Genetic stability of regenerated plants via indirect somatic embryogenesis and indirect shoot regeneration of Carum copticum L.

2017 ◽  
Vol 97 ◽  
pp. 330-337 ◽  
Author(s):  
Mohsen Niazian ◽  
Seyed Ahmad Sadat Noori ◽  
Petr Galuszka ◽  
Masoud Tohidfar ◽  
Seyed Mohammad Mahdi Mortazavian
Keyword(s):  
Somatic Embryogenesis ◽  
Shoot Regeneration ◽  
Genetic Stability ◽  
Regenerated Plants ◽  
Indirect Somatic Embryogenesis ◽  
Carum Copticum

scholarly journals Optimized somatic embryogenesis and plant regeneration in elite Argentinian sugarcane (Saccharum spp.) cultivars

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Valentina Di Pauli ◽  
Paola Daniela Fontana ◽  
Dalia Marcela Lewi ◽  
Arturo Felipe ◽  
Luis Ernesto Erazzú
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Culture Conditions ◽  
Light Conditions ◽  
Saccharum Spp ◽  
Regenerated Plants ◽  
Embryogenic Calluses ◽  
Indirect Somatic Embryogenesis ◽  
Good Number

Abstract Background Biotechnological breeding of elite sugarcane cultivars is currently limited because of the difficulty of regenerating plants by tissue culture. Here, we report that commercially elite sugarcane genotypes, which are adapted to Argentinian agro-ecological conditions, are capable of being regenerated via indirect somatic embryogenesis. Leaf rolls of five elite genotypes were cultured following two callus induction protocols using different concentrations of 2,4-D as the growth regulator. Embryogenic calluses were regenerated under light conditions. Regenerated plants were subsequently acclimatized in the greenhouse under two acclimatization procedures before being transplanted to the field. Results Four of the five genotypes were able to form somatic embryos following the two induction protocols. The variables related to embryogenic callus production were influenced by the interaction between genotype and culture conditions. For plant regeneration, the embryogenic calluses were further cultured on an IBA-supplemented medium, where we observed a high genotype dependence. Calluses from the four cultivars regenerated a good number of plants. With the procedures described here, we obtained more than 90% of well-acclimatized plants both in the greenhouse and in the field. Conclusions This protocol provides a simple way to regenerate sugarcane plants through indirect somatic embryogenesis. Also, the results confirm that tissue culture ability is highly genotype-dependent in sugarcane. Our findings suggest that these elite cultivars could be good candidates for biotechnological breeding.

scholarly journals Protocol development for somatic embryogenesis, SSR markers and genetic modification of Stipagrostis pennata (Trin.) De Winter

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Masoumeh Asadi-Aghbolaghi ◽  
Beata Dedicova ◽  
Sonali Sachi Ranade ◽  
Kim-Cuong Le ◽  
Farzad Sharifzadeh ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Somaclonal Variation ◽  
Ssr Markers ◽  
Genetic Stability ◽  
Somatic Embryos ◽  
Large Scale ◽  
Scale Production ◽  
Ms Medium ◽  
Important Species ◽  
Regenerated Plants

Abstract Background Stipagrostis pennata (Trin.) De Winter is an important species for fixing sand in shifting and semi-fixed sandy lands, for grazing, and potentially as a source of lignocellulose fibres for pulp and paper industry. The seeds have low viability, which limits uses for revegetation. Somatic embryogenesis offers an alternative method for obtaining large numbers of plants from limited seed sources. Results A protocol for plant regeneration from somatic embryos of S. pennata was developed. Somatic embryogenesis was induced on Murashige & Skoog (MS) medium supplemented with 3 mg·L–1 2,4-D subsequently shoots were induced on MS medium and supplemented with 5 mg·L–1 zeatin riboside. The highest shoots induction was obtained when embryogenic callus derived from mature embryos (96%) in combination with MS filter-sterilized medium was used from Khuzestan location. The genetic stability of regenerated plants was analysed using ten simple sequence repeats (SSR) markers from S. pennata which showed no somaclonal variation in regenerated plants from somatic embryos of S. pennata. The regenerated plants of S. pennata showed genetic stability without any somaclonal variation for the four pairs of primers that gave the expected amplicon sizes. This data seems very reliable as three of the PCR products belonged to the coding region of the genome. Furthermore, stable expression of GUS was obtained after Agrobacterium-mediated transformation using a super binary vector carried by a bacterial strain LBA4404. Conclusion To our knowledge, the current work is the first attempt to develop an in vitro protocol for somatic embryogenesis including the SSR marker analyses of regenerated plants, and Agrobacterium-mediated transformation of S. pennata that can be used for its large-scale production for commercial purposes.

Attempts to eradicate graft-transmissible infections through somatic embryogenesis in Citrus ssp. and analysis of genetic stability of regenerated plants

2016 ◽  
Vol 148 (1) ◽  
pp. 85-95 ◽  
Author(s):  
Malika Meziane ◽  
Dajana Frasheri ◽  
Angela Carra ◽  
Messaoud Boudjeniba ◽  
Anna Maria D’Onghia ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Stability ◽  
Regenerated Plants

scholarly journals Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbata

2020 ◽  
Vol 64 ◽  
pp. 224-233
Author(s):  
A. CHAVEZ-CORTAZAR ◽  
M. MATA-ROSAS ◽  
K. OYAMA ◽  
M.S. SAMAIN ◽  
M. QUESADA
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Stability ◽  
Regenerated Plants ◽  
Magnolia Dealbata

scholarly journals A SIMPLE PROTOCOL FOR SOMATIC EMBRYOGENESIS INDUCTION OF IN VITRO SUGARCANE ( Saccharum officinarum. L) BY 2,4-D AND BAP

2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Laily Ilman Widuri ◽  
Parawita Dewanti ◽  
Bambang Sugiharto
Keyword(s):  
Somatic Embryogenesis ◽  
Shoot Regeneration ◽  
Callus Induction ◽  
Saccharum Officinarum ◽  
Randomized Design ◽  
Indirect Somatic Embryogenesis ◽  
Simple Protocol ◽  
Callus Proliferation ◽  
Completely Randomized Design

Induction of in vitro sugarcane through somatic embryogenesis technique influenced by addition of plant growth regulator. The objective of this research was to determine appropriate formulation medium for indirect somatic embryogenesis induction on two potential sugarcane SUT Event 02 and PS 881. This research carried out in three steps, callus induction, callus proliferation, and shoot regeneration. Explants taken from basal of in vitro plantlet one month SUT Event 02 and PS 881 resulted from shoot regeneration previously. Five different medium formulas applied for callus induction and one formula for proliferation and shoot regeneration. Research using completely randomized design (CRD) factorial with five different formulation induction mediums. The result showed that the best respond of indirect somatic embryogenesis on SUT Event 02 and PS 881 was medium containing  3 mgL-1of  2,4-D.

Tissue culture of saffron (Crocus sativusL.): Somatic embryogenesis and shoot regeneration

1992 ◽  
Vol 6 (3) ◽  
pp. 217-223 ◽  
Author(s):  
P.S. George ◽  
Sujata Visvanath ◽  
G.A. Ravishankar ◽  
L.V. Venkataraman
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Shoot Regeneration

scholarly journals Influence of Abscisic Acid and Sucrose on Somatic Embryogenesis in CactusCopiapoa tenuissimaRitt. formamostruosa

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
J. Lema-Rumińska ◽  
K. Goncerzewicz ◽  
M. Gabriel
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Somatic Embryos ◽  
Sucrose Concentration ◽  
Turgor Pressure ◽  
Direct Somatic Embryogenesis ◽  
Fresh Weight ◽  
High Concentration ◽  
Globular Stage ◽  
Indirect Somatic Embryogenesis

Having produced the embryos of cactusCopiapoa tenuissimaRitt. formamonstruosaat the globular stage and callus, we investigated the effect of abscisic acid (ABA) in the following concentrations: 0, 0.1, 1, 10, and 100 μM on successive stages of direct (DSE) and indirect somatic embryogenesis (ISE). In the indirect somatic embryogenesis process we also investigated a combined effect of ABA (0, 0.1, 1 μM) and sucrose (1, 3, 5%). The results showed that a low concentration of ABA (0-1 μM) stimulates the elongation of embryos at the globular stage and the number of correct embryos in direct somatic embryogenesis, while a high ABA concentration (10–100 μM) results in growth inhibition and turgor pressure loss of somatic embryos. The indirect somatic embryogenesis study in this cactus suggests that lower ABA concentrations enhance the increase in calli fresh weight, while a high concentration of 10 μM ABA or more changes calli color and decreases its proliferation rate. However, in the case of indirect somatic embryogenesis, ABA had no effect on the number of somatic embryos and their maturation. Nevertheless, we found a positive effect of sucrose concentration for both the number of somatic embryos and the increase in calli fresh weight.

Sign in / Sign up

Export Citation Format

Share Document