scholarly journals The Effect of Feeding with Resveratrol on Myogenin and mTOR Levels in Mice

2020 ◽  
pp. 1-6
Author(s):  
Serap Andaç Öztürk ◽  
Gül Kızıltan ◽  
Erkan Yurtçu ◽  
Didem Bacanlı ◽  
Yeşim Korkmaz Kasap ◽  
...  
Keyword(s):  
Body Weight ◽  
Muscle Mass ◽  
Muscle Hypertrophy ◽  
Total Body Weight ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Time Interval ◽  
Study Group ◽  
Total Body ◽  
The Right

Aim: It is reported that resveratrol may induce muscle hypertrophy and myogenesis. Accordingly, resveratrol is promising for the treatment of muscle diseases and ergogenic aid for athletes. In this study it is aimed to observe the effects of resveratrol on myogenin and mTOR levels and muscle mass. Material and Methods: The study is performed on 14 Swiss albino young adult mice at Başkent University Experimental Animal Research Center. The mice are divided randomly into study (n:7) and control (n:7) groups. During consecutive 7 days, 20 mg/kg trans-resveratrol was given to the study group intraperitoneally. After the seventh day administration, the right gastrocnemius muscles are disected, weighted and stored at -80 °C for mTOR and myogenin analysis. Then, myogenin and mTOR levels are determined by enzyme-linked immunosorbent assay (ELISA) method in homogenised muscle mass. Results: According to mean myogenin and mTOR levels, there was no significantly difference between groups (p>0.05). Total body weight and the right gastrocnemius muscle weight of the study group (22.11±3.22 g, 0.09±0.03 g, respectively) at the 7th day was found significantly lower than control group (28.63±3.80 g, 0.12±0.02 g, respectively) (p<0.05). Conclusion: It was observed that resveratrol administration had no effect on myogenin and mTOR levels which is a fore-step for muscle hypertrophy. On the other hand, interestingly total body weight and muscle mass decrease was observed in the study group. The effects of resveratrol on muscle tissue is a relatively less studied topic. However, the authors are suggesting more studies on the resveratrol usage or administration for the effective dose and time interval investigation.

scholarly journals Mesenchymal Stem Cell-Derived Exosomes Protect Muscle Loss by miR-145-5p Activity Targeting Activin A Receptors

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 2169
Author(s):  
Kyung-Ah Cho ◽  
Da-Won Choi ◽  
Yu-Hee Kim ◽  
Jungwoo Kim ◽  
Kyung-Ha Ryu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Body Weight ◽  
Muscle Mass ◽  
Skeletal Muscle Mass ◽  
Total Body Weight ◽  
Activin A ◽  
Dependent Manner ◽  
Muscle Loss ◽  
Total Body

Skeletal muscle mass is decreased under a wide range of pathologic conditions. In particular, chemotherapy is well known for inducing muscle loss and atrophy. Previous studies using tonsil-derived mesenchymal stem cells (T-MSCs) or a T-MSC-conditioned medium showed effective recovery of total body weight in the chemotherapy-preconditioned bone marrow transplantation mouse model. This study investigated whether extracellular vesicles of T-MSCs, such as exosomes, are a key player in the recovery of body weight and skeletal muscle mass in chemotherapy-treated mice. T-MSC exosomes transplantation significantly decreased loss of total body weight and muscle mass in the busulfan-cyclophosphamide conditioning regimen in BALB/c recipient mice containing elevated serum activin A. Additionally, T-MSC exosomes rescued impaired C2C12 cell differentiation in the presence of activin A in vitro. We found that T-MSC exosomes possess abundant miR-145-5p, which targets activin A receptors, ACVR2A, and ACVR1B. Indeed, T-MSC exosomes rescue muscle atrophy both in vivo and in vitro via miR-145-5p dependent manner. These results suggest that T-MSC exosomes have therapeutic potential to maintain or improve skeletal muscle mass in various activin A elevated pathologic conditions.

La fraye d'une population d'éperlans géants, Osmerus eperlanus mordax, au lac Heney, Comté de Gatineau, Québec

1968 ◽  
Vol 25 (9) ◽  
pp. 1813-1830 ◽  
Author(s):  
Roméo-O. Legault ◽  
Claude Delisle
Keyword(s):  
Body Weight ◽  
Total Body Weight ◽  
The Body ◽  
Testis Weight ◽  
Spawning Period ◽  
Weight One ◽  
Total Body ◽  
Set Up ◽  
The Right ◽  
Two Populations

Two sympatric smelt populations inhabit Lake Heney, Gatineau County, Quebec. This study deals only with the population which we call "giant" but a brief discussion on "small smelt — large smelt" theory is included. The data were obtained from 1556 specimens collected with eight gillnets set up under the ice at one locality on Lake Heney, in February, March, and April 1967. The data permitted the determination of the time and site of spawning in the giant population. The time and site of spawning in the "dwarf" population was already known, and we found a marked difference in the spawning behavior and time of spawning of the two populations. The two populations are furthermore reproductively isolated. The sex ratio during the spawning period was closely followed and found to be similar to that of all other freshwater or marine smelts. A DeGiusti dredge, dragged along the bottom below the position of the gillnets immediately after the melting of the ice, permitted the collection of embryonic smelt eggs from a depth of 30–40 ft.The maturity index, relative fecundity, and intergonadal ratio were especially studied. Ovary weight at maturity represents in average one-quarter the total body weight, and testis weight one-fortieth the body weight. A 213-mm female lays an average of 42,460 eggs. The right ovary is 4.5 times smaller than the left one, and the right testis is on the average 3.8 times smaller than the left one.There is a direct relationship between total fecundity and length of mature females. This relation is expressed by the curve log E = 4.54 log L + k.

Proposed corrections in the KERR method on estimating total body weight and calculating muscle mass in athletes

1992 ◽  
Vol 25 (7) ◽  
pp. 702
Author(s):  
F.J. Berral ◽  
A. Escribano ◽  
C.J. Berral ◽  
J.L.Lancho Alonso ◽  
E. DeRose
Keyword(s):  
Body Weight ◽  
Muscle Mass ◽  
Total Body Weight ◽  
Total Body

scholarly journals THE INFLUENCE OF ANEMIA ON PHAGOCYTIC FUNCTIONS AND RESISTANCE TO INFECTION IN MICE

Blood ◽  
1947 ◽  
Vol 2 (Special_Issue_Number_1) ◽  
pp. 117-124 ◽  
Author(s):  
L. JOE BERRY ◽  
EVELYN C. HALLER
Keyword(s):  
Body Weight ◽  
Survival Rate ◽  
Blood Loss ◽  
Salmonella Typhimurium ◽  
Total Body Weight ◽  
Control Group ◽  
Phagocytic Function ◽  
Progressive Development ◽  
Resistance To Infection ◽  
Total Body

Abstract The increase in phagocytic function of neutrophiles of mice was measured during the progressive development of anemia due to blood loss. The animals were bled from the tail, 2 per cent of total body weight being removed twice weekly for nine bleedings. Phagocytosis was 161 per cent of normal at the end of this period, and the erythrocyte totals and hemoglobin values were approximately two thirds of normal. The anemic mice were infected intraperitoneally with a suspension of Salmonella typhimurium and their survival rate was compared with normal mice similarly infected. Twenty-eight of 63 anemic mice survived and 14 of 63 normal mice survived. In a second experiment, mice were subjected to bleeding every third day for nine bleedings, and were then infected along with a control group. One half of the anemic animals were bled for three additional bleedings after the infection. Two of 48 control animals survived, 5 of 47 anemic mice, additionally bled, survived, and 19 of 47 anemic mice, not bled after infection, survived. This suggests that the increase in phagocytosis that accompanies anemia may be, at least in part, responsible for the increased resistance to infection.

scholarly journals Effects of Chronic Use of Methylphenidate on Spermatogenesis and Sexual Hormones in Adult Male Rats

2020 ◽  
Vol 12 (2) ◽  
Author(s):  
Alireza Akhavan Rezayat ◽  
Amir Abbas Asadpour ◽  
Samaneh Boroumand Noughabi ◽  
Hassan Ahmadnia ◽  
Hamid Mohseni ◽  
...  
Keyword(s):  
Body Weight ◽  
Sperm Count ◽  
Total Body Weight ◽  
Male Rats ◽  
Control Group ◽  
Testis Weight ◽  
Control Groups ◽  
Major Health Problem ◽  
Total Body ◽  
And Control

Background: The growing prevalence of Attention Deficit Hyperactivity Disorder (ADHD) and the non-medical use of Methylphenidate (MPH) among the youth have lead male infertility to be a major health problem. Objectives: The present study was conducted to investigate the impacts of MPH administration on different aspects of productivity, including total body weight, testis weight, spermatogenesis, sperm motility, histopathology changes, and sex hormone serum levels in male rats. Methods: This study was performed with 54 eight-week-old male rats divided into one control and two experimental groups. The experimental groups were gavaged with 2 and 10 mg/kg methylphenidate daily while the control group was gavaged with normal saline (at the same dosage). After 60 days, rats were subjected to blood sampling and bilateral orchidoepididymectomy under anesthesia. Spermogram, histological, and hormonal evaluations were performed on the samples. Testes weight and total body weight were also recorded. Results: The results revealed significant differences between the MPH and experimental groups in terms of hormonal, spermographic, and histopathologic features, as well as weight. Luteinizing hormone and testosterone levels, sperm count and motility, Leydig cell hyperplasia, spermatogenesis, congestion and necrosis levels, total body weight, and testis weight were significantly different between the experimental and control groups. However, no difference was observed between the experimental and control groups concerning follicle-stimulating hormone, maturation arrest, and edema levels. Conclusions: Based on the findings, MPH exposure exerts a significant effect on the testis and total body weight, as well as hormonal, spermatographic, and histopathologic characteristics. Accordingly, the present study provided an insight into the negative impression of MPH on sexual parameters.

Effects of some probiotics during its use as a growth promoter in calves

2019 ◽  
Vol 20 (1) ◽  
pp. 62-66
Author(s):  
Magdy Amer
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Total Body Weight ◽  
Treated Group ◽  
The Body ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Growth Promoter ◽  
Probiotic Strains ◽  
Total Body

This study was performed on to evaluate the effect of some probiotic strains (Pediococcus acidophilus and Pedicoccus pentosaceus) on performance, blood picture and some biochemical parameters in serum of treated calves (10 weaned calves 90-115 kg). Calves were classified into 2 equal groups (each of 5 calves). The 1st group was served as the control group while the second group was given Maxboost® (20gm/head/day) in drinking water (after fermentation for 2-3 hours) for five months. Calves were weighed monthly to calculate the body weight gain till the end of the experiment whole blood weight and serum were collected from both groups at 1st, 2nd, 3rd, 4th and 5th month. Results revealed a significant increase in total body weight, body gain, total erythrocytes count, hemoglobin content, total leucocytes count, total protein and mean corpuscular hemoglobin. On the other hand, there were a significant decrease in serum aspartate transaminase (AST), serum alanine transaminase (ALT) and urea in pediococcus treated group compared to the control one.

Expression and Significance of Th17 Cells and Related Factors in Patients with Autoimmune Hepatitis

2019 ◽  
Vol 22 (4) ◽  
pp. 232-237 ◽  
Author(s):  
Jihong An
Keyword(s):  
Autoimmune Hepatitis ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Treg Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Serum Total Bilirubin ◽  
Study Group ◽  
Related Factors ◽  
Tnf Α

Objective: This study aims to investigate the expression and clinical significance of Th17 cells and related factors in peripheral blood of patients with Autoimmune Hepatitis (AIH). Methods: A retrospective selection of 100 patients with AIH were included as a study group, and 100 healthy volunteers in the outpatient clinic were selected as the control group. The levels of IL- 17, IL-6, IL-21 and TNF-α in peripheral blood of all subjects were detected by enzyme-linked immunosorbent assay and the frequency of Th17 cells and Treg cells was detected by flow cytometry. Results: Results showed that the study group had higher levels of serum total bilirubin (TBil), alkaline phosphatase (ALP), γ -glutamyltranspeptidase (γ-GT), immunoglobulin G (IgG), immunoglobulin M (IgM), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) than the control group, as well as higher levels of IL-17, IL-6, IL-21 and TNF-α in serum. The frequency of Th17 cells in peripheral blood was higher in the study group, while the frequency of Treg cells was lower. Also, serum IL-17, TNF-α levels and Th17 cells frequency were positively correlated with ALT and AST, whereas Treg cells frequency were negatively correlated with ALT and AST levels. Conclusion: Our finding demonstrates that Th17 cell frequency and their related factors IL-17 and TNF-α, are associated with liver damage, which might be used to monitor AIH disease severity.

scholarly journals Lean body weight versus total body weight to calculate the iodinated contrast media volume in abdominal CT: a randomised controlled trial

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Moreno Zanardo ◽  
Fabio Martino Doniselli ◽  
Anastassia Esseridou ◽  
Massimiliano Agrò ◽  
Nicol Antonina Rita Panarisi ◽  
...  
Keyword(s):  
Body Weight ◽  
Contrast Media ◽  
Controlled Trial ◽  
Total Body Weight ◽  
Lean Body Weight ◽  
Iodinated Contrast Media ◽  
Abdominal Ct ◽  
Iodinated Contrast ◽  
Significant Difference ◽  
Total Body

Abstract Objectives Iodinated contrast media (ICM) could be more appropriately dosed on patient lean body weight (LBW) than on total body weight (TBW). Methods After Ethics Committee approval, trial registration NCT03384979, patients aged ≥ 18 years scheduled for multiphasic abdominal CT were randomised for ICM dose to LBW group (0.63 gI/kg of LBW) or TBW group (0.44 gI/kg of TBW). Abdominal 64-row CT was performed using 120 kVp, 100–200 mAs, rotation time 0.5 s, pitch 1, Iopamidol (370 mgI/mL), and flow rate 3 mL/s. Levene, Mann–Whitney U, and χ2 tests were used. The primary endpoint was liver contrast enhancement (LCE). Results Of 335 enrolled patients, 17 were screening failures; 44 dropped out after randomisation; 274 patients were analysed (133 LBW group, 141 TBW group). The median age of LBW group (66 years) was slightly lower than that of TBW group (70 years). Although the median ICM-injected volume was comparable between groups, its variability was larger in the former (interquartile range 27 mL versus 21 mL, p = 0.01). The same was for unenhanced liver density (IQR 10 versus 7 HU) (p = 0.02). Median LCE was 40 (35–46) HU in the LBW group and 40 (35–44) HU in the TBW group, without significant difference for median (p = 0.41) and variability (p = 0.23). Suboptimal LCE (< 40 HU) was found in 64/133 (48%) patients in the LBW group and 69/141 (49%) in the TBW group, but no examination needed repeating. Conclusions The calculation of the ICM volume to be administered for abdominal CT based on the LBW does not imply a more consistent LCE.

scholarly journals Frequency-Following Response (FFR) in Military Pilots

2020 ◽  
Author(s):  
Graziela Maria Martins-Moreira ◽  
Alessandra Spada Durante
Keyword(s):  
Pure Tone Audiometry ◽  
Control Group ◽  
Study Group ◽  
Aircraft Accidents ◽  
Frequency Following Response ◽  
Significant Difference ◽  
Qualitative Variables ◽  
Chi Squared ◽  
The Right ◽  
Military Pilots

Abstract Introduction Good hearing in pilots, including central auditory skills, is critical for flight safety and the prevention of aircraft accidents. Pure tone audiometry alone may not be enough to assess hearing in the members of this population who, in addition to high noise levels, routinely face speech recognition tasks in non-ideal conditions. Objective To characterize the frequency-following response (FFR) of a group of military pilots compared with a control group. Methods Twenty military pilots in the Study Group and 20 non-pilot military personnel, not exposed to noise in their work, in the Control Group, all with normal hearing, aged between 30 and 40 years old, completed a questionnaire to assess their hearing habits, and their FFRs were measured with a /da/ syllable (duration 40 milliseconds, speed 10.9/s), at 80 dB NA in the right ear. All procedures were approved by the ethical committee of the institution. Statistical analysis was performed using the t-Student or Mann-Whitney tests for quantitative variables, and the Fisher or chi-squared tests for qualitative variables, and a value of p < 0.05 was considered to be statistically significant. Results There was no significant difference between the groups regarding auditory habits. In the FFR, wave amplitudes A (p = 0.01) and C (p = 0.04) were significantly lower in the Study Group. Conclusion Working as a military pilot can be a crucial factor in determining an individual's typical FFR pattern, demonstrated in the present study by statistically significant reductions in the amplitudes of the A and C waves.

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