Botanical, genetic characteristics and preliminary screening of the phytochemical constituents of Hydnophytum formicarum Jack. in Phu Quoc forest, Vietnam

MedPharmRes ◽  
2019 ◽  
Vol 3 (2) ◽  
pp. 8-14
Author(s):  
Nguyen Ngoc Trac Mai ◽  
Thi Dep Truong ◽  
Thi Van Anh Tran ◽  
Thi Hong Tuoi Do
Keyword(s):  
Folk Medicine ◽  
Raw Material ◽  
Total Phenolic ◽  
Genetic Characteristics ◽  
Preliminary Screening ◽  
Phenolic Contents ◽  
Phytochemical Constituents ◽  
Reducing Substances ◽  
Sanger Method ◽  
Rbcl Sequences

In Vietnamese folk medicine, Bi ky nam (H. formicarum) tuber has been widely used to treat rheumatism, liver and intestinal diseases. This work aimed to study botanical, genetic characteristics and screening of the phytochemical constituents of wild H. formicarum of Phu Quoc Island, Vietnam. Anatomical characteristics of the plant material were described. Fresh leaves were used to analyze DNA barcodes based on rbcL region amplified by PCR. Sequences of DNA products were identified by Sanger method and BioEdit 7.0.5 software, then compared to the control rbcL sequences published in GenBank by BLAST. The tuber powder was studied for pharmacognostic parameters, preliminary phytochemical screening and total phenolic contents by Folin-Ciocalteu method. Results showed that the similarity between the rbcL sequences of H. formicarum leaves collected in Phu Quoc and the control one published in Genbank was 99%. Moisture content, total ash value and acid insoluble ash value of dried tuber powder were 11.06%, 9.60% and 0.70%, respectively. Raw material contained carotenoids, triterpenoids, flavonoids, phenolics, tannins, saponins, reducing substances and amino acids. Total phenolics content was about 58.847 mg pyrrogallol equivalent/g dried powder weight. Our results provided information about botanical, genetic and preliminary phytochemical characteristics of H. formicarum growing on Phu Quoc Island. This could be useful for the authentication of H. formicarum as a medicinal material.

scholarly journals Total Phenolic Contents and Antioxidant Properties of Algerian Arbutus unedo L. Extracts

2020 ◽  
Vol 10 (3-s) ◽  
pp. 159-168
Author(s):  
Saliha Laouicha ◽  
Abderrahmane Senator ◽  
Abdallah Kherbache ◽  
Hamama Bouriche
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Arbutus Unedo ◽  
Phenolic Contents ◽  
Antioxidant Agents ◽  
Ic50 Values

Arbutus unedo L. is a plant widely distributed in the Mediterranean basin and North Africa, frequently used in traditional folk medicine to treat diabetes and arterial hypertension. This study aims to evaluate the phenolic composition and antioxidant activity of ethyl acetate (EA.E) and n-butanolic (But.E) extracts prepared from leaves of Arbutus unedo. Total phenolic and flavonoids contents were determined spectrophotometrically. The antioxidant activity was evaluated using DPPH•, OH•, H2O2, O•-2, ß-carotene bleaching and AAPH-induced erythrocyte oxidative hemolysis assays. The phytochemical analysis showed the presence of polyphenols and flavonoids in both extracts. The high amount was observed in EA.E which exerted the stronger antioxidant effect, with IC50 values of 3.43 μg/mL, 323.45 µg/mL, 38.40 µg/mL and 11.56 µg/mL, in DPPH•, OH•, O•-2 and H2O2 tests, respectively. Both extracts inhibited β-carotene bleaching, but EA.E is always more potent (92%) than But.E (85%). Furthermore, the EA.E showed the highest protective effect on erythrocyte hemolysis induced by AAPH, with half time hemolysis (HT50) of 122.02 min at 40 µg/mL. Taken together, this study showed that Arbutus unedo leaf extracts possess strong antioxidant potential, which may be attributed to the presence of a high amount of polyphenolic constituents. So, this plant might be exploited as a potential source of natural antioxidant agents for pharmaceutical and food applications. Keywords: oxidative stress, antioxidant, phenolic compounds, Arbutus unedo.

scholarly journals Biological Value of Centaurea damascena: Minireview

2019 ◽  
Vol 5 (2) ◽  
pp. 99-106
Author(s):  
Mohammad Jaafreh ◽  
Haitham Qaralleh ◽  
Muhamad Al-limoun
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Antibacterial Activities ◽  
Essential Oil Composition ◽  
Total Phenolic ◽  
Oil Composition ◽  
Phenolic Contents ◽  
The Family

The family Asteraceae include large number of Centaurea species which have been applied in folk  medicine. One of the family Asteraceae members is the Centaurea damascena which authentically been tested for its antibacterial activity. The aim of the study was to discuss antibacterial activities of essential oil composition and methanolic extract of the same plant aerial part leaves.  Thirty-seven components were characterized with 86% of oxygenated terpenes. The composition was dominated by 11.45% Fokienol, 8.8% thymol, 8.21% Alpha Terpineol, 7.24% Chrysanthemumic acid, 7.13% Terpinen-4-ol and 6.59% Borneol with a high degree of polymorphism in the occurrence of these compounds as compared with the different species of centaurea.. Free radical scavenging capacity of the C. damascna methanol extract was calculated by DPPH and FRAP test. DPPH radicals were scavenged with an IC50 value of 17.08 µg /ml. Antioxidant capacities obtained by the FRAP was 51.9 and expressed in mg Trolox g-1 dry weight. The total phenolic compounds of the methanol extracts of aerial parts, as estimated by Folin–Ciocalteu reagent method, was about 460 mg GAE/ g. The phenolic contents in the extracts highly correlate with their antioxidant activity, (R2 = 0.976) confirming that the antioxidant activity of this plant extracts is considerably phenolic contents-dependent. 

scholarly journals Phytochemical assessment of Iphiona aucheri (Bioss.) Anderb. and its cytotoxic, antioxidant and antidiabetic activities

2020 ◽  
Vol 49 (4) ◽  
pp. 913-918
Author(s):  
Muhammad Abdur Rehman Shah ◽  
Rahmat Ali Khan ◽  
Mushtaq Ahmed
Keyword(s):  
Brine Shrimp ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Aqueous Fraction ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Total Antioxidant ◽  
Phytochemical Constituents

The presence of phytochemical constituents and estimation of total phenolic contents in Iphiona aucheri (Bioss.) Anderb. stem and assessment of their cytotoxic, total antioxidant and anti-diabetic activity were investigated. All fractions were assessed for phytochemicals, cytotoxic activity, total phenolic contents, antioxidant and anti-diabetic characteristics. Saponins, glycosides, protein and amino acids, carbohydrates and flavonoids were found in aqueous fraction and methanolic extract while they were absent in n-hexane fraction except glycosides and protein. The crude methanolic extract (70.3 ± 1.9%) revealed highest brine shrimp mortalities. Except n-hexane fraction others indicated considerable antioxidant activities via DPPH, ABTS•+ and H2O2 assays. Crude methanolic extract expressed higher inhibition of α-amylase (60.71 ± 0.89%) than glucophage (54.92 ± 0.56%). Non significant correlation of total phenolic contents with percentage antioxidant and anti-diabetic activities of crude methanolic extract and its various fractions was observed in all cases.

scholarly journals Bioactive content and antioxidant capacity of Cape gooseberry fruit

2012 ◽  
Vol 7 (4) ◽  
pp. 672-679 ◽  
Author(s):  
Otakar Rop ◽  
Jiri Mlcek ◽  
Tunde Jurikova ◽  
Magdalena Valsikova
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Raw Materials ◽  
Folk Medicine ◽  
Fresh Fruit ◽  
Human Nutrition ◽  
Raw Material ◽  
Total Phenolic ◽  
Methanolic Extracts ◽  
Cape Gooseberry

AbstractAt present, Cape gooseberry (Physalis peruviana) fruit is one of the less used raw materials of plant origin, which can be used for human nutrition. This fruit, as well as alimentary products made of it, were used by healers in folk medicine in the distant past. The aim of this study was to monitor and evaluate the antioxidant capacity of fresh fruit of three Cape gooseberry cultivars ‘Giant’, ‘Golden berry’ and ‘Inka’. Antioxidant capacity was also tested, on the basis of the scavenging effect of reactive oxygen species (ROS) and lipid peroxidation of methanolic extracts made of fresh fruit. These results were further extended and supplemented with determinates of the vitamin C and total phenolic contents. These analyses were made for three consecutive years. The highest values of antioxidant capacity were observed in the ‘Inka’ cultivar (9.31 grams of ascorbic acid equivalents kg−1 of fresh mass). In this cultivar, the obtained results were corroborated also in ROS and the contents of vitamin C and total phenolics. Due to a high antioxidant capacity of this fruit species, the results presented should increase its popularity above all as a promising raw material, which can be used for human nutrition.

scholarly journals Comparison of antioxidant activity of extracts of hop leaves harvested in different years

2019 ◽  
Vol 65 (3) ◽  
pp. 1-9 ◽  
Author(s):  
Anna Muzykiewicz ◽  
Anna Nowak ◽  
Joanna Zielonka-Brzezicka ◽  
Katarzyna Florkowska ◽  
Wiktoria Duchnik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Folk Medicine ◽  
Raw Material ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Methods Of Evaluation ◽  
Health Promoting ◽  
Common Plant

Summary Introduction: Hop (Humulus lupulus L.) is a common plant in Europe, with many beneficial health effects. In addition to the use in brewing, hops are a valuable source of active substances used in conventional and folk medicine, such as humulones and lupulones, as well as antioxidants, including phenolic compounds. Objective: The aim of the study was to evaluate and compare the antioxidant activity of alcoholic extracts of fresh hop leaves collected in 2017 and 2018. Material and methods: The raw material consisting of fresh hop leaves was extracted using ultrasound-assisted extraction. Methyl, ethyl and isopropyl alcohol at three concentrations were used as extractants. The antioxidant activity of extracts was determined using DPPH and FRAP methods. Total phenolic content was evaluated using the Folin-Ciocalteu technique. Results: All the extracts showed antioxidant potential as well as the phenolic content. Regardless of the harvesting year and methods of evaluation, the highest antioxidant activity and the total polyphenol content were observed for extracts prepared in undiluted methanol, obtained during one hour lasting extraction. Conclusion: The results of the studies have suggested that hop leaves can be a potential source of health-promoting antioxidants.

scholarly journals Phenolic Content, Antioxidant Activity, 15-Lipoxygenase and Lipid Peroxidase Inhibitory Effects of two Medicinal Plants from Burkina Faso: Acacia macrostachya Reich. Ex Benth (Mimosaceae) and Lepidagathis anobrya NEES (Acanthaceae)

2020 ◽  
pp. 18-25
Author(s):  
Adjaratou C. Coulibaly ◽  
W. L. M. Esther B. Kabré ◽  
Mariam N. Traoré ◽  
Tata K. Traoré ◽  
Nouhoun Nignan ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Burkina Faso ◽  
Phenolic Content ◽  
Inflammatory Diseases ◽  
Folk Medicine ◽  
Reducing Power ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Lipoxygenase Inhibition

Acacia macrostachya (Mimosaceae) and Lepidagathis anobrya (Acanthaceae) are two medicinal plants used in Burkina Faso folk medicine for the treatment of inflammatory diseases. The purpose of this study was to assess the phenolic content, the antioxidant, lipoxygenase and lipid peroxidaseinhibitory effects of Acacia macrostachya and Lepidagathis anobrya.The experimental results revealed that Acacia mascrostachya and Lepidagathis anobrya have respective total phenolic contents  varies from 240.13 ± 0.44 and 30.88 ± 0.30 mg GAE / 100 mg extract; and total flavonoids contents of 2.30 ± 0.002 and 4.24 ± 0.28 QE / 100 mg of extract. The two plants demonstrated anti-free radicals (ABTS) capacities of 0.06 and 0.14 TEAC while the FRAP reducing power of 2.24 ± 0.08 and 4.43 ± 0.12 mmol AAE per 100 mg respectively. Acacia macrostachya and Lepidagathis anobrya showed significant inhibitory effect on lipid oxidation with the inhibition percentage values of 55.45 ± 1.48 and 66.36 ± 0.65 respectively. In the 15-lipoxygenase inhibition, Acacia macrostachya demonstrated very important inhibitory effect with the IC50 value of 1.32 ± 0.16 while the effect of Lepidagathis anobrya is very moderate.The results of this work demonstrated the interest of Acacia macrostachya and Lepidagathis anobrya in the management of inflammatory diseases.

scholarly journals Pharmacognostical and phytochemical screening of the root of Martynia annua Linn

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Dr Rahul Kumar Gupta ◽  
◽  
Dr Renu Bharat Rathi ◽  
Keyword(s):  
Folk Medicine ◽  
Herbal Medicines ◽  
Madhya Pradesh ◽  
Raw Material ◽  
World Health ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Herbal Drugs ◽  
Phytochemical Screening ◽  
Hydroalcoholic Extract

Background : for acceptance of Ayurveda at global level there is needed to standardize herbal drugs according to modern techniques. For the standardization of herbal medicines it is essential to establish its identify, purity, quality, safety, and efficacy. This study reports on standardization of Martina annua linn root. In tribal pockets of satpura plateau in Madhya Pradesh. Root paste of Martina annua is used in folk medicine to treat cancer and rheumatism. Objective: This study aimed to indentify anthers herb and establishing pharmacognostical, Physiochemical and phytochemical standard for the Martina annua linn. Root. Material and Methods: Plant roots was collected from Govt. Ayurved college campus, Gwalior, Madhya Pradesh and evaluated for pharmacognostical, Physiochemical and phytochemical parameter using guidelines of the world health organization and pharmacopoeial laboratory for Indian medicine for quality control of herbal drugs. Results: Chemical evaluation shown presence of alkaloids, glycosides, flavonoids, steroids and tannins. The microscopic characters have shown presence of Epiblema, Periderm, Cortex, Phloem and Madullary Rays, and Xylem vessels. Microscopy analysis of the powder included the parenchyma cells, fibers and vessel. In results, it was found that the root containing various phytochemicals were present in its Aqueous extract, Hydroalcoholic extract and Alcoholic extract. Hydroalcoholic extract of Martynia annua root had the highest of total phenolic content (364.15 mg GAE /100gm) and highest total flavonoid content (139.84 mg QE /100gm). Conclusions: Pharmacognostical and preliminary phytochemical screening of Martynia annua roots will be useful in order to authenticate, standardize and avoid any adulteration in the raw material and will be helpful in the development of a monograph.

scholarly journals Antioxidant activity, volatile compounds and fatty acid compositions of Cephalaria syriaca seeds obtained from different regions in Turkey

2020 ◽  
Vol 71 (4) ◽  
pp. 379
Author(s):  
C. Kavak ◽  
A. Baştürk
Keyword(s):  
Antioxidant Activity ◽  
Fatty Acid ◽  
Volatile Compounds ◽  
Food Additives ◽  
Natural Antioxidants ◽  
Tocopherol Content ◽  
Raw Material ◽  
Oil Yield ◽  
Total Phenolic ◽  
Phenolic Contents

Crude oil yield, fatty acid composition, volatile compounds, antioxidant activity and some characteristics of Cephalaria syriaca seeds collected from different locations in Turkey were studied. Antioxidant capacity was determined by DDPH and ABTS tests and the results were in the range of 18.8-67.3% and 0.0-41.8 mmol Trolox eq g-1 DW, respectively; while total phenolic contents were between 4339-11907 mg GAE kg-1. The average α-tocopherol content was found to be in the range of 54-467 mg kg-1. Oil yield was between 11.2-24.0%. Oleic and linoleic acids were the predominant fatty acids. A total of 30 different volatile compounds were identified in the samples, mostly consisting of alcohols and aldehydes. The results of this study showed that Cephalaria syriaca seeds can be considered as alternative raw material in the production of edible oil, and can be used as a source of natural antioxidants and food additives.

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antitumor Activity ◽  
Traditional Method ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>

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