scholarly journals Molecular identification of Trichogramma species from South and South-East Asia and natural Wolbachia infection

2019 ◽  
Vol 28 (2) ◽  
pp. 57-66 ◽  
Author(s):  
Yu-Di Liu ◽  
Mao-Lin Hou ◽  
Kai Song

Trichogramma wasps were collected from the parasitized eggs of lepidopteran pests from 21 sampling sites in East Asia and South-East Asia. Six Trichogramma species were identified based on the molecular identificationmethod using the internal transcribed spacer 2 (ITS2) region of the rDNAof Trichogramma chilonis, T. evanescens, T. ostriniae, T. embryophagum, T. dendrolimi and T. japonicum. The results of molecular identification were confirmed by morphological identification. Additionally, natural populations were screened for the prevalence of Wolbachia. Five out of 21 populations were infected by the same Wolbachia strain, which was identified by using Wolbachia wsp gene and multilocus sequencing approach. The phylogenetic analysis of Wolbachia wsp sequences revealed that the Wolbachia strain was classified in the strain wEvaA in the group of EvA of the supergroup A.

2016 ◽  
Vol 6 (2) ◽  
pp. 882-889
Author(s):  
Ernieenor Faraliana Che Lah

A reliable and rapid taxonomic identification of a mite is the basis for a correct diagnosis of important mite associated allergies as they produce species-specific allergens. A double approach (molecular and morphological) to the taxonomic identification of Aleuroglyphus ovatus was presented. Molecular identification was performed with amplification of the internal transcribed spacer region (ITS2), whilst morphological characters were examined under light microscope. The BLAST results obtained from molecular analysis of A. ovatus was shown to be in concordance with the morphological identification with 97% genetic similarity. Thus, the molecular identification based on the ITS2 region can be applied as a reliable and efficient tool for species identification of Aleuroglyphus and probably any other astigmatid mites. Our findings suggest the need for a broad taxonomic sampling especially from closely related species for an accurate identification of local mites using both DNA sequences and morphology.


2018 ◽  
Vol 3 (3) ◽  

To determine the immunization status of pediatric patients under age of 5 years visiting pediatric department of tertiary care hospitals in South East Asia. The aim of this study was to appreciate the awareness and implementation of vaccination in pediatric patients who came into pediatric outpatient Department with presenting complain other than routine vaccination. we can also know the count of patients who do not complete their vaccination after birth. we can differentiate between vaccinated and unvaccinated patients and incidence of severe disease in both groups. Immunization is a protective process which makes a person resistant to the harmful diseases prevailing in the community, typically by vaccine administration either orally or intravenously. It is proven for controlling and eliminating many threatening diseases from the community. WHO report that licensed vaccines are available for the prevention of many infectious diseases. After the implementation of effective immunization the rate of many infectious diseases have declined in many countries of the world. South-East Asia is far behind in the immunization coverage. An estimated total coverage is 56%-88% for a fully immunized child, which is variable between countries. Also the coverage is highest for BCG and lowest for Polio.


2014 ◽  
Vol 41 (2) ◽  
pp. 230-239 ◽  
Author(s):  
Charles E. Jarvis ◽  
Joanne H. Cooper

It had long been believed that none of the bird, egg or nest specimens that had been in the collection of Sir Hans Sloane at his death in 1753 had survived. However, a specimen of a rhinoceros hornbill, originally in Sloane's hands, was discovered in the Natural History Museum's collections in London in 2003, and three more Sloane hornbill specimens have subsequently come to light. In addition, we report here a most unexpected discovery, that of the head of a woodpecker among the pages of one of Sloane's bound volumes of pressed plants. The context suggests that the head, like its associated plant specimens, was probably collected in south-east Asia about 1698–1699 by Nathanael Maidstone, an East India Company trader, the material reaching Sloane via William Courten after the latter's death in 1702. A detailed description of the head is provided, along with observations on its identity and possible provenance.


Writing from a wide range of historical perspectives, contributors to the anthology shed new light on historical, theoretical and empirical issues pertaining to the documentary film, in order to better comprehend the significant transformations of the form in colonial, late colonial and immediate post-colonial and postcolonial times in South and South-East Asia. In doing so, this anthology addresses an important gap in the global understanding of documentary discourses, practices, uses and styles. Based upon in-depth essays written by international authorities in the field and cutting-edge doctoral projects, this anthology is the first to encompass different periods, national contexts, subject matter and style in order to address important and also relatively little-known issues in colonial documentary film in the South and South-East Asian regions. This anthology is divided into three main thematic sections, each of which crosses national or geographical boundaries. The first section addresses issues of colonialism, late colonialism and independence. The second section looks at the use of the documentary film by missionaries and Christian evangelists, whilst the third explores the relation between documentary film, nationalism and representation.


2017 ◽  
Vol 10 (1) ◽  
pp. 59-73
Author(s):  
Michelle Ann Abate ◽  
Sarah Bradford Fletcher

Since its release in 1963, Maurice Sendak's Where the Wild Things Are has been viewed from a psychological perspective as a literary representation of children's inner emotional struggles. This essay challenges that common critical assessment. We make a case that Sendak's classic picturebook was also influenced by the turbulent era of the 1960s in general and the nation's rapidly escalating military involvement in Vietnam in particular. Our alternative reading of Sendak's text reveals a variety of both visual and verbal elements that recall the conflict in South East Asia and considers the significance of the book's geo-political engagement.


2005 ◽  
Vol 21 (1) ◽  
pp. 75-84
Author(s):  
Yuzuru HAMADA ◽  
Hideyuki OHSAWA ◽  
Shunji GOTO ◽  
Yoshi KAWAMOTO ◽  
Toru OI ◽  
...  

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