The effect of protective agents on the stability of plasmid DNA by the process of spray-drying

2003 ◽  
Vol 55 (3) ◽  
pp. 301-306 ◽  
Author(s):  
Jung-hua Steven Kuo
Keyword(s):  
Spray Drying ◽  
Plasmid Dna ◽  
Protective Agents ◽  
The Stability

Influence of spray drying on the stability of food-grade solid lipid nanoparticles

2019 ◽  
Vol 119 ◽  
pp. 741-750 ◽  
Author(s):  
Hanna Salminen ◽  
Juliane Ankenbrand ◽  
Benjamin Zeeb ◽  
Gabriela Badolato Bönisch ◽  
Christian Schäfer ◽  
...  
Keyword(s):  
Spray Drying ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Food Grade ◽  
Solid Lipid ◽  
The Stability

Influence of the emulsion homogenization method on the stability of chia oil microencapsulated by spray drying

2019 ◽  
Vol 354 ◽  
pp. 877-885 ◽  
Author(s):  
Maristela Alves Alcântara ◽  
Anderson Eduardo Alcântara de Lima ◽  
Ana Luiza Mattos Braga ◽  
Renata Valeriano Tonon ◽  
Melicia Cintia Galdeano ◽  
...  
Keyword(s):  
Spray Drying ◽  
Homogenization Method ◽  
The Stability ◽  
Chia Oil

Microencapsulation of Colors by Spray Drying - A Review

2012 ◽  
Vol 8 (2) ◽  
Author(s):  
Kannan Kandansamy ◽  
Priyenka Devi Somasundaram
Keyword(s):  
Spray Drying ◽  
Quality Indicator ◽  
Milk Protein ◽  
Cost Effective ◽  
Effective Technique ◽  
Food Ingredients ◽  
Natural Pigments ◽  
Natural Colorants ◽  
Cost Effective Technique ◽  
The Stability

Color is one of the important attributes of foods being considered as a quality indicator and determining frequently their acceptance. Many natural pigments like anthocyanin, lycopenes, betalains etc., are subjected to color loss during storage. Hence in order to increase the stability of the natural colorants the technology of microencapsulation is involved. Microencapsulation has been used in recent years for the encapsulation of food ingredients such as flavours, colors, lipids and milk protein. Among various methods used for microencapsulation of colors, the technically feasible and cost effective technique is spray drying. This paper reviews the various concepts of spray drying and various technologies involved in microencapsulation which are considered to be useful parameters for increasing the stability of natural colorants. It also summarizes the most commonly used natural colorants which can be encapsulated.

Development of a rapid screening protocol for selection of strains resistant to spray drying and storage in dry powder

2010 ◽  
Vol 1 (2) ◽  
pp. 165-174 ◽  
Author(s):  
S. Reimann ◽  
F. Grattepanche ◽  
C. Baggenstos ◽  
E. Rezzonico ◽  
B. Berger ◽  
...  
Keyword(s):  
Spray Drying ◽  
Bifidobacterium Longum ◽  
Survival Rates ◽  
Screening Method ◽  
Rapid Screening ◽  
Fast Screening ◽  
Screening Protocol ◽  
The Stability ◽  
And Storage ◽  
Selection Of

An efficient screening method for selection of Bifidobacterium longum strains resistant to spray drying and storage was developed based on randomly amplified polymorphic DNA (RAPD) for identification of the best survivors in mixed strains bacterial preparations. Three different primers were used to generate RAPD profiles of 22 B. longum strains. All strains were distinguished according to their RAPD profiles except for the strain NCC2705 and its H2O2 resistant derivative variant. The 22 strains were grouped in 3 batches of 7, 7 and 8 strains and subjected to spray drying and storage at 30 and 37 °C under anaerobic conditions. Batch survival rates after spray drying reached 17.1±4.4%. Strains showing the highest prevalence and/or resistance to storage at 37 °C were selected from individual batches for subsequent spray drying and storage testing. After 67 days of storage, NCC572 was identified as the dominant strain in powder. The stability of strain NCC572 was confirmed by performing single spray drying and storage tests. Out of 22 B. longum strains, a robust strain was identified by combining RAPD with a simultaneous screening test for survival under spray drying and storage. The method allowed a fast screening of B. longum strains in mixture for resistance to spray drying and storage compared to traditional screening procedures carried out with individual strains, in the same conditions. This approach could be applied to other stress conditions.

scholarly journals Phenolic Compounds in Calafate Berries Encapsulated by Spray Drying: Neuroprotection Potential into the Ingredient

Antioxidants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1830
Author(s):  
María E. Romero-Román ◽  
Mauricio Schoebitz ◽  
Jorge Fuentealba ◽  
Cristina García-Viguera ◽  
María D. López Belchí
Keyword(s):  
Spray Drying ◽  
Antioxidant Activities ◽  
Value Added ◽  
Cost Effective ◽  
Biological Action ◽  
Natural State ◽  
Degenerative Disorders ◽  
The Stability ◽  
Berry Extracts

Calafate is a berry rich in anthocyanins that presents higher content of polyphenols than other fruits. Its compounds have been described previously, however, the potential thereof in preventing and treating degenerative disorders has not yet been studied. Due to its astringency, the consumption of this berry in its natural state is limited. To profit from the aforementioned properties and reduce palatability issues, calafate berry extracts were microencapsulated by spray drying, a rapid, cost-effective and scalable process, and were then compared with freeze drying as a control. The stability of its contents and its in-vitro potential, with respect to AChE activity and neuroprotection, were measured from the obtained microcapsules, resulting from temperature treatments and different encapsulant contents. The results indicated that the spray-dried powders were stable, despite high temperatures, and their encapsulation exhibited nearly 50% efficiency. The highest quantity of polyphenols and 3-O-glycosylated anthocyanins was obtained from encapsulation with 20% maltodextrin, at 120 °C. Temperature did not affect the microcapsules’ biological action, as demonstrated by their antioxidant activities. The prevention of Aβ peptide cytotoxicity in PC12 cells (20%) revealed that encapsulated calafate can confer neuroprotection. We conclude that spray-drying is an appropriate technique for scaling-up and producing new value-added calafate formulations with anti-neurodegenerative effects and vivid colors.

scholarly journals Produksi, isolasi dan karakterisasi superoksida dismutase dari Spirulina platensis yang dibiakkan dalam serum lateks Production, isolation, and characterization of superoxyde dismutase from Spirulina platensis cultured on latex serum

2016 ◽  
Vol 77 (1) ◽  
Author(s):  
. TRI-PANJI ◽  
. SUHARYANTO ◽  
Marini WIJAYANTI
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Spray Drying ◽  
Spirulina Platensis ◽  
Active Agent ◽  
Pilot Scale ◽  
Isolation And Characterization ◽  
The Stability ◽  
Major Amino Acid

AbstractSpirulina platensis is a blue-green microalgawhich is frequently used for food and feedsupplements and cosmetic active agent. Thismicroalga also produces a strong antioxidantnamely superoxide dismutase (SOD) used ascosmetic active agent for anti aging and anti freeradicals. SOD was isolated from S. platensis cellbiomass from local isolate grown on latex serumon semipilot (3.5 m 3 ) and pilot scale (40 m 3 )then dried with spray drying or sun drying andcharacterized. SOD was purified with sequentialtwo-stage sedimentation using ammoniumsulphate and fractionated in chromatographiccolumn containing Sephadex G 200. Thefractions were analysed to determine the activity,cofactor metal and amino acid composition of theantioxidant. The results showed thatsedimentation of SOD extract with 80%ammonium sulphate produced SOD with higheractivity compared to that of SOD fromcommercial S. platensis biomass. This SOD wassuccessfully isolated and purified. MetaloenzymeSOD was composed of subunits with molecularweight of 77.78; 71.74; and 19.2 kDa, whichcontained nine types of amino acids with tyrosineand lysine as the major amino acid components.Zn was the most predominant metal on SOD, thenfollowed by Fe and Mn. The main subunitcofactors consisted of Zn 72%, Fe 25%, Mn 2%,and Cu 1%, which were different from thesmall subunit that contained of Zn 55%, Mn 31%,Fe 14%, and Cu 4%. The stability of SOD wasachieved on pH 7.5 and temperature below 25 o C.AbstrakSpirulina platensis adalah mikroalga hijaubiru yang banyak digunakan sebagai suplemenpangan, pakan, dan bahan aktif kosmetika.Mikroalga ini juga menghasilkan antioksidankuat yaitu superoksida dismutase (SOD), yangmerupakan bahan aktif kosmetika anti penuaandini dan pencegah efek radikal bebas. SODdiisolasi dari biomassa sel S. platensis isolat lokalyang dibiakkan dalam serum lateks skalasemipilot (3,5 m 3 ) dan pilot (40 m 3 ) sertadikeringkan dengan cara pengeringan kabut(spray drying) atau penjemuran untuk kemudiandikarakterisasi. SOD dimurnikan dengan peng-endapan bertingkat menggunakan ammoniumsulfat dan dipisahkan dengan kolom kromatografiberisi Sephadex G 200. Hasil pemisahankemudian dianalisis untuk menentukan aktivitas,logam kofaktor serta komposisi asam amino antioksidan tersebut. Hasil penelitian menunjukkanbahwa pengendapan ekstrak SOD denganSOD lebih tinggi dari SOD asal biomassaS. platensis komersial. SOD tersebut telahberhasil diisolasi dan dimurnikan. MetaloenzimSOD tersusun atas subunit dengan BM 77,78;71,74; dan 19,2 kDa, yang mengandungsembilan jenis asam amino dengan tirosin danlisin sebagai komponen asam amino utama.Logam yang dominan pada SOD adalah Zn,disusul kemudian Fe dan Mn. Kofaktor sub unitbesar terdiri dari Zn 72%, Fe 25%, Mn 2%, danCu 1%, berbeda dengan sub unit kecil yangmengandung Zn 55%, Mn 31%, Fe 14%, dan Cu4%. Stabilitas SOD S. platensis dicapai pada pH7,5 dan suhu di bawah 25 o Cammonium sulfat 80% menghasilkan aktivitas

scholarly journals STABILITY OF MICROSPHERE OF SARGASSUM PLAGYOPHYLLUM (MERTENS) J. G. AGARDH EXTRACT PRODUCED BY SPRAY DRYING USING MALTODEXTRIN

2018 ◽  
Vol 10 (1) ◽  
pp. 376
Author(s):  
Effionora Anwar ◽  
Dyah Ayu Ratna Yulianti ◽  
Kurnia Sari Setio Putri
Keyword(s):  
Antioxidant Activity ◽  
Spray Drying ◽  
Brown Seaweed ◽  
Inlet Temperature ◽  
Aqueous Extracts ◽  
Size Distributions ◽  
Particle Size Distributions ◽  
Coating Agent ◽  
Polyphenol Compounds ◽  
The Stability

Objective: This study aimed to determine the stability of microspheres of Sargassum plagyophyllum (brown seaweed) after preparation using spraydrying with maltodextrin DE 10–15 and during drying and storage.Methods: Aqueous extracts of brown seaweed were formulated into microspheres using maltodextrin DE 10–15 as a coating agent. For increasing thestability of polyphenol compounds, spray drying was performed with an inlet temperature of 110°C. Four microsphere formulations were producedusing maltodextrin DE 10–15 at concentrations of 0%, 5%, 10%, and 15%. The resulting microspheres were then characterized in the assessments ofmoisture contents, particle size distributions, pH, total phlorotannin contents, and antioxidant activity, and surface morphology was analyzed usingscanning electron microscope analyses.Results: Powders that were produced with 0% and 15% maltodextrin were more stable at 4°C±2°C than at 28°C±2°C and 40°C±2°C. At the lowesttemperature, phlorotannin contents were maintained in powders that were prepared with 15% maltodextrin but were decreased by 10% in powdersthat were prepared without maltodextrin.Conclusion: Maltodextrin DE 10–15 is a suitable coating agent for dry formulations of S. plagyophyllum powder and maintained stability during spraydrying at 110°C and during storage for 2 months at 4°C±2°C.

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