Comparison of Quantitative RT-PCR with Cell Culture to Detect Viral Hemorrhagic Septicemia Virus (VHSV) IVb Infections in the Great Lakes

2010 ◽  
Vol 22 (1) ◽  
pp. 50-61 ◽  
Author(s):  
Kristine M. Hope ◽  
Rufina N. Casey ◽  
Geoffrey H. Groocock ◽  
Rodman G. Getchell ◽  
Paul R. Bowser ◽  
...  
Keyword(s):  
Cell Culture ◽  
Great Lakes ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Rt Pcr ◽  
Viral Hemorrhagic Septicemia

Restricted replication of viral hemorrhagic septicemia virus (VHSV) in a birnavirus-carrier cell culture

2016 ◽  
Vol 162 (4) ◽  
pp. 1037-1041 ◽  
Author(s):  
Ricardo Parreño ◽  
Lucía Almagro ◽  
Melissa Belló-Pérez ◽  
Regla M. Medina-Gali ◽  
Amparo Estepa ◽  
...  
Keyword(s):  
Cell Culture ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Viral Hemorrhagic Septicemia ◽  
Carrier Cell

scholarly journals Molecular Epidemiology of Novirhabdoviruses Emerging in Iranian Trout Farms

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 448
Author(s):  
Sohrab Ahmadivand ◽  
Dušan Palić ◽  
Manfred Weidmann
Keyword(s):  
Amino Acids ◽  
Haplotype Analysis ◽  
Economic Losses ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Rt Pcr ◽  
Necrosis Virus ◽  
Viral Hemorrhagic Septicemia ◽  
Pcr Assays ◽  
Clade 1 ◽  
Trout Farms

Novirhabdoviruses cause large epizootics and economic losses of farmed trout. In this study, we surveyed Viral hemorrhagic septicemia virus and Infectious hematopoietic and necrosis virus (VHSV and IHNV) through both monitoring and investigation of clinical outbreaks reported by farmers in the regions with major rainbow trout production in Iran from 2015 to 2019. RT-PCR assays of the kidney samples and cell culture (EPC/FHM cells) samples confirmed the presence of the viruses, with 9 VHSV and 4 IHNV isolates, in both endemic and new areas of Iran. Sequence analysis of the G gene revealed that VHSV isolates belonged to genogroup Ia, and IHNV isolates were clustered into genogroup E, both typical for isolates from European countries. A haplotype analysis based on non-homologous amino acids of the G gene supports the emergence of two lineages of IHNV from clade 1 (E-1), as well as VHSV clade 2 (Ia-2) of the European genogroups, confirming that VHSV and IHNV isolates in Iran, have originated from Europe possibly via imported eggs.

scholarly journals Distribution of an Invasive Aquatic Pathogen (Viral Hemorrhagic Septicemia Virus) in the Great Lakes and Its Relationship to Shipping

PLoS ONE ◽  
2010 ◽  
Vol 5 (4) ◽  
pp. e10156 ◽  
Author(s):  
Mark B. Bain ◽  
Emily R. Cornwell ◽  
Kristine M. Hope ◽  
Geofrey E. Eckerlin ◽  
Rufina N. Casey ◽  
...  
Keyword(s):  
Great Lakes ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Viral Hemorrhagic Septicemia ◽  
Aquatic Pathogen
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