Spontaneous adenocarcinoma of the gas gland in Nothobranchius fishes

2020 ◽  
Vol 137 (3) ◽  
pp. 205-210
Author(s):  
I Dyková ◽  
R Blažek ◽  
K Soucˇková ◽  
M Reichard ◽  
O Slabý
Keyword(s):  
Gas Gland

Correlation Between PTEN and VEGF Expressions During the Development of Gas Gland in Japanese Eel (Anguilla japonica) Stimulated by Exogenous Hypophyseal Factors

2010 ◽  
Vol 1 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Yi-Fan Chen ◽  
Shan-Ru Jeng ◽  
Ming-Chyuan Chen ◽  
Jin-Chywan Gwo ◽  
Yung-Sen Huang
Keyword(s):  
Anguilla Japonica ◽  
Japanese Eel ◽  
Gas Gland

scholarly journals The Active Transport of Oxygen and Carbon Dioxide into the Swim-Bladder of Fish

1966 ◽  
Vol 49 (6) ◽  
pp. 1209-1220 ◽  
Author(s):  
H.J. KUHN ◽  
E. MARTI
Keyword(s):  
Carbon Dioxide ◽  
Lactic Acid ◽  
Active Transport ◽  
Bladder Pressure ◽  
Swim Bladder ◽  
Rete Mirabile ◽  
Partial Pressures ◽  
Gas Gland ◽  
Counter Current ◽  
Good Agreement

The active transport of oxygen and carbon dioxide into the swim-bladder of fish is discussed. The rete mirabile is a capillary network which is involved in the gas secretion into the bladder. The rete is regarded as a counter-current multiplier. Lactic acid which is produced in the gas gland generates in the rete single concentrating effects for oxygen and carbon dioxide; i.e., for equal partial pressures the concentrations of the gases in the afferent rete capillaries are higher than those in the efferent ones. The single concentrating effects were calculated from measurements of sea robin blood (Root, 1931). The multiplication of these effects within the rete for different rete lengths and different transport rates was numerically evaluated. The calculated O2 and CO2 pressures in the bladder are in good agreement with the experimental results of Scholander and van Dam (1953). The descent velocities at equilibrium between bladder pressure and hydrostatic pressure are discussed for fishes with different rete lengths.

Mechanisms of acid release in isolated gas gland cells of the European eel Anguilla anguilla

1995 ◽  
Vol 269 (4) ◽  
pp. R793-R799 ◽  
Author(s):  
B. Pelster
Keyword(s):  
Carbonic Anhydrase ◽  
Disulfonic Acid ◽  
European Eel ◽  
Proton Release ◽  
Isolated Cells ◽  
Extracellular Medium ◽  
Proton Secretion ◽  
Gland Cells ◽  
Acid Release ◽  
Gas Gland

Mechanisms of acid production and of acid release have been analyzed in isolated gas gland cells of the eel swimbladder using a cytosensor microphysiometer. Incubation of isolated cells with oxamic acid caused a dose-dependent decrease in the rate of proton release. At the highest oxamic acid concentration used (20 mmol/l), proton release was reduced by approximately 40%; incubation with sodium fluoride (10 mmol/l) or removal of glucose from the extracellular medium caused 60 and 80% reduction, respectively. NaCN had little effect on proton secretion. Proton release of isolated gas gland cells was largely dependent on the extracellular sodium concentration, and this sodium effect was in part inhibitable by amiloride. A 15-20% reduction in the rate of proton secretion was observed in the presence of 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid, an inhibitor of anion exchange. Inhibition of mammalian H(+)-K(+)-adenosinetriphosphatase with omeprazole had no effect, whereas bafilomycin, an inhibitor of vesicular H(+)-adenosinetriphosphatase, induced a 25% reduction in proton secretion. Ethoxzolamide, a membrane-permeable inhibitor of carbonic anhydrase, caused a 60% reduction in proton secretion (inhibition constant = 54.4 nmol/l). Prontosil-dextran, a membrane-impermeable sulfonamide, also reduced the proton release, thus indicating the presence of a membrane-bound carbonic anhydrase facing the extracellular space.

Control of acid secretion in cultured gas gland cells of the European eel Anguilla anguilla

1996 ◽  
Vol 270 (3) ◽  
pp. R578-R584
Author(s):  
B. Pelster ◽  
L. Pott
Keyword(s):  
Acid Secretion ◽  
Primary Culture ◽  
Single Cells ◽  
Calf Serum ◽  
Anguilla Anguilla ◽  
Beta Agonist ◽  
European Eel ◽  
Adrenergic Stimulation ◽  
Gland Cells ◽  
Gas Gland

Single cells and cell clusters isolated from the swimbladder epithelium of the European eel Anguilla anguilla attached to collagen S-coated petri dishes and proliferated in a modified Dulbecco's modified Eagle's medium, supplemented with 0.5% fetal calf serum. At a temperature of 20-22 degrees C, the growing colonies reached confluence typically within 6-8 days. Activities of glycolytic and pentose phosphate shunt enzymes remained stable or increased only slightly during the first 10 days of primary culture. Incubated in a defined medium providing glucose as a fuel, gas gland cells in primary culture produced and released lactic acid. The rate of acid secretion of cultured gas gland cells measured with a cytosensor microphysiometer was not influenced by cholinergic stimulation. Similarly, the Ca2+ ionophore A-23187 had no effect. Adrenergic stimulation with epinephrine or the beta-agonist isoproterenol also did not increase the rate of acid secretion, indicating that in gas gland cells the metabolic activity cannot be stimulated via beta-adrenergic stimulation followed by an increase in adenosine 3',5'-cyclic monophosphate (cAMP). Artificially increasing the intracellular concentration of cAMP by incubation with forskolin or the cAMP analogue 8-(4-chlorophenylthio)-cAMP even resulted in a marked reduction in the rate of acid secretion. The results demonstrate that primary cell culture provides a useful means for the analysis of metabolic control and of ion transfer processes in swimbladder gas gland cells.

scholarly journals THE FORMATION OF MULTIVESICULAR BODIES FROM THE NUCLEAR ENVELOPE

1970 ◽  
Vol 45 (2) ◽  
pp. 205-211 ◽  
Author(s):  
Wincenty Kilarski ◽  
Andrzej Jasiński
Keyword(s):  
Electron Microscope ◽  
Nuclear Envelope ◽  
Perca Fluviatilis ◽  
Multivesicular Bodies ◽  
Swim Bladder ◽  
Perch Perca Fluviatilis ◽  
Mechanisms Of Formation ◽  
Gas Gland ◽  
Intense Activity ◽  
Active Proliferation

Cells of the gas gland of the perch Perca fluviatilis L., stimulated to increased generation of gas by the repeated emptying of the swim-bladder, were examined in the electron microscope. Intense activity of the nuclear envelope was demonstrated. Simple vesicles originating from the external nuclear membrane and the so-called multivesicular bodies derived from the outpocketings of both membranes of the nuclear envelope were observed. The multivesicular bodies were filled with numerous fine vesiculae arising from the active proliferation of their internal membrane. The authors offer two alternative mechanisms of formation of fine vesiculae inside the multivesicular bodies and the mechanism of the tearing away of these bodies from the nuclear envelope.

scholarly journals Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0239627
Author(s):  
Gabriel Schneebauer ◽  
Constantin Lindemann ◽  
Victoria Drechsel ◽  
Lasse Marohn ◽  
Klaus Wysujack ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
European Eel ◽  
Gland Cells ◽  
Elevated Hydrostatic Pressure ◽  
Glycolytic Activity ◽  
Gas Gland
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