scholarly journals Comparative analysis of nitrogen metabolism-related genes in two inbred maize lines with different low-nitrogen tolerance

2020 ◽  
Vol 64 ◽  
pp. 289-301
Author(s):  
M. YAN ◽  
J. LUO ◽  
L. LIANG ◽  
L. CHEN ◽  
Y.Y. CAO ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Nitrogen Metabolism ◽  
Low Nitrogen Tolerance ◽  
Low Nitrogen

scholarly journals Expression Analysis of Nitrogen Metabolism-Related Genes Reveals Differences in Adaptation to Low-Nitrogen Stress between Two Different Barley Cultivars at Seedling Stage

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Zhiwei Chen ◽  
Chenghong Liu ◽  
Yifei Wang ◽  
Ting He ◽  
Runhong Gao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitrogen Metabolism ◽  
Expression Analysis ◽  
Nitrogen Fertilizers ◽  
Nitrogen Stress ◽  
Environmental Damage ◽  
Barley Cultivars ◽  
Low Nitrogen Stress ◽  
Low Nitrogen Tolerance ◽  
Low Nitrogen

The excess use of nitrogen fertilizers causes many problems, including higher costs of crop production, lower nitrogen use efficiency, and environmental damage. Crop breeding for low-nitrogen tolerance, especially molecular breeding, has become the major route to solving these issues. Therefore, in crops such as barley (Hordeum vulgare L.), it is crucial to understand the mechanisms of low-nitrogen tolerance at the molecule level. In the present study, two barley cultivars, BI-04 (tolerant to low nitrogen) and BI-45 (sensitive to low nitrogen), were used for gene expression analysis under low-nitrogen stress, including 10 genes related to primary nitrogen metabolism. The results showed that the expressions of HvNIA2 (nitrite reductase), HvGS2 (chloroplastic glutamine synthetase), and HvGLU2 (ferredoxin-dependent glutamate synthase) were only induced in shoots of BI-04 under low-nitrogen stress, HvGLU2 was also only induced in roots of BI-04, and HvGS2 showed a rapid response to low-nitrogen stress in the roots of BI-04. The expression of HvASN1 (asparagine synthetase) was reduced in both cultivars, but it showed a lower reduction in the shoots of BI-04. In addition, gene expression and regulation differences in the shoots and roots were also compared between the barley cultivars. Taken together, the results indicated that the four above-mentioned genes might play important roles in low-nitrogen tolerance in barley.

scholarly journals Evaluation of Yield-Based Low Nitrogen Tolerance Indices for Screening Maize (Zea mays L.) Inbred Lines

Agronomy ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 240 ◽  
Author(s):  
Zhixin Zhao ◽  
Kunhui He ◽  
Zhiqian Feng ◽  
Yanan Li ◽  
Liguo Chang ◽  
...  
Keyword(s):  
Grain Yield ◽  
Geometric Mean ◽  
Inbred Lines ◽  
Shaanxi Province ◽  
High Yield ◽  
Tolerance Index ◽  
Heterotic Groups ◽  
Maize Inbred Lines ◽  
Low Nitrogen Tolerance ◽  
Low Nitrogen

To screen the desired criterion to identify desirable genotypes and select genotypes best suited to limited nitrogen availability in order to facilitate the practice of low-nitrogen-tolerant breeding in maize, the response of 31 maize inbred lines, containing four control inbred lines (PH6WC, PH4CV, Zheng58, and Chang7-2) and others selected from the Shaan A and Shaan B heterotic groups cultivated at Northwest A&F University (Yangling, Shaanxi, China), were evaluated. The experiment was conducted following a split plot design with two replications during three growing seasons (2015, 2016, and 2017) under both high nitrogen (HN) and low nitrogen (LN) conditions at the Yulin and Yangling in Shaanxi Province, China. Seven screening indices, based on grain yield under two contrasting nitrogen (N) conditions, the stress susceptibility index (SSI), yield stability index (YSI), mean productivity (MP), geometric mean productivity (GMP), stress tolerance index (STI), harmonic mean (HM), and low nitrogen tolerance index (LNTI), were computed to assess the overall index that accurately screened the desirable genotypes. The results of the correlation analyses and principal component analysis showed that MP, GMP, HM and STI were correlated with grain yield significantly and positively under contrasting N conditions, and were able to accurately discriminate the desirable genotypes. Compared with the control inbred lines, many inbred lines selected from the Shaan A and Shaan B groups showed a higher LN tolerance. This shows that we can effectively improve the LN tolerance of maize inbred lines through LN screening. Based on the screening indices, the three-dimensional diagram and genotype and genotype × environment (GGE) biplots are agreed with this results, and we identified KA105, KB081, KA225, 91227, and 2013KB-47 as the desired genotypes that have the potential to be used to breed a high yield and stable hybrid.

QTL mapping for seedling traits associated with low-nitrogen tolerance using a set of advanced backcross introgression lines of rice

2013 ◽  
Vol 133 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Chun-Fang Zhao ◽  
Li-Hui Zhou ◽  
Ya-Dong Zhang ◽  
Zhen Zhu ◽  
Tao Chen ◽  
...  
Keyword(s):  
Qtl Mapping ◽  
Introgression Lines ◽  
Seedling Traits ◽  
Advanced Backcross ◽  
Low Nitrogen Tolerance ◽  
Low Nitrogen

scholarly journals Population structure analysis and identification of genomic regions under selection associated with low-nitrogen tolerance in tropical maize lines

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0239900
Author(s):  
Gustavo César Sant’Ana ◽  
Fernando Garcia Espolador ◽  
Ítalo Stefanine Correia Granato ◽  
Leandro Freitas Mendonça ◽  
Roberto Fritsche-Neto ◽  
...  
Keyword(s):  
Population Structure ◽  
Structure Analysis ◽  
Tropical Maize ◽  
Population Structure Analysis ◽  
Genomic Regions ◽  
Low Nitrogen Tolerance ◽  
Low Nitrogen

scholarly journals Transcriptomic and Metabolomics Analysis of Different Endosperm Region under Nitrogen Treatments

2019 ◽  
Vol 20 (17) ◽  
pp. 4212 ◽  
Author(s):  
Dongyun Ma ◽  
Honghuan Gao ◽  
Chenyang Du ◽  
Lingli Li ◽  
Wan Sun ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Nitrogen Metabolism ◽  
Protein Distribution ◽  
Post Translational Modification ◽  
Expression Change ◽  
Heterogeneous Distribution ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Metabolism ◽  
Nitrogen Treatments ◽  
Low Nitrogen

Storage protein distribution in wheat-grain endosperm is heterogeneous, but the underlying molecular mechanism remains unclear. Two parts of the endosperm region, the innermost endosperm (IE) region and the remaining endosperm (RE) region, grown under low nitrogen (LN) and high nitrogen (HN) treatments were used to perform metabolomic and transcriptomic analysis. We identified 533 and 503 differentially expressed genes (DEGs) with at least a two-fold expression change (p < 0.05) between IE and RE, among which 81 and 78 transcripts under LN and HN, respectively, related to carbon and nitrogen metabolism, and encoded transcription factors or proteins involved in post-translational modification (PTM). The significantly differentially abundant metabolites between IE and RE were mainly amino acids, N-compounds, carbohydrates, and nucleic acids. More upregulated transcripts and metabolites were identified in RE than IE under HN conditions, indicating that HN activates metabolism in the endosperm periphery. In addition to carbon and nitrogen metabolism, transcription factors and protein PTMs, such as phosphorylation and acetylation, might determine the protein heterogeneous distribution between IE and RE and its response to nitrogen fertilizer supply.

The effect of increased rectal temperature on nitrogen metabolism in Brahman cross and Shorthorn × Hereford steers fed on a low nitrogen roughage

1970 ◽  
Vol 21 (5) ◽  
pp. 857 ◽  
Author(s):  
JE Vercoe ◽  
JE Frisch
Keyword(s):  
Urinary Excretion ◽  
Nitrogen Metabolism ◽  
Rectal Temperature ◽  
Water Losses ◽  
Fixed Amount ◽  
Evaporative Water ◽  
Urea Nitrogen ◽  
Dry Matter Digestibility ◽  
Collection Period ◽  
Low Nitrogen

A study was made of the effect of increasing the rectal temperature on nitrogen metabolism in Brahman cross and British cross steers offered a fixed amount of a low nitrogen roughage. Increasing the rectal temperature by c. 1.3�C in either breed slightly depressed intake, increased the dry matter digestibility by a small but statistically significant amount, and had no effect on the nitrogen digestibility. It did not significantly increase the urinary excretion of total nitrogen or urea nitrogen over the whole collection period, but over the last 5 days there were increases in the urinary total and urea nitrogen excreted per day which in most cases were significant. Increasing the rectal temperature significantly increased the urinary excretion of creatinine. The ambient temperature at which rectal temperatures were increased by 1.3�C was 35�C for the British cross steers and 43' for the Brahman cross steers. At the increased rectal temperature, the Brahman cross steers drank significantly more water than the British cross steers although faecal and urinary water losses were not significantly different. The calculated evaporative water loss was higher in the Brahman cross steers than in the British cross steers when both breeds were heated.

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