scholarly journals Improvement of in vitro food allergy diagnostics by identifying unique antibody traits

10.33540/190 ◽  
2021 ◽  
Author(s):  
◽  
Anna Maria Ehlers
Keyword(s):  
Food Allergy ◽  
Allergy Diagnostics

scholarly journals A Proof-of-Concept of Label-Free Biosensing System for Food Allergy Diagnostics in Biophotonic Sensing Cells: Performance Comparison with ImmunoCAP

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2686 ◽  
Author(s):  
Rocio Espinosa ◽  
María Laguna ◽  
Fátima Fernández ◽  
Beatriz Santamaria ◽  
Francisco Sanza ◽  
...  
Keyword(s):  
Food Allergy ◽  
Performance Comparison ◽  
Common Disease ◽  
In Vitro Test ◽  
Label Free ◽  
Children And Young People ◽  
Allergy Diagnostics ◽  
Vitro Test ◽  
Biosensing System

Food allergy is a common disease worldwide with over 6% of the population (200–250 million people) suffering from any food allergy nowadays. The most dramatic increase seems to be happening in children and young people. Therefore, improvements in the diagnosis efficiency of these diseases are needed. Immunoglobulin type E (IgE) biomarker determination in human serum is a typical in vitro test for allergy identification. In this work, we used a novel biosensor based on label-free photonic transducers called BICELLs (Biophotonic Sensing Cells) for IgE detection. These BICELLs have a thin film of nitrocellulose over the sensing surface, they can be vertical optically interrogated, and are suitable for being integrated on a chip. The BICELLs sensing surface sizes used were 100 and 800 µm in diameter. We obtained calibration curves with IgE standards by immobilizating anti-IgE antibodies and identified with standard IgE calibrators in minute sample amounts (3 µL). The results, in similar assay format, were compared with commercially available ImmunoCAP®. The versatility of the interferometric nitrocellulose-based sensing surface was demonstrated since the limit of detections for BICELLs and ImmunoCAP® were 0.7 and 0.35 kU/L, respectively.

scholarly journals Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
C. Gómez-Casado ◽  
M. Garrido-Arandia ◽  
P. Gamboa ◽  
N. Blanca-López ◽  
G. Canto ◽  
...  
Keyword(s):  
Food Allergy ◽  
Ex Vivo ◽  
Binding Capacity ◽  
Cross Reactivity ◽  
T Cell Epitopes ◽  
Native Form ◽  
Ige Binding ◽  
Pru P 3

Nowadays, treatment of food allergy only considered the avoidance of the specific food. However, the possibility of cross-reactivity makes this practice not very effective. Immunotherapy may exhibit as a good alternative to food allergy treatment. The use of hypoallergenic molecules with reduced IgE binding capacity but with ability to stimulate the immune system is a promising tool which could be developed for immunotherapy. In this study, three mutants of Pru p 3, the principal allergen of peach, were produced based on the described mimotope and T cell epitopes, by changing the specific residues to alanine, named asPru p 3.01, Pru p 3.02, andPru p 3.03.Pru p 3.01showed very similar allergenic activity as the wild type byin vitroassays. However,Pru p 3.02andPru p 3.03presented reduced IgE binding with respect to the native form, byin vitro,ex vivo,and in vivo assays. In addition,Pru p 3.03had affected the IgG4 binding capacity and presented a random circular dichroism, which was reflected in the nonrecognition by specific antibodies anti-Pru p 3. Nevertheless, bothPru p 3.02andPru p 3.03maintained the binding to IgG1 and their ability to activate T lymphocytes. Thus,Pru p 3.02andPru p 3.03could be good candidates for potential immunotherapy in peach-allergic patients.

The risk of sensitization to furry animals in patients already sensitized to cat/dog: An in vitro evaluation using molecular-based allergy diagnostics

2015 ◽  
Vol 135 (6) ◽  
pp. 1664-1666 ◽  
Author(s):  
Gennaro Liccardi ◽  
Antonio Meriggi ◽  
Maria Russo ◽  
Stefania Croce ◽  
Antonello Salzillo ◽  
...  
Keyword(s):  
In Vitro Evaluation ◽  
Allergy Diagnostics

scholarly journals Diagnosis of food allergy: History, examination and in vivo and in vitro tests

2014 ◽  
Vol 105 (1) ◽  
pp. 69
Author(s):  
D A Van der Spuy ◽  
A J Terblanche ◽  
S Karabus ◽  
M Kriel ◽  
A I Manjra ◽  
...  
Keyword(s):  
Food Allergy ◽  
In Vitro Tests

Soybean Isoflavones Suppress Basophil Degranulation in Vitro in Subjects with Food Allergy

2013 ◽  
Vol 131 (2) ◽  
pp. AB119
Author(s):  
Mohanapriya Kamalakannan ◽  
Matthew Stadler ◽  
Hugh A. Sampson ◽  
Madhan Masilamani
Keyword(s):  
Food Allergy ◽  
Soybean Isoflavones

scholarly journals Flow cytometric analysis of in vitro activated basophils, specific IgE and skin tests in the diagnosis of pollen-associated food allergy

2005 ◽  
Vol 64B (1) ◽  
pp. 28-33 ◽  
Author(s):  
Didier G. Ebo ◽  
Margo M. Hagendorens ◽  
Chris H. Bridts ◽  
Annemie J. Schuerwegh ◽  
Luc S. De Clerck ◽  
...  
Keyword(s):  
Food Allergy ◽  
Flow Cytometric Analysis ◽  
Specific Ige ◽  
Skin Tests ◽  
Flow Cytometric
Sign in / Sign up

Export Citation Format

Share Document