scholarly journals PCR assay targeting virulence genes of Helicobacter pylori isolated from drinking water and clinical samples in Lahore metropolitan, Pakistan

2011 ◽  
Vol 9 (1) ◽  
pp. 208-216 ◽  
Author(s):  
Zahoor Qadir Samra ◽  
Umber Javaid ◽  
Sadia Ghafoor ◽  
Aleeza Batool ◽  
Nadia Dar ◽  
...  
Keyword(s):  
Drinking Water ◽  
16S Rrna ◽  
Virulence Genes ◽  
Public Health Education ◽  
Gastric Ulcers ◽  
Clinical Samples ◽  
Gastric Disease ◽  
Lower Socioeconomic ◽  
H Pylori ◽  
Species Specific

Helicobacter pylorus is considered for chronic gastritis, gastric ulcers and adenocarcinoma and its high infection rate is observed in overcrowded and lower socioeconomic groups in developing countries. This study was designed to identify the role of drinking water in the transmission and prevalence of H. pylori (HP). Selective HP medium was developed for enrichment and presumptive identification of H. pylori by urease, catalase and species specific 16S rRNA tests. The virulence genes (vacA ‘s’ and ‘m’ regions and cagA) of H. pylori in 90 out of 225 H. pylori positive drinking water samples were present (40%). Ten out of 18 biopsies (55.55%) and 15 out of 50 vomiting fluids of gastric disease patients (30%) were also positive for virulence genes. Anti-H. pylori antibodies were also detected in 31 out of 50 patients’ sera. The presence of virulence genes was also directly confirmed by hybridization studies using non-radioactive DNA probes of 16S rRNA, vacA and cagA genes. The presence of H. pylori in water is due to poor sanitary conditions, improper waste disposal and lack of public health education. PCR-based analysis and colony hybridization can be used for detection of H. pylori in clinical and environmental samples.

scholarly journals Frequency of endoscopic findings of equine squamous gastric disease (ESGD) in rural horses and its association with Helicobacter pylori virulence genes

2021 ◽  
Author(s):  
F Rezazadeh ◽  
N Pourebrahimi ◽  
R Ghotaslou ◽  
M Golshani Nasab ◽  
MY Memar
Keyword(s):  
Helicobacter Pylori ◽  
Virulence Genes ◽  
Gastric Fluid ◽  
Gastric Disease ◽  
Specific Gene ◽  
Squamous Mucosa ◽  
Faecal Samples ◽  
Potential Risk Factors ◽  
H Pylori ◽  
Lesion Severity

Equine gastric ulcer syndrome (EGUS) is a multifactorial disorder and one of the most common diseases in horses. The objective of this research was to detect one of the potential risk factors of equine squamous gastric disease (ESGD), the Helicobacter pylori specific gene, and tracing the presence of the duodenal ulcer-promoting gene (dupA) as a possible virulence marker. Gastric fluid together with faecal samples were collected from twenty rural horses from around Tabriz, Iran. Throughout the endoscopic examinations, the type, numbers, severity, and the location of the lesions were documented. Nine of twenty horses exhibited macroscopic lesions in the squamous mucosa that were later classified into grades 1, 2, 3, and 4. Only three of these horses exhibited H. pylori in their gastric fluid samples, whereas all faecal samples were H. pylori-negative. All the H. pylori-positive cases manifested severe forms of ESGD (grades 3–4). The age and sex were both unrelated to the lesion severity and ESGD status in this study. Research is required to further discuss the virulence aspects of dupA regarding ESGD.

scholarly journals Semiquantitative Species-Specific Detection of Bartonella henselae and Bartonella quintana by PCR-Enzyme Immunoassay

1999 ◽  
Vol 37 (10) ◽  
pp. 3097-3101 ◽  
Author(s):  
Anna Sander ◽  
Susanne Penno
Keyword(s):  
16S Rrna ◽  
Enzyme Immunoassay ◽  
Bartonella Henselae ◽  
Clinical Samples ◽  
Species Differentiation ◽  
Target Sequence ◽  
Simple Method ◽  
Bartonella Quintana ◽  
Pcr Products ◽  
Species Specific

Bartonella henselae is the main causative agent of cat-scratch disease, and both B. henselae andBartonella quintana cause angioproliferative disorders such as bacillary angiomatosis. To increase the sensitivity ofBartonella detection by PCR and to improve the species differentiation, we developed a semiquantitative, species-specific PCR-based enzyme immunoassay (EIA). The 16S rRNA gene was selected as the target sequence. Internal nucleotide sequences derived from the amplified 16S rRNA region were used to develop species-specific oligonucleotide probes for B. henselae and B. quintana. Biotin-labeled PCR products were immobilized on streptavidin-coated microtiter plates, hybridized to a digoxigenin-labeled probe, and detected with antidigoxigenin peroxidase conjugate. No cross-hybridization with other Bartonella or non-Bartonella species was observed. This EIA was as sensitive as dot blot hybridization and was 10 times more sensitive than visualization of PCR products on agarose gels. Serial dilutions ofB. henselae and B. quintana suspensions demonstrated that an optical density (OD) of approximately 0.200 was equivalent to 5 CFU in the reaction mixture. By comparing the OD of the bacterial dilutions with that obtained from clinical specimens we could determine that the number of CFU in clinical samples ranged from 103 to 106 CFU/ml. The PCR-EIA developed in the present study is a rapid, sensitive, and simple method for the diagnosis of B. henselae and B. quintanainfections.

scholarly journals In Vivo Analysis of the Viable Microbiota and Helicobacter pylori Transcriptome in Gastric Infection and Early Stages of Carcinogenesis

2017 ◽  
Vol 85 (10) ◽  
Author(s):  
Kaisa Thorell ◽  
Johan Bengtsson-Palme ◽  
Oscar Hsin-Fu Liu ◽  
Reyna Victoria Palacios Gonzales ◽  
Intawat Nookaew ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Disease Progression ◽  
Ph Regulation ◽  
Gastric Ulcers ◽  
Gastric Disease ◽  
Content Type ◽  
Expression Of Genes ◽  
H Pylori

ABSTRACT Emerging evidence shows that the human microbiota plays a larger role in disease progression and health than previously anticipated. Helicobacter pylori, the causative agent of gastric cancer and duodenal and gastric ulcers, was early associated with gastric disease, but it has also been proposed that the accompanying microbiota in Helicobacter pylori-infected individuals might affect disease progression and gastric cancer development. In this study, the composition of the transcriptionally active microbial community and H. pylori gene expression were determined using metatranscriptomic RNA sequencing of stomach biopsy specimens from individuals with different H. pylori infection statuses and premalignant tissue changes. The results show that H. pylori completely dominates the microbiota not only in infected individuals but also in most individuals classified as H. pylori uninfected using conventional methods. Furthermore, H. pylori abundance is positively correlated with the presence of Campylobacter, Deinococcus, and Sulfurospirillum. Finally, we quantified the expression of a large number of Helicobacter pylori genes and found high expression of genes involved in pH regulation and nickel transport. Our study is the first to dissect the viable microbiota of the human stomach by metatranscriptomic analysis, and it shows that metatranscriptomic analysis of the gastric microbiota is feasible and can provide new insights into how bacteria respond in vivo to variations in the stomach microenvironment and at different stages of disease progression.

scholarly journals Helicobacter delphinicola sp. nov., isolated from common bottlenose dolphins Tursiops truncatus with gastric diseases

2020 ◽  
Vol 141 ◽  
pp. 157-169 ◽  
Author(s):  
T Segawa ◽  
Y Ohno ◽  
S Tsuchida ◽  
K Ushida ◽  
M Yoshioka
Keyword(s):  
16S Rrna ◽  
Tursiops Truncatus ◽  
Cultured Cells ◽  
Bottlenose Dolphins ◽  
Gastric Fluid ◽  
Gastric Ulcers ◽  
Gastric Diseases ◽  
Gastric Fluids ◽  
H Pylori ◽  
Type Strains

Gastritis and gastric ulcers are well-recognized symptoms in cetaceans, and the genus Helicobacter is considered as the main cause. In this study, we examined the gastric fluid of captive common bottlenose dolphins Tursiops truncatus with gastric diseases in order to isolate the organisms responsible for diagnosis and treatment. Four Gram-negative, rod-shaped isolates (TSBT, TSH1, TSZ, and TSH3) with tightly coiled spirals with 2-4 turns and 2-6 bipolar, sheathed flagella, were obtained from gastric fluids of common bottlenose dolphins with gastric diseases. Phylogenetic analysis, based on 16S rRNA, atpA, and 60 kDa heat-shock protein (hsp60) genes, demonstrated that these isolates form a novel lineage within the genus Helicobacter. Analyses of 16S rRNA, atpA, and hsp60 gene sequences showed that isolate TSBT was most closely related to H. cetorum MIT99-5656T (98.5% similarity), H. pylori ATCC 43504T (76.7% similarity), and H. pylori ATCC 43504T (78.0% similarity), respectively. Type strains of Helicobacter showing resistance to 2% NaCl have not been reported previously; however, these novel isolates were resistant to 2% NaCl. Culture supernatant of some isolates induced intracellular vacuolization in mammalian cultured cells. These data, together with the different morphological and biochemical characteristics of the isolates, reveal that these isolates represent a novel species for which we propose the name Helicobacter delphinicola sp. nov. with type strain TSBT (= JCM 32789T = TSD-183T). Future studies will confirm whether H. delphinicola plays a role in lesion etiopathogenesis in cetaceans.

scholarly journals Searching for Helicobacter pylori and Chlamydia pneumoniae in primary endodontic infections

2012 ◽  
Vol 06 (02) ◽  
pp. 158-162 ◽  
Author(s):  
Isabela N Rôças ◽  
José F Siqueira
Keyword(s):  
Helicobacter Pylori ◽  
Chlamydia Pneumoniae ◽  
Multiple Displacement Amplification ◽  
Clinical Sample ◽  
Clinical Samples ◽  
Human Pathogens ◽  
Root Canals ◽  
Endodontic Infections ◽  
H Pylori ◽  
Species Specific

ABSTRACTObjectives: The purpose of this study was to search samples from primary endodontic infections for the presence of two common human bacterial pathogens -Helicobacter pylori and Chlamydia pneumoniaeMethods: Genomic DNA isolated from samples taken from 25 root canals of teeth with asymptomatic (chronic) apical periodontitis and 25 aspirates from acute apical abscess was initially amplified by the multiple displacement amplification approach and then used as template in species-specific polymerase chain reaction (PCR) for detection of H. pylori and C. pneumoniae. Results: All clinical samples were positive for the presence of bacterial DNA. However, no clinical sample was positive for either H. pylori or C. pneumoniaeConclusions: Neither H. pylori nor C. pneumoniae were found in samples from primary endodontic infections. These findings suggest that these species are not candidate endodontic pathogens and that the necrotic root canal does not serve as a reservoir for these human pathogens in healthy patients. (Eur J Dent 2012;6:158-162)

Perfil Epidemiológico de Pacientes Submetidos à Biópsia Gástrica em um Hospital Escola do Sul de Minas Gerais/ Epidemiological Profile of Patients Undergo Gastric Biopsy in a School Hospital of Minas Gerais

1970 ◽  
Vol 4 (3) ◽  
pp. 48-57
Author(s):  
Isabela Maria A. Ribeiro Simões ◽  
Ana Carolina Mauad Coli ◽  
Roseane de Souza Candido Irulegui
Keyword(s):  
Helicobacter Pylori ◽  
Age Groups ◽  
Epidemiological Data ◽  
Minas Gerais ◽  
Gastric Biopsy ◽  
Gastric Ulcers ◽  
Histopathological Diagnosis ◽  
Gastric Diseases ◽  
H Pylori ◽  
Epidemiological Profile

Objetivo: Determinar a prevalência de lesões benignas e neoplasia gástrica através do estudo de biópsias realizadas em um Hospital Escola do Sul de Minas Gerais, no período entre 2007 e 2011. Materiais e Métodos: A pesquisa documental foi quantitativa e retrospectiva, baseada na análise dos registros de biópsias e prontuários. Realizou-se o levantamento de dados referentes à idade, gênero, cor, profissão, diagnóstico histopatológico e presença de Helicobacter pylori nas amostras. Resultados: O número total de biópsias gástricas analisadas foi de 1225, cujo perfil populacional encontrado foi: idade média de 56,75 anos, sexo masculino (52%), cor branca (81,9 %), aposentado (30%). Os diagnósticos mais frequentes foram: gastrites (71,9%), pólipos (14,2%), adenocarcinomas (5,9%), úlceras gástricas (6%), linfomas (0,4%), sem alterações (0,4%) e outros (1,2%). Em outros, encontram-se achados de malignidade, metaplasia e xantelasma gástrico. Em relação à presença de Helicobacter pylori nas amostras, o resultado encontrado foi de24% positivas, 46% negativas e 30% não pesquisadas. Conclusão: Os resultados confirmam a alta frequência das doenças gástricas e sua incidência nas diversas faixas etárias, além do envolvimento do H. pylori em tais afecções. É de grande importância a caracterização dos dados epidemiológicos, o que permite prováveis direcionamentos para programas de prevenção e informação para a população. Palavras-chave: biópsia gástrica, gastropatia, perfil epidemiológico.  ABSTRACTObjective: To determine the prevalence of benign lesions and gastric cancer through study of biopsies performed at a school hospital in southern Minas Gerais, in the period between 2007 and 2011.Materials and Methods: The research was quantitative and retrospective, based on analysis of biopsies records and medical records. We conducted the survey data regarding age, sex, color, profession, histopathological diagnosis and the presence of Helicobacter pylori in the samples. Results: The total number of gastric biopsies analyzed was 1225. Population listing was found: mean age of 56.75 years, male (52%), white (81.9%), retired (30%). The most frequent diagnoses were gastritis (71.9%), polyps (14.2%), adenocarcinomas (5.9%), gastric ulcers (6%), lymphoma (0.4%), unchanged (0, 4%) and others (1.2%). In others, there are: findings of malignancy, metaplasia, gastric xanthelasma. Regarding the presence of Helicobacter pylori in the sample, the result was: 24% positive, 46% negative, 30% non searched. Conclusion: The results confirm the high frequency of gastric diseases and their incidence in the various age groups additionally to the involvement of H. pylori in such conditions. It is of great importance to characterize the epidemiological data, allowing probable directions for prevention and information programs for population. Keywords: gastric biopsy, gastropathy, epidemiological profile

scholarly journals Intracellular Presence of Helicobacter pylori and Its Virulence-Associated Genotypes within the Vaginal Yeast of Term Pregnant Women

2021 ◽  
Vol 9 (1) ◽  
pp. 131
Author(s):  
Kimberly Sánchez-Alonzo ◽  
Lillian Matamala-Valdés ◽  
Cristian Parra-Sepúlveda ◽  
Humberto Bernasconi ◽  
Víctor L. Campos ◽  
...  
Keyword(s):  
Risk Factors ◽  
Helicobacter Pylori ◽  
Virulence Genes ◽  
Yeast Cells ◽  
Bacterial Identification ◽  
Infection Risk Factors ◽  
Horse Blood ◽  
Total Dna ◽  
H Pylori ◽  
Total Yeast

Background: Helicobacter pylori transmission routes are not entirely elucidated. Since yeasts are postulated to transmit this pathogen, this study aimed to detect and genotype intracellular H. pylori harbored within vaginal yeast cells. Methods: A questionnaire was used to determine risk factors of H. pylori infection. Samples were seeded on Sabouraud Dextrose Agar and horse blood-supplemented Columbia agar. Isolated yeasts were identified using and observed by optical microscopy searching for intra-yeast H. pylori. Total yeast DNA, from one random sample, was extracted to search for H. pylori virulence genes by PCR and bacterial identification by sequencing. Results: 43% of samples contained yeasts, mainly Candida albicans (91%). Microscopy detected bacteria such as bodies and anti-H. pylori antibodies binding particles in 50% of the isolated yeasts. Total DNA extracted showed that 50% of the isolated yeasts were positive for H. pylori 16S rDNA and the sequence showed 99.8% similarity with H. pylori. In total, 32% of H. pylori DNA positive samples were cagA+ vacAs1a vacAm1 dupA−. No relationship was observed between possible H. pylori infection risk factors and vaginal yeasts harboring this bacterium. Conclusion: H. pylori having virulent genotypes were detected within vaginal yeasts constituting a risk for vertical transmission of this pathogen.

scholarly journals A Comparative Analysis of the Government and NGOs in Delivering Quality Services for the Rural People of Pakistan: Community Perspectives

2020 ◽  
Vol 30 (1-2) ◽  
pp. 203-225
Author(s):  
Mohsin Khan ◽  
Jetnor Kasmi ◽  
Abdul Saboor ◽  
Iftikhar Ali
Keyword(s):  
Drinking Water ◽  
Service Delivery ◽  
Rural Areas ◽  
Public Health Education ◽  
Road Transport ◽  
Extension Services ◽  
Rural People ◽  
Basic Services ◽  
The Government ◽  
The Impact

Often the government and the non-governmental organisations (NGOs) are criticised for their poor performances in delivering services particularly in rural areas. However, there has been limited research on the assessment of their relative performances in service delivery as well as on the perceptions of people on the quality of such service delivery. This study examines the relative performances of NGOs and the governmental development interventions that provide basic services including public health, education, drinking water and sanitation. The study explains the impact of agricultural extension services and infrastructure such as access to roads and markets on the rural people and measures the satisfaction level of the rural community. For this purpose, 225 households (HHs) in 8 villages of Phalia Tehsil, district Mandi Bahauddin, Punjab, Pakistan were first surveyed in 2010 and then in 2014 using a structured questionnaire. The findings reveal different satisfaction levels of HHs, with most of them expressing less satisfaction on government service delivery compared with NGOs. They reveal satisfaction over the performance of NGOs in health, drinking water supplies and agriculture extension services. Further, the study shows an increasing satisfaction of people on access to road, transport, agri-market and price of agri-commodities by the government.

scholarly journals Nocardioides hungaricus sp. nov., isolated from a drinking water supply system

2011 ◽  
Vol 61 (3) ◽  
pp. 549-553 ◽  
Author(s):  
Erika M. Tóth ◽  
Zsuzsa Kéki ◽  
Judit Makk ◽  
Zalán G. Homonnay ◽  
Károly Márialigeti ◽  
...  
Keyword(s):  
Drinking Water ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Water Supply ◽  
Gene Sequence ◽  
Supply System ◽  
Drinking Water Supply ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Drinking Water Supply System

Three Gram-positive, rod-shaped bacterial strains were isolated from the drinking water supply system of the Hungarian capital, Budapest. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparison revealed that the isolates represented a distinct cluster within the clade of the genus Nocardioides and were most closely related to Nocardioides pyridinolyticus OS4T, Nocardioides aquiterrae GW-9T, Nocardioides sediminis MSL-01T and N. hankookensis DS-30T. The peptidoglycan based on ll-2,6-diaminopimelic acid, the major menaquinone MK-8(H4), the cellular fatty acid profile with iso-C16 : 0 and anteiso-C17 : 0 as predominating components and the DNA G+C content of 71.4 mol% (strain 1RaM5-12T) were consistent with the affiliation of the isolates to the genus Nocardioides. Because of differences in physiological characteristics, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of protein extracts, PvuII RiboPrinter patterns and 96.1 % 16S rRNA gene sequence similarity between strain 1RaM5-12T and its closest phylogenetic neighbour, N. pyridinolyticus OS4T, a novel species, Nocardioides hungaricus sp. nov., is proposed. The type strain is 1RaM5-12T (=DSM 21673T =NCAIM 02330T).

scholarly journals Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant

2012 ◽  
Vol 62 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Ivone Vaz-Moreira ◽  
Vânia Figueira ◽  
Ana R. Lopes ◽  
Alexandre Lobo-da-Cunha ◽  
Cathrin Spröer ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Rrna Gene ◽  
Rrna Gene Sequence

A Gram-positive, aerobic, non-motile, endospore-forming rod, designated DS22T, was isolated from a drinking-water treatment plant. Cells were catalase- and oxidase-positive. Growth occurred at 15–37 °C, at pH 7–10 and with <8 % (w/v) NaCl (optimum growth: 30 °C, pH 7–8 and 1–3 % NaCl). The major respiratory quinone was menaquinone 7, the G+C content of the genomic DNA was 36.5 mol% and the cell wall contained meso-diaminopimelic acid. On the basis of 16S rRNA gene sequence analysis, strain DS22T was a member of the genus Bacillus. Its closest phylogenetic neighbours were Bacillus horneckiae NRRL B-59162T (98.5 % 16S rRNA gene sequence similarity), Bacillus oceanisediminis H2T (97.9 %), Bacillus infantis SMC 4352-1T (97.4 %), Bacillus firmus IAM 12464T (96.8 %) and Bacillus muralis LMG 20238T (96.8 %). DNA–DNA hybridization, and biochemical and physiological characterization allowed the differentiation of strain DS22T from its closest phylogenetic neighbours. The data supports the proposal of a novel species, Bacillus purgationiresistans sp. nov.; the type strain is DS22T ( = DSM 23494T = NRRL B-59432T = LMG 25783T).

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