scholarly journals Estimation of the fate of dissolved DNA in thermally stratified lake water from the stability of exogenous plasmid DNA

2001 ◽  
Vol 26 ◽  
pp. 95-102 ◽  
Author(s):  
K Matsui ◽  
M Honjo ◽  
Z Kawabata
Keyword(s):  
Lake Water ◽  
Plasmid Dna ◽  
Stratified Lake ◽  
The Stability ◽  
Exogenous Plasmid

Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation

1991 ◽  
Vol 11 (4) ◽  
pp. 2328-2332
Author(s):  
L E Brown ◽  
S L Sprecher ◽  
L R Keller
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Plasmid Dna ◽  
Cell Walls ◽  
Intact Cell ◽  
Exogenous Dna ◽  
Electrical Pulses ◽  
Exogenous Plasmid

The fate of exogenous DNA introduced into Chlamydomonas reinhardtii by electroporation was analyzed. With single and double electrical pulses, plasmids as large as 14 kb were introduced into cells with and without intact cell walls. Within hours after introduction, exogenous plasmid DNA was associated with nuclei isolated from cells; several weeks after introduction, exogenous DNA was stably integrated into the Chlamydomonas genome. These studies establish electroporation as a method for introducing DNA, and potentially other molecules, into C. reinhardtii.

scholarly journals Asbestos fibers mediate transformation of monkey cells by exogenous plasmid DNA.

1988 ◽  
Vol 85 (20) ◽  
pp. 7670-7674 ◽  
Author(s):  
J. D. Appel ◽  
T. M. Fasy ◽  
D. S. Kohtz ◽  
J. D. Kohtz ◽  
E. M. Johnson
Keyword(s):  
Plasmid Dna ◽  
Asbestos Fibers ◽  
Exogenous Plasmid

scholarly journals Retrieval of Particle Scattering Coefficients and Concentrations by Genetic Algorithms in Stratified Lake Water

2014 ◽  
Vol 6 (10) ◽  
pp. 9530-9551 ◽  
Author(s):  
Jaime Pitarch ◽  
Daniel Odermatt ◽  
Marcin Kawka ◽  
Alfred Wüest
Keyword(s):  
Genetic Algorithms ◽  
Lake Water ◽  
Particle Scattering ◽  
Stratified Lake ◽  
Scattering Coefficients

scholarly journals Enhanced Agrobacterium ‐mediated transformation revealed attenuation of exogenous plasmid DNA installation in recipient bacteria by exonuclease VII and SbcCD

2020 ◽  
Vol 25 (10) ◽  
pp. 663-674
Author(s):  
Kazuya Kiyokawa ◽  
Yuta Ohmine ◽  
Kazuya Yunoki ◽  
Shinji Yamamoto ◽  
Kazuki Moriguchi ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Agrobacterium Mediated Transformation ◽  
Exonuclease Vii ◽  
Exogenous Plasmid

scholarly journals Stability Assessment and Optimization Design of Lakeside Open-Pit Slope considering Fluid-Solid Coupling Effect

2015 ◽  
Vol 2015 ◽  
pp. 1-11
Author(s):  
Wenchen Fan ◽  
Ping Cao ◽  
Ke Zhang ◽  
Kaihui Li ◽  
Chong Chen
Keyword(s):  
Slope Stability ◽  
Water Level ◽  
Lake Water ◽  
Optimization Design ◽  
Great Influence ◽  
Water Levels ◽  
Open Pit ◽  
Stability Of Slopes ◽  
The Stability ◽  
Slope Design

Chengmenshan copper mine, located at Jiujiang city in the Jiangxi Province, is a rarely lakeside open-pit mine in China. Since the open-pit is very close to Sai Lake, the seasonally changed water level and the distance between lake and slope have great influence to the stability of open-pit slope. Based on the drill data and geological sections, a numerical model of the slope is built. With the fluid-mechanical interaction associated, the stability of the slopes is numerically analyzed, in which different lake water levels and lake-slope distances are taken into consideration. The comparative analysis shows that a larger lake-slope distance can promise better slope stability and weaken the sensitivity of slope stability to water. The stability of slopes with different heights is analyzed to find that the stability weakens and the sensitivity is enhanced with the height increasing. To the most serious situation, the slope height and the lake water level being 238 m and 17.2 m, respectively, theFsvalue equals 1.18945 which is extremely closed to the allowable safety factor of 1.20 for slope design. According to the minimumFsfor slope design, the minimum distance between lake and open-pit slope is found to be 60 m.

On the stability of plasmid DNA vectors during cell culture and purification

2007 ◽  
Vol 36 (2) ◽  
pp. 151-158 ◽  
Author(s):  
S. S. Freitas ◽  
A. R. Azzoni ◽  
J. A. L. Santos ◽  
G. A. Monteiro ◽  
D. M. F. Prazeres
Keyword(s):  
Cell Culture ◽  
Plasmid Dna ◽  
The Stability ◽  
Dna Vectors
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