scholarly journals Biochar-immobilized Sphingomonas sp. and Acinetobacter sp. isolates to enhance nutrient removal: potential application in crab aquaculture

2020 ◽  
Vol 12 ◽  
pp. 251-262
Author(s):  
Y Shao ◽  
H Zhong ◽  
X Mao ◽  
H Zhang

The frequency of water exchange and reducing the risk of eutrophication to surrounding water bodies have always been water-quality control issues in recirculating aquaculture systems. In this study, maize straw biochar prepared through pyrolysis showed great potential for both bacterial immobilization and pollutant adsorption. Heterotrophic bacterial strains of Sphingomonas sp. PDD-57b-25 and Acinetobacter towneri were isolated in situ from wastewater for pollutant remediation through a 16S rDNA-based method, which has been rarely reported to date. The selected strains had higher ammonia nitrogen (NH4+-N, 63%), nitrite nitrogen (NO2--N, 38%), nitrate nitrogen (NO3--N, 25%) and total phosphorus (TP, 35%) assimilation capacities than those of other widely applied bacteria under similar medium conditions. In addition, more NH4+-N (+16%), NO2--N (+14%), NO3--N (+17%) and TP (+19%) was removed by biochar-immobilized isolated strains than dissociated strains, suggesting their use may provide a means of improving water-quality control in recirculating aquaculture. With specific additions (4 g l-1) of biochar-immobilized Sphingomonas sp. PDD-57b-25 and A. towneri, the dissolved inorganic nitrogen (approximately 0.45 mg l-1) and TP (approximately 0.09 mg l-1) levels were maintained below the clean water threshold for recirculating aquaculture of crab Eriocheir sinensis. Furthermore, the added strains exhibited high bio-safety and were capable of improving the yield and quality of crabs. Results indicate the potential applicability of biochar-immobilized Sphingomonas sp. PDD-57b-25 and A. towneri in agricultural sewage treatments. Further, the experimental methodology developed here may be used for the exploration of new strains for practical aquaculture.

Author(s):  
Carlos A. Espinal ◽  
Daniel Matulić

AbstractRecirculating aquaculture technology, which includes aquaponics, has been under development for the past 40 years from a combination of technologies derived from the wastewater treatment and aquaculture sectors. Until recently, recirculating aquaculture systems (RAS) farms have been relatively small compared with other types of modern aquaculture production. The last two decades have seen a significant increase in the development of this technology, with increased market acceptance and scale. This chapter provides a brief overview of the history, water quality control processes, new developments and ongoing challenges of RAS.


Author(s):  
Gražina ŽIBIENĖ ◽  
Alvydas ŽIBAS

In this article we analyse catfish rearing conditions in recirculating aquaculture systems (RAS). Based on research, performed by scientists from different countries, we evaluated optimal water parameter values for catfish rearing. African catfish growing process and with it associated factors were researched in JC ,,Šamas“. The amount of dissolved oxygen (on average 4,53 mg/l) in the rearing basin is adequate for the catfish to feel comfortable and for biomass growth. Water temperature only varies slightly – the average water temperature is 23,30C. The ideal pH for rearing fish in RAS is 7.0. Many aquaculture species continue to feel comfortable in pH boundaries of 6.5-8.5. In the samples, taken from catfish rearing system, the pH varied from 5.58 to 6.63. Such acidic water decreases the effectiveness of biological filter, but also decreases the toxic effect of excreted ammonia on the fish. Only a small amount of suspended solid is present in the samples, which means that they are being removed properly. The average amount of ammonia nitrogen in the water basins in analysed catfish rearing systems is 1,171 mg/l. The analysed water samples presented an average nitrite amount of 0,974 mg/l. The recommended amount of nitrites for catfish is less than 0.5 mg/l. The analysed samples presented relatively high nitrate values – from 412 to 495 mg/l. In order to decrease the concentration of accumulated nitrates it is required to change no less than 10% of total system water volume every day. The index of consumed biochemical oxygen corresponds to the requirements for water quality in RAS


Author(s):  
G. Manoj Kumar ◽  
S.E. Gouthem ◽  
A. Srithar ◽  
V. Surya Prakash

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