36. Sample Processing Recovery Evaluation of Bacillus Anthracis from Environmental Sampling Media

2004 ◽  
Author(s):  
P. Kostle ◽  
M. Pentella ◽  
W. Sanderson
Keyword(s):  
Bacillus Anthracis ◽  
Environmental Sampling ◽  
Sample Processing

scholarly journals Rapid Detection of Bacillus anthracis Bloodstream Infections by Use of a Novel Assay in the GeneXpert System

2017 ◽  
Vol 55 (10) ◽  
pp. 2964-2971 ◽  
Author(s):  
Padmapriya P. Banada ◽  
Srinidhi Deshpande ◽  
Riccardo Russo ◽  
Eric Singleton ◽  
Darshini Shah ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Severe Disease ◽  
Automated System ◽  
Bacterial Isolates ◽  
Sample Processing ◽  
Blood Samples ◽  
Content Type

ABSTRACT Bacillus anthracis is a tier 1 select agent with the potential to quickly cause severe disease. Rapid identification of this pathogen may accelerate treatment and reduce mortality in the event of a bioterrorism attack. We developed a rapid and sensitive assay to detect B. anthracis bacteremia using a system that is suitable for point-of-care testing. A filter-based cartridge that included both sample processing and PCR amplification functions was loaded with all reagents needed for sample processing and multiplex nested PCR. The assay limit of detection (LOD) and dynamic range were determined by spiking B. anthracis DNA into individual PCR mixtures and B. anthracis CFU into human blood. One-milliliter blood samples were added to the filter-based detection cartridge and tested for B. anthracis on a GeneXpert instrument. Assay specificity was determined by testing blood spiked with non-anthrax bacterial isolates or by testing blood samples drawn from patients with concurrent non- B. anthracis bacteremia or nonbacteremic controls. The assay LODs were 5 genome equivalents per reaction and 10 CFU/ml blood for both the B. anthracis Sterne and V1B strains. There was a 6-log 10 dynamic range. Assay specificity was 100% for tests of non- B. anthracis bacterial isolates and patient blood samples. Assay time was less than 90 min. This automated system suitable for point-of-care detection rapidly identifies B. anthracis directly from blood with high sensitivity. This assay might lead to early detection and more rapid therapy in the event of a bioterrorism attack.

scholarly journals Optimization of a sample processing protocol for recovery of Bacillus anthracis spores from soil

2016 ◽  
Vol 130 ◽  
pp. 6-13 ◽  
Author(s):  
Erin E. Silvestri ◽  
David Feldhake ◽  
Dale Griffin ◽  
John Lisle ◽  
Tonya L. Nichols ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Sample Processing ◽  
Bacillus Anthracis Spores

Evaluation of tools for environmental sampling of Bacillus anthracis spores

2015 ◽  
Vol 257 ◽  
pp. 376-378 ◽  
Author(s):  
Yoshihito Fujinami ◽  
Junji Hosokawa-Muto ◽  
Natsuko Mizuno
Keyword(s):  
Bacillus Anthracis ◽  
Environmental Sampling ◽  
Bacillus Anthracis Spores

scholarly journals National Validation Study of a Cellulose Sponge Wipe-Processing Method for Use after Sampling Bacillus anthracis Spores from Surfaces

2011 ◽  
Vol 77 (23) ◽  
pp. 8355-8359 ◽  
Author(s):  
Laura J. Rose ◽  
Lisa Hodges ◽  
Heather O'Connell ◽  
Judith Noble-Wang
Keyword(s):  
Bacillus Anthracis ◽  
Validation Study ◽  
Processing Method ◽  
Environmental Sampling ◽  
Content Type ◽  
Cellulose Sponge ◽  
Response Network ◽  
Percent Recovery ◽  
Bacillus Anthracis Spores ◽  
Sensitivity Specificity

ABSTRACTThis work was initiated to address the gaps identified by Congress regarding validated biothreat environmental sampling and processing methods. Nine Laboratory Response Network-affiliated laboratories participated in a validation study of a cellulose sponge wipe-processing protocol for the recovery, detection, and quantification of viableBacillus anthracisSterne spores from steel surfaces. Steel coupons (645.16 cm2) were inoculated with 1 to 4 log10spores and then sampled with cellulose sponges (Sponge-Stick; 3M, St. Paul, MN). Surrogate dust and background organisms were added to the sponges to mimic environmental conditions. Labs processed the sponges according to the provided protocol. Sensitivity, specificity, and mean percent recovery (%R), between-lab variability, within-lab variability, and total percent coefficient of variation were calculated. The mean %R (standard error) of spores from the surface was 32.4 (4.4), 24.4 (2.8), and 30.1 (2.3) for the 1-, 2-, and 4-log10inoculum levels, respectively. Sensitivities for colony counts were 84.1%, 100%, and 100% for the 1-, 2-, and 4-log10inocula, respectively. These data help to characterize the variability of the processing method and thereby enhance confidence in the interpretation of the results of environmental sampling conducted during aB. anthraciscontamination investigation.

Automated radon monitoring system for continuous environmental sampling

1990 ◽  
Vol 25 (12) ◽  
pp. 1259-1263
Author(s):  
Domenica Paoletti ◽  
Giuseppe Schirripa Spagnolo
Keyword(s):  
Monitoring System ◽  
Environmental Sampling ◽  
Radon Monitoring

Exemplar Abstract for Bacillus anthracis Cohn 1872 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Bacillus Anthracis
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