scholarly journals Polymorphism Analysis of TGF-β2 Gene and Its Association with Body Weight and Body Size Measurements of Tolaki Chicken

Author(s):  
Muhammad Amrullah Pagala ◽  
Rusli Badaruddin ◽  
Deki Zulkarnain

This study aims to identify the transforming growth factor-beta2 (TGF-β2) gene then associate it with body weight and body size measurements in Tolaki chicken. Tolaki chicken used in this study amounted to 60 hens, the results of hatching in the Lab. Halu Oleo University Poultry Unit, Kendari. Maintenance from 6 weeks to 12 weeks. Observed data include; body weight, back length, chest circumference, shank length, tibia length, femur length, and wing length, were measured every week during the study. The diversity of the TGF-β2 gene was identified using the PCR-RFLP method. The genotype frequency values ??of TT (39), TC (18), and CC (3), respectively 0.65, 0.30, and 0.05. The value of frequency of T allele and C allele are 0.80 and 0.20 of the total population, respectively. The results of the chi-square TGF-β2 gene are in Hardy-Weinberg balance. Statistical results showed no significant difference (P> 0.05) between the TT, TC and CC genotypes in the TGF-β2 gene with the weight parameters and body measurements of the Tolaki chicken.

2019 ◽  
Vol 19 (2) ◽  
pp. 122-128
Author(s):  
Kusnadidi Subekti ◽  
Dedi Duryadi Solihin ◽  
Rudi Afnan ◽  
Asep Gunawan ◽  
Cece Sumanri

ABSTRAK. Tujuan dari penelitian ini adalah untuk mengidentifikasi pengaruh polimorfisme gen heat shock protein 70 (HSP70) terhadap sifat toleransi panas. Genotiping dilakukan pada 110 itik lokal dari 4 populasi itik Sumatera Barat (pitalah, bayang, kamang, dan payakumbuh). Enzim restriksi yang digunakan adalah SacII. Cekaman panas dilakukan selama 1 jam dengan sampel itik lokal sebanyak 24 ekor untuk menganalisa status hematologi. PCR-RFLP dan Sanger sequencing assay digunakan untuk mengidentifikasi polimorfisme. Analisis polimorfisme dilakukan dengan menggunakan Software MEGA 7 dan pengaruhnya dianalisis dengan ANOVA menggunakan software SAS 9.4. Produk amplifikasi yang dihasilkan yaitu 466 pasang basa. Lokus HSP70/SacII mengenali satu situs SNP (Single nucleotide Polymorphism) g.1702TC, menghasilkan dua alel (T dan C) dan tiga genotype (TT, TC, CC). Gen HSP70/SacII bersifat polimorfik pada semua populasi. Hasil penelitian menunjukkan bahwa alel T memiliki frekuensi yang lebih tinggi daripada alel C pada semua populasi. Analisis chi-kuadrat (χ2) menunjukkan bahwa semua populasi itik lokal memenuhi kaidah kesetimbangan Hardy-Weinberg. Pengaruh genotipe gen HSP70 terhadap status hematologi menunjukkan perbedaan (P0,05) terhadap nilai heterofil dan nilai H/L dengan genotipe CT menunjukkan toleransi panas yang lebih baik dibanding genotipe lainnya. Gen HSP70/SacII dapat dijadikan marka molekuler sifat toleransi panas pada itik lokal berdasarkan keragaman dan pengaruhnya terhadap status hematologi.  (The effect of heat shock protein 70 (HSP70) SacII gene polymorphism on heat tolerance of West Sumatera local duck) ABSTRACT. The objective of this study was to identify the effect of HSP70/SacII gene polymorphism associated with the thermotolerance trait. Genotyping was performed on 110 local duck from the West Sumatera ducks population (pitalah, bayang, kamang, and payakumbuh). The restriction enzyme used was SacII. Heat stress was done for 1 hour with samples used as much as 24 birds to analyze hematological status. PCR-RFLP and Sanger sequencing assays were used to identify polymorphism. Analysis of polymorphism was conduct by MEGA 7 software and its effect is analyzed with ANOVA using Statistical SAS 9.4 software. The product of amplification was 466 bp. HSP70/SacII recognized one SNP g.1702TC, producing two alleles (T and C) and three genotypes (TT,TC,CC). The HSP70/SacII locus were polymorphic in all population. T allele had a higher frequency than C allele in all populations. The analysis of chi-square (χ2) showed that all local ducks population were fit with Hardy-Weinberg equilibrium. The effect of HSP70/SacII gene on hematological status showed differences (P0,05) of heterophil and H/L ratio with CT genotype show better heat tolerance than other genotypes. HSP70/SacII gene can be used as a marker of heat tolerance in local ducks based on polymorphism and its effect on hematological status.


1959 ◽  
Vol 36 (1) ◽  
pp. 113-119
Author(s):  
A. B. GILBERT

1. The influence of body size and sex on the total osmotic pressure (O.P.) and blood conductivity of the shore crab was investigated. 2. In both sexes the O.P. fell steadily as body weight increased. 3. At any body weight the O.P. of the blood of male crabs was significantly higher that that of females. 4. Blood conductivity increased in both sexes until a maximum was reached at a weight of about 35 g. Thereafter the conductivity fell as the weight increased. 5. There was no significant difference in blood conductivity between male and female crabs below 35 g. body weight. Above 35 g. the conductivity of males was significantly higher than that of females.


2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Danika Bannasch ◽  
Thomas Famula ◽  
Jonas Donner ◽  
Heidi Anderson ◽  
Leena Honkanen ◽  
...  

Abstract Background Dog breeds are known for their distinctive body shape, size, coat color, head type and behaviors, features that are relatively similar across members of a breed. Unfortunately, dog breeds are also characterized by distinct predispositions to disease. We explored the relationships between inbreeding, morphology and health using genotype based inbreeding estimates, body weight and insurance data for morbidity. Results The average inbreeding based on genotype across 227 breeds was Fadj = 0.249 (95% CI 0.235–0.263). There were significant differences in morbidity between breeds with low and high inbreeding (H = 16.49, P = 0.0004). There was also a significant difference in morbidity between brachycephalic breeds and non-brachycephalic breeds (P = 0.0048) and between functionally distinct groups of breeds (H = 14.95 P < 0.0001). Morbidity was modeled using robust regression analysis and both body weight (P < 0.0001) and inbreeding (P = 0.013) were significant (r2 = 0.77). Smaller less inbred breeds were healthier than larger more inbred breeds. Conclusions In this study, body size and inbreeding along with deleterious morphologies contributed to increases in necessary health care in dogs.


2020 ◽  
Vol 46 (5) ◽  
Author(s):  
A. E. Jubril ◽  
T. R. Fayeye ◽  
A. A. Ademola ◽  
H. H. Gunn

This study was conducted to determine auto-sexing potential in Rhode Island, Nigerian local chicken and their reciprocal crosses. A total of 241 eggs were set in the incubator to determine the fertility, hatchability, % Hatch, % dead in shell, % dead in cell and % deformed chicks in the four genotypes (Rhode Island Red (RIR) x Rhode Island White (RIW), Rhode Island Red (RIR) x Rhode Island White (RIW), Nigerian Local Red (NLR) x Rhode Island White (RIW) and Nigerian Local Red (NLR) x Nigerian Local White (NLW). Only 94 eggs were hatched. RIRXRIW crossbred chicks had the highest percentage fertility of 88.89% followed by RIRxRIW (86.27%), NLRXRIW (77.36%) and NLRxNLW (72.31%), respectively. RIRxNLW had the highest percentage hatchability of 65.19%, followed by RIRxRIW (51.56%), NLRxNLW (51.06%) and NLRxRIW (24.39%). It was observed in the hatch-out analysis that the cross between NLRxRIW had the highest percentage of dead in shell (29.27%) followed by RIRxRIW (17.19%), NLRxNLW (17.02%) and RIRxNLW (11.36%), respectively. The highest percentage of dead in cell was recorded in NLRxRIW crossbred (43.33%), followed by NLRxNLW (31.92%), RIRxRIW (28.13%) and RIRxNLW (15.91%). The observed deformed chicks were highest in NLRxNLW (20.85%) followed by RIRxNLW (10.34%), RIRxRIW (6.06%) and NLRxNLW (0%), respectively. Also observed was the occurrence of bangers with NLRxRIW, having the highest occurrence of bangers (9.08%) and with NLRxNLW having no occurrence of bangers. The records of weekly body weight were taken on the 94 chicks and chi square analysis was used to test colour inheritance of chicks. Significant (P<0.05) difference was observed among genotypes in body weight of chicks at hatch and from weeks 1 to 8 weeks of age. The observed changes on body weight from 0 to 8th week of age of chicks showed significant difference across the four different genetic crossed groups (P<0.05) and weight at the 8th week showed that the cross between RIRxRIW (216.93g) had better in growth as compared to the cross between NLRxRIW (202.75g) and NLRxNLW (193.17g) which were statistically similar (P>0.05) and RIRxNLW (179.75g) crossbred chicks which had the lowest bodyweight at 8 weeks of age. The chi square (X2) analysis revealed that both RIRxRIW and NLRxRIW crossbred chicks were autosexed. The study concluded that selection for plumage colour showed great potential in determining the probability of chicks being autosexed. Also, pure bred of RIRXRIW and reciprocal cross of NLRXRIW showed great potential of producing autosexed chicks, while results on the direct crosses of NLRXNLW and RIRXNLW suggest that the pattern of inheritance of plumage colour is not simple. The study recommends further investigation to further ascertain the mode of plumage colour inheritance in the Nigerian Local Chickens.


2020 ◽  
Vol 2 (2) ◽  
pp. 54-61
Author(s):  
Fahrijal Moyiu

The purpose of this study was to determine the quantitative nature of male and female Weris (Gallirallus philippensis) in Wonosari District, Boalemo Regency. The study was conducted in August-October 2019. This study used a sample of 20 Weris birds consisting of 10 males and 10 females. The method used is a survey method, through direct observation in the field. Observations on the quantitative nature of Weris birds (Gallirallus philippensis) were carried out directly at the research location. The data obtained were analyzed descriptively. The results showed the average body size of male Weris birds were: body weight 171.9 gr, femur length 5.42 cm, tibia length 6.81 cm, long shank 4.56 cm, third finger length 3.48 cm, maxilla length 3, 14 cm, wing length 11.20 cm, neck bone length 7.07 cm, shank circumference 1.68 cm. Body weight of male weris 171.9 ± 11.06, females 135.90 ± 11.82 with diversity coefficient 6.43 and 8.70


Author(s):  
Anurag Gupta ◽  
Nihar Ranjan Mohanty ◽  
Avinash Tiwari ◽  
Shyamal Koley

Football is a sport composed of many athletic components and requires different physical attributes and skills from the individual. There are different playing positions which are goal keepers, defenders, mid fielders and strikers. Purpose of the study was to evaluate the relation between different playing positions with selected anthropometric variables and back muscle strength in state level football players. Design of the study was based on cross sectional research. Sample of 106 male footballers of age group 16-25 years were included in the study and were selected from various football clubs of Maharashtra, India. In results, statistically significant differences (p ≤0.015-0.001) were noted in height, weight, tibia length, femur length and total leg length between goalkeepers and defenders. Statistically significant differences (p ≤0.018-0.001) were noted in height, weight, tibia length, femur length, total leg length and trunk length between goalkeepers and mid fielders. Statistically significant differences (p ≤0.019-0.002) were noted in height, weight, tibia length and total leg length between goalkeepers and strikers. There was no statistically significant difference between defenders and mid fielders; and between the mid fielders and strikers. Keywords: anthropometry, back strength, football, kinetics.


2017 ◽  
Vol 84 (4) ◽  
pp. 221-225 ◽  
Author(s):  
Pier A. Della Camera ◽  
Simone Morselli ◽  
Gianmartin Cito ◽  
Giovanni Tasso ◽  
Andrea Cocci ◽  
...  

Introduction Mediterranean diet has shown a protective role against cardiovascular disease, diabetes, cancer onset, microvascular damage and dementia in many trials. Our purpose is the assessment of a correlation between physical activity, Mediterranean diet, body mass index (BMI), depression and erection disorder (ED). Methods After having signed disclaimer to the study participation, we administered the IIEF 15 questionnaire (International Index of Erectil Function), the Hamilton questionnaire for major depression, the Med-Diet Questionnaire, the Ipaq Questionnaire (International Index of Physical Activity) to 245 patients and calculated the BMI. Only 141 were eligible. We excluded patients with a history of smoking, with obesity from the second grade to rise, anorexia, hyperlipidemia, Induratio Penis Plastica, diabetes, cardiovascular and neurological disease, hypogonadism, prostatitis, diabetes, hypertension, psychiatric diseases and the history of radical prostatectomy and finally age >72 and <50 years or who were taking cholesterol-lowering medication. Patients were divided into two groups: 65 patients without ED and 76 patients with ED. Results We found a statistically difference in BMI between the groups. Adherence to Med-Diet showed a significant difference between the two groups at Student t-test and the Chi-square test. The Ipaq test and Hamilton test did not show statistical differences between the two groups neither for Student t-test nor for Chi-square test, but high levels seem to be protective factors. Conclusions Body weight and a healthy diet are protective factors against the ED, more than a sufficient physical activity. Depression has shown only a worsening tendency of the erection.


Author(s):  
Firda Arlina ◽  
Sabrina Husmaini ◽  
R. Rhoudha ◽  
W. R. Sardi ◽  
T. Rafian

Abstract This research was aimed to identifiaty qualitative and quantitative phenotypic polymorphism of Sikumbang Jonti Duck in Kecamatan Payakumbuh Timur Kota Payakumbuh Sumatera Barat. This research used 206 Sikumbang Jonti duck that were sexual maturity (22-48 weeks), divided of 50 males and 156 females. The qualitative traits observeted were head color, neck color, breaks color, back color, primary wings color, tail color, thigh color, bill color, and shank color. The quantitative traits observed were body weight (kg), beak width (cm), beak length (cm), neck length (cm), wing length (cm), femur length (cm), tibia length (cm), shank length (cm), back length (cm), number of primary wing feathers (strands), number of secondary wing feathers (strands), pelvic width (cm), and chest circumference (cm). The result showed that color of Sikumbang Jonti duck was dominated by white. Male Sikumbang Jonti duck had color head was white-black, and female had color head was white. In addition, the Sikumbang Jonti duck had green primary wing feathers like a beetle. The coefficient of diversity of the Sikumbang Jonti duck was low for beak width, tibia length (female), number of primary wing feathers, and number of secondary wing feathers, moderate value for body weight, beak length, neck length, wing length, femur length (female), length tibia (male), shank length, back length, perlvis width (females), and chest circumference (males), and high value for femur length (males). Keywords: Duck morphometric; Germplasm; Pattern color; Payakumbuh; Sumatera barat   Abstrak Penelitian ini bertujuan untuk mengindentifikasi keragaman fenotipe kualitatif dan  kuantitatif itik Sikumbang Jonti di Kecamatan Payakumbuh Timur Kota Payakumbuh Provinsi Sumatera Barat. Penelitian ini menggunakan 206 ekor itik Sikumbang Jonti yang sudah dewasa kelamin (22 – 48 minggu), terdiri dari 50 ekor jantan dan 156 ekor betina. Sifat kualitatif yang diamati adalah warna bulu head, warna bulu neck, warna bulu breaks, warna back, warna primary wings, warna tail, warna thigh, warna bill, dan warna shank. Sifat kuantitatif yang diamati adalah bobot badan (kg), lebar paruh (cm), panjang paruh (cm), panjang leher (cm), panjang sayap (cm), panjang femur (cm), panjang tibia (cm), panjang shank (cm), panjang punggung (cm), jumlah bulu sayap primer (helai), jumlah bulu sayap sekunder(helai), lebar pelvis (cm), dan lingkar dada (cm). Hasil menunjukkan warna bulu itik Sikumbang Jonti didominasi dengan warna bulu putih. Warna bulu kepala itik Sikumbang Jonti jantan berwarna putih-hitam, sedangkan itik Sikumbang Jonti betina berwarna putih. Selain itu, itik Sikumbang Jonti memiliki warna bulu sayap primer berwarna hijau seperti kumbang. Koefisien keragaman itik Sikumbang Jonti bernilai rendah untuk lebar paruh, panjang tibia (betina), jumlah bulu sayap primer, dan jumlah bulu sayap sekunder, bernilai sedang untuk bobot badan, panjang paruh, panjang leher, panjang sayap, panajng femur (betina), panjang tibia (jantan), panjang shank, panjang punggung, lebar perlvis (betina), dan lingkar dada (jantan), dan bernilai tinggi untuk panjang femur (jantan). Keragaman fenotipe kualitatif dan kuantitatif pada itik Sikumbang Jonti relatif seragam, kecuali pada fenotipe kuantitatif panjang femur pada itik Sikumbang Jonti jantan memiliki keragaman tinggi. Kata kunci: Morfometrik itik; Payakumbuh; Plasma nutfah; Sumatera barat; Warna bulu


2020 ◽  
Vol 1 (3) ◽  
pp. 6
Author(s):  
Muhammad Tahir ◽  
Aamer Ali Khattak ◽  
Erum Monis ◽  
Sana Gul

Objective: To perform genotyping for MTR A2756G polymorphism and identification of risk factors associated with head and neck squamous cell carcinoma (HNSCC). Study Design: Cross section, comparative study. Place and Duration of Study: The study was carried out at the Department of Biochemistry of Quaid-i- Azam University, Islamabad from October 2014 to August 2015. Materials and Methods: In this study, 292 diagnosed patients HNSCC and 324 normal individuals without any history of cancer were enrolled. Blood samples of patients and controls were collected in ethylenediamine tetra acetic acid (EDTA) and DNA was extracted using conventional method. All samples were genotyped for the MTR A2756G polymorphism using PCR-RFLP. Frequency of polymorphism was compared between HNSCC patients andcontrols. MultipleLogisticRegression(MLR)andchi-squaretestwasperformedtoexaminetheassociation of MTR A2756G polymorphism with risk factor. Results: Chi-square test of independence showed statistically significant difference among the variables of age, smoking and MTR A2756G genotype (p-value<0.05). Multivariate analysis showed that smoking (adjusted OR, 3.7; 95% CI, 2.3 – 6.0), age groups 41 – 50 years (adjusted OR, 3.6; 95% CI, .9 – 6.7) and > 60 years (adjusted OR, 3.5; 95% CI, 1.7 – 7.3), MTR 2756 AG genotype (adjusted OR, 2.1; 95% CI, 1.3 – 3.5) is associated with increased risk of HNSCC. Conclusion: The results suggest that the genetic polymorphism MTR A2756G is associated with the occurrence of HNSCC in the Pakistani population while the individuals between 40 to 50 years of age and those who are smokers are at a greater risk of developing HNSCC.


2020 ◽  
Vol 47 (2) ◽  
pp. 1-7
Author(s):  
O. H. Osaiyuwu ◽  
U. F. O. Bolaji ◽  
O. A. Adeyinka ◽  
M. O. Akinyemi ◽  
A. E. Salako

In livestock production, traditional methods of selection have always been the way to go. However, with the advent of genomics techniques, methods such as PCR-RFLP is been employed to identify single nucleotide polymorphism of likely candidate genes useful for livestock selection and improvement. Therefore, the aim of this study was to investigate the association of Melanocortin 4 Receptor (MC4R) gene polymorphism with the body weight ofrabbits. Seventy-four rabbits were used for this study consisting of six breeds; 26 Chinchilla, 3 Californian, 11 Dutch, 4 English Spot, 10 New Zealand White and 20 Fauve de Bourgogne (FDB) breeds. Blood samples were collected from the animals with needle and syringe and transferred unto FTA cards and stored away from light. Body weight measurements on the animals were recorded from 2 weeks to 20 weeks. PCR-RFLP analysis produced threegenotypes AA, AG, GG with genotype frequency of 0.14, 0.69 and 0.18 respectively. Allele frequency 0.48 and 0.52 for allele A and G respectively was obtained. Chi-square test showed that the population was not in Hardy-Weinberg equilibrium. Association analysis between the MC4R “c.101G>A” SNP and body weight of rabbit was tested using GLM procedure of SAS programme. Genotype GG had higher body weight (p<0.05) at 12, 16 and 20 weeks inthe Dutch rabbits while genotype AA recorded a higher (p<0.05) body weight value at 12 weeks in New Zealand White rabbits. More so, female Dutch rabbits had higher (p<0.05) mean weight than the males at 12, 16 and 20 weeks of age. Male New Zealand White breeds recorded higher (p<0.05) mean weight than the female animals. The result of the study showed that MC4R “c.101G>A” SNP was not associated with the body weight in the studied rabbit population, although, genotype AA had higher mean weight values at 12, 16 and 20 weeks of age than genotypes AG and GG. 


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