scholarly journals Characterization of Neoparamoeba pemaquidensis strains: PCR-RFLP of the internal transcribed spacer region from the amoeba and endosymbiont

2007 ◽  
Vol 76 ◽  
pp. 141-149 ◽  
Author(s):  
CGB Caraguel ◽  
N Donay ◽  
Jr Frasca S ◽  
CJ O’Kelly ◽  
RJ Cawthorn ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Region ◽  
Pcr Rflp

scholarly journals Overview of Agaricus Subrufescens Strains used in the Last 15 Years in Brazil and other Countries and Current Potential Materials for the Future

2021 ◽  
Author(s):  
Diego Cunha Zied ◽  
Wagner G. Vieira Junior ◽  
Douglas M. M. Soares ◽  
Cassius V. Stevani ◽  
Eustáquio S. Dias ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
High Weight ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Agaricus Blazei ◽  
Internal Transcribed Spacer Region ◽  
Commercial Use ◽  
Agaricus Subrufescens ◽  
Current Potential

Abstract The mushroom Agaricus subrufescens has been synonymous with Agaricus blazei and Agaricus brasiliensis during the last decades and there has been much discussion with regards to the origin, distribution, and nomenclature of this mushroom. Therefore, we conducted a genetic and morphological characterization of the mycelium and mushroom of four commercial strains currently cultivated in Brazil (ABL CS7, ABL 18/01, ABL 98/11, and ABL 16/01) together with an assessment of their agronomic behavior and compared these results with those of other strains used during the last 15 years. All the A. subrufescens strains characterized here are phylogenetically related to the Americas/Europe specimens, bearing an internal transcribed spacer region of type A (ABL 16/01) or both types A and B (ABL 18/01, ABL 98/11, and ABL CS7). We did not find any correlation between the morphological characteristics of the mycelial colonies and the agronomic behavior of the strains. Strains ABL 98/11 and ABL 16/01 produced the best yields and morphological characteristics for the mushrooms, indicating their high weight, which enhances the commercialization of the mushroom and justifies their longstanding commercial use over the last 15 years.

Identification of Monilia species in Tibet and characterization of M. yunnanensis in China

Plant Disease ◽  
2021 ◽  
Author(s):  
Liangfen Yin ◽  
Song Huang ◽  
Qin Tan ◽  
Yang Zhou ◽  
Guoqing Li ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Differentiation ◽  
Internal Transcribed Spacer ◽  
Brown Rot ◽  
Stone Fruits ◽  
Morphological Investigation ◽  
Internal Transcribed Spacer Region ◽  
Germ Tubes ◽  
Different Parts

Samples of peach and plum fruits with brown rot symptoms were collected from Tibet in 2019 and 2020, and the causal agent was identified as Monilia yunnanensis, which represents the first characterization of Monilia spp. on peach and plum in Tibet. Morphological investigation showed that some conidia from naturally diseased fruits were larger than those observed in previously isolated M. yunnanensis. Some conidia of M. yunnanensis isolates from Tibet produced more than two, even up to six germ tubes from different parts of each conidium, instead of one or two germ tubes developing from the pointy sides of each conidium. The alignment of ribosomal internal transcribed spacer region (ITS) sequences revealed that some isolates from Tibet displayed a mutation at the 374th position from adenine (A) to cytosine (C). Although above-mentioned differences were observed between isolates from Tibet and other regions, phylogenetic analysis indicated that all of the M. yunnanensis isolates from different stone fruits and different regions in China were clustered together without obvious genetic differentiation. These results revealed that hosts and geographical environments did not play a major role in the evolution of M. yunnanensis.

Genetic diversity among a complex of cereal cyst nematodes inferred from RFLP analysis of the ribosomal internal transcribed spacer region

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 479-486 ◽  
Author(s):  
S. Bekal ◽  
J. P. Gauthier ◽  
R. Rivoal
Keyword(s):  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
Rflp Analysis ◽  
Restriction Pattern ◽  
Nuclear Ribosomal Dna ◽  
Heterodera Avenae ◽  
Cyst Nematodes ◽  
Internal Transcribed Spacer Region ◽  
5.8S Rdna ◽  
Pcr Rflp

This study examined the restriction polymorphism (RFLP) of the nuclear ribosomal DNA in Heterodera avenae, H. filipjevi, H. mani, H. latipons, and the taxonomically unclear Gotland strain in order to establish a molecular characterization and phylogenetic relationships in the complex of cereal cyst nematodes (CCN). The internal transcribed spacer (ITS) and 5.8S rDNA were amplified by PCR from a single female or a cyst of 27 different geographic isolates of the CCN complex and one population of H. schachtii, used as outgroup. The amplified product was 1.2 kb long and 14 of 15 enzymes produced restriction fragments for each isolate. Relationships between populations were determined from UPGMA analysis based on distance values calculated from RFLP data. Digestions with TaqI clearly differentiated H. avenae, H. latipons, and a group composed of H. filipjevi and the Gotland strain. Six endonucleases (HaeIII, HinfI, ItaI, PstI, TaqI, and Tru9I) produced the same restriction pattern with H. filipjevi and the Gotland strain, and both were clearly separated from H. avenae with PstI. Restriction sites have revealed a mixture of the species H. latipons and H. avenae, and possible infraspecific variation in H. avenae. The inferred phylogenetic relationships of species in the CCN complex are in agreement with their morphological characterization.Key words: cereal cyst nematodes, Heterodera avenae, PCR, RFLP, ribosomal diversity.

Sign in / Sign up

Export Citation Format

Share Document