scholarly journals Radioimmunoassay of Chromogranin A and Free Metanephrines in Diagnosis of Pheochromocytoma

2017 ◽  
pp. S397-S408 ◽  
Author(s):  
R. BÍLEK ◽  
T. ZELINKA ◽  
P. VLČEK ◽  
J. DUŠKOVÁ ◽  
D. MICHALSKÝ ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Chromogranin A ◽  
Multiple Endocrine Neoplasia ◽  
Clinical Performance ◽  
Blood Samples ◽  
Tumor Mass ◽  
Clinical Sensitivity ◽  
Comparative Group ◽  
Edta Plasma ◽  
One Year

This work discusses the clinical performance of chromogranin A, free metanephrine and normetanephrine determination in plasma using a radioimmunoanalytical methods for the diagnosis of pheochromocytoma and paraganglioma. Blood samples were collected from 55 patients (46 pheochromocytomas, 9 paragangliomas). A sampling of biological materials was performed preoperatively and about one week, six months and one year after adrenal gland surgery. The comparative group without a diagnosis of pheochromocytoma/paraganglioma consisted of 36 pheochromocytoma/paraganglioma patients more than 4 months after adrenal gland surgery, and of 87 patients, 16 of them with multiple endocrine neoplasia, 9 with medullary and 5 with parafolicullar carcinoma of the thyroid gland. The rest were patients with various adrenal gland disorders. Chromogranin A, metanephrine and normetanephrine were determined in the EDTA-plasma using a radioimmunoassay kits Cisbio Bioassays, France and IBL International GmbH, Germany. Clinical sensitivity was 96 % for the combination of metanephrine and normetanephrine, and 93 % for chromogranin A. Clinical specificity was 100 % for the combination metanephrine and normetanephrine, and 96 % for chromogranin A. Falsely elevated levels of chromogranin A were observed in 1 patient with chronic renal insufficiency and 9 analyses were influenced by the administration of proton pump inhibitors. These results were excluded of CGA specificity. Both the combination of plasma free metanephrine, normetanephrine and chromogranin A as determined by radioimmunoassays, which are simple without the necessity of special laboratory material, are effective markers of pheochromocytoma or paraganglioma. Chromogranin A exerts association to malignity and all markers are associated with tumor mass.

scholarly journals Chromogranin A in the Laboratory Diagnosis of Pheochromocytoma and Paraganglioma

Cancers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 586 ◽  
Author(s):  
Bílek ◽  
Vlček ◽  
Šafařík ◽  
Michalský ◽  
Novák ◽  
...  
Keyword(s):  
Chromogranin A ◽  
Clinical Performance ◽  
Laboratory Diagnosis ◽  
Control Group ◽  
Neuroendocrine Neoplasms ◽  
Blood Samples ◽  
Clinical Sensitivity ◽  
Adrenergic Activity ◽  
One Year ◽  
Clinical Conditions

This work discusses the clinical performance of chromogranin A (CGA), a commonly measured marker in neuroendocrine neoplasms, for the diagnosis of pheochromocytoma/ paraganglioma (PPGL). Plasma CGA (cut-off value 150 µg/L) was determined by an immunoradiometric assay. Free metanephrine (cut-off value 100 ng/L) and normetanephrine (cut-off value 170 ng/L) were determined by radioimmunoassay. Blood samples were collected from PPGL patients preoperatively, one week, six months, one year and two years after adrenal gland surgery. The control patients not diagnosed with PPGL suffered from adrenal problems or from MEN2 and thyroid carcinoma. The clinical sensitivity in the PPGL group of patients (n = 71) based on CGA is 90% and is below the clinical sensitivity determined by metanephrines (97%). The clinical specificity based on all plasma CGA values after surgery (n = 98) is 99% and is the same for metanephrines assays. The clinical specificity of CGA in the control group (n = 85) was 92% or 99% using metanephrines tests. We can conclude that plasma CGA can serve as an appropriate complement to metanephrines assays in laboratory diagnosis of PPGL patients. CGA is elevated in PPGLs, as well as in other neuroendocrine or non-neuroendocrine neoplasia and under clinical conditions increasing adrenergic activity.

scholarly journals Deconjugated Urinary Metanephrine, Normetanephrine and 3-Methoxytyramine in Laboratory Diagnosis of Pheochromocytoma and Paraganglioma

2015 ◽  
pp. S313-S322 ◽  
Author(s):  
R. BÍLEK ◽  
T. ZELINKA ◽  
P. VLČEK ◽  
J. DUŠKOVÁ ◽  
D. MICHALSKÝ ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Clinical Performance ◽  
Chromatographic Determination ◽  
Laboratory Diagnosis ◽  
Control Group ◽  
Clinical Sensitivity ◽  
One Year ◽  
Plasma Free Metanephrines ◽  
Urinary Metanephrines

This work discusses the clinical performance of deconjugated metanephrine (MN), normetanephrine (NMN) and 3-methoxytyramine (3MT) determined in the basal first morning urine using a chromatographic method with electrochemical detection for the clinical diagnosis of pheochromocytoma (PHEO) and paraganglioma (PGL). Urine samples were collected from 44 patients (36 with PHEO, 8 with PGL) aged 54+/-17 (20-78) years (22 females, 22 males). A sampling of biological materials was performed preoperatively and about one week, six months and one year after adrenal gland surgery. The control group consisted of 34 PHEO/PGL patients more than 4 months after adrenal gland surgery. All subjects in the control group were without a diagnosis of PHEO or PGL. Clinical sensitivity was 55 % for MN, 64 % for NMN, 80 % for combination of both MN and NMN, and only 23 % for 3TM. Clinical specificity calculated from the control group was 93 % for MN, 95 % for NMN, 95 % for the combination MN and NMN, and 97 % for 3TM. Cut-off values for deconjugated metanephrines in the basal urine were 310 (MN), 690 (NMN) and 250 μg/l (3MT). Chromatographic determination of deconjugated urinary metanephrines, which is simple without the necessity of special laboratory material, can serve for the screening of PHEO or PGL patients. Urine NMN and 3MT exerts an association to malignity, and all markers are associated with tumor mass. However, the principal laboratory diagnosis of PHEO or PGL must be based on plasma-free metanephrines and plasma chromogranin A with better performance in the laboratory diagnosis of PHEO or PGL.

scholarly journals One-Year Clinical Evaluation of the Bonding Effectiveness of a One-Step, Self-Etch Adhesive in Noncarious Cervical Lesion Therapy

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Babacar Faye ◽  
Mouhamed Sarr ◽  
Khaly Bane ◽  
Adjaratou Wakha Aidara ◽  
Seydina Ousmane Niang ◽  
...  
Keyword(s):  
Clinical Performance ◽  
Retention Rate ◽  
Recall Rate ◽  
Acid Etching ◽  
Significant Difference ◽  
Usphs Criteria ◽  
One Step ◽  
One Year ◽  
The One ◽  
Self Etch

This study evaluated the one-year clinical performance of a one-step, self-etch adhesive (Optibond All-in-One, Kerr, CA, USA) combined with a composite (Herculite XRV Ultra, Kerr Hawe, CA, USA) to restore NCCLs with or without prior acid etching. Restorations performed by the same practitioner were evaluated at baseline and after 3, 6, and 12 months using modified USPHS criteria. At 6 months, the recall rate was 100%. The retention rate was 84.2% for restorations with prior acid etching, but statistically significant differences were observed between baseline and 6 months. Without acid etching, the retention rate was 77%, and no statistically significant difference was noted between 3 and 6 months. Marginal integrity (93.7% with and 87.7% without acid etching) and discoloration (95.3% with and 92.9% without acid etching) were scored as Alpha or Bravo, with better results after acid etching. After one year, the recall rate was 58.06%. Loss of pulp vitality, postoperative sensitivity, or secondary caries were not observed. After one year retention rate was of 90.6% and 76.9% with and without acid conditioning. Optibond All-in-One performs at a satisfactory clinical performance level for restoration of NCCLs after 12 months especially after acid etching.

scholarly journals The One-Year In Vivo Comparison of Lithium Disilicate and Zirconium Dioxide Inlays

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 3102
Author(s):  
Rini Behera ◽  
Lora Mishra ◽  
Darshan Devang Divakar ◽  
Abdulaziz A. Al-Kheraif ◽  
Naomi Ranjan Singh ◽  
...  
Keyword(s):  
Zirconium Dioxide ◽  
Clinical Performance ◽  
Lithium Disilicate ◽  
Study Groups ◽  
Resin Cement ◽  
Internal Surfaces ◽  
Colour Match ◽  
One Year ◽  
The One

The objective of the present study was to evaluate the one-year clinical performance of lithium disilicate (LD) and zirconium dioxide (ZrO2) class II inlay restorations. Thirty healthy individuals who met the inclusion criteria were enrolled for the study. The patients were randomly divided into two study groups (n = 15): LD (IPS e.max press) and ZrO2 (Dentcare Zirconia). In the ZrO2 group, the internal surfaces of the inlays were sandblasted and silanized with Monobond N (Ivoclar, Leichsteistein, Germany). In the LD group, the internal surfaces of the inlays were etched with 5% hydrofluoric acid. The ceramic inlays were cemented with self-cure resin cement (Multilink N). Clinical examinations were performed using modified United State Public Health Codes and Criteria (USPHS) after 2 weeks, 4 weeks, 6 months and 1 year. The one-year survival rate was evaluated. In total, one failure was observed in the ZrO2 group. The survival probability after 1 year for the ZrO2 inlays was 93%, and for the LD inlays was 100%, which was statistically insignificant. The differences between both groups for most USPHS criteria (except for colour match) were statistically insignificant. Within the imitations of the present study, the lithium disilicate- and zirconia dioxide-based inlays exhibited comparable clinical performances. However, the colour and translucency match was superior for the lithium disilicate restorations.

Clinical Performance and Physical Properties of Twelve Amalgam Alloys

1978 ◽  
Vol 57 (11-12) ◽  
pp. 983-988 ◽  
Author(s):  
J.W. Osborne ◽  
E.N. Gale ◽  
C.L. Chew ◽  
B.F. Rhodes ◽  
R.W. Phillips
Keyword(s):  
Compressive Strength ◽  
Physical Properties ◽  
Failure Rate ◽  
Clinical Performance ◽  
Physical Property ◽  
One Year

An assessment of the marginal failure rate of 1,041 restorations of twelve alloys was made at one year. In addition, physical property tests were conducted. A correlation was found between the clinical performance and creep (.79), flow (.62) and 24-hour compressive strength (.60).

scholarly journals BRUCELLAR UVEITIS IN EASTERN INDIA

2016 ◽  
Vol 9 (6) ◽  
pp. 196
Author(s):  
Anindita Sen ◽  
Parthajit Banerjee ◽  
Devarati Dutta ◽  
Manas Pal ◽  
Atanu Ray ◽  
...  
Keyword(s):  
Developing Countries ◽  
Developing Country ◽  
Health Problem ◽  
East Asian ◽  
Zoonotic Diseases ◽  
Asian Countries ◽  
Serological Tests ◽  
Blood Samples ◽  
Brucella Infection ◽  
One Year

Objective: Brucellosis- one of the major zoonotic diseases, still remains an uncontrolled problem, in regions of high endemicity. Ophthalmic brucellosis is not studied and overlooked in most developing countries.  Considering the severe outcome of undiagnosed ophthalmic brucellosis, in this paper we made attempts to find out whether this disease still remains a health problem in a South East Asian developing country, where study of this disease is largely neglected.  Methods This study was carried out over a one year period from January 2015 to December 2015. Blood samples were collected from clinically confirmed cases of uveitis and they were subjected to five serological and one genus specific molecular investigations, for the detection of Brucella infection.Results Out of 20 uveitis cases, 4 (20%) cases confirmed as brucellar uveitis, by serological tests followed by PCR confirmation. After treatment of brucellosis all the four patients were recovered uneventfully.Conclusion: Brucella infection involving the eye is still a significant problem in South East Asian countries; hence in all uveitis cases in this reason brucellosis should be excluded by available laboratory tests.Government of India (Dept. of Biotechnology the major zoonotic diseases, still remains an uncontrolled problem, in regions of high endemicity. Ophthalmic brucellosis is not studied and overlooked in most developing countries.  Considering the severe outcome of undiagnosed ophthalmic brucellosis, in this paper we made attempts to find out whether this disease still remains a health problem in a South East Asian developing country, where study of this disease is largely neglected.   Methods This study was carried out over a one year period from January 2015 to December 2015. Blood samples were collected from clinically confirmed cases of uveitis and they were subjected to five serological and one genus specific molecular investigations, for the detection of Brucella infection.Results Out of 20 uveitis cases, 4 (20%) cases confirmed as brucellar uveitis, by serological tests followed by PCR confirmation. After treatment of brucellosis all the four patients were recovered uneventfully.Conclusion: Brucella infection involving the eye is still a significant problem in South East Asian countries; hence in all uveitis cases in this reason brucellosis should be excluded by available laboratory tests.

scholarly journals A Comprehensive Comparison of DNA Extraction Using Fresh and Stored Bloods in Molecular Hematology Diagnostic Study

2018 ◽  
Vol 17 (3) ◽  
pp. 424-432
Author(s):  
Siti Nazihahasma Hassan ◽  
Suharni Mohamad ◽  
Rosline Hassan ◽  
Selamah Ghazali ◽  
Wan Suriana Wan Ab Rahman
Keyword(s):  
Medical Science ◽  
Pcr Amplification ◽  
Diagnostic Study ◽  
Fresh Blood ◽  
Blood Samples ◽  
Acceptable Alternative ◽  
Significant Difference ◽  
Comprehensive Comparison ◽  
One Year ◽  
Stored Blood

Objective: Blood is the main source of DNA in molecular biology. It provides a high DNA quality and quantity. In this study, we compare the quality and quantity of DNA isolated from stored blood that has been kept at -40°C for one-year to that of fresh blood.Materials and Methods: Twelve fresh and stored blood samples were randomly selected for this study. Nucleo Spin® Blood L kit was used to isolate the DNA from the samples. The integrity and intensity of DNA were examined through 1.6% agarose gel precast with SYBR® safe DNA stain. The DNA samples were further examined through PCR amplification and Sanger sequencing.Results: There was no significant difference in quality and quantity of isolated DNA from fresh blood and stored blood samples. The high intensity of an intact DNA band as well as the success in PCR amplification and sequencing are indicators of high DNA quality.Conclusion: Proper storage of patients’left-over whole blood sample at -40°C offers an acceptable alternative for DNA resources in molecular study.Bangladesh Journal of Medical Science Vol.17(3) 2018 p.424-432

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