scholarly journals Multiplex PCR for the detection of Piscirickettsia salmonis, Vibrio anguillarum, Aeromonas salmonicida and Streptococcus phocae in Chilean marine farms

2011 ◽  
Vol 97 (2) ◽  
pp. 135-142 ◽  
Author(s):  
D Tapia-Cammas ◽  
A Yañez ◽  
G Arancibia ◽  
AE Toranzo ◽  
R Avendaño-Herrera
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Anguillarum ◽  
Aeromonas Salmonicida ◽  
Piscirickettsia Salmonis

Ultraviolet Treatment of Water for Destruction of Five Gram-Negative Bacteria Pathogenic to Fishes

1977 ◽  
Vol 34 (8) ◽  
pp. 1244-1249 ◽  
Author(s):  
G. L. Bullock ◽  
H. M. Stuckey
Keyword(s):  
Particulate Matter ◽  
Ultraviolet Irradiation ◽  
Vibrio Anguillarum ◽  
Spring Water ◽  
Aeromonas Salmonicida ◽  
Water Disinfection ◽  
Gram Negative Bacteria ◽  
Fish Pathogens ◽  
Gram Negative ◽  
Ultraviolet Treatment

Filtration (25 nm) and ultraviolet irradiation dosages of 13,100–29,400 microwatt seconds per square centimetre (μW∙s∙cm−2) effected a 99.98–100% reduction of five gram-negative fish pathogens — Aeromonas salmonicida, A. hydrophila, Vibrio anguillarum, Pseudomonas fluorescens, and the enteric redmouth organism in 12.5 °C clear spring water or spring water containing particulate matter. Filtration and a dosage of 4500 μW∙s∙cm−2 killed 99.83–100% of test strains in spring water and 4000–4750 μW∙s∙cm−2 killed 99.33–99.99% in water with particulate matter. Irradiation of unfiltered water containing particulate matter was less effective, especially at dosages of 5000 μW∙s∙cm−2 or less, which killed 97–99.94% of strains. Filtration and 13,100 μW∙s∙cm−2 irradiation of water containing A. salmonicida prevented transmission of furunculosis. Key words: ultraviolet irradiation, bacterial fish pathogens, water disinfection

Application of real-time PCR for early diagnosis of diseases caused by Aeromonas salmonicida, Vibrio anguillarum, and Tenacibaculum maritimum in turbot: A field study

2017 ◽  
Vol 30 (1) ◽  
pp. 76-89 ◽  
Author(s):  
María-José Chapela ◽  
Martiña Ferreira ◽  
Asela Ruiz-Cruz ◽  
Iris Martin-Varela ◽  
Jacobo Fernández-Casal ◽  
...  
Keyword(s):  
Early Diagnosis ◽  
Field Study ◽  
Real Time ◽  
Real Time Pcr ◽  
Vibrio Anguillarum ◽  
Aeromonas Salmonicida ◽  
Diagnosis Of Diseases

Multiplex PCR for simultaneous detection of five virulence hemolysin genes in Vibrio anguillarum

2006 ◽  
Vol 65 (3) ◽  
pp. 612-618 ◽  
Author(s):  
Channarong Rodkhum ◽  
Ikuo Hirono ◽  
Jorge H. Crosa ◽  
Takashi Aoki
Keyword(s):  
Multiplex Pcr ◽  
Simultaneous Detection ◽  
Vibrio Anguillarum

scholarly journals Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon

Biology ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 82
Author(s):  
Tiril H. Slettjord ◽  
Hege J. Sekkenes ◽  
Heng Chi ◽  
Jarl Bøgwald ◽  
Trilochan Swain ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Bacterial Infections ◽  
Vibrio Anguillarum ◽  
Inflammatory Cells ◽  
Mrna Levels ◽  
Protective Effects ◽  
Cell Nuclei ◽  
Post Challenge ◽  
Ifn Γ ◽  
Piscirickettsia Salmonis

The overexpression of GATA-3, T-bet and TGF-ß may theoretically induce IL-4/A, IFN-γ and IL-17A expression, respectively. Whether this also applies to fish is not yet known. The plasmid vectors encoding reporter gene (RFP)-tagged T-bet, GATA-3 and TGF-ß were used as overexpression tools, transfected into cells or injected intramuscularly to monitor the expression of IFN-γ, IL-4/13A and IL-17A. In addition, the fish were either experimentally challenged with Vibrio anguillarum (VA group) or Piscirickettsia salmonis (PS group). The reporter gene (RFP) inserted upstream of the GATA-3, T-bet and TGF-ß genes, was observed in muscle cell nuclei and in inflammatory cells after intramuscular (i.m.) injection. PS group: following the injection of GATA-3 and T-bet-encoding plasmids, the expression of GATA-3 and T-bet was high at the injection site. The spleen expression of IFN-γ, following the injection of a T-bet-encoding plasmid, was significantly higher on day 2. VA group: The T-bet and GATA-3-overexpressing fish expressed high T-bet and GATA-3 mRNA levels in the muscles and on day 4 post-challenge. The expression of TGF-ß in the muscles of fish injected with TGF-ß-encoding plasmids was significantly higher on days 7 (8 days pre-challenge) and 19 (4 days after challenge). The protective effects of the overexpression of T-bet, GATA-3 and TGF-ß on both bacterial infections were negligible.

Bacterial adhesion to stainless steel is reduced by aqueous fish extract coatings

Biofilms ◽  
2006 ◽  
Vol 3 (1) ◽  
pp. 25-36 ◽  
Author(s):  
N. Bernbom ◽  
R. L. Jørgensen ◽  
Y. Y. Ng ◽  
R. L. Meyer ◽  
P. Kingshott ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Photoelectron Spectroscopy ◽  
Bacterial Adhesion ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Vibrio Anguillarum ◽  
Aeromonas Salmonicida ◽  
Bacterial Attachment ◽  
Stainless Steel Surface ◽  
Flight Mass Spectrometry

ABSTRACTMicrobial adhesion and biofilm formation on surfaces pose major problems and risks to human health. One way to circumvent this problem is to coat surfaces (in this report stainless steel) with a non-toxic fish extract that generates an abiotic surface with less bacterial attachment than uncoated surfaces or surfaces coated with, for example, tryptone soy broth. The bacteria grow well in the fish extract; hence a general bacteriocidal effect is not the reason for the antifouling effect. Bacterial attachment was quantified by different methods including (a) direct fluorescence microscopy, (b) removal by ultrasound and subsequent quantification of the adhered bacteria, and (c) regrowth of the adhered bacteria measured by indirect conductometry. Surprisingly, the bacterial counts on surfaces coated with aqueous fish extract were 10–100 times lower than on surfaces coated with laboratory broths when surfaces were submerged in bacterial suspensions. The effect was seen forPseudomonas fluorescensAH2,Pseudomonas aeruginosaPAO1,Escherichia coliMG1655,Vibrio anguillarum90-11-287 andAeromonas salmonicidaJno 3175/88. It lasted for at least 7 days. Atomic force microscopy showed that steel surfaces conditioned with fish extract were covered by a thin layer of spherical, nanosized particles. Chemical analysis of the surfaces coated with adsorbed fish extract using X-ray photoelectron spectroscopy revealed that the layer was proteinaceous and had a thickness less than 2 nm. Numerous protein bands/peaks were also detected by sodium dodecyl sulphate–polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry techniques. We conclude that coating the stainless steel surface with fish extract results in a thin protein layer that reduces bacterial adhesion significantly.

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