scholarly journals Phylogeography of the invasive polychaete Sabella spallanzanii (Sabellidae) based on the nucleotide sequence of internal transcribed spacer 2 (ITS2) of nuclear rDNA

2001 ◽  
Vol 215 ◽  
pp. 169-177 ◽  
Author(s):  
FP Patti ◽  
MC Gambi
Keyword(s):  
Nucleotide Sequence ◽  
Internal Transcribed Spacer ◽  
Nuclear Rdna ◽  
Internal Transcribed Spacer 2

scholarly journals The new Internal Transcribed Spacer 2 diagnostic tool clarifies the taxonomic position and geographic distribution of the North American malaria vector Anopheles punctipennis

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
James M. Hodge ◽  
Andrey A. Yurchenko ◽  
Dmitriy A. Karagodin ◽  
Reem A. Masri ◽  
Ryan C. Smith ◽  
...  
Keyword(s):  
Malaria Vector ◽  
Internal Transcribed Spacer ◽  
Single Species ◽  
Its2 Sequence ◽  
Pathogen Transmission ◽  
Molecular Diagnostic ◽  
Diagnostic Tools ◽  
Sequence Length ◽  
Phylogenetic Position ◽  
Internal Transcribed Spacer 2

Abstract Background The malaria mosquito Anopheles punctipennis, a widely distributed species in North America, is capable of transmitting human malaria and is actively involved in the transmission of the ungulate malaria parasite Plasmodium odocoilei. However, molecular diagnostic tools based on Internal Transcribed Spacer 2 (ITS2) of ribosomal DNA are lacking for this species. Anopheles punctipennis is a former member of the Anopheles maculipennis complex but its systematic position remains unclear. Methods In this study, ITS2 sequences were obtained from 276 An. punctipennis specimens collected in the eastern and midwestern United States and a simple and robust Restriction Fragment Length Polymorphism approach for species identification was developed. The maximum-likelihood phylogenetic tree was constructed based on ITS2 sequences available through this study and from GenBank for 20 species of Anopheles. Results The analysis demonstrated a consistent ITS2 sequence length and showed no indications of intragenomic variation among the samples based on ITS2, suggesting that An. punctipennis represents a single species in the studied geographic locations. In this study, An. punctipennis was found in urban, rural, and forest settings, suggesting its potential broad role in pathogen transmission. Phylogeny based on ITS2 sequence comparison demonstrated the close relationship of this species with other members of the Maculipennis group. Conclusions This study developed molecular tools based on ITS2 sequences for the malaria vector An. punctipennis and clarified the phylogenetic position of the species within the Maculipennis group.

Nucleotide sequence of the internal transcribed spacer region of rDNA in mountain rye,Secale montanum Guss. (Gramineae)

1992 ◽  
Vol 20 (1) ◽  
pp. 161-162 ◽  
Author(s):  
K. H. Asay ◽  
C. Hsiao ◽  
N. J. Chatterton ◽  
K. B. Jensen ◽  
R. R-C. Wang
Keyword(s):  
Nucleotide Sequence ◽  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Region ◽  
Secale Montanum

scholarly journals Rhizospheric Microbiome Profiling of Capsicum annuum L. Cultivated in Amended Soils by 16S and Internal Transcribed Spacer 2 rRNA Amplicon Metagenome Sequencing

2017 ◽  
Vol 5 (30) ◽  
Author(s):  
Ali Asaff-Torres ◽  
Mariana Armendáriz-Ruiz ◽  
Manuel Kirchmayr ◽  
Raúl Rodríguez-Heredia ◽  
Marcos Orozco ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Internal Transcribed Spacer ◽  
Root Exudate ◽  
Microbial Consortium ◽  
Amended Soils ◽  
Internal Transcribed Spacer 2 ◽  
Capsicum Annuum L ◽  
Metagenome Sequencing ◽  
Microbiome Profiling

ABSTRACT Rhizospheric microbiomes of Capsicum annuum L. cultivated either conventionally or amended with a synthetic microbial consortium or a root exudate inductor, were characterized by 16S/internal transcribed spacer 2 (ITS2) rRNA amplicon metagenome sequencing. The most abundant taxa found, although differently represented in each treatment, were Gammaproteobacteria, Alphaproteobacteria, Actinobacteria, and Bacilli, as well as Chytridiomycetes and Mortierellomycotina.

scholarly journals Internal transcribed spacer 2 (ITS2) barcodes: A useful tool for identifying ChineseZanthoxylum

2018 ◽  
Vol 6 (6) ◽  
pp. e01157 ◽  
Author(s):  
Li-Li Zhao ◽  
Shi-Jing Feng ◽  
Jie-Yun Tian ◽  
An-Zhi Wei ◽  
Tu-Xi Yang
Keyword(s):  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer 2
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