scholarly journals Development of a new multiplex-PCR tool for the simultaneous detection of the fish pathogens Vibrio alginolyticus, Vibrio anguillarum, Vibrio harveyi and Edwardsiella tarda

2017 ◽  
Vol 30 ◽  
pp. 4 ◽  
Author(s):  
Micaela Ferreira Pinto ◽  
Teresa Baptista ◽  
Clélia Correia Neves Afonso
Keyword(s):  
Multiplex Pcr ◽  
Vibrio Harveyi ◽  
Simultaneous Detection ◽  
Vibrio Anguillarum ◽  
Vibrio Alginolyticus ◽  
Edwardsiella Tarda ◽  
Fish Pathogens

scholarly journals Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquaculture

2018 ◽  
Vol 53 (2) ◽  
pp. 171 ◽  
Author(s):  
Mohammed Ishaque Nabila ◽  
Krishnan Kannabiran
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Vibrio Harveyi ◽  
Bacterial Pathogens ◽  
Vibrio Anguillarum ◽  
Antagonistic Activity ◽  
Edwardsiella Tarda ◽  
Streptomyces Sp ◽  
Aeromonas Caviae ◽  
Fish And Shellfish

A total of 72 morphologically different actinomycetes isolates were isolated from samples collected at different regions of Vellore, Tamil Nadu, India and screened for its antibacterial activity against fish and shellfish pathogens. All actinomycetes isolates were screened for antibacterial activity by cross streak method against the selected fish and shellfish bacterial pathogens including Aeromonas caviae, Aeromonas hydroplila, Edwardsiella tarda, Vibrio anguillarum and Vibrio harveyi. Secondary screening of antagonistic isolates by well diffusion method leads to the identification of potential isolate. Culture conditions for the potential isolate were optimized for maximal growth and yield of the ethyl acetate (EA) crude extract. The potential isolate was characterized by molecular taxonomy and phylogeny and identified as Streptomyces species and named as Streptomyces sp. VITNK9. The 16S rDNA nucleotide sequence was searched through the GenBank database and showed 83% similarity to Streptomyces vinaceusdrappus. The EA extract prepared from Streptomyces sp. VITNK9 showed moderate antagonistic activity accessed by the formation of zone of growth inhibition against, Aeromonas caviae (15.33 mm), Aeromonas hydrophila (17.66 mm), Edwardsiella tarda (18.33 mm), Vibrio anguillarum (14.33 mm) and Vibrio harveyi (14.33 mm). The MIC value of EA extract was ranged between 0.03-0.125 mg mL-1. The GC-MS spectrum of the ethyl acetate extract revealed the presence of two major compounds, pyrrolo [1,2-A] pyrazine-1,4-Dione (56.67%) and Hexahydro-3-(2-Methylpropyl) (27.91%), respectively. The results of the study suggest that Streptomyces sp. VITNK9 is a potential source for antagonistic secondary metabolites against fish and shellfish bacterial pathogens.

Multiplex PCR for simultaneous detection of five virulence hemolysin genes in Vibrio anguillarum

2006 ◽  
Vol 65 (3) ◽  
pp. 612-618 ◽  
Author(s):  
Channarong Rodkhum ◽  
Ikuo Hirono ◽  
Jorge H. Crosa ◽  
Takashi Aoki
Keyword(s):  
Multiplex Pcr ◽  
Simultaneous Detection ◽  
Vibrio Anguillarum

scholarly journals Species-Specific Duplex PCR for Detecting the Important Fish Pathogens Vibrio anguillarum and Edwardsiella tarda

2013 ◽  
Vol 16 (4) ◽  
pp. 273-277 ◽  
Author(s):  
Geon-A Jo ◽  
Sae-Bom Kwon ◽  
Na-Kyeong Kim ◽  
Muhammad Tofazzal Hossain ◽  
Yu-Ri Kim ◽  
...  
Keyword(s):  
Vibrio Anguillarum ◽  
Edwardsiella Tarda ◽  
Duplex Pcr ◽  
Fish Pathogens ◽  
Species Specific ◽  
Important Fish

scholarly journals Simultaneous Detection of Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri, Three Major Fish Pathogens, by Multiplex PCR

2002 ◽  
Vol 68 (10) ◽  
pp. 5177-5180 ◽  
Author(s):  
A. del Cerro ◽  
I. Marquez ◽  
J. A. Guijarro
Keyword(s):  
Multiplex Pcr ◽  
Simultaneous Detection ◽  
Aeromonas Salmonicida ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Yersinia Ruckeri ◽  
Pcr Assay ◽  
Fish Pathogens ◽  
Flavobacterium Psychrophilum ◽  
Multiplex Pcr Assay

ABSTRACT A multiplex PCR assay based on the 16S rRNA genes was developed for the simultaneous detection of three major fish pathogens, Aeromonas salmonicida, Flavobacterium psychrophilum, and Yersinia ruckeri. The assay proved to be specific and as sensitive as each single PCR assay, with detection limits in the range of 6, 0.6, and 27 CFU for A. salmonicida, F. psychrophilum, and Y. ruckeri, respectively. The assay was useful for the detection of the bacteria in artificially infected fish as well as in fish farm outbreaks. Results revealed that this multiplex PCR system permits a specific, sensitive, reproducible, and rapid method for the routine laboratory diagnosis of infections produced by these three bacteria.

Identification of Pathogenic Fish Bacteria Using the API ZYM System

1993 ◽  
Vol 50 (6) ◽  
pp. 1137-1141 ◽  
Author(s):  
Masahiro Sakai ◽  
M. K. Soliman ◽  
T. Yoshida ◽  
Masanori Kobayashi
Keyword(s):  
Bacterial Species ◽  
Vibrio Anguillarum ◽  
Aeromonas Salmonicida ◽  
Edwardsiella Tarda ◽  
Gram Stain ◽  
Fish Pathogen ◽  
Fish Pathogens ◽  
Renibacterium Salmoninarum ◽  
Hemolytic Streptococcus ◽  
Fish Bacteria

The API ZYM system was used to identify the bacterial fish pathogens Enterococcus seriolicida, β-hemolytic Streptococcus sp., Renibacterium salmoninarum, Aeromonas salmonicida, A. hydrophila, Pasteurella piscicida, Edwardsiella tarda, Vibrio anguillarum, Pseudomonas fluorescens, Flexibacter columnaris, and F. maritimus. As additional tests, Gram stain and the activities of cytochrome oxidase and catalase were examined. The results of APS ZYM and the additional tests were coded for easy identification. Several biotypes were demonstrated by Streptococcus sp., V. anguillarum, and A. hydrophila. Other bacteria showed uniform profiles. This system can distinguish each fish pathogen from all other bacterial species examined.

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