scholarly journals Biochemical examination of non-transgenic and transgenic soybean plants under drought stress conditions

2019 ◽  
Vol 63 (1) ◽  
pp. 314-322
Author(s):  
C.G. Goncalves ◽  
A.C. Silva ◽  
L.R. Alves ◽  
M.R.R. Pereira ◽  
P.L. Gratao ◽  
...  
Keyword(s):  
Drought Stress ◽  
Stress Conditions ◽  
Transgenic Soybean ◽  
Soybean Plants ◽  
Biochemical Examination

scholarly journals Overexpression of the GmDREB2 gene increases proline accumulation and tolerance to drought stress in soybean plants

2020 ◽  
pp. 495-503
Author(s):  
Thi Thanh Nhan Pham ◽  
Huu Quan Nguyen ◽  
Thi Ngoc Lan Nguyen ◽  
Xuan Tan Dao ◽  
Danh Thuong Sy ◽  
...  
Keyword(s):  
Glycine Max ◽  
Drought Stress ◽  
Transgenic Plants ◽  
Proline Accumulation ◽  
Stress Conditions ◽  
Transgenic Soybean ◽  
Transcription Level ◽  
Drought Tolerant ◽  
Transgenic Lines ◽  
Soybean Plants

The dehydration responsive element binding (DREB) is a plant protein subfamily expressed when soybean plants face abiotic stresses. These DREB proteins are also considered to activate the transcription of drought-resistant genes. In this study, we present the determined results of relationships between overexpression of Glycine max DREB2 (GmDREB2) with the transcription level of Glycine max pyrroline-5-carboxylate synthetase (GmP5CS) gene, proline accumulation and drought tolerant ability transgenic soybean plants as the basis for selection of transgenic lines with high drought tolerance. GmDREB2 was inserted into a plant transgenic vector and the 35S-GmDREB2-cmyc construct was transferred into the soybean plants by Agrobacterium-mediated transformation. Recombinant GmDREB2 protein with a molecular weight of approximately 20 kDa was expressed in four transgenic soybean lines in the T1 generation. The GmP5CS gene was shown to have significantly (P<0.05) increased expression in the T2 transgenic soybean lines and higher than compared to non-transgenic plants with considering both in well watered condition and stressed conditions were from 1.06 to 1.31-fold and 1.37 to 1.95-fold, respectively. The proline content of four transgenic soybean lines increased 155.81% to 187.42 % after five days in drought-stress conditions and increased from 180.52 % to 233.74 % after nine days under drought-stress conditions (P<0.05). Therefore, the overexpression of GmDREB2 resulted in increasing transcription level of P5CS gene, proline accumulation and drought-stress tolerance of the transgenic soybean plants. The GmDREB2 transformation into soybean plants was confirmed by the results of genetically modified lines in the T2 generation (T2-1, T2-6, T2-7, and T2-8) with higher drought tolerant ability than those of non-transgenic plants.

Photosynthetic response of transgenic soybean plants, containing an Arabidopsis P5CR gene, during heat and drought stress

2004 ◽  
Vol 161 (11) ◽  
pp. 1211-1224 ◽  
Author(s):  
J.A. De Ronde ◽  
W.A. Cress ◽  
G.H.J. Krüger ◽  
R.J. Strasser ◽  
J. Van Staden
Keyword(s):  
Drought Stress ◽  
Photosynthetic Response ◽  
Transgenic Soybean ◽  
Soybean Plants

The GmXTH1 Gene Improves Drought Stress Resistance of Soybean Seedlings

2021 ◽  
Author(s):  
Ye Zhang ◽  
Han-zhu Zhang ◽  
Jia-yu Fu ◽  
Ye-yao Du ◽  
Jing Qu ◽  
...  
Keyword(s):  
Drought Stress ◽  
Water Content ◽  
Germination Rate ◽  
Stress Conditions ◽  
Germination Index ◽  
Root Tip ◽  
Projection Area ◽  
Transgenic Soybean ◽  
Control Material ◽  
Germination Potential

Abstract To study the role of GmXTH1 gene in alleviating drought stress. T4 transgenic soybean seeds with GmXTH1 gene were treated with PEG6000 at 0%, 5%, 10% and 15%, respectively. Germination potential, germination rate and germination index were measured. The results showed that the germination potential, germination rate and germination index of OEA1 and OEA2 strains overexpressed in T4 generation were significantly higher than that of control material M18. After 0d, 7d and 15d drought stress, the analysis of seedling phenotypes and root-shoot of different T4 generation transgenic soybean lines showed that under stress conditions, the growth of GmXTH1 overexpression material was generally better than that of control material M18, and the growth of GmXTH1 interference expression material was generally worse than that of control material M18, with significant differences in plant phenotypes.The root system of GmXTH1 overexpressed material was significantly developed compared with that of control material M18. The analysis of physiological and biochemical indexes showed that the relative water content and the activity of antioxidant enzymes (superoxide dismutase and peroxidase) of GmXTH1 transgenic soybean material were significantly higher than that of control material M18, and the accumulation of malondialdehyde was lower under the same stress conditions at seedling stage. Fluorescence quantitative PCR assay showed that the relative expression of GmXTH1 gene in transgenic soybean was significantly increased after drought stress.The results showed that the overexpression of GmXTH1 could increase the total root length, surface area, total projection area, root volume, average diameter, total cross number and total root tip number, thereby increasing the water intake and reducing the transpiration of water content in leaves, thus reducing the accumulation of MDA and producing more protective enzymes in a more effective and prompt way.Reducing cell membrane damage to improve drought resistance of soybean.

scholarly journals Physiomorphology of Soybean as Affected by Drought Stress and Nitrogen Application

Scientifica ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Oqba Basal ◽  
András Szabó
Keyword(s):  
Drought Stress ◽  
Fertilizer Application ◽  
N Fertilizer ◽  
Stress Conditions ◽  
The Other ◽  
Nitrogen Application ◽  
Total N ◽  
Affected Plant ◽  
Soybean Plants ◽  
N Demand

Drought periods are predicted to increase in the future, putting the production of sensitive crops under serious hazards. Soybean, as a legume, is capable of partly achieving its nitrogen demands through the N2-fixation process; however, this process is inhibited by drought stress conditions. Moreover, N2-fixation might not fulfill the total N demand for soybean plants, so supplemental N-fertilizer doses might be crucial. A 3-year experiment was carried out in Debrecen, Hungary, to investigate the effects of inoculation and N-fertilizer application on the physiomorphology of soybean (cv. Boglár) under both drought stress and irrigated conditions. Results showed that, regardless of inoculation, drought negatively affected plant height, LAI, SPAD, and, to a smaller extent, NDVI. On average, increasing N-fertilizer enhanced these traits accordingly. Inoculation, on the other hand, resulted in taller plants and higher LAI values, but lower SPAD values. It could be concluded that soybean’s physiomorphology is negatively influenced by drought stress and that N-fertilizer application can enhance it whether soybean plants suffer from drought stress conditions or not.

Flavonoid profiling by LC-MS IN 14 – 3-3ν knockout mutants of Arabidopsis thaliana during drought stress conditions

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
F Nabbie ◽  
O Shperdheja ◽  
J Millot ◽  
J Lindberg ◽  
B Peethambaran
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Stress Conditions ◽  
Knockout Mutants

scholarly journals Physiological and biochemical responses of soybean plants inoculated with Arbuscular mycorrhizal fungi and Bradyrhizobium under drought stress

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mohamed S. Sheteiwy ◽  
Dina Fathi Ismail Ali ◽  
You-Cai Xiong ◽  
Marian Brestic ◽  
Milan Skalicky ◽  
...  
Keyword(s):  
Drought Stress ◽  
Arbuscular Mycorrhizal Fungi ◽  
Secondary Metabolism ◽  
Mycorrhizal Fungi ◽  
Plant Biomass ◽  
Arbuscular Mycorrhizal ◽  
Growth And Yield ◽  
Relative Expression ◽  
Reverse Trend ◽  
Soybean Plants

Abstract Background The present study aims to study the effects of biofertilizers potential of Arbuscular Mycorrhizal Fungi (AMF) and Bradyrhizobium japonicum (B. japonicum) strains on yield and growth of drought stressed soybean (Giza 111) plants at early pod stage (50 days from sowing, R3) and seed development stage (90 days from sowing, R5). Results Highest plant biomass, leaf chlorophyll content, nodulation, and grain yield were observed in the unstressed plants as compared with water stressed-plants at R3 and R5 stages. At soil rhizosphere level, AMF and B. japonicum treatments improved bacterial counts and the activities of the enzymes (dehydrogenase and phosphatase) under well-watered and drought stress conditions. Irrespective of the drought effects, AMF and B. japonicum treatments improved the growth and yield of soybean under both drought (restrained irrigation) and adequately-watered conditions as compared with untreated plants. The current study revealed that AMF and B. japonicum improved catalase (CAT) and peroxidase (POD) in the seeds, and a reverse trend was observed in case of malonaldehyde (MDA) and proline under drought stress. The relative expression of the CAT and POD genes was up-regulated by the application of biofertilizers treatments under drought stress condition. Interestingly a reverse trend was observed in the case of the relative expression of the genes involved in the proline metabolism such as P5CS, P5CR, PDH, and P5CDH under the same conditions. The present study suggests that biofertilizers diminished the inhibitory effect of drought stress on cell development and resulted in a shorter time for DNA accumulation and the cycle of cell division. There were notable changes in the activities of enzymes involved in the secondary metabolism and expression levels of GmSPS1, GmSuSy, and GmC-INV in the plants treated with biofertilizers and exposed to the drought stress at both R3 and R5 stages. These changes in the activities of secondary metabolism and their transcriptional levels caused by biofertilizers may contribute to increasing soybean tolerance to drought stress. Conclusions The results of this study suggest that application of biofertilizers to soybean plants is a promising approach to alleviate drought stress effects on growth performance of soybean plants. The integrated application of biofertilizers may help to obtain improved resilience of the agro ecosystems to adverse impacts of climate change and help to improve soil fertility and plant growth under drought stress.

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