Edwardsiella piscicida identified in the southeastern USA by gyrB sequence, species-specific and repetitive sequence-mediated PCR

2014 ◽  
Vol 108 (1) ◽  
pp. 23-35 ◽  
Author(s):  
MJ Griffin ◽  
C Ware ◽  
SM Quiniou ◽  
JM Steadman ◽  
PS Gaunt ◽  
...  
Keyword(s):  
Repetitive Sequence ◽  
Southeastern Usa ◽  
Gyrb Sequence ◽  
Edwardsiella Piscicida ◽  
Species Specific

scholarly journals Pheromone Production, Attraction, and Interspecific Inhibition among Four Species ofIpsBark Beetles in the Southeastern USA

2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Göran Birgersson ◽  
Mark J. Dalusky ◽  
Karl E. Espelie ◽  
C. Wayne Berisford
Keyword(s):  
Sympatric Species ◽  
Pheromone Component ◽  
Pheromone Production ◽  
Field Bioassay ◽  
Southeastern Usa ◽  
Pheromone Components ◽  
Set Up ◽  
Species Specific ◽  
Potential Inhibitors ◽  
Trap Catches

Hindgut volatiles from attacking, unmated males ofIps avulsus, I. calligraphus, I. grandicollis, andI. piniwere analyzed by combined gas chromatography and mass spectrometry. Based on the quantitative identifications of hindguts and subsequent individual aerations, baits were formulated and a combined species-specific subtractive field bioassay was set up for the four bark beetle species. The bioassays were subtractive for the compounds identified in the hindgut analysis of each species, and volatiles identified in sympatric species were added as potential inhibitors alone and in combination. The trap catches from this bioassay revealed strong interspecific inhibition. The subtractive assays showed thatI. grandicollisandI. calligraphusshare (–)-(4S)-cis-verbenol as one pheromone component, while their second, synergistic pheromone component, (–)-(S)-ipsenol inI. grandicollisand (±)-ipsdienol inI. calligraphus, acts as an interspecific inhibitor to the other species.I. avulsusandI. piniwere found to have very similar production of hindgut volatiles, and both use ipsdienol and lanierone as synergistic pheromone components. No beetle-produced interspecific inhibitor was identified between these two species. Lanierone was found to be an interspecific inhibitor for bothI. calligraphusandI. grandicollis.

Differential Diagnosis of Taenia saginata and Taenia solium Infection by PCR

2000 ◽  
Vol 38 (2) ◽  
pp. 737-744 ◽  
Author(s):  
Luis Miguel González ◽  
Estrella Montero ◽  
Leslie J. S. Harrison ◽  
R. Michael E. Parkhouse ◽  
Teresa Garate
Keyword(s):  
Repetitive Sequence ◽  
Genomic Dna ◽  
Genomic Library ◽  
Taenia Solium ◽  
Differential Detection ◽  
Taenia Saginata ◽  
Noncoding Dna ◽  
Dna Fragment ◽  
Specific Fragment ◽  
Species Specific

We have designed species-specific oligonucleotides which permit the differential detection of two species of cestodes, Taenia saginata and Taenia solium. The oligonucleotides contain sequences established for two previously reported, noncoding DNA fragments cloned from a genomic library of T. saginata. The first, which is T. saginata specific (fragment HDP1), is a repetitive sequence with a 53-bp monomeric unit repeated 24 times in direct tandem along the 1,272-bp fragment. From this sequence the two oligonucleotides that were selected (oligonucleotides PTs4F1 and PTs4R1) specifically amplified genomic DNA (gDNA) from T. saginata but not T. solium or other related cestodes and had a sensitivity down to 10 pg of T. saginata gDNA. The second DNA fragment (fragment HDP2; 3,954 bp) hybridized to bothT. saginata and T. solium DNAs and was not a repetitive sequence. Three oligonucleotides (oligonucleotides PTs7S35F1, PTs7S35F2, and PTs7S35R1) designed from the sequence of HDP2 allowed the differential amplification of gDNAs from T. saginata, T. solium, and Echinococcus granulosus in a multiplex PCR, which exhibits a sensitivity of 10 pg.

scholarly journals Efficient anchoring of Erianthus arundinaceus chromatin introgressed into sugarcane by specific molecular markers

2020 ◽  
Author(s):  
Yongji Huang ◽  
Jiayun Wu ◽  
Xueting Li ◽  
Fan Yu ◽  
Xuguang Hu ◽  
...  
Keyword(s):  
Molecular Markers ◽  
High Throughput ◽  
Repetitive Sequence ◽  
Repetitive Sequences ◽  
Distribution Patterns ◽  
Subtractive Hybridization ◽  
High Accuracy ◽  
Wild Relatives ◽  
45S Rdna ◽  
Species Specific

Abstract Background: Erianthus arundinaceus is a valuable gene reservoir for sugarcane improvement. However, insufficient molecular markers for high-accuracy identification and tracking of the introgression status of E. arundinaceus chromatin impede sugarcane breeding. Fortunately, suppression subtractive hybridization (SSH) technology provides an excellent opportunity for development of high-throughput E. arundinaceus-specific molecular markers at a reasonable cost. Results: In this study, we constructed a SSH library of E. arundinaceus. In total, 288 clones E. arundinaceus-specific repetitive sequences were screened out and their distribution patterns on chromosomes were characterized by fluorescence in situ hybridization (FISH). A subtelomeric repetitive sequence Ea086 and a diffusive repetitive sequence Ea009, plus 45S rDNA-bearing E. arundinaceus chromosome repetitive sequence EaITS were developed as E. arundinaceus-specific molecular markers, namely Ea086-128, Ea009-257, and EaITS-278, covering all the E. arundinaceus chromosomes for high-accuracy identification of putative progeny. Both Ea086-128 and Ea009-257 were successfully applied to identify the authenticity of F1, BC1, BC2, BC3, and BC4 progeny between sugarcane and E. arundinaceus. In addition, EaITS-278 was a 45S rDNA-bearing E. arundinaceus chromosome-specific molecular marker for rapid tracking the inherited status of this chromosome in sugarcane background. Three BC3 progeny had apparently lost the 45S rDNA-bearing E. arundinaceus chromosome. Conclusions: We reported herein a highly effective and reliable SSH-based technology for discovery of high-throughput E. arundinaceus-specific sequences bearing high potential as molecular markers. Given its reliability and savings in time and efforts, the method is also suitable for development of species-specific molecular markers for other important wild relatives to accelerate introgression of wild relatives into sugarcane.

Genetic heterogeneity amongVibrio alginolyticusstrains, and design of a PCR-based identification method usinggyrBgene sequence

2018 ◽  
Vol 64 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Supansa Bunpa ◽  
Mitsuaki Nishibuchi ◽  
Jumroensri Thawonsuwan ◽  
Natthawan Sermwittayawong
Keyword(s):  
Genetic Diversity ◽  
Chain Reaction ◽  
Marine Animals ◽  
Gyrb Sequence ◽  
Highly Sensitive ◽  
Southern Thailand ◽  
Polymerase Chain ◽  
Species Specific ◽  
Dna Template ◽  
Vibrio Spp

Vibrio alginolyticus, a pathogen among humans and marine animals, is ubiquitous in marine environments. The aims of this study were to analyze the relationships between genetic diversity and origins, and to develop new primers based on the gyrB sequence to identify V. alginolyticus isolated from various sources. To determine the genetic diversity of this bacterium, an arbitrarily primed polymerase chain reaction (AP-PCR) technique was performed on 36 strains of V. alginolyticus isolated from diarrhea patients and from diseased marine animals and environments in southern Thailand. The results showed distinct DNA fingerprints of all strains, indicating that they are genetically heterogeneous. For species-specific identification of V. alginolyticus, primers targeting the gyrB gene of V. alginolyticus were developed. Thirty reference Vibrio spp., 13 non-Vibrio spp., and 160 strains of V. alginolyticus isolated from various sources in southern Thailand were used to evaluate the specificity of these primers. Our results showed that the gyrB primers could specifically identify V. alginolyticus from all sample types. In addition, the detection limit of the PCR was at least 95 pg of DNA template. Therefore, we concluded that the newly designed gyrB primers are rapid, highly sensitive, and specific to identify V. alginolyticus isolated from various sources.

Isolation of a new species-specific repetitive sequence from Thinopyrum elongatum and its use in the studies of alien translocations

Genome ◽  
1994 ◽  
Vol 37 (1) ◽  
pp. 97-104 ◽  
Author(s):  
B. Bournival ◽  
M. Obanni ◽  
A. Abad ◽  
H. Ohm ◽  
S. Mackenzie
Keyword(s):  
In Situ Hybridization ◽  
Repetitive Sequence ◽  
Triticum Aestivum L ◽  
Deletion Mapping ◽  
Agropyron Elongatum ◽  
Low Copy Number ◽  
Species Specific ◽  
Thinopyrum Elongatum

A new repetitive sequence that is extremely abundant and well dispersed in the Thinopyrum elongatum genome but present in low-copy number in wheat (Triticum aestivum L.) has been isolated. This repeat and a Th. elongatum repeat isolated in another laboratory were used to identify cosmid genomic clones containing the repeats and, thus, putatively located on a Th. elongatum/T. aestivum translocation arm. Most of the selected cosmids contained single-or low-copy sequences, making them potentially useful in mapping studies. The repeats were used in deletion mapping to deduce gene order of three genes located on the Th. elongatum translocation arm. In situ hybridization studies suggested that this newly identified Th. elongatum repeat is well dispersed throughout the Thinopyrum genome but present at only one location in wheat. This raises some interesting questions about the role of such repetitive elements in the evolution of grass species.Key words: species-specific repeats, wheat, wheatgrass, Agropyron elongatum, in situ hybridization.

Electron Microscopy in the Study of Natural Phytoplankton Assemblages

1985 ◽  
Vol 43 ◽  
pp. 620-623
Author(s):  
Linda Sicko-Goad
Keyword(s):  
Electron Microscopy ◽  
Aquatic Environment ◽  
Size Range ◽  
Physical Parameters ◽  
Specific Information ◽  
Phytoplankton Assemblages ◽  
Phytoplankton Productivity ◽  
Ecosystem Effects ◽  
Time Of Year ◽  
Species Specific

Although the use of electron microscopy and its varied methodologies is not usually associated with ecological studies, the types of species specific information that can be generated by these techniques are often quite useful in predicting long-term ecosystem effects. The utility of these techniques is especially apparent when one considers both the size range of particles found in the aquatic environment and the complexity of the phytoplankton assemblages.The size range and character of organisms found in the aquatic environment are dependent upon a variety of physical parameters that include sampling depth, location, and time of year. In the winter months, all the Laurentian Great Lakes are uniformly mixed and homothermous in the range of 1.1 to 1.7°C. During this time phytoplankton productivity is quite low.

64: Rapid, Species-Specific Detection of Uropathogens from Clinical Urine Specimens Using an Electrochemical Microsensor Array

2005 ◽  
Vol 173 (4S) ◽  
pp. 18-18
Author(s):  
Joseph C. Liao ◽  
Mitra Mastali ◽  
David A. Haake ◽  
Bernard M. Churchill
Keyword(s):  
Specific Detection ◽  
Species Specific ◽  
Urine Specimens
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