scholarly journals The Effect of Rat Strain, Diet Composition and Feeding Period on the Development of a Nutritional Model of Non-Alcoholic Fatty Liver Disease in Rats

2011 ◽  
pp. 317-328 ◽  
Author(s):  
O. KUČERA ◽  
T. GARNOL ◽  
H. LOTKOVÁ ◽  
P. STAŇKOVÁ ◽  
Y. MAZUROVÁ ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Wistar Rats ◽  
Male Rats ◽  
Standard Diet ◽  
Sprague Dawley ◽  
Alcoholic Fatty Liver ◽  
Sprague Dawley Rats ◽  
Alcoholic Fatty Liver Disease

Non-alcoholic fatty liver disease (NAFLD) is an important cause of liver-related morbidity and mortality. The aim of this work was to establish and characterize a nutritional model of NAFLD in rats. Wistar or Sprague-Dawley male rats were fed ad libitum a standard diet (ST-1, 10 % kcal fat), a medium-fat gelled diet (MFGD, 35 % kcal fat) and a high-fat gelled diet (HFGD, 71 % kcal fat) for 3 or 6 weeks. We examined the serum biochemistry, the hepatic malondialdehyde, reduced glutathione (GSH) and cytokine concentration, the respiration of liver mitochondria, the expression of uncoupling protein-2 (UCP-2) mRNA in the liver and histopathological samples. Feeding with MFGD and HFGD in Wistar rats or HFGD in Sprague-Dawley rats induced small-droplet or mixed steatosis without focal inflammation or necrosis. Compared to the standard diet, there were no significant differences in serum biochemical parameters, except lower concentrations of triacylglycerols in HFGD and MFGD groups. Liver GSH was decreased in rats fed HFGD for 3 weeks in comparison with ST-1. Higher hepatic malondialdehyde was found in both strains of rats fed HFGD for 6 weeks and in Sprague-Dawley groups using MFGD or HFGD for 3 weeks vs. the standard diet. Expression of UCP-2 mRNA was increased in Wistar rats fed MFGD and HFGD for 6 weeks and in Sprague-Dawley rats using HFGD for 6 weeks compared to ST-1. The present study showed that male Wistar and Sprague-Dawley rats fed by HFGD developed comparable simple steatosis without signs of progression to non-alcoholic steatohepatitis under our experimental conditions.

scholarly journals Lupine (Lupinus angustifolius L.) Peptide Prevents Non-Alcoholic Fatty Liver Disease in High-Fat Diet-Induced Obese Mice

2019 ◽  
Author(s):  
Ana Lemus-Conejo ◽  
Elena Grao-Cruces ◽  
Rocio Toscano ◽  
Lourdes M Varela ◽  
Carmen Claro ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
High Fat Diet ◽  
Fatty Liver Disease ◽  
Lupinus Angustifolius ◽  
Standard Diet ◽  
Obese Mice ◽  
High Fat ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease

Bioactive peptides are related to the prevention and treatment of many diseases. GPETAFLR is an octapeptide which was isolated from lupine (Lupinus angustifolius L.) and showed anti-inflammatory properties. The aim of this study was to evaluate the potential activity of GPETAFLR to prevent non-alcoholic fatty liver disease (NAFLD) in high-fat diet (HFD)-induced obese mice. C57BL/6J mice were fed a standard diet or an HFD. Two of the groups fed the HFD diet were treated with GPETAFLR in their drinking water at 0,5 mg/kg/d or 1 mg/kg/d. To determine the ability of GPETAFLR to improve the onset and progression of NAFLD, histological studies, hepatic enzyme profile, inflammatory cytokine and lipid metabolism-related genes and proteins were analyzed. Our results suggest that HFD-induced inflammatory metabolic disorders were alleviated by treatment with GPETAFLR. In conclusion, dietary lupine consumption could repair HFD-induced hepatic damage, possibly via modifications in the liver’s lipid signalling pathways.

The effect of single clove Black garlic on the hemostasis status and lipid profile in male Sprague Dawley rats with non-alcoholic fatty liver disease

10.5219/1516 ◽  
2021 ◽  
Vol 15 ◽  
pp. 387-395
Author(s):  
Lili Nurmawati ◽  
Mohammad Sulchan ◽  
Siti Fatimah-Muis ◽  
Hery Djagat Purnomo ◽  
Kis Djamiatun ◽  
...  
Keyword(s):  
Liver Disease ◽  
Treatment Group ◽  
Fatty Liver Disease ◽  
Lipid Profiles ◽  
Control Group ◽  
Sprague Dawley ◽  
Alcoholic Fatty Liver ◽  
Sprague Dawley Rats ◽  
Alcoholic Fatty Liver Disease ◽  
Pai 1

Non-alcoholic fatty liver disease (NAFLD) patients have alteration in hemostasis components. Thus, including excess expression of plasminogen activator inhibitor-1 (PAI-1), causing fibrinolysis disorders; the majority of these patients are hypercoagulable state prone to thrombosis. Some evidence suggests that garlic and garlic supplements have antithrombotic and anti-inflammatory properties. Besides, garlic stimulates fibrinolytic activity and normalizes plasma lipid imbalances. Black garlic is processed garlic that is produced through natural aging at a controlled temperature (70 °C) and high humidity (90%) for several days, without other additives. This study aimed to prove the effect of single clove black garlic (SCBG) (Allium sativum Linn) on PAI-1 levels and lipid profiles of NAFLD rats induced by a high-fat fructose diet (HFFD) containing 1.25% cholesterol and 0.5% cholic acid.The rats were then divided into healthy control group (K1+); NAFLD control group without treatment (K2+); 0.9 mg simvastatin treatment group (K1-); 45 mg metformin treatment group (K2-); SCBG 450 mg per 200g BW (X1); 900 mg per 200 g BW (X2); and 1350 mg per 200 g BW (X3). All treatments were administered for 4 weeks via oral gavage. As a result, significant differences in PAI-1 levels and lipid profiles between groups after the administration (p = 0.001) were noted and also by simvastatin and metformin, respectively. There was a correlation between PAI-1 and lipid profile of SCBG treatment. In conclusion, the administration of SCBG (1350 mg per 200 g BB per day) for 4 weeks had a significant effect on PAI-1 levels, and the lipid profiles in Sprague Dawley rats modeled NAFLD (p = 0.001). SCBG has provided benefits that can be useful in the management of NAFLD but it’s not equivalent to medicine.

scholarly journals Adverse effects of chronic exposure to nonylphenol on non-alcoholic fatty liver disease in male rats

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0180218 ◽  
Author(s):  
Jie Yu ◽  
Xuesong Yang ◽  
Ya Luo ◽  
Xuefeng Yang ◽  
Mengxue Yang ◽  
...  
Keyword(s):  
Liver Disease ◽  
Adverse Effects ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Chronic Exposure ◽  
Male Rats ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease

scholarly journals ARGININE/CITRULLINE CYCLE CHANGES IN DIET-INDUCED RAT MODEL OF NON-ALCOHOLIC FATTY LIVER DISEASE

2020 ◽  
Vol 73 (6) ◽  
pp. 1087-1092
Author(s):  
Igor М. Skrypnyk ◽  
Ganna S. Maslova ◽  
Roman І. Skrypnyk ◽  
Oleksandr F. Gopko ◽  
Tetiana V. Lymanets
Keyword(s):  
Liver Disease ◽  
Fatty Liver ◽  
Rat Model ◽  
Fast Food ◽  
Fatty Liver Disease ◽  
Liver Homogenate ◽  
Arginase Activity ◽  
Male Rats ◽  
Alcoholic Fatty Liver ◽  
Alcoholic Fatty Liver Disease

The aim: to study the Arginine / Citrulline-cycle features on a diet-induced rat model of non-alcoholic fatty liver disease (NAFLD). Materials and methods: The studies were carried out on 20 white non-linear adult rats, including 10 (50%) males, 10 (50%) females, weighing 160-220 g. NAFLD was modeled by a 9-week fast food diet. The level of arginine and citrulline, and the arginase activity were investigated in the animals’ liver homogenates and in the blood. The morphological analysis of liver tissues changes was done. Results: NAFLD modeling using a 9-week fast food diet resulted in maximum weight gain in male rats (р<0.05). Female rats had 3 times more accumulation of intra-abdominal fat than male rats in the main group (р<0.05). Histopathologic liver examination confirmed NAFLD development in rats on a fast food diet during 9 weeks (р<0.05). NAFLD led to an increment of arginine level in the blood and liver homogenate in the main group compared to controls (р<0.05). NAFLD development was accompanied by a decrease in arginase activity and citrulline level in the blood and liver homogenate compared to control (р<0.05). Conclusions: The experimental rat model of NAFLD showed the Arginine / Citrulline cycle disorders, that were characterized by an increased arginine level, a decreased arginase activity and citrulline concentration in the blood and liver tissues. KEY WORDS: non-alcoholic fatty liver disease, arginine, arginase, citrulline

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