scholarly journals Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. I. Initial comparison of four protocols

2014 ◽  
Vol 111 (1) ◽  
pp. 1-13 ◽  
Author(s):  
JV Warg ◽  
T Clement ◽  
ER Cornwell ◽  
A Cruz ◽  
RG Getchell ◽  
...  
Keyword(s):  
Real Time ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Rt Pcr ◽  
Viral Hemorrhagic Septicemia ◽  
Initial Comparison

scholarly journals Molecular Epidemiology of Novirhabdoviruses Emerging in Iranian Trout Farms

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 448
Author(s):  
Sohrab Ahmadivand ◽  
Dušan Palić ◽  
Manfred Weidmann
Keyword(s):  
Amino Acids ◽  
Haplotype Analysis ◽  
Economic Losses ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Rt Pcr ◽  
Necrosis Virus ◽  
Viral Hemorrhagic Septicemia ◽  
Pcr Assays ◽  
Clade 1 ◽  
Trout Farms

Novirhabdoviruses cause large epizootics and economic losses of farmed trout. In this study, we surveyed Viral hemorrhagic septicemia virus and Infectious hematopoietic and necrosis virus (VHSV and IHNV) through both monitoring and investigation of clinical outbreaks reported by farmers in the regions with major rainbow trout production in Iran from 2015 to 2019. RT-PCR assays of the kidney samples and cell culture (EPC/FHM cells) samples confirmed the presence of the viruses, with 9 VHSV and 4 IHNV isolates, in both endemic and new areas of Iran. Sequence analysis of the G gene revealed that VHSV isolates belonged to genogroup Ia, and IHNV isolates were clustered into genogroup E, both typical for isolates from European countries. A haplotype analysis based on non-homologous amino acids of the G gene supports the emergence of two lineages of IHNV from clade 1 (E-1), as well as VHSV clade 2 (Ia-2) of the European genogroups, confirming that VHSV and IHNV isolates in Iran, have originated from Europe possibly via imported eggs.

Comparison of Quantitative RT-PCR with Cell Culture to Detect Viral Hemorrhagic Septicemia Virus (VHSV) IVb Infections in the Great Lakes

2010 ◽  
Vol 22 (1) ◽  
pp. 50-61 ◽  
Author(s):  
Kristine M. Hope ◽  
Rufina N. Casey ◽  
Geoffrey H. Groocock ◽  
Rodman G. Getchell ◽  
Paul R. Bowser ◽  
...  
Keyword(s):  
Cell Culture ◽  
Great Lakes ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Rt Pcr ◽  
Viral Hemorrhagic Septicemia

scholarly journals Accurate Detection and Quantification of the Fish Viral Hemorrhagic Septicemia virus (VHSv) with a Two-Color Fluorometric Real-Time PCR Assay

PLoS ONE ◽  
2013 ◽  
Vol 8 (8) ◽  
pp. e71851 ◽  
Author(s):  
Lindsey R. Pierce ◽  
James C. Willey ◽  
Vrushalee V. Palsule ◽  
Jiyoun Yeo ◽  
Brian S. Shepherd ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Pcr Assay ◽  
Viral Hemorrhagic Septicemia ◽  
Accurate Detection ◽  
Detection And Quantification

531: Quantitative Real-Time RT-PCR for AMACR Distinguishes Prostate Cancer with High Specificity From Benign Lesions

2005 ◽  
Vol 173 (4S) ◽  
pp. 145-145 ◽  
Author(s):  
Martin Schostak ◽  
Hans Krause ◽  
Jens Köllermann ◽  
Mark Schrader ◽  
Bernd Straub ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
High Specificity ◽  
Rt Pcr ◽  
Benign Lesions

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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