scholarly journals Iron Supplementation in Experimental Hyperthyroidism: Effects on Oxidative Stress in Skeletal Muscle Tissue

2004 ◽  
Vol 45 (3) ◽  
pp. 413 ◽  
Author(s):  
Hakki Oktay Seymen ◽  
Sabiha Civelek ◽  
Arzu Seven ◽  
Günnur Yiğit ◽  
Hüsrev Hatemi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Iron Supplementation ◽  
Skeletal Muscle Tissue ◽  
Experimental Hyperthyroidism

scholarly journals PO-167 Effects of Hydrogen-rich Water on Rat Skeletal Muscles' Oxidative Damage and Autophagy Induced by Repeated Exhaustive Exercise

2018 ◽  
Vol 1 (4) ◽  
Author(s):  
Zujie Xu ◽  
Hongdi Gu ◽  
Zhenjun Tian
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Protein Expression ◽  
Muscle Tissue ◽  
Skeletal Muscle Tissue ◽  
Exhaustive Exercise ◽  
Ultrastructural Changes ◽  
Sod Activity ◽  
Hydrogen Water ◽  
Mda Content

Objective This study was carried out to detect the effects of hydrogen-water intervention on oxidative stress and cell autophagy in skeletal muscle of rats with repeated exhaustion.  Methods 30 male SD rats in the age of 3 months, weighing 180-210g, were randomly divided into control group (C), repeated exhaustion group (EX), and the repeated exhaustion with hydrogen intervention group (EH), 10 rats in each group. The EX and EH groups were subjected to a four-weeks of repeated-exhaustive exercise. The initial speed of the exercise was 15 m/min, and increasedby 5 m/min every 5 min, the final speed is 35m/min until  exhaustion, 5 d/wk, with a total of 4 wk. In EH group, hydro-water was given to rats 30 mins before exercise. The ultrastructural changes of skeletal muscle were observed by using a transmission electron microscopy. Activity of SDH and CK in serum and SOD activity, MDA content and T-AOC level in skeletal muscle tissue were detected. Western blotting was used to detect the proteins expression of autophagy related proteins in skeletal muscle, mTOR, p-mTOR, LC3B-2 and P53.  Results Compared with the EX group, in the EH group, the ultrastructural damage and mitochondrial swelling were significantly reduced, and the time of exhaustion was significantly prolonged (p<0.05), Serum SDH activity increased significantly (p<0.05), CK activity decreased significantly (p<0.05), and skeletal muscle tissue SOD activity and total antioxidant capacity significantly increased (p<0.05), MDA content decreased significantly (p<0.01), mTOR and p-mTOR protein expression was significantly increased(p<0.05), the LC3B-2 and P53 protein expression was significantly lower (p<0.05). Conclusions Hydrogen water intervention could significantly improve repeatedly exhaustion exercise result in rat skeletal muscle injury, oxidative stress and cell ultrastructure damage excessive autophagy, improving oxidation resistance of the skeletal muscle and exercise endurance.

scholarly journals Innovative approaches to identifying the relationship between the distribution of the concentration of enzymes in the blood serum and skeletal muscle tissue of rats

2019 ◽  
Vol 135 ◽  
pp. 01098
Author(s):  
Pavel Boriskin ◽  
Olga Gulenko ◽  
Anatoliy Deviatkin ◽  
Rufia Karimova ◽  
Olga Pavlova
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Blood Serum ◽  
Muscle Tissue ◽  
Functional Activity ◽  
Active Oxygen Species ◽  
Free Radical Oxidation ◽  
The Body ◽  
Skeletal Muscle Tissue ◽  
Radical Oxidation

One of the reasons for the increase in disability and mortality among the working population is cardiovascular diseases. Long-term studies of the nature of diseases belonging to this group hypertension, renovascular and malignant arterial hypertension, dyslipidemia, diabetic and dilatational cardiomyopathy, atherosclerosis, ischemic heart disease show that the main cause of pathogenesis is the formation of oxidative stress under the influence of internal and external environmental factors. Mismatch between the processes of free-radical oxidation caused by such active oxygen species as superoxide-anion radical, hydroxide-radical radical, lipid radical, hydrogen peroxide, peroxynitrite, chloric acid and the capabilities of the antioxidant system leads to a disruption of the functional activity of cell structures, displacement of homeostatic balance in the body. Despite the rather deep study of cardiovascular system pathologies, data on the functional activity of myocardial cells under destructive effects and oxidative stress are still insufficient.The article presents the study of the interrelation of the concentration distribution of enzymes of the lipidantioxidant peroxidation system in blood serum and skeletal muscle tissue of white non-pedigree rats. By means of Spearman, Gamma and Kendel Tau correlation coefficients it was revealed a reliable presence of a weak force of inverse correlation between the concentration of catalase in blood serum and skeletal muscle (Spearman R = -0.21 at p < 0.011749, Gamma = -0.14 at p < 0.010977; Kendall Tau = -0.14 at p < 0.010977).

scholarly journals Study of superoxiddismutase activity dynamics in rat skeletal muscle tissue under mechanical influence on hemato-ophthalmic barrier

2020 ◽  
Vol 210 ◽  
pp. 06013
Author(s):  
Anatoly Devyatkin ◽  
Olga Pavlova ◽  
Olga Gulenko ◽  
Pavel Boriskin ◽  
Olga Tulaeva
Keyword(s):  
Oxidative Stress ◽  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Mechanical Action ◽  
The Body ◽  
Skeletal Muscle Tissue ◽  
Male Rats ◽  
Rat Skeletal Muscle ◽  
Sod Activity ◽  
Barrier Materials

Hemato-ophthalmic barrier is one of the mechanisms of body resistance. One of the complications of mechanical trauma of the eye and violation of the hemato-ophthalmic barrier is the emergence of oxidative stress on the background of the general inflammatory process. Normally, oxidative stress in the skeletal muscle tissue is not a damaging agent, but when intensified by other factors, it promotes pathological changes in the body. Objective: to study the dynamics of superoxiddismutase (SOD) activity in rat skeletal muscle tissue under oxidative stress caused by mechanical action on the hemato-ophthalmic barrier. Materials and methods: The study was carried out on pedigree matured male rats in the amount of 150 pieces. The activity of SOD in skeletal muscle tissue was studied before the experiment, as well as on the 1st, 3rd, 5th, 7th and 14th day of the experiment using the standard technique of V.S. Gurevich. The obtained digital material was subjected to statistical processing by means of non-parametric statistical analysis. Conclusion: SOD activity in rat skeletal muscle tissue under oxidative stress caused by mechanical action on hemato-ophthalmic barrier is most effectively stabilized in standard therapy of mechanical eye injury with the addition of quercetin in the form of injections.

scholarly journals Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Selva Bilge ◽  
Emre Ergene ◽  
Ebru Talak ◽  
Seyda Gokyer ◽  
Yusuf Osman Donar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Carbonaceous Material ◽  
Hydrothermal Carbonization ◽  
Skeletal Muscle Tissue ◽  
Myotube Formation ◽  
3D Printed

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.

Diffusion of water in skeletal muscle tissue is not influenced by compression in a rat model of deep tissue injury

2010 ◽  
Vol 43 (3) ◽  
pp. 570-575 ◽  
Author(s):  
Bastiaan J. van Nierop ◽  
Anke Stekelenburg ◽  
Sandra Loerakker ◽  
Cees W. Oomens ◽  
Dan Bader ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Tissue ◽  
Rat Model ◽  
Tissue Injury ◽  
Skeletal Muscle Tissue ◽  
Deep Tissue ◽  
Deep Tissue Injury
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