scholarly journals Helicobacter delphinicola sp. nov., isolated from common bottlenose dolphins Tursiops truncatus with gastric diseases

2020 ◽  
Vol 141 ◽  
pp. 157-169 ◽  
Author(s):  
T Segawa ◽  
Y Ohno ◽  
S Tsuchida ◽  
K Ushida ◽  
M Yoshioka
Keyword(s):  
16S Rrna ◽  
Tursiops Truncatus ◽  
Cultured Cells ◽  
Bottlenose Dolphins ◽  
Gastric Fluid ◽  
Gastric Ulcers ◽  
Gastric Diseases ◽  
Gastric Fluids ◽  
H Pylori ◽  
Type Strains

Gastritis and gastric ulcers are well-recognized symptoms in cetaceans, and the genus Helicobacter is considered as the main cause. In this study, we examined the gastric fluid of captive common bottlenose dolphins Tursiops truncatus with gastric diseases in order to isolate the organisms responsible for diagnosis and treatment. Four Gram-negative, rod-shaped isolates (TSBT, TSH1, TSZ, and TSH3) with tightly coiled spirals with 2-4 turns and 2-6 bipolar, sheathed flagella, were obtained from gastric fluids of common bottlenose dolphins with gastric diseases. Phylogenetic analysis, based on 16S rRNA, atpA, and 60 kDa heat-shock protein (hsp60) genes, demonstrated that these isolates form a novel lineage within the genus Helicobacter. Analyses of 16S rRNA, atpA, and hsp60 gene sequences showed that isolate TSBT was most closely related to H. cetorum MIT99-5656T (98.5% similarity), H. pylori ATCC 43504T (76.7% similarity), and H. pylori ATCC 43504T (78.0% similarity), respectively. Type strains of Helicobacter showing resistance to 2% NaCl have not been reported previously; however, these novel isolates were resistant to 2% NaCl. Culture supernatant of some isolates induced intracellular vacuolization in mammalian cultured cells. These data, together with the different morphological and biochemical characteristics of the isolates, reveal that these isolates represent a novel species for which we propose the name Helicobacter delphinicola sp. nov. with type strain TSBT (= JCM 32789T = TSD-183T). Future studies will confirm whether H. delphinicola plays a role in lesion etiopathogenesis in cetaceans.

Perfil Epidemiológico de Pacientes Submetidos à Biópsia Gástrica em um Hospital Escola do Sul de Minas Gerais/ Epidemiological Profile of Patients Undergo Gastric Biopsy in a School Hospital of Minas Gerais

1970 ◽  
Vol 4 (3) ◽  
pp. 48-57
Author(s):  
Isabela Maria A. Ribeiro Simões ◽  
Ana Carolina Mauad Coli ◽  
Roseane de Souza Candido Irulegui
Keyword(s):  
Helicobacter Pylori ◽  
Age Groups ◽  
Epidemiological Data ◽  
Minas Gerais ◽  
Gastric Biopsy ◽  
Gastric Ulcers ◽  
Histopathological Diagnosis ◽  
Gastric Diseases ◽  
H Pylori ◽  
Epidemiological Profile

Objetivo: Determinar a prevalência de lesões benignas e neoplasia gástrica através do estudo de biópsias realizadas em um Hospital Escola do Sul de Minas Gerais, no período entre 2007 e 2011. Materiais e Métodos: A pesquisa documental foi quantitativa e retrospectiva, baseada na análise dos registros de biópsias e prontuários. Realizou-se o levantamento de dados referentes à idade, gênero, cor, profissão, diagnóstico histopatológico e presença de Helicobacter pylori nas amostras. Resultados: O número total de biópsias gástricas analisadas foi de 1225, cujo perfil populacional encontrado foi: idade média de 56,75 anos, sexo masculino (52%), cor branca (81,9 %), aposentado (30%). Os diagnósticos mais frequentes foram: gastrites (71,9%), pólipos (14,2%), adenocarcinomas (5,9%), úlceras gástricas (6%), linfomas (0,4%), sem alterações (0,4%) e outros (1,2%). Em outros, encontram-se achados de malignidade, metaplasia e xantelasma gástrico. Em relação à presença de Helicobacter pylori nas amostras, o resultado encontrado foi de24% positivas, 46% negativas e 30% não pesquisadas. Conclusão: Os resultados confirmam a alta frequência das doenças gástricas e sua incidência nas diversas faixas etárias, além do envolvimento do H. pylori em tais afecções. É de grande importância a caracterização dos dados epidemiológicos, o que permite prováveis direcionamentos para programas de prevenção e informação para a população. Palavras-chave: biópsia gástrica, gastropatia, perfil epidemiológico.  ABSTRACTObjective: To determine the prevalence of benign lesions and gastric cancer through study of biopsies performed at a school hospital in southern Minas Gerais, in the period between 2007 and 2011.Materials and Methods: The research was quantitative and retrospective, based on analysis of biopsies records and medical records. We conducted the survey data regarding age, sex, color, profession, histopathological diagnosis and the presence of Helicobacter pylori in the samples. Results: The total number of gastric biopsies analyzed was 1225. Population listing was found: mean age of 56.75 years, male (52%), white (81.9%), retired (30%). The most frequent diagnoses were gastritis (71.9%), polyps (14.2%), adenocarcinomas (5.9%), gastric ulcers (6%), lymphoma (0.4%), unchanged (0, 4%) and others (1.2%). In others, there are: findings of malignancy, metaplasia, gastric xanthelasma. Regarding the presence of Helicobacter pylori in the sample, the result was: 24% positive, 46% negative, 30% non searched. Conclusion: The results confirm the high frequency of gastric diseases and their incidence in the various age groups additionally to the involvement of H. pylori in such conditions. It is of great importance to characterize the epidemiological data, allowing probable directions for prevention and information programs for population. Keywords: gastric biopsy, gastropathy, epidemiological profile

scholarly journals PCR assay targeting virulence genes of Helicobacter pylori isolated from drinking water and clinical samples in Lahore metropolitan, Pakistan

2011 ◽  
Vol 9 (1) ◽  
pp. 208-216 ◽  
Author(s):  
Zahoor Qadir Samra ◽  
Umber Javaid ◽  
Sadia Ghafoor ◽  
Aleeza Batool ◽  
Nadia Dar ◽  
...  
Keyword(s):  
Drinking Water ◽  
16S Rrna ◽  
Virulence Genes ◽  
Public Health Education ◽  
Gastric Ulcers ◽  
Clinical Samples ◽  
Gastric Disease ◽  
Lower Socioeconomic ◽  
H Pylori ◽  
Species Specific

Helicobacter pylorus is considered for chronic gastritis, gastric ulcers and adenocarcinoma and its high infection rate is observed in overcrowded and lower socioeconomic groups in developing countries. This study was designed to identify the role of drinking water in the transmission and prevalence of H. pylori (HP). Selective HP medium was developed for enrichment and presumptive identification of H. pylori by urease, catalase and species specific 16S rRNA tests. The virulence genes (vacA ‘s’ and ‘m’ regions and cagA) of H. pylori in 90 out of 225 H. pylori positive drinking water samples were present (40%). Ten out of 18 biopsies (55.55%) and 15 out of 50 vomiting fluids of gastric disease patients (30%) were also positive for virulence genes. Anti-H. pylori antibodies were also detected in 31 out of 50 patients’ sera. The presence of virulence genes was also directly confirmed by hybridization studies using non-radioactive DNA probes of 16S rRNA, vacA and cagA genes. The presence of H. pylori in water is due to poor sanitary conditions, improper waste disposal and lack of public health education. PCR-based analysis and colony hybridization can be used for detection of H. pylori in clinical and environmental samples.

scholarly journals In Vitro Screening Potential Antibacterial Properties of the Greek Oregano Honey against Clinical Isolates of Helicobacter pylori

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1568
Author(s):  
Chrysoula (Chrysa) Voidarou ◽  
Georgios Rozos ◽  
Athanasios Alexopoulos ◽  
Stavros Plessas ◽  
Ioanna Mantzourani ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibacterial Properties ◽  
Gastric Ulcers ◽  
Mountainous Area ◽  
Bacterial Strains ◽  
Gastric Diseases ◽  
Popular Medicine ◽  
Traditional Remedy ◽  
H Pylori

Oregano honey is an exceedingly rare and distinct product, not commercially available, produced by bees bred in oregano fields of alpine altitudes at the mountainous area of Epirus, Greece. In ethnic popular medicine, this product is used as a therapeutic in various gastric diseases. To test this hypothesis, 14 strains of Helicobacter pylori (H. pylori), 6 isolated from gastric ulcers and 8 from cases of clinical gastritis, were employed in the present study. The above bacterial strains were exposed to various concentrations (75% v/v, 50% v/v, 25% v/v, 12.5% v/v, and 6% v/v) of 50 oregano honey samples by using the agar well method and the inhibition zones observed around each well were recorded. Although the inhibitory zones of the H. pylori isolated from the gastric ulcers were wide enough (0–34 mm), those strains, in general, appeared more resistant than the other eight (0–58 mm). The same result was observed when the same strains were tested against six antibiotics used in clinical practice. Extracts of oregano honey were prepared by extraction with four different organic solvents. N-hexane and chloroform extracts had the most potent antibacterial action. Finally, pure oregano honey and diethyl ether extracts of honey showed significant inhibitory activity against urease secreted by the pathogen. These results strongly indicate the susceptibility of H. pylori strains to the oregano honey by more than one mode of action. Consequently, this variety of honey seems to have potential therapeutic properties against gastric ulcers and gastritis, thus explaining the preference of the locals towards this traditional remedy.

Seasonal Abundance and Site Fidelity of Bottlenose Dolphins (Tursiops truncatus) in Mississippi Sound

2004 ◽  
Vol 30 (2) ◽  
pp. 299-310 ◽  
Author(s):  
Carrie W. Hubard ◽  
Kathy Maze-Foley ◽  
Keith D. Mullin ◽  
William W. Schroeder
Keyword(s):  
Tursiops Truncatus ◽  
Site Fidelity ◽  
Bottlenose Dolphins ◽  
Seasonal Abundance ◽  
Mississippi Sound

Whale Poaching Detection Based on Microscopic Characteristics of Bottlenose Dolphins’ (Tursiops truncatus) Bone Fragments

2018 ◽  
Vol 43 (5) ◽  
pp. 519-528
Author(s):  
Manuela Zadravec ◽  
Zvonimir Kozarić ◽  
Snježana Kužir ◽  
Mario Mitak ◽  
Tomislav Gomerčić ◽  
...  
Keyword(s):  
Tursiops Truncatus ◽  
Bottlenose Dolphins ◽  
Bone Fragments

Preliminary Analysis of the Proteome of Exhaled Breath Condensate in Bottlenose Dolphins (Tursiops truncatus)

2018 ◽  
Vol 44 (3) ◽  
pp. 256-266 ◽  
Author(s):  
Don R. Bergfelt ◽  
John Lippolis ◽  
Michel Vandenplas ◽  
Sydney Davis ◽  
Blake A. Miller ◽  
...  
Keyword(s):  
Preliminary Analysis ◽  
Tursiops Truncatus ◽  
Exhaled Breath Condensate ◽  
Bottlenose Dolphins ◽  
Exhaled Breath ◽  
Breath Condensate

Bottlenose Dolphins (Tursiops truncatus) Increase Number of Whistles When Feeding

2004 ◽  
Vol 30 (3) ◽  
pp. 357-362 ◽  
Author(s):  
Alejandro Acevedo-Gutiérrez ◽  
Sarah C. Stienessen
Keyword(s):  
Tursiops Truncatus ◽  
Bottlenose Dolphins

Spatiotemporal Movements of Common Bottlenose Dolphins (Tursiops truncatus truncatus) in Northeast Florida, USA

2020 ◽  
Vol 46 (3) ◽  
pp. 285-300 ◽  
Author(s):  
Marilyn Mazzoil ◽  
Quincy Gibson ◽  
Wendy Noke Durden ◽  
Rose Borkowski ◽  
George Biedenbach ◽  
...  
Keyword(s):  
Tursiops Truncatus ◽  
Bottlenose Dolphins
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