L. ZHANG ◽  
...  
Keyword(s):  
Learning And Memory ◽  
Maze Learning ◽  
Adult Offspring ◽  
Sprague Dawley ◽  
Long Term Effects ◽  
Developmental Problems ◽  
A2a Receptors ◽  
Rat Offspring ◽  
Sprague Dawley Rats

Prenatal exposure to caffeine can cause developmental problems. This study determined chronic influence of prenatal caffeine at relatively higher doses on cognitive functions in the rat offspring. Pregnant Sprague-Dawley rats (4-month-old) were exposed to caffeine (20 mg/kg, twice a day) for whole pregnancy from gestational day 4. Fetal and offspring body and brain weight was measured. Learning and memory were tested in adult offspring with Morris water maze. Learning and memory-related receptors were measured. The exposure to prenatal caffeine not only caused fetal growth restriction, but also showed long-term effects on learning and memory in the offspring. The caffeine offspring exhibited longer escape latency and path length in navigation testing. The number of passing the target was significantly reduced in those offspring. The expression of adenosine A1 and A2A receptors, nuclear PKA Cα, Cβ subunits, and pCREB were significantly increased in the fetal and neonatal brain, and suppressed in the hippocampus of the adult offspring. The expression of BDNF and TrkB were reduced regardless of various ages. The results suggest that intrauterine programming dysfunction of adenosine receptors and the down-stream of cAMP/PKA/pCREB system may play an important role in prenatal caffeine induced cognition disorders in the adult offspring.

scholarly journals Learning and Memory Effects of Neonatal Methamphetamine Exposure in Sprague-Dawley Rats: Test of the Role of Dopamine Receptors D1 in Mediating the Long-Term Effects

2019 ◽  
Vol 41 (1-2) ◽  
pp. 44-55 ◽  
Author(s):  
Sarah A. Jablonski ◽  
Michael T. Williams ◽  
Charles V. Vorhees
Keyword(s):  
Learning And Memory ◽  
Water Maze ◽  
High Dose ◽  
Sprague Dawley ◽  
Long Term Effects ◽  
Learning Deficits ◽  
Sprague Dawley Rats ◽  
Cincinnati Water Maze ◽  
Locomotor Deficits

Methamphetamine (MA) abuse is a worldwide issue that produces health and cognitive effects in the user. MA is abused by some women who then become pregnant and expose their developing child to the drug. Preclinical rodent models demonstrate cognitive deficits following developmental MA exposure, an effect observed in children exposed to MA in utero. To determine if the dopamine receptor D1 (DRD1) is involved in the learning and memory deficits following MA exposure, male Sprague-Dawley rats were treated 4 times daily at 2 h intervals with 0 (saline) or 10 mg/kg of MA from postnatal day (P)6–15, 30 min after 0.5, 1.0, or 2.0 mg/kg SCH23390. Cincinnati water maze testing began on P30, and the high dose of SCH23390 blocked the learning deficits induced by MA with no effect from the lower doses. Morris water maze (MWM) learning deficits following MA were not protected by SCH23390, although there was a non-dose dependent effect in the acquisition phase. Locomotor deficits induced by MA were reversed by all doses of SCH23390. There were no effects of MA on criterion to trial passive avoidance. Taken together, these data show that behaviors that are dependent on the striatum are better protected with the DRD1 antagonist during MA treatment than the hippocampally mediated spatial learning in the MWM. This suggests that multiple mechanisms exist for the deficits induced by neonatal MA administration.

scholarly journals Developmental Fluoxetine Exposure Normalizes the Long-Term Effects of Maternal Stress on Post-Operative Pain in Sprague-Dawley Rat Offspring

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e57608 ◽  
Author(s):  
Liesbeth Knaepen ◽  
Ine Rayen ◽  
Thierry D. Charlier ◽  
Marianne Fillet ◽  
Virginie Houbart ◽  
...  
Keyword(s):  
Maternal Stress ◽  
Sprague Dawley ◽  
Long Term Effects ◽  
Rat Offspring ◽  
Sprague Dawley Rat ◽  
Post Operative Pain

Maternal exposure to low doses of bisphenol A affects learning and memory in male rat offspring with abnormal N-methyl-d-aspartate receptors in the hippocampus

2021 ◽  
pp. 074823372098462
Author(s):  
Chong Wang ◽  
Yao Shu ◽  
Li Xu ◽  
Qiling Liu ◽  
Bei Zhang ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Learning And Memory ◽  
Maternal Exposure ◽  
Male Offspring ◽  
Male Rat ◽  
Control Group ◽  
Sprague Dawley ◽  
Expression Levels ◽  
Rat Offspring ◽  
Sprague Dawley Rats

Bisphenol A (BPA), a component of polycarbonate and epoxy resins, has been reported to induce learning and memory deficits. However, the mechanisms have not been fully elucidated. Growing evidence has suggested that N-methyl-d-aspartate receptors (NMDARs) are involved in cognitive impairments. In this study, BPA was administered to female Sprague–Dawley rats (six per dose group) at concentrations of 0 (control), 4, 40, and 400 μg/kg·body weight/day from gestation day 1 through lactation day 21. Spatial learning was evaluated using the Morris water maze on postnatal day 22. Expression levels of NMDARs were determined using real-time polymerase chain reaction and Western blot. The results showed that male offspring exposed to BPA exhibited increased latency in reaching the platform and reduced time in the target quadrant, and the number of crossing the platform was less, as compared with the control group. The mRNA and protein expression levels of NMDARs in the hippocampus were significantly downregulated when compared with the control group of male offspring. The data showed that maternal exposure to BPA at low dosage can cause cognitive deficits in male rat offspring, probably due to a decrease in NMDARs in the hippocampus.

scholarly journals Periodicity During Hypercapnic and Hypoxic Stimulus Is Crucial in Distinct Aspects of Phrenic Nerve Plasticity

2016 ◽  
pp. 133-143 ◽  
Author(s):  
I. STIPICA ◽  
I. PAVLINAC DODIG ◽  
R. PECOTIC ◽  
Z. DOGAS ◽  
Z. VALIC ◽  
...  
Keyword(s):  
Phrenic Nerve ◽  
Sprague Dawley ◽  
Long Term Effects ◽  
Burst Frequency ◽  
Mechanically Ventilated ◽  
Sprague Dawley Rats ◽  
Pattern Sensitivity ◽  
Long Term Facilitation ◽  
Nerve Plasticity

This study was undertaken to determine pattern sensitivity of phrenic nerve plasticity in respect to different respiratory challenges. We compared long-term effects of intermittent and continuous hypercapnic and hypoxic stimuli, and combined intermittent hypercapnia and hypoxia on phrenic nerve plasticity. Adult, male, urethane-anesthetized, vagotomized, paralyzed, mechanically ventilated Sprague-Dawley rats were exposed to: acute intermittent hypercapnia (AIHc or AIHcO2), acute intermittent hypoxia (AIH), combined intermittent hypercapnia and hypoxia (AIHcH), continuous hypercapnia (CHc), or continuous hypoxia (CH). Peak phrenic nerve activity (pPNA) and burst frequency were analyzed during baseline (T0), hypercapnia or hypoxia exposures, at 15, 30, and 60 min (T60) after the end of the stimulus. Exposure to acute intermittent hypercapnia elicited decrease of phrenic nerve frequency from 44.25±4.06 at T0 to 35.29±5.21 at T60, (P=0.038, AIHc) and from 45.5±2.62 to 37.17±3.68 breaths/min (P=0.049, AIHcO2), i.e. frequency phrenic long term depression was induced. Exposure to AIH elicited increase of pPNA at T60 by 141.0±28.2 % compared to baseline (P=0.015), i.e. phrenic long-term facilitation was induced. Exposure to AIHcH, CHc, or CH protocols failed to induce long-term plasticity of the phrenic nerve. Thus, we conclude that intermittency of the hypercapnic or hypoxic stimuli is needed to evoke phrenic nerve plasticity.

Developmental exposure to paraquat and maneb can impair cognition, learning and memory in Sprague-Dawley rats

2016 ◽  
Vol 12 (10) ◽  
pp. 3088-3097 ◽  
Author(s):  
Bai Li ◽  
Xi He ◽  
Yan Sun ◽  
Baixiang Li
Keyword(s):  
Cognitive Function ◽  
Signaling Pathway ◽  
Learning And Memory ◽  
Sprague Dawley ◽  
Developmental Exposure ◽  
Rat Offspring ◽  
Sprague Dawley Rats

PQ–MB may affect the behavior and cognitive function of rat offspring through the cAMP–PKA–CREB signaling pathway.

scholarly journals Unexpected short- and long-term effects of chronic adolescent HU-210 exposure on emotional behavior

2021 ◽  
Author(s):  
Miguel Farinha-Ferreira ◽  
Nádia Rei ◽  
Jo&atildeo Fonseca-Gomes ◽  
Catarina Miranda-Lourenço ◽  
Paula Serr&atildeo ◽  
...  
Keyword(s):  
Cannabinoid Receptor ◽  
Emotional Behavior ◽  
Sprague Dawley ◽  
Long Term Effects ◽  
Negative Effects ◽  
Short Term ◽  
Receptor Agonists ◽  
Sprague Dawley Rats ◽  
Short And Long Term

Chronic adolescent cannabinoid receptor agonist exposure has been shown to lead to persistent increases in depressive-like behaviors. This has been a key obstacle to the development of cannabinoid-based therapeutics. However, most of the published work has been performed with only three compounds, namely ∆9-tetrahydrocannabinol, CP55,940 and WIN55,212-2. Hypothesizing that different compounds may lead to distinct outcomes, we herein used the highly potent CB1R/CB2R full agonist HU-210, and first aimed at replicating cannabinoid-induced long-lasting effects, by exposing adolescent female Sprague-Dawley rats to increasing doses of HU-210, for 11 days and testing them at adulthood, after a 30-day drug washout. Surprisingly, HU-210 did not significantly impact adult anxious- or depressive-like behaviors. We then tested whether chronic adolescent HU-210 treatment resulted in short-term (24h) alterations in depressive-like behavior. Remarkably, HU-210 treatment simultaneously induced marked antidepressant- and prodepressant-like responses, in the modified forced swim (mFST) and sucrose preference tests (SPT), respectively. Hypothesizing that mFST results were a misleading artifact of HU-210-induced behavioral hyperreactivity to stress, we assessed plasmatic noradrenaline and corticosterone levels, under basal conditions and following an acute swim-stress episode. Notably, we found that while HU-210 did not alter basal noradrenaline or corticosterone levels, it greatly augmented the stress-induced increase in both. Our results show that, contrary to previously studied cannabinoid receptor agonists, HU-210 does not induce persisting depressive-like alterations, despite inducing marked short-term increases in stress-induced reactivity. By showing that not all cannabinoid receptor agonists may induce long-term negative effects, these results hold significant relevance for the development of cannabinoid-based therapeutics.

Short- and long-term effects of neonatal hypo- and hyperthyroidism on coronary arterioles in rat

1996 ◽  
Vol 271 (5) ◽  
pp. H1746-H1754 ◽  
Author(s):  
M. I. Heron ◽  
K. Rakusan
Keyword(s):  
Inhibitory Effect ◽  
Cardiac Mass ◽  
Numerical Density ◽  
Sprague Dawley ◽  
Long Term Effects ◽  
Neonatal Hypothyroidism ◽  
Capillary Growth ◽  
Sprague Dawley Rats ◽  
Neonatal Hyperthyroidism

Neonatal hypo- and hyperthyroid effects on coronary arteriolar geometry were examined in newborn male Sprague-Dawley rats treated for 12 or 28 days with either triiodothyronine or propylthiouracil. Long-term effects were assessed in weaned rats 52 days after stopping treatment. Influence of both neonatal conditions was more pronounced after 28 days. Neonatal hyperthyroidism induced cardiac hypertrophy; neonatal hypothyroidism attenuated cardiac growth. Hyperthyroid rats had similar arteriolar and capillary numerical densities and arteriolar length density but significantly greater (P < 0.05) total arteriolar length than control. Hypothyroid rats had similar arteriolar numerical and length densities, greater capillary numerical density (P < 0.05), but markedly lower total arteriolar length (P < 0.01) than control. Results suggest that neonatal hyperthyroidism stimulates arteriolar and capillary growth, whereas neonatal hypothyroidism attenuates arteriolar but not capillary growth. After cessation of treatment, total arteriolar length in previously hyperthyroid rats did not change despite increased cardiac mass, whereas previously hypothyroid rats demonstrated marked increases in both cardiac mass and total arteriolar length (P < 0.01). These results indicate a lasting inhibitory effect of early hyperthyroidism on subsequent arteriolar growth.

Sign in / Sign up

Export Citation Format

Share Document