scholarly journals Prevalence of Occult Hepatitis B Virus Infections Among Kidney Transplant Patients in Khartoum State, Sudan

2020 ◽  
pp. 80-84
Author(s):  
Mashaer M Mustafa ◽  
Abdel Rahim M El Hussein ◽  
Isam M Elkhidir ◽  
Khalid A Enan
Keyword(s):  
Hepatitis B Virus ◽  
Renal Transplant ◽  
Hepatitis B ◽  
Hbv Dna ◽  
Competitive Elisa ◽  
Occult Hepatitis ◽  
Transplant Patients ◽  
B Virus ◽  
Occult Hepatitis B ◽  
Renal Transplant Patients

This study was carried out to detect occult hepatitis B virus (OHB) among renal transplant patients in Khartoum State, Sudan. Antigen capture enzyme linked immunosorbent assay (ELISA) competitive ELISA and polymerase chain reaction (PCR) were used to respectively detect hepatitis B surface antigen (HBsAg), ; Hepatitis B core antibody (HBcAb) and hepatitis B virus (HBV) DNA in 100 plasma samples collected from patients during the period from May to October 2018 . Out of the 100 patients sampled, 70 were males and 30 were females (age 15 to 65 years) and none of these patients showed signs of clinical hepatitis. The results showed that 3 out of the 100 samples were positive for HBsAg, and were subsequently excluded from the study. Out of the remaining HBsAg negative 97 samples, 39(40.2%) showed positive HBcAb and none (0%) tested positive to HBV DNA using competitive ELISA and PCR, respectively. These results indicated that more investigations including more patients from other transplant centers are needed to fully elucidate the situation of occult hepatitis B in renal transplant patients in Sudan.

scholarly journals Occult Hepatitis B Virus Infection among HIV Positive Patients in Nigeria

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Oluyinka Oladele Opaleye ◽  
Adeolu Sunday Oluremi ◽  
Adetona Babatunde Atiba ◽  
Moses Olubusuyi Adewumi ◽  
Olatunji Victor Mabayoje ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Virus Infection ◽  
Hepatitis B ◽  
Hbv Dna ◽  
Hiv Positive ◽  
Hepatitis B Virus Infection ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B ◽  
Detectable Hbsag

HIV has been known to interfere with the natural history of hepatitis B virus (HBV) infection. In this study we investigate the prevalence of occult hepatitis B virus infection (OBI) among HIV-infected individuals in Nigeria. Overall, 1200 archived HIV positive samples were screened for detectable HBsAg using rapid technique, in Ikole Ekiti Specialist Hospital. The HBsAg negative samples were tested for HBsAg, anti-HBc, and anti-HCV by ELISA. Polymerase chain reaction was used for HBV DNA amplification and CD4 counts were analyzed by cytometry. Nine hundred and eighty of the HIV samples were HBsAg negative. HBV DNA was detected in 21/188 (11.2%) of patients without detectable HBsAg. CD4 count for the patients ranged from 2 to 2,140 cells/μL of blood (mean = 490 cells/μL of blood). HCV coinfection was detected only in 3/188 (1.6%) of the HIV-infected patients (P>0.05). Twenty-eight (29.2%) of the 96 HIV samples screened were positive for anti-HBc. Averagely the HBV viral load was <50 copies/mL in the OBI samples examined by quantitative PCR. The prevalence of OBI was significantly high among HIV-infected patients. These findings highlight the significance of nucleic acid testing in HBV diagnosis in HIV patients.

scholarly journals Occult hepatitis B virus infection among hemodialysis patients

2018 ◽  
Vol 91 (2) ◽  
Author(s):  
Rahil Nahid Samiei ◽  
Somayeh Shokri ◽  
Shahab Mahmoudvand ◽  
Manoochehr Makvandi ◽  
Heshmatollah Shahbazian ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hbv Dna ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hepatitis B Infection ◽  
Nested Polymerase Chain Reaction ◽  
Occult Hepatitis ◽  
B Virus ◽  
The World ◽  
Occult Hepatitis B

Hepatitis B virus is a major public health impasse all over the world. Recently a new form of hepatitis B infection named Occult hepatitis B Infection (OBI) has appeared globally. The OBI is defined as the presence of HBV DNA in the liver and/or blood in the absence of detectable serum HBsAg with/without anti-HBc or anti-HBs. The prevalence of OBI has been reported in hemodialysis (HD) patients in different regions of the world. Thus, this study investigated the prevalence of OBI among HD patients. The cross-sectional study was carried out on 84 HD patients. These sera were checked for HBsAg, HBc-IgG assessment using Enzyme linked immunosorbent assay. The DNA was extracted from the sera samples and tested for HBVDNA detection using Nested Polymerase Chain Reaction (Nested PCR). The liver function tests including serum alanine aminotransferase and aspartate aminotransferase levels were carried out for all the HD individuals. 52/84(61.9%) of HD were males and 32/84 (38.1%) were females. The patient’s age ranged from 25 to 64 with a mean age of 52.4±15.2 years. HBsAg and HBc-IgG were detected in 1(1.1%) female. 2 (2.4%; a female and a male) patients were positive for HBsAg. 14/84 (16.7%; 6 female and 8 male) HD patients were positive for anti-HBc but negative for HBsAg, among them 4(28.6%; 2 female and 2 male) cases were positive for HBV DNA, indicating the presence of OBI in HD patients. Even distribution of OBI among the HD was found in 2(2.36%) male and 2(2.36%) female (P>.0.05). In the present study the moderate rate of 4.76% OBI has been observed in HD patients. The prevalence of seropositive OBI among the gender was 2(2.36%) male and 2(2.36%) female. The seronegative OBI have not been detected in the present study but requires further investigation. In this study the affliction of OBI in HD patients is not clear.

Detection of occult hepatitis B virus in patients undergoing chemotherapy in Iran

2021 ◽  
Author(s):  
Paniz Shirmast ◽  
Mahdi Abedinzade Shahri ◽  
Salar Pashangzadeh ◽  
Hessam Mirshahabi ◽  
Elham Samadi ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Mortality Rate ◽  
Hepatitis B ◽  
Cancer Patients ◽  
Hbv Dna ◽  
Hepatitis B Infection ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B ◽  
Life Threatening

Aim: Occult hepatitis B infection (OBI) is life threatening and has a high mortality rate despite applying antiviral treatments in cancer patients. This study aimed to investigate the prevalence of OBI in patients undergoing chemotherapy in Iran. Materials & methods: A total of 342 patients undergoing chemotherapy were enrolled. OBI detection in anti-HBc positive individuals was conducted using nested-PCR. Results: Among 342 subjects, 103 (30.1%) were positive for anti-HBc. Fifteen (14.6%) cases of 103 anti-HBc positive samples were also positive for HBsAg. Overall, HBV DNA was positive in three (3.4%) of 88 anti-HBc subjects. Conclusion: Our results indicated that OBI might occur in almost one in 25 anti-HBc-positive patients undergoing chemotherapy.

Prevalence of Occult Hepatitis B Infection among HBsAg Negative Blood Donors in Golestan Province

2019 ◽  
Author(s):  
Rana Tabar Asad Laleh ◽  
Zohreh Sharifi ◽  
Akbar Pourfathollah ◽  
Shahram Samei
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Donors ◽  
Hbv Dna ◽  
High Rate ◽  
Analytical Sensitivity ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B ◽  
Golestan Province

Background and Aims: Occult hepatitis B virus infection (OBI) is known as an important source of hepatitis B virus (HBV) infection. It is categorized as Hepatitis B surface antigen (HBsAg) not being present and low DNA viral load in serum. In this study, an attempt was made to investigate the outbreak of anti-HBc and OBI among the HBsAg-negative donors in Golestan province. Materials and Methods: The present cross-sectional experiment was conducted on 3500 voluntary blood donors in Golestan province to examine the presence of human immunodeficiency viruses Ag-Ab, HBsAg, and hepatitis C virus Ab. Then, samples with negative results for the mentioned tests were screened for total HBc antibody (IgM-IgG) through ELISA technique. Afterward, HBV-DNA extraction and R-T PCR assay were conducted for all HBsAg negative samples by using Real ART HBV LC PCR kit on a Light Cycler instrument. Results: The study participants included 3255 (93%) male and 245 (7%) female. In general, 385 (11%) out of 3500 samples were anti-HBc positive. HBV-DNA results for every sample with either positive or negative anti-HBc were found to be negative. Conclusions: As the area under study has a high rate of anti-HBc outbreak (11%) without the presence of HBV-DNA, anti-HBc screening can cause blood donor deferrals and limit blood supply; therefore, the HBsAg test with high analytical sensitivity is recommended for HBV screening in this area. Regarding the cost analyses and also the status of HBV endemicity, HBsAg test along with ID-NAT is preferable, if possible, for improving blood safety.

PREVALENCE OF OCCULT HEPATITIS B INFECTION IN RENAL TRANSPLANT PATIENTS.

2004 ◽  
Vol 78 ◽  
pp. 679
Author(s):  
A A. Peres ◽  
E A. Dias ◽  
M Chesky ◽  
L F. Jobim ◽  
L F. Gonçalves ◽  
...  
Keyword(s):  
Renal Transplant ◽  
Hepatitis B ◽  
Hepatitis B Infection ◽  
Occult Hepatitis ◽  
Transplant Patients ◽  
Occult Hepatitis B ◽  
Renal Transplant Patients

scholarly journals Characterization of Occult Hepatitis B Virus Infection Among Iranian Patients with Behcet's Disease; Correlation with Clinical Status

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Farhad Shahram ◽  
Saied Ghorbani ◽  
Mahdi Mahmoudi ◽  
Maassoumeh Akhlaghi ◽  
Zohreh Jadali ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Clinical Data ◽  
Disease Duration ◽  
Clinical Manifestations ◽  
Hbv Dna ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B ◽  
The Mean

Background: Behcet's disease (BD) is a chronic multisystem vasculitis with an unknown etiology. During the past years, several reports are published on the occult hepatitis B infection (OBI), the presence of hepatitis B virus (HBV) DNA in the absence of HBsAg, in rheumatic diseases. Objectives: The current study aimed to, firstly, investigate the prevalence of OBI in patients with BD, and, secondly, its potential association with the clinical and therapeutic status of BD. Methods: HBV serological markers and HBV DNA were evaluated in 220 consecutive BD patients to detect OBI. Demographic and clinical data of OBI positive and negative groups were compared. Results: The mean age of patients was 39.24 (± 10.57), and 134 (62.9%) were male. The mean disease duration was 14.13 (± 8.63) years. No HBsAg positive case was found, but HBV DNA was found in 19 (8.6%) patients. The median viral load value was 475.84 copy/mL. We compared clinical data of 10 OBI positive and 156 OBI negative BD patients with complete and accessible data. There was no difference between the two groups concerning demographic characteristics (age, sex, and disease duration), different clinical manifestations, or types of medications (immunomodulatory, cytotoxic, and corticosteroids). Conclusions: This is the first study showing a rather high prevalence of OBI among BD patients. We did not find any correlation between OBI positivity and different clinical manifestations, medications, or HLA-B51. Further studies are needed on a larger group of patients and by molecular HBV evaluation (as well as serologic) regarding this possible association.

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