scholarly journals Deconjugated Urinary Metanephrine, Normetanephrine and 3-Methoxytyramine in Laboratory Diagnosis of Pheochromocytoma and Paraganglioma

2015 ◽  
pp. S313-S322 ◽  
Author(s):  
R. BÍLEK ◽  
T. ZELINKA ◽  
P. VLČEK ◽  
J. DUŠKOVÁ ◽  
D. MICHALSKÝ ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Clinical Performance ◽  
Chromatographic Determination ◽  
Laboratory Diagnosis ◽  
Control Group ◽  
Clinical Sensitivity ◽  
One Year ◽  
Plasma Free Metanephrines ◽  
Urinary Metanephrines

This work discusses the clinical performance of deconjugated metanephrine (MN), normetanephrine (NMN) and 3-methoxytyramine (3MT) determined in the basal first morning urine using a chromatographic method with electrochemical detection for the clinical diagnosis of pheochromocytoma (PHEO) and paraganglioma (PGL). Urine samples were collected from 44 patients (36 with PHEO, 8 with PGL) aged 54+/-17 (20-78) years (22 females, 22 males). A sampling of biological materials was performed preoperatively and about one week, six months and one year after adrenal gland surgery. The control group consisted of 34 PHEO/PGL patients more than 4 months after adrenal gland surgery. All subjects in the control group were without a diagnosis of PHEO or PGL. Clinical sensitivity was 55 % for MN, 64 % for NMN, 80 % for combination of both MN and NMN, and only 23 % for 3TM. Clinical specificity calculated from the control group was 93 % for MN, 95 % for NMN, 95 % for the combination MN and NMN, and 97 % for 3TM. Cut-off values for deconjugated metanephrines in the basal urine were 310 (MN), 690 (NMN) and 250 μg/l (3MT). Chromatographic determination of deconjugated urinary metanephrines, which is simple without the necessity of special laboratory material, can serve for the screening of PHEO or PGL patients. Urine NMN and 3MT exerts an association to malignity, and all markers are associated with tumor mass. However, the principal laboratory diagnosis of PHEO or PGL must be based on plasma-free metanephrines and plasma chromogranin A with better performance in the laboratory diagnosis of PHEO or PGL.

scholarly journals Chromogranin A in the Laboratory Diagnosis of Pheochromocytoma and Paraganglioma

Cancers ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 586 ◽  
Author(s):  
Bílek ◽  
Vlček ◽  
Šafařík ◽  
Michalský ◽  
Novák ◽  
...  
Keyword(s):  
Chromogranin A ◽  
Clinical Performance ◽  
Laboratory Diagnosis ◽  
Control Group ◽  
Neuroendocrine Neoplasms ◽  
Blood Samples ◽  
Clinical Sensitivity ◽  
Adrenergic Activity ◽  
One Year ◽  
Clinical Conditions

This work discusses the clinical performance of chromogranin A (CGA), a commonly measured marker in neuroendocrine neoplasms, for the diagnosis of pheochromocytoma/ paraganglioma (PPGL). Plasma CGA (cut-off value 150 µg/L) was determined by an immunoradiometric assay. Free metanephrine (cut-off value 100 ng/L) and normetanephrine (cut-off value 170 ng/L) were determined by radioimmunoassay. Blood samples were collected from PPGL patients preoperatively, one week, six months, one year and two years after adrenal gland surgery. The control patients not diagnosed with PPGL suffered from adrenal problems or from MEN2 and thyroid carcinoma. The clinical sensitivity in the PPGL group of patients (n = 71) based on CGA is 90% and is below the clinical sensitivity determined by metanephrines (97%). The clinical specificity based on all plasma CGA values after surgery (n = 98) is 99% and is the same for metanephrines assays. The clinical specificity of CGA in the control group (n = 85) was 92% or 99% using metanephrines tests. We can conclude that plasma CGA can serve as an appropriate complement to metanephrines assays in laboratory diagnosis of PPGL patients. CGA is elevated in PPGLs, as well as in other neuroendocrine or non-neuroendocrine neoplasia and under clinical conditions increasing adrenergic activity.

scholarly journals Radioimmunoassay of Chromogranin A and Free Metanephrines in Diagnosis of Pheochromocytoma

2017 ◽  
pp. S397-S408 ◽  
Author(s):  
R. BÍLEK ◽  
T. ZELINKA ◽  
P. VLČEK ◽  
J. DUŠKOVÁ ◽  
D. MICHALSKÝ ◽  
...  
Keyword(s):  
Adrenal Gland ◽  
Chromogranin A ◽  
Multiple Endocrine Neoplasia ◽  
Clinical Performance ◽  
Blood Samples ◽  
Tumor Mass ◽  
Clinical Sensitivity ◽  
Comparative Group ◽  
Edta Plasma ◽  
One Year

This work discusses the clinical performance of chromogranin A, free metanephrine and normetanephrine determination in plasma using a radioimmunoanalytical methods for the diagnosis of pheochromocytoma and paraganglioma. Blood samples were collected from 55 patients (46 pheochromocytomas, 9 paragangliomas). A sampling of biological materials was performed preoperatively and about one week, six months and one year after adrenal gland surgery. The comparative group without a diagnosis of pheochromocytoma/paraganglioma consisted of 36 pheochromocytoma/paraganglioma patients more than 4 months after adrenal gland surgery, and of 87 patients, 16 of them with multiple endocrine neoplasia, 9 with medullary and 5 with parafolicullar carcinoma of the thyroid gland. The rest were patients with various adrenal gland disorders. Chromogranin A, metanephrine and normetanephrine were determined in the EDTA-plasma using a radioimmunoassay kits Cisbio Bioassays, France and IBL International GmbH, Germany. Clinical sensitivity was 96 % for the combination of metanephrine and normetanephrine, and 93 % for chromogranin A. Clinical specificity was 100 % for the combination metanephrine and normetanephrine, and 96 % for chromogranin A. Falsely elevated levels of chromogranin A were observed in 1 patient with chronic renal insufficiency and 9 analyses were influenced by the administration of proton pump inhibitors. These results were excluded of CGA specificity. Both the combination of plasma free metanephrine, normetanephrine and chromogranin A as determined by radioimmunoassays, which are simple without the necessity of special laboratory material, are effective markers of pheochromocytoma or paraganglioma. Chromogranin A exerts association to malignity and all markers are associated with tumor mass.

MO432COMPARATIVE ANALYSIS OF INDIRECT IMMUNOFLUORESCENCE AND ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR ANTI-PLA2R ASSESMENT IN PATIENTS WITH PRIMARY MEMBRANOUS NEPHROPATHY

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Olga Galkina ◽  
Evdokia Bogdanova ◽  
Irina Zubina ◽  
Elena Levykina ◽  
Alexei Smirnov
Keyword(s):  
Indirect Immunofluorescence ◽  
Membranous Nephropathy ◽  
Laboratory Diagnosis ◽  
Response To Therapy ◽  
Control Group ◽  
Course Of Disease ◽  
Phospholipase A2 Receptor ◽  
Gender And Age ◽  
Positive Results

Abstract Background and Aims Antibodies to M-type phospholipase A2 receptor (PLA2R-Ab) are considered to be a promising biomarker for laboratory diagnosis of primary membranous nephropathy (PMN) and may be useful in the evaluation of the response to therapy and CKD prognosis. The aim of the study was to compare two immunoassay methods – indirect immunofluorescence (IIF) and enzyme immunoassay (ELISA) for the determination of circulating PLA2R-Ab in patients with PMN. Method The study included 54 patients aged 55 (40-63) yrs. (M: F [33:21]) with PMN before treatment (n=16) and treated with immunosuppressive therapy (IST) (n=38), and apparently healthy individuals of the corresponding gender and age (n=10). Proteinuria and estimated glomerular filtration rate (eGFR) were determined in all participants. The levels of PLA2R-Ab were determined by IIF and quantitative/ semi-quantitative ELISA (EURUIMMUN AG test, Germany). In 16 PMN patients without treatment and 28 PMN patients treated with IST the level of PLA2R-Ab was measured one time and in 10 PMN patients treated IST – in dynamic, from 2 to 5 times. Statistical comparisons among groups were performed using Mann–Whitney U-test and Kruskal-Wallis H tests. The association between variables was estimated using Spearman’s coefficient. Sensitivity and specificity of the methods were calculated. Results The correlation coefficient between IIF and ELISA was 0.82 (p <0.005). There were more PLA2R-Ab-positive cases detected by ELISA, both before treatment (ELISA - 80%, IIF - 67%) and among patients treated with IST (ELISA - 63%, IIF - 50%). In control group, ELISA showed no positive results for PLA2R-Ab (specificity was 100%). The levels of proteinuria and eGFR were associated with autoantibodies determined by ELISA, both quantitative and semi-quantitative (proteinuria: r = 0.69, p = 0.001; eGFR: r = -0.38, p = 0.035) but not by IIF (proteinuria: r=0.33, p=0.061; eGFR: r=-0.26, p=0.082). The levels of PLA2R-Ab measured by ELISA correlated with the course of disease in patients treated with IST, while IIF did not show any dynamics is some cases. Conclusion Both quantitative and semi-quantitative ELISA were considered to be more preferable methods since the obtained results correlate with renal dysfunction and allow to assess the concentration of PLA2R-Ab in the course of disease more accurately, that may contribute to timely correction of treatment and improvement of outcome.

scholarly journals One-year clinical evaluation of IPS Empress CAD versus polished Celtra Duo ceramic Laminate veneers (randomized controlled clinical trial)

2021 ◽  
Vol 24 (3) ◽  
pp. 13p
Author(s):  
Hayat Elbanna ◽  
Mohammed Labib Zamzam ◽  
Jylan Fouad El-Guindy ◽  
Ahmed Soliman Idris
Keyword(s):  
Survival Rate ◽  
Fracture Resistance ◽  
Clinical Performance ◽  
Control Group ◽  
Marginal Adaptation ◽  
Anterior Teeth ◽  
Significant Difference ◽  
One Year ◽  
Laminate Veneers

Objective: To evaluate fracture resistance and survival rate of IPS Empress CAD versus Polished Celtra Duo ceramic laminate veneers. Material and Methods: Thirty-six ceramic laminate veneers were fabricated for maxillary anterior teeth. The patients were divided into two groups according to the material Group 1(control group) fabricated from IPS Empress CAD laminate veneers and group 2(intervention group) fabricated from Polished Celtra Duo laminate veneers. Standardized the same preparation with butt joint design and chamfer finish line located supra gingival were performed for all the teeth. The fabrication of the veneers was performed using Cad\Cam (Ceramill motion) machine, with software (Exocad). The veneers surfaces were treated and silanated according to the manufacture instruction of each ceramic and enamel surfaces were etched where total etch adhesive protocol was obeyed using BISCO. Follow up sessions were done every two months up to one year for each patient using dental probe and operator vision to evaluate the fracture, survival rate, marginal adaptation, sensitivity and caries. according to USPHS criteria (United States Public Health Service). This was performed by an experienced, blinded investigator. Results: Fracture resistance, marginal adaptation, retention, caries and sensitivity were evaluated according to the criteria of USPHS and we found there is no significant difference as both groups scaled zero score. Conclusion: Both IPS Empress Cad and Polished Celtra Duo laminate veneers revealed successful clinical performance in terms of fracture resistance, marginal adaptation, retention, and sensitivity after one year follow up period.   Keywords Ceramic laminate veneers; IPS Empress CAD; Celtra DUO; Clinical performance.

scholarly journals Candida Albicans and Non-Albicans Species as Etiological Agent of Vaginitis in Pregnant and Non-Pregnant Women

2010 ◽  
Vol 10 (1) ◽  
pp. 89-97 ◽  
Author(s):  
Mirela Babić ◽  
Mirsada Hukić
Keyword(s):  
Pregnant Women ◽  
Candida Species ◽  
Test Group ◽  
Vaginal Swab ◽  
Selective Medium ◽  
Control Group ◽  
One Year ◽  
Vaginal Candidosis ◽  
Microscopic Findings

Pregnancy represents a risk factor in the occurrence of vaginal candidosis. The objectives of our study were: to make determination of the microscopic findings of vaginal swab, frequency of Candida species in the culture of pregnant women and patients who are not pregnant, determine the Candida species in all cultures, and to determine the frequency and differences in the frequency of C. albicans and other non-albicans species. In one year study performed during 2006 year, we tested patients of Gynaecology and Obstetrics clinic of the Clinical Centre in Sarajevo and Gynaecology department of the General hospital in Sarajevo. 447 woman included in the study were separated in two groups: 203 pregnant (in the last trimester of pregnancy), and 244 non-pregnant woman in period of fertility. Each vaginal swab was examined microscopically. The yeast, number of colonies, and the species of Candida were determined on Sabouraud dextrose agar with presence of antibiotics. For determination of Candida species, we used germ tube test for detection of C. albicans, and cultivation on the selective medium and assimilation tests for detection of non-albicans species. The results indicated positive microscopic findings in the test group (40,9%), as well as greater number of positive cultures (46,8%). The most commonly detected species for both groups was C. albicans (test group 40.9% and control group 23,0%). The most commonly detected non-albicans species for the test group were C. glabrata (4,2 %) and C. krusei (3,2%), and for the control group were C. glabrata (3,2%) and C. parapsilosis (3,2%). The microscopic findings correlated with the number of colonies in positive cultures. In the test group, we found an increased number of yeasts (64,3%), and the pseudopyphae and blastopores by microscopic examination as an indication of infection. In the control group, we found a small number of yeasts (64,6%), in the form of blastopores, as an indication of the candida colonisation. Our results indicate that gravidity, as the risc factor for incidence of infection, has the significant role in the incidence of vaginal candidosis.

scholarly journals Characteristic of the urea-forming function of the liver of cats with chlamidiosis

2020 ◽  
pp. 72-74
Author(s):  
Alexey Yurievich Strugovshchikov ◽  
Petr Vladimirovich Smutnev ◽  
Nikolay Alexandrovich Pudovkin ◽  
Vladimir Vasilievich Salautin
Keyword(s):  
Total Protein ◽  
Bacterial Infections ◽  
Initial Level ◽  
Laboratory Diagnosis ◽  
Control Group ◽  
Upper Respiratory Tract ◽  
Formation Function ◽  
Upper Respiratory ◽  
Polymerase Chain

Currently, broad-spectrum antibiotics are widely used to treat bacterial infections. One of them is     Azitronit containing 10% of azithromycin. The main objective of the work was to study the urea formation function of the liver of cats with chlamydia and the effect of the preparation  Azitronit on it. All the animals studied were subjected to a clinical study according to the generally accepted method, in which special attention was paid to the condition of the organs that are most often affected by chlamydia - the eyes, mucous membranes of the external genital organs, and upper respiratory tract. Laboratory diagnosis was carried out by polymerase chain reaction. Determination of biochemical parameters on an IDEXX Catalist analyzer. It was found that in sick animals the content of total protein, urea, ammonia, glutamine and ornithine increases by 15.5%, 27.6%, 27.8%, 18.2% and 17.5%, respectively, compared with healthy ones. After treatment of animals, the studied indices decreased by 4.9% (total protein), 5.3% (urea), 18.3% (glutamine), 10% (ammonia) and 13.6% relative to the initial level (sick animals) (ornithine), but did not reach the animals of the control group and were higher by 11.1%, 23.5%, 11.7%, 10% and 4.5%, respectively. In the blood of cats with chlamydia patients, the content of a number of metabolites of the ornithine urea cycle increases, the activity of arginase and transamination enzymes increases, the content of total protein and ornithine, the concentration of ammonia, while increasing the concentration of urea, glutamine. After treatment with the drug Azitronit, the desired indices decrease, but do not reach the indices in healthy animals.

scholarly journals Determination of the aminotransferase activity in tissues of infected with ectoparasites yearling carp

2017 ◽  
Vol 19 (79) ◽  
Author(s):  
Yu. Loboiko ◽  
B. Barylo ◽  
O. Krushelnytska
Keyword(s):  
Skeletal Muscle ◽  
Skeletal Muscles ◽  
Control Group ◽  
First Year ◽  
Aminotransferase Activity ◽  
One Year ◽  
Mixed Infestation ◽  
Experimental Group ◽  
Alt Activity

The paper presents data on changes in the activity of amіnotransferase (aspartate aminotransferase – AST, alanine aminotransferase – ALT) in various organs and tissues of carp at different intensity of infestation with ectoparasites. The material for studying the activity of aminotransferases was one-year-old carp, spontaneously invasive with ectoparasites. For this purpose, twelve groups of fish were formed for 6 individuals in each, with a body weight of 38.0 ± 4.8 g. In four groups of fish (control and three experimental) for the defeat by ectoparasites L. cyprinacea, D. vastator and for mixed infestation. Our studies have shown that the activity of aminotransferases in hepatopancreas and skeletal muscles of carp significantly changes in the lesion of fish by lereneae. In particular, the activity of ALT in hepatopancreas was significantly higher compared to the control group. The tendency to increase was observed in tissues of skeletal muscles. In the study of AST activity, its growth in hepatopancreas and skeletal muscle was determined. For the defeat of fish by dactylosurus, the activity of aminotransferases in carp gills varies considerably. In particular, ALT activity in gills of fish of groups 3 and 4 was significantly higher compared to the control group. At the same time, the activity of ALT in the tissues of the hepatopancreas of the 4th experimental group was likely to increase. The tendency to increase is established in tissues of hepatopancreas and gills in the study of activity of AST. As a result of fish invasion by dactylosurus, the activity of AST in hepatopancreas increased by 1.2 times. At the same time, the growth of the AST index in the gills of the first year of carp in the 3rd and 4th groups was established. In the study of the activity of aminotransferases for mixed infestation, it was found that the activity of ALT and AST in tissues of hepatopancreas, skeletal muscles and gills of groups 2, 3 and 4 was significantly higher compared to the control group.

Equivalent discrimination among states of thyroid function by immunochemiluminimetric and immunoradiometric determination of thyrotropin

1993 ◽  
Vol 39 (3) ◽  
pp. 544-547 ◽  
Author(s):  
M Roden ◽  
P Nowotny ◽  
U Hollenstein ◽  
B Schneider ◽  
H Vierhapper ◽  
...  
Keyword(s):  
Thyroid Function ◽  
Operating Characteristic ◽  
Clinical Performance ◽  
Area Under The Curve ◽  
Immunoradiometric Assay ◽  
Clinical Sensitivity ◽  
Thyroid Function Testing ◽  
Sensitivity Specificity ◽  
Function Testing

Abstract New immunochemiluminometric assays (ICMA) of thyrotropin (TSH) have been reported to facilitate the diagnosis of hyperthyroidism. To compare the accuracy of ICMA-TSH with that of a conventional immunoradiometric assay (IRMA-TSH), we examined 115 consecutive patients of a thyroid outpatient clinic. On the basis of complete thyroid-function testing, including thyroliberin tests, the untreated patients (n = 89) were subclinically hyperthyroid (n = 21), subclinically hypothyroid (n = 13), or euthyroid (n = 55). The receiver-operating characteristic (ROC) curve, used for comparing the TSH values obtained by these two methods, was shifted to the left for the IRMA-TSH. The area under the curve was greater for IRMA-TSH than for ICMA-TSH (0.984 vs 0.869, respectively), which suggests equal or better clinical performance of IRMA-TSH vs ICMA-TSH in discriminating between hyperthyroidism and euthyroidism. Both assays displayed similar clinical sensitivity/specificity for evaluating thyroid function in hypothyroid and treated patients. We conclude that the ICMA-TSH tested is not more accurate than an IRMA in distinguishing between hyperthyroidism and euthyroidism.

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