scholarly journals Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbata

2020 ◽  
Vol 64 ◽  
pp. 224-233
Author(s):  
A. CHAVEZ-CORTAZAR ◽  
M. MATA-ROSAS ◽  
K. OYAMA ◽  
M.S. SAMAIN ◽  
M. QUESADA
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Stability ◽  
Regenerated Plants ◽  
Magnolia Dealbata

scholarly journals Protocol development for somatic embryogenesis, SSR markers and genetic modification of Stipagrostis pennata (Trin.) De Winter

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Masoumeh Asadi-Aghbolaghi ◽  
Beata Dedicova ◽  
Sonali Sachi Ranade ◽  
Kim-Cuong Le ◽  
Farzad Sharifzadeh ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somaclonal Variation ◽  
Ssr Markers ◽  
Genetic Stability ◽  
Somatic Embryos ◽  
Large Scale ◽  
Scale Production ◽  
Ms Medium ◽  
Important Species ◽  
Regenerated Plants

Abstract Background Stipagrostis pennata (Trin.) De Winter is an important species for fixing sand in shifting and semi-fixed sandy lands, for grazing, and potentially as a source of lignocellulose fibres for pulp and paper industry. The seeds have low viability, which limits uses for revegetation. Somatic embryogenesis offers an alternative method for obtaining large numbers of plants from limited seed sources. Results A protocol for plant regeneration from somatic embryos of S. pennata was developed. Somatic embryogenesis was induced on Murashige & Skoog (MS) medium supplemented with 3 mg·L–1 2,4-D subsequently shoots were induced on MS medium and supplemented with 5 mg·L–1 zeatin riboside. The highest shoots induction was obtained when embryogenic callus derived from mature embryos (96%) in combination with MS filter-sterilized medium was used from Khuzestan location. The genetic stability of regenerated plants was analysed using ten simple sequence repeats (SSR) markers from S. pennata which showed no somaclonal variation in regenerated plants from somatic embryos of S. pennata. The regenerated plants of S. pennata showed genetic stability without any somaclonal variation for the four pairs of primers that gave the expected amplicon sizes. This data seems very reliable as three of the PCR products belonged to the coding region of the genome. Furthermore, stable expression of GUS was obtained after Agrobacterium-mediated transformation using a super binary vector carried by a bacterial strain LBA4404. Conclusion To our knowledge, the current work is the first attempt to develop an in vitro protocol for somatic embryogenesis including the SSR marker analyses of regenerated plants, and Agrobacterium-mediated transformation of S. pennata that can be used for its large-scale production for commercial purposes.

Attempts to eradicate graft-transmissible infections through somatic embryogenesis in Citrus ssp. and analysis of genetic stability of regenerated plants

2016 ◽  
Vol 148 (1) ◽  
pp. 85-95 ◽  
Author(s):  
Malika Meziane ◽  
Dajana Frasheri ◽  
Angela Carra ◽  
Messaoud Boudjeniba ◽  
Anna Maria D’Onghia ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Stability ◽  
Regenerated Plants

Genetic stability of regenerated plants via indirect somatic embryogenesis and indirect shoot regeneration of Carum copticum L.

2017 ◽  
Vol 97 ◽  
pp. 330-337 ◽  
Author(s):  
Mohsen Niazian ◽  
Seyed Ahmad Sadat Noori ◽  
Petr Galuszka ◽  
Masoud Tohidfar ◽  
Seyed Mohammad Mahdi Mortazavian
Keyword(s):  
Somatic Embryogenesis ◽  
Shoot Regeneration ◽  
Genetic Stability ◽  
Regenerated Plants ◽  
Indirect Somatic Embryogenesis ◽  
Carum Copticum

Genetic stability and phytochemical profiling of the in vitro regenerated plants of Angelica glauca Edgew.: an endangered medicinal plant of Himalaya

2018 ◽  
Vol 135 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Janhvi Mishra Rawat ◽  
Aakriti Bhandari ◽  
Susmita Mishra ◽  
Balwant Rawat ◽  
Ashok Kumar Dhakad ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Genetic Stability ◽  
Regenerated Plants ◽  
Endangered Medicinal Plant ◽  
Angelica Glauca

scholarly journals Erratum to: Plant Fidelity in Somatic Embryogenesis-Regenerated Plants

2016 ◽  
pp. E1-E1
Author(s):  
Hervé Etienne ◽  
Romain Guyot ◽  
Thierry Beulé ◽  
Jean-Christophe Breitler ◽  
Estelle Jaligot
Keyword(s):  
Somatic Embryogenesis ◽  
Regenerated Plants
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