scholarly journals The enzyme activity dynamic relationship with the content of structural polypeptides of enterocyte membranes in cattle fetal

2021 ◽  
Vol 4 (2) ◽  
pp. 3-6
Author(s):  
D. M. Masiuk
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Protein Content ◽  
Apical Membrane ◽  
Basolateral Membrane ◽  
Molecular Weights ◽  
Dynamic Relationship ◽  
Molecular Masses ◽  
Alkaline Activity ◽  
The Relationship

The article performs new data on the relationship between the hydrolytic and transport enzyme activity at different poles of the enterocytes plasmolemma of cow's fetal large intestine with the content of individual fractions of polypeptides. An expressive direct dependence of enzyme activity dynamics on the apical and basolateral membranes of enterocytes containing low molecular weight proteins and an inverse relationship with the concentration of proteins with medium and large molecular weights has been proved. It was found that the alkaline activity of the phosphatase and γ-glutamyltransferase on the apical domain of enterocyte plasmolemma is directly related to the proteins content with molecular masses of 9.6–14.2 kD, 21 kD, 22.5 kD, 26 kD, 33 kD, 35 kD, 170–185 kD, and 205 kD (P ≤ 0.05–0.001). Gamma-glutamyltransferase activity is straightly related to protein quantity with molecular weights of 15.5 kDa and 39 kD (P ≤ 0.05). In contrast, alkaline phosphatase and GGT activity have inverse correlations with the content of polypeptides with molecular masses of 46 kD, 63 kD, and 250 kD in the apical membrane of enterocytes (P ≤ 0.01–0.001). The lactase activity in the cattle enterocytes apical membrane during the test period has significant direct correlations only with the amount of the polypeptide of polypeptides with molecular weights of 31 kD, 39 kD, and 100 kD (P ≤ 0.05–0.01) and inverse relationships containing proteins with molecular masses of 46 kD and 120 kD (P ≤ 0.05). A linear dependence of the different ATPase activity of the apical membrane of red blood cells containing proteins with molecular weights of 9.6–14.2 kD, 15.5 kD, 21 kD, 22.5 kD, 33 kD, 35 kD, 39 kD, and 205 kD (P ≤ 0.05–0.001) was observed. Alkaline phosphatase activity in the apical membrane of enterocytes is only directly related to the number of proteins with molecular weights of 17 kD and 24 kD (P ≤ 0.001) in this domain. It inversely depends on the content of proteins with molecular masses of 9.6–14.2 kD and 52 kD (P ≤ 0.001). G-glutamyltransferase activity is inversely related to protein content with molecular weights of 43 kD, 52 kD, 66 kD, 87 kD, and 100 kD and 155 kD (P ≤ 0.001). The Ca2+, Mg2+-ATPase of the basolateral membrane activity of enterocytes is directly related to the protein amount with molecular weights of 26 kD (P ≤ 0.01), Mg2+-ATPase and Mg2+-ATPase with protein content with the molecular value of 100 kD (P ≤ 0.05).

scholarly journals The morphological changes relationship in the small intestine mucous membrane with the dynamics of the enterocyte’s membranes polypeptide composition in cattle during the fetal period of their ontogenesis

2021 ◽  
Vol 9 (1) ◽  
pp. 52-56
Author(s):  
D. M. Masiuk
Keyword(s):  
Molecular Weight ◽  
Mucous Membrane ◽  
Apical Membrane ◽  
Morphological Changes ◽  
Basolateral Membrane ◽  
Intestinal Wall ◽  
Current Data ◽  
Polypeptide Composition ◽  
Fetal Period ◽  
Molecular Weights

The article presents current data on the relationship of morphological changes in the jejenum intestine mucous membrane with the dynamics of the polypeptide composition in the cattle enterocytes membranes during the fetal period of ontogenesis. The study was carried out on 80 fetuses of Holstein cattle, aged 2–9 months, with a body weight of 0.6–39 kg. It was found that during the fetal period of ontogenesis, structural transformations took place in the jejunal mucous membrane of cattle, characterized by intense morphofunctional changes in its structural components. The polypeptide composition of the jejunal enterocytes plasma membrane in cattle during the fetal period of ontogenesis was determined, in particular, in the apical and basolateral membranes, respectively, 27 and 25 protein fractions with a molecular weight of 9.6–14.2 kDa to 300 kDa were found, respectively. With the help of the correlation analysis for the received data, reliable interconnections of morphological changes in the jejunal mucous layer with the dynamics of the polypeptide composition of the cattle enterocyte’s membranes during the fetal period of ontogenesis were obtained. It has been proven that the thickness of the intestinal wall with villi and the mucous membrane with villi is directly related to the content of proteins with a molecular weight of 250 kDa and 155 kDa on the apical domain of enterocytes (P ≤ 0.01–0.001). The height of the villi is associated only with the content of polypeptides with a molecular weight of 250 kDa and 155 kDa (P ≤ 0.05–001) in the apical membrane and is inversely related to the content of polypeptides with a molecular weight of 9.6–14.2 kDa, 21 kDa, 22.5 kDa, 26 kDa, 33 kDa, 35 kDa, 170–185 kDa (P ≤ 0.01–0.001). The width of the villi is related to the content on the apical membrane of proteins with a molecular weight of 170–185 kDa and 21 kDa (P ≤ 0.05–0.01). The thickness of the intestinal wall with villi and mucous membrane with villi is associated with the content of proteins with molecular weights 155 kDa, 100 kDa, 87 kDa, 66 kDa, 52 kDa) and 43 kDa (P ≤ 0.05–0.001) on the basolateral domain of enterocytes and inversely dependent on the content of polypeptides with molecular weights of 19 kDa, 21 kDa and 31 kDa (P ≤ 0.05). The height of enterocyte villi is directly related to the content of polypeptides with a molecular weight of 155 kDa and 52 kDa (P ≤ 0,05–0,01) in the basolateral membrane and is inversely dependent on the content of polypeptides with a molecular weight of 24 kDa, 22.5 kDa and 17 kDa (P ≤ 0.05–0.001). The width of the villi of enterocytes is reliably inversely related only to the content of proteins with a molecular weight of 155 kDa on the basolateral membrane (P ≤ 0.05).

Blood pyruvate carboxylase (EC 6.4.1.1) activity as a criterion of biotin status in chickens and turkeys

1978 ◽  
Vol 39 (3) ◽  
pp. 547-556 ◽  
Author(s):  
C. C. Whitehead ◽  
D. W. Bannister
Keyword(s):  
Enzyme Activity ◽  
Protein Content ◽  
Pyruvate Carboxylase ◽  
Growth Response ◽  
Maximum Activity ◽  
Maximal Growth ◽  
Blood Pyruvate ◽  
New Criterion ◽  
The Relationship

1. Blood pyruvate carboxylase (pyruvate-CO2 ligase (ADP-forming); EC 6.4.1.1; PC) activities in young chickens and turkeys given low-biotin diets supplemented with biotin at graded levels were studied in three experiments.2. In both species PC activity was related positively to the supplemental biotin level. The relationship was sigmoid and maximum activity was attained with supplemental levels above those required to give maximal growth response.3. Enzyme activity decreased between 2 and 4 weeks of age but remained almost constant thereafter.4. Activity in chicks was not affected by alterations in the fat or protein content of the diet.5. Changing poults from high to low and from low to high supplemental biotin levels resulted in reversals in the levels of enzyme activity.6. It is concluded that blood PC activity is a promising new criterion for assessing the biotin status of young chickens and turkeys.

scholarly journals DISTRIBUTION OF LEUCYL NAPHTHYLAMIDASE AND ALKALINE PHOSPHATASE ON THE VILLI OF THE CHICK DUODENUM

1970 ◽  
Vol 18 (6) ◽  
pp. 416-423 ◽  
Author(s):  
ROBERT D. GREY ◽  
THOMAS S. LECOUNT
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Protein Content ◽  
Age Groups ◽  
Phenyl Phosphate ◽  
Serial Samples

Serial samples from successive levels of the villi and crypts were collected by sectioning a flattened piece of frozen duodenum parallel to the gut wall. Hatched chicks of 1 and 4 weeks of age were studied. Groups of sections were pooled for assay of enzyme activity and protein content. In both age groups, leucyl naphthylamidase was present in the crypts and highest at the bases of the villi while alkaline phosphatase (EC 3.1.3.1) was low or absent in the crypts and highest at the villi tips. Alkaline phosphatase at all levels of the villi hydrolyzed phenyl phosphate faster than β-glycerophosphate; the ratio of activities on these substrates was uniform along the length of the villi, but differed between age groups.

scholarly journals Changes in the structural components of the mucous membrane and plasma proteins of small intestine enterocytes in fetuses of cattle

2021 ◽  
Vol 23 (102) ◽  
pp. 24-28
Author(s):  
D. M. Masiuk
Keyword(s):  
Alkaline Phosphatase ◽  
Mucous Membrane ◽  
Large Intestine ◽  
Plasma Proteins ◽  
Apical Membrane ◽  
Morphological Changes ◽  
Basolateral Membrane ◽  
Intestinal Wall ◽  
Morphometric Parameters ◽  
Enzymatic Systems

The interrelationships of morphological changes of the mucosa of the large intestine with the dynamics of enzyme activity at different poles of enterocytes of cattle during the fetal period of ontogenesis are shown and analyzed. The work was performed on Holstein cattle aged two to nine months. The activity of hydrolytic and transport enzymatic systems in the basolateral membrane of cattle enterocytes depends less on the morphometric parameters of the large intestine than in the apical membrane. It is proved that during the fruitful period of ontogenesis there are dynamic transformations of enzyme systems of enterocytes of the large intestine of cattle which are connected with morphological changes of the mucous membrane of the large intestine. The activity of hydrolytic and transport enzymes in different domains of the plasma enterocytes of fetal cattle is interrelated with the morphometric parameters of the colon, in particular. the activity of alkaline phosphatase, γ-glutamyltransferase, Na+, K+-ATPase, Ca2 +, Mg2 +-ATPase and Mg-ATPase in the apical membrane of enterocytes is inversely related to the thickness of the intestinal wall with villi and the mucous membrane of the cavity (P ≤ 0.01–0.001). A significant correlation was found between the activity of alkaline phosphatase, γ-glutamyltransferase, Na+, K+-ATPase, Ca2 +, Mg2 +-ATPase and Mg-ATPase in the apical membrane of enterocytes with villi height (P ≤ 0.05). The activity of hydrolytic and transport enzymatic systems in the basolateral membrane of bovine fetal enterocytes depends to a lesser extent on the morphometric parameters of the large intestine than in the apical membrane. In particular, only the activity of alkaline phosphatase and γ-glutamyltransferase is inversely related to the thickness of the intestinal wall with villi and the mucous membrane of the jejunum with villi (P ≤ 0.01–0.001). The activity of transport enzymes is directly related to the morphometric parameters of the colon (P ≤ 0.01). The activity of these enzymes in the basolateral membrane does not significantly depend on the width of the villi, but is dependent on their height.

The UN Secretary-General and the Security Council

2018 ◽  
Keyword(s):  
United Nations ◽  
Security Council ◽  
United Nations Security Council ◽  
Secretary General ◽  
Organizational Relationships ◽  
Dynamic Relationship ◽  
International Mediation ◽  
The United Nations ◽  
The Relationship

The United Nations Secretary-General and the United Nations Security Council spend significant amounts of time on their relationship with each other. They rely on each other for such important activities as peacekeeping, international mediation, and the formulation and application of normative standards in defense of international peace and security—in other words, the executive aspects of the UN’s work. The edited book The UN Secretary-General and the Security Council: A Dynamic Relationship aims to fill an important lacuna in the scholarship on the UN system. Although there exists an impressive body of literature on the development and significance of the Secretariat and the Security Council as separate organs, an important gap remains in our understanding of the interactions between them. Bringing together some of the most prominent authorities on the subject, this volume is the first book-length treatment of this topic. It studies the UN from an innovative angle, creating new insights on the (autonomous) policy-making of international organizations and adding to our understanding of the dynamics of intra-organizational relationships. Within the book, the contributors examine how each Secretary-General interacted with the Security Council, touching upon such issues as the role of personality, the formal and informal infrastructure of the relationship, the selection and appointment processes, as well as the Secretary-General’s threefold role as a crisis manager, administrative manager, and manager of ideas.

scholarly journals Comparison of the sensitivity of Western blotting between PVDF and NC membranes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yufang Xiang ◽  
Yuanyuan Zheng ◽  
Shaobo Liu ◽  
Gang Liu ◽  
Zhi Li ◽  
...  
Keyword(s):  
Western Blotting ◽  
Polyvinylidene Fluoride ◽  
Staining Intensity ◽  
Detection Sensitivity ◽  
Key Factors ◽  
Post Translational Modifications ◽  
Factors Affecting ◽  
Molecular Weights ◽  
Direct Blue ◽  
The Relationship

AbstractWestern blotting (WB) is one of the most widely used techniques to identify proteins as well as post translational modifications of proteins. The selection of electroblotted membrane is one of the key factors affecting the detection sensitivity of the protein which is transferred from gel to membrane in WB. The most common used membranes are polyvinylidene fluoride (PVDF) and nitrocellulose (NC) membranes. Which membrane of these two is more suitable for WB has not been reported so far. Here, by incubating proteins which were transferred to PVDF or NC membranes with a series of antibodies and different types of lectins, we investigated the relationship between the binding ability of these two membranes to proteins or glycoproteins and the molecular weight of the target protein. The antibody re-probed ability of the two membranes was also explored. Moreover, we verified the above results by directly incubating proteins having different molecular weights onto PVDF or NC membranes. Bound proteins were stained with direct blue-71, and the staining intensity was quantitated by scanning and densitometry.

scholarly journals Relating Competitive Golfers’ Perceived Emotions and Performance

2021 ◽  
pp. 003151252110059
Author(s):  
Erik Lundkvist ◽  
Henrik Gustafsson ◽  
Gunilla Björklund ◽  
Paul Davis ◽  
Andreas Ivarsson
Keyword(s):  
Perceived Control ◽  
Performance Outcomes ◽  
Skill Level ◽  
Complex Nature ◽  
Moderating Effects ◽  
Dynamic Relationship ◽  
And Performance ◽  
Player Skill ◽  
Task Oriented ◽  
The Relationship

The present study examined relationships between golfers’ self-perceived emotions (e.g., irritability, nervousness, tension), task-oriented coping, perceived control, and performance during a golf competition. We implemented a process-oriented golf analysis in which competitors rated these variables hole-by-hole in a competitive golf round. Within a two-level Bayesian multivariate autoregressive model, we showed that (a) within persons, emotions and task-oriented coping were reactions that stemmed from performance on the previous hole; and (b) between persons, player skill level predicted both better scores and the ability to limit the influence of negative affect on performance. These findings highlight the complex nature of the relationship between emotions and performance. Future studies might use a similarly ecologically valid research design to more precisely measure aspects of time and potentially moderating effects of player skill level and personality. An increased understanding of the dynamic relationship between emotions and performance can promote the development of effective psychological interventions for optimal performance outcomes.

scholarly journals Inhibition studies of alkaline phosphatase in hard tissue-forming cells.

1975 ◽  
Vol 23 (5) ◽  
pp. 342-347 ◽  
Author(s):  
A Linde ◽  
B C Magnusson
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Adenosine Triphosphatase ◽  
Ruthenium Red ◽  
Inorganic Pyrophosphatase ◽  
Alkaline Ph ◽  
Hard Tissue ◽  
Assay Condition ◽  
Phosphatase Inhibitors ◽  
Inhibition Studies

The effects of the alkaline phosphatase inhibitors levamisole and R 8231 on p-nitro-phenylphosphatase, inorganic pyrophosphatase and adenosine triphosphatase (ATPase) activities in dentingenically active odontoblasts were studied. The p-nitrophenylphosphatase and inorganic pyrophosphatase activities were inhibited, while 40% of the ATP-splitting enzyme activity remained under the assay condition used. This finding, togeather with earlier studies, indicates that at least two different phosphatase are active at alkaline pH in hard tissue-forming cells; on nonspecific alkaline phosphatase and one specific ATPase. The ATPase activity is uninfluenced by ouabain and ruthenium red and is activated by Ca-2+ ions.

Dibutyryl cAMP activates bumetanide-sensitive electrolyte transport in Malpighian tubules

1991 ◽  
Vol 261 (3) ◽  
pp. C521-C529 ◽  
Author(s):  
J. L. Hegarty ◽  
B. Zhang ◽  
T. L. Pannabecker ◽  
D. H. Petzel ◽  
M. D. Baustian ◽  
...  
Keyword(s):  
Yellow Fever ◽  
Apical Membrane ◽  
Basolateral Membrane ◽  
Fluid Secretion ◽  
Ringer Solution ◽  
Malpighian Tubules ◽  
Membrane Voltage ◽  
Dibutyryl Camp ◽  
K Secretion ◽  
Transepithelial Voltage

The effects of dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP) and bumetanide (both 10(-4) M) on transepithelial Na+, K+, Cl-, and fluid secretion and on tubule electrophysiology were studied in isolated Malpighian tubules of the yellow fever mosquito Aedes aegypti. Peritubular DBcAMP significantly increased Na+, Cl-, and fluid secretion but decreased K+ secretion. In DBcAMP-stimulated tubules, bumetanide caused Na+, Cl-, and fluid secretion to return to pre-cAMP control rates and K+ secretion to decrease further. Peritubular bumetanide significantly increased Na+ secretion and decreased K+ secretion so that Cl- and fluid secretion did not change. In bumetanide-treated tubules, the secretagogue effects of DBcAMP are blocked. In isolated Malpighian tubules perfused with symmetrical Ringer solution, DBcAMP significantly hyperpolarized the transepithelial voltage (VT) and depolarized the basolateral membrane voltage (Vbl) with no effect on apical membrane voltage (Va). Total transepithelial resistance (RT) and the fractional resistance of the basolateral membrane (fRbl) significantly decreased. Bumetanide also hyperpolarized VT and depolarized Vbl, however without significantly affecting RT and fRbl. Together these results suggest that, in addition to stimulating electroconductive transport, DBcAMP also activates a nonconductive bumetanide-sensitive transport system in Aedes Malpighian tubules.

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