scholarly journals Association of TGF-β2 Gene Polymorphism with Salmonella pullorum Bacterial Infection Resistance in Tolaki Chickens

2020 ◽  
pp. 46-54
Author(s):  
Muh Akramullah ◽  
Cece Sumantri ◽  
Niken Ulupi
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Growth Factor ◽  
Bacterial Infection ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Infection Resistance ◽  
Salmonella Pullorum ◽  
Growth Factor Beta ◽  
Beta 2 ◽  
Leucocyte Concentration

Transformation growth factor-beta 2 gene (TGF-β2) is a gene which belongs to the cytokine gene group. The present study aimed to identify Single Nucleotide Polymorphism (SNP) g. 640 T> C of Transformation growth factor-beta 2 gene (TGF-β2) and associate its genotypes with Salmonella pullorum Bacterial Infection Resistance in Tolaki Chickens. This study used blood samples collected from 70 chickens. Discussion was conducted on leucocyte concentration, leucocyte differentiation (heterophils, monocytes, lymphocytes, eosinophils, basophils and H/L ratio) and clearance test. Identification of Single Nucleotide Polymorphism of Transformation growth factor-beta 2 gene (TGF-β2) was performed by PCR-RFLP method. Association of (SNP) g. 640 T> C of Transformation growth factor-beta 2 gene (TGF-β2) with leukocyte profiles and their differentiation and resistance to bacterial infections was carried out using analysis of variance with GLM (General Linear Model). SNP g.640 T> C of Transformation growth factor-beta 2 gene (TGF-β2) was polymorphic and was in Hardy-Weinberg equilibrium. Based on molecular and biological testing, Tolaki chickens in genotypes (TT, TC and CC) have normal leucocyte concentration and differenciation and resistant to Salmonella pullorum.

A vascular endothelial growth factor A genetic variant is associated with improved ventricular function and transplant-free survival after surgery for non-syndromic CHD

2017 ◽  
Vol 28 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Constantine D. Mavroudis ◽  
Daniel Seung Kim ◽  
Nancy Burnham ◽  
Alexandra H. Morss ◽  
Jerry H. Kim ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Single Nucleotide Polymorphism ◽  
Growth Factor ◽  
Ventricular Function ◽  
Vascular Endothelial ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Free Survival ◽  
Endothelial Growth Factor

AbstractBackgroundWe have previously shown that the minor alleles of vascular endothelial growth factor A (VEGFA) single-nucleotide polymorphism rs833069 and superoxide dismutase 2 (SOD2) single-nucleotide polymorphism rs2758331 are both associated with improved transplant-free survival after surgery for CHD in infants, but the underlying mechanisms are unknown. We hypothesised that one or both of these minor alleles are associated with better systemic ventricular function, resulting in improved survival.MethodsThis study is a follow-up analysis of 422 non-syndromic CHD patients who underwent neonatal cardiac surgery with cardiopulmonary bypass. Echocardiographic reports were reviewed. Systemic ventricular function was subjectively categorised as normal, or as mildly, moderately, or severely depressed. The change in function was calculated as the change from the preoperative study to the last available study. Stepwise linear regression, adjusting for covariates, was performed for the outcome of change in ventricular function. Model comparison was performed using Akaike’s information criterion. Only variables that improved the model prediction of change in systemic ventricular function were retained in the final model.ResultsGenetic and echocardiographic data were available for 335/422 subjects (79%). Of them, 33 (9.9%) developed worse systemic ventricular function during a mean follow-up period of 13.5 years. After covariate adjustment, the presence of the VEGFA minor allele was associated with preserved ventricular function (p=0.011).ConclusionsThese data support the hypothesis that the mechanism by which the VEGFA single-nucleotide polymorphism rs833069 minor allele improves survival may be the preservation of ventricular function. Further studies are needed to validate this genotype–phenotype association and to determine whether this mechanism is related to increased vascular endothelial growth factor production.

scholarly journals Genetic variation and identification of single nucleotide polymorphism of insulin-like growth factor- 1 gene in Tilapia guineensis

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Esther Ukenye ◽  
IWALEWA MEGBOWON ◽  
OLALEKAN OGUNTADE ◽  
TOPE OKETOKI ◽  
OLUWAFEMI AMUSA ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Single Nucleotide Polymorphism ◽  
Genetic Variation ◽  
Growth Factor ◽  
Coastal Waters ◽  
Growth Traits ◽  
Selection Marker ◽  
Insulin Like Growth Factor ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide

Abstract. Ukenye E, Megbowon I, Oguntade O, Oketoki T, Amusa O, Usman A, Sokenu B, Adeleke R, Joseph B, Omatah C. 2020. Genetic variation and identification of single nucleotide polymorphism of insulin-like growth factor- 1 gene in Tilapia guineensis Biodiversitas 21: 5317-5321. Tilapia guineensis is an important cichlid species of West African coastal waters with good nutritional, economic, and aquaculture relevance. The knowledge of the genetic basis of variation in growth traits in Tilapia fish is of great importance to support genetic improvement in the context of aquaculture. In this study, regions of the Tilapia guineensis IGF-1 gene were sequenced, aligned and compared across populations to identify single nucleotide polymorphism and genetic diversity among four populations of T. guineensis in South-west Nigerian coastal waters. A total of thirty-four SNPs were identified across the studied populations and were detected in the forward reaction with twenty-two transversions and twelve transitions. Badagry population showed the highest genetic diversity with the highest molecular diversity indices; number of polymorphic sites, pairwise differences, number of segregating sites and nucleotide diversity while the least diverse population was Pepe. Analysis of molecular variance (AMOVA) revealed that genetic variation was mostly within populations. This finding provides more information regarding variation in insulin growth factor I of T. guineensis and will encourage association study for production traits that will inform useful selection marker for breeding program.

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