scholarly journals Characterization of fungi in office dust: Comparing results of microbial secondary metabolites, fungal internal transcribed spacer region sequencing, viable culture and other microbial indices

Indoor Air ◽  
2018 ◽  
Vol 28 (5) ◽  
pp. 708-720 ◽  
Author(s):  
J.-H. Park ◽  
M. Sulyok ◽  
A. R. Lemons ◽  
B. J. Green ◽  
J. M. Cox-Ganser
Keyword(s):  
Secondary Metabolites ◽  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Region ◽  
Viable Culture ◽  
Microbial Indices

scholarly journals Overview of Agaricus Subrufescens Strains used in the Last 15 Years in Brazil and other Countries and Current Potential Materials for the Future

2021 ◽  
Author(s):  
Diego Cunha Zied ◽  
Wagner G. Vieira Junior ◽  
Douglas M. M. Soares ◽  
Cassius V. Stevani ◽  
Eustáquio S. Dias ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
High Weight ◽  
Morphological Characteristics ◽  
Morphological Characterization ◽  
Agaricus Blazei ◽  
Internal Transcribed Spacer Region ◽  
Commercial Use ◽  
Agaricus Subrufescens ◽  
Current Potential

Abstract The mushroom Agaricus subrufescens has been synonymous with Agaricus blazei and Agaricus brasiliensis during the last decades and there has been much discussion with regards to the origin, distribution, and nomenclature of this mushroom. Therefore, we conducted a genetic and morphological characterization of the mycelium and mushroom of four commercial strains currently cultivated in Brazil (ABL CS7, ABL 18/01, ABL 98/11, and ABL 16/01) together with an assessment of their agronomic behavior and compared these results with those of other strains used during the last 15 years. All the A. subrufescens strains characterized here are phylogenetically related to the Americas/Europe specimens, bearing an internal transcribed spacer region of type A (ABL 16/01) or both types A and B (ABL 18/01, ABL 98/11, and ABL CS7). We did not find any correlation between the morphological characteristics of the mycelial colonies and the agronomic behavior of the strains. Strains ABL 98/11 and ABL 16/01 produced the best yields and morphological characteristics for the mushrooms, indicating their high weight, which enhances the commercialization of the mushroom and justifies their longstanding commercial use over the last 15 years.

Identification of Monilia species in Tibet and characterization of M. yunnanensis in China

Plant Disease ◽  
2021 ◽  
Author(s):  
Liangfen Yin ◽  
Song Huang ◽  
Qin Tan ◽  
Yang Zhou ◽  
Guoqing Li ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Differentiation ◽  
Internal Transcribed Spacer ◽  
Brown Rot ◽  
Stone Fruits ◽  
Morphological Investigation ◽  
Internal Transcribed Spacer Region ◽  
Germ Tubes ◽  
Different Parts

Samples of peach and plum fruits with brown rot symptoms were collected from Tibet in 2019 and 2020, and the causal agent was identified as Monilia yunnanensis, which represents the first characterization of Monilia spp. on peach and plum in Tibet. Morphological investigation showed that some conidia from naturally diseased fruits were larger than those observed in previously isolated M. yunnanensis. Some conidia of M. yunnanensis isolates from Tibet produced more than two, even up to six germ tubes from different parts of each conidium, instead of one or two germ tubes developing from the pointy sides of each conidium. The alignment of ribosomal internal transcribed spacer region (ITS) sequences revealed that some isolates from Tibet displayed a mutation at the 374th position from adenine (A) to cytosine (C). Although above-mentioned differences were observed between isolates from Tibet and other regions, phylogenetic analysis indicated that all of the M. yunnanensis isolates from different stone fruits and different regions in China were clustered together without obvious genetic differentiation. These results revealed that hosts and geographical environments did not play a major role in the evolution of M. yunnanensis.

scholarly journals Characterization of Neoparamoeba pemaquidensis strains: PCR-RFLP of the internal transcribed spacer region from the amoeba and endosymbiont

2007 ◽  
Vol 76 ◽  
pp. 141-149 ◽  
Author(s):  
CGB Caraguel ◽  
N Donay ◽  
Jr Frasca S ◽  
CJ O’Kelly ◽  
RJ Cawthorn ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Region ◽  
Pcr Rflp

Morphological and Molecular Characterization of Sclerotinia sclerotiorum on Sugar Beet in Montana, USA

2021 ◽  
Author(s):  
Mohamed F. R Khan ◽  
Md. Ziaur Rahman Bhuyian ◽  
Dilip Lashman ◽  
Presley Mosher ◽  
Timothy Cymbaluk ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Sugar Beet ◽  
Sclerotinia Sclerotiorum ◽  
Internal Transcribed Spacer ◽  
Economic Activities ◽  
Internal Transcribed Spacer Region ◽  
Molecular Identity ◽  
South Central ◽  
Morphological And Molecular Characterization

In this contribution, we report on a survey of leaf blight on sugar beet in the Sidney factory district of Montana caused by the soilborne fungus Sclerotinia sclerotiorum. In addition to morphological and pathological characterization of the pathogen on sugar beet, we confirmed the molecular identity of the fungus by DNA sequence analysis of the ribosomal Internal Transcribed Spacer region. Our findings are significant because the sugar beet production in south-central and eastern Montana contributes more than $100 million in economic activities. The incidence of this emerging disease will require changes in conventional disease management.

scholarly journals Characterization of Rhizoctonia spp. Isolates Associated with Damping-Off Disease in Cotton and Tobacco Seedlings in Greece

Plant Disease ◽  
2010 ◽  
Vol 94 (11) ◽  
pp. 1314-1322 ◽  
Author(s):  
C. Bacharis ◽  
A. Gouziotis ◽  
P. Kalogeropoulou ◽  
O. Koutita ◽  
K. Tzavella-Klonari ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Internal Transcribed Spacer ◽  
Damping Off ◽  
Northern Greece ◽  
Internal Transcribed Spacer Region ◽  
Tobacco Plants ◽  
Cotton Plants ◽  
Tobacco Seedlings ◽  
Hyphal Anastomosis

Isolates of Rhizoctonia spp. were obtained during the spring of 2007 from diseased cotton and tobacco seedlings showing damping-off symptoms. The sampled fields were located in the main cotton- and tobacco-cultivating regions of central and northern Greece. Among the 79 isolates obtained from cotton plants, 17 were binucleate and 62 were multinucleate whereas, among the 89 isolates obtained from tobacco plants, 87 were multinucleate and only 2 were binucleate. Characterization of anastomosis groups (AGs) was performed with hyphal anastomosis reactions using tester isolates of known AG groups. Anastomosis reactions in cotton mutlinucleate isolates showed that 54 of them belonged in Rhizoctonia solani AG-4, 6 in AG-7, 1 in AG-2, and 1 in AG-3. In the 87 tobacco multinucleate isolates, anastomosis reactions showed that 70 of them belonged in R. solani AG-2, 16 in AG-4, and 1 in AG-5. In addition, molecular characterization was carried out using the specific ribosomal DNA internal transcribed spacer region, in a randomly selected number of isolates. In cotton, the most prevalent AG was AG-4, with 18 isolates to the subgroup HG-I, 1 isolate to the subgroup HG-II, and 7 isolates to the subgroup HG-III, followed by AG-7 (7 isolates), AG-2-1 (1 isolate), and AG-3 (1 isolate). In tobacco, the most prevalent group was AG-2-1 (70 isolates), followed by AG-4 (6 isolates to the subgroup HG-I and 5 isolates to the subgroup HG-III) and a single isolate belonging to AG-5. Phylogenetic analysis showed that the isolates were distinctly separated based on their AG types. Pathogenicity and aggressiveness of the isolates to several hosts was determined. AG-4 isolates from either cotton or tobacco were the most aggressive on the hosts tested, while AG-2-1 isolates were of moderate aggressiveness and were not pathogenic on barley.

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