scholarly journals Diagnosis of Maedi-Visna in Dairy Sheep

2020 ◽  
Author(s):  
Aphrodite I. Kalogianni
Keyword(s):  
Horizontal Transmission ◽  
Viral Disease ◽  
Research Field ◽  
Diagnostic Methods ◽  
Dairy Sheep ◽  
Diagnostic Assays ◽  
Control Programs ◽  
Effective Strategies ◽  
Worldwide Distribution ◽  
Evidential Basis

Maedi-visna in sheep is a chronic viral disease with worldwide distribution causing significant monetary losses in the sheep sector. Lack of effective treatment or vaccines, knowledge gaps regarding the epizootiology of the disease (e.g. significance of vertical and horizontal transmission) and the scarcity of protocols for the early and efficient diagnosis of maedi-visna infections are the major obstacles in the implementation of universally accepted control programs. The diagnostic methods currently used are based on the detection of either antibodies or the viral genome. In general, accuracy, sensitivity and specificity of the available serological and molecular assays vary and therefore, modifications on the diagnostic protocols on an evidential basis are necessary. Therefore, the combination of diagnostic assays and the development of effective strategies for mass implementation thereof are critical targets to achieve and constitute major challenges in maedi-visna research field.

scholarly journals Immunopathogenic and Neurological Mechanisms of Canine Distemper Virus

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Otávio Valério Carvalho ◽  
Clarisse Vieira Botelho ◽  
Caroline Gracielle Torres Ferreira ◽  
Paulo Oldemar Scherer ◽  
Jamária Adriana Pinheiro Soares-Martins ◽  
...  
Keyword(s):  
Canine Distemper Virus ◽  
Clinical Manifestations ◽  
Specific Treatment ◽  
Viral Disease ◽  
Diagnostic Methods ◽  
Lymphoid Tissues ◽  
Canine Distemper ◽  
Worldwide Distribution ◽  
Natural Hosts ◽  
The Central Nervous System

Canine distemper is a highly contagious viral disease caused by the canine distemper virus (CDV), which is a member of theMorbillivirusgenus, Paramyxoviridae family. Animals that most commonly suffer from this disease belong to the Canidae family; however, the spectrum of natural hosts for CDV also includes several other families of the order Carnivora. The infectious disease presents worldwide distribution and maintains a high incidence and high levels of lethality, despite the availability of effective vaccines, and no specific treatment. CDV infection in dogs is characterized by the presentation of systemic and/or neurological courses, and viral persistence in some organs, including the central nervous system (CNS) and lymphoid tissues. An elucidation of the pathogenic mechanisms involved in canine distemper disease will lead to a better understanding of the injuries and clinical manifestations caused by CDV. Ultimately, further insight about this disease will enable the improvement of diagnostic methods as well as therapeutic studies.

A rapid method for the direct identification of paramyxovirus in canine CSF by ultracentrifugation and negative staining as a rule out for distemper

1990 ◽  
Vol 48 (3) ◽  
pp. 594-595
Author(s):  
W.L. Steffens ◽  
M.B. Ard ◽  
C.E. Greene ◽  
A. Jaggy
Keyword(s):  
Subacute Sclerosing Panencephalitis ◽  
Clinical Signs ◽  
Viral Disease ◽  
Etiologic Agent ◽  
Mucosal Inflammation ◽  
Worldwide Distribution ◽  
Negative Stain ◽  
Viral Particles ◽  
Systemic Manifestations ◽  
Rule Out

Canine distemper is a multisystemic contagious viral disease having a worldwide distribution, a high mortality rate, and significant central neurologic system (CNS) complications. In its systemic manifestations, it is often presumptively diagnosed on the basis of clinical signs and history. Few definitive antemortem diagnostic tests exist, and most are limited to the detection of viral antigen by immunofluorescence techniques on tissues or cytologic specimens or high immunoglobulin levels in CSF (cerebrospinal fluid). Diagnosis of CNS distemper is often unreliable due to the relatively low cell count in CSF (<50 cells/μl) and the binding of blocking immunoglobulins in CSF to cell surfaces. A more reliable and definitive test might be possible utilizing direct morphologic detection of the etiologic agent. Distemper is the canine equivalent of human measles, in that both involve a closely related member of the Paramyxoviridae, both produce mucosal inflammation, and may produce CNS complications. In humans, diagnosis of measles-induced subacute sclerosing panencephalitis is through negative stain identification of whole or incomplete viral particles in patient CSF.

Current Prevalence and Treatment Outcome of Paediatric Tuberculosis in a South Indian District - A 2 Year Retrospective Observational Study

2021 ◽  
Vol 8 (25) ◽  
pp. 2229-2234
Author(s):  
Praveen Deen Kumar Namala ◽  
Praveena Basireddy
Keyword(s):  
Drug Resistance ◽  
Treatment Outcome ◽  
Favourable Outcome ◽  
Diagnostic Methods ◽  
Resistance Pattern ◽  
Control Programs ◽  
South Indian ◽  
Current Prevalence ◽  
Drug Resistance Pattern ◽  
Paediatric Tuberculosis

BACKGROUND Knowledge about the diagnosis and the treatment outcome of paediatric tuberculosis is very much essential for functioning of tuberculosis (TB) control programs in most countries like India. The current study was done to determine the prevalence of paediatric tuberculosis, different modalities used for diagnosis, drug resistance pattern, HIV-TB co-infection rate and treatment outcome. METHODS A 2-year retrospective study was done among all registered paediatric tuberculosis cases aged 18 years and below, who were diagnosed during the period January 2018 to December 2019. Data was extracted from the files and was analysed. RESULTS The prevalence of paediatric tuberculosis was found to be 0.51 per 1000 population. The proportion of paediatric tuberculosis among total TB cases was 5.9 %. Among 14,596 total TB cases registered at District Tuberculosis Centre (DTC) Anantapuramu, 867 (5.9 %) were paediatric TB cases, with a mean age of 12.5 ± 5.6 years. 62 % of the cases were above 12 years of age. Males (47.4 %) and females (52.6 %) were almost equally distributed (P > 0.05). 343 (39.6 %) cases were microbiologically confirmed TB and 524 (60.4 %) cases were clinically diagnosed TB. Drug resistance was found in 47 (13.7 %) of the 343 microbiologically confirmed TB cases. Of the total 867 paediatric TB cases studied, pulmonary type (56 %) was predominant than extra pulmonary type (44 %) (P < 0.05). Human immunodeficiency virus (HIV) co infection was found in 24 (2.8 %) cases. 423 cases (65.5 %) successfully completed the treatment and 173 cases (26.8 %) were cured adding to a favourable outcome of 92.3 %. 3 (0.5 %) cases had treatment failure, 35 (5.4 %) were defaulters and 12 (1.8 %) cases died during the treatment, adding to a poor outcome of about 7.7 %. CONCLUSIONS Majority of the paediatric tuberculosis cases were diagnosed clinically rather than microbiological confirmation. Efforts should be made to further improve the availability and sensitivity of diagnostic methods of paediatric TB. KEYWORDS Paediatric Tuberculosis, Drug Resistant TB, HIV TB Co Infection

scholarly journals “Study Natural” without Drugs: An Exploratory Study of Theory-Guided and Tailored Health Campaign Interventions to Prevent Nonmedical Use of Prescription Stimulants in College Students

2020 ◽  
Vol 17 (12) ◽  
pp. 4421
Author(s):  
Zhaohui Su ◽  
Michael Mackert ◽  
Xiaoshan Li ◽  
Jiyoon (Karen) Han ◽  
Brittani Crook ◽  
...  
Keyword(s):  
College Students ◽  
Online Survey ◽  
Research Field ◽  
Health Campaign ◽  
Prescription Stimulants ◽  
Effective Strategies ◽  
Nonmedical Use ◽  
Students Attitudes ◽  
And Behavior ◽  
Attitudes And Behavior

Nonmedical use of prescription stimulants (NMUPS) among college students continues to rise. While some anti-NMUPS campaigns are available, little is known about the campaign development process and how well college students evaluate these messages. To bridge this gap, we developed theory-guided anti-NMUPS campaign interventions that are tailored to college students’ characteristics and evaluated students’ response towards them. A total of 445 college students (74.4% female; Meanage of 20; 18 to 35 years old) reviewed the campaign interventions and offered their evaluation via an online survey. Findings indicate that students responded to the campaigns positively. Results also indicate that female students are more likely to perceive the campaigns as effective than their male counterparts. Overall, the findings of this study suggest that theory-guided and tailored anti-NMUPS campaigns have great potential in changing students’ attitudes and behavior towards NMUPS. While this study fills critical gaps in the literature, considering the progress needed to strengthen the research field, more research is needed to further identify effective strategies that could prevent college students’ participation in NMUPS activities.

scholarly journals Detection of Cryptosporidium and Giardia in foods of plant origin in North-Western Greece

2020 ◽  
Vol 18 (4) ◽  
pp. 574-578
Author(s):  
Hercules Sakkas ◽  
Vangelis Economou ◽  
Petros Bozidis ◽  
Panagiota Gousia ◽  
Chrissanthy Papadopoulou ◽  
...  
Keyword(s):  
Health Hazard ◽  
Diarrhoeal Disease ◽  
Diagnostic Methods ◽  
Plant Origin ◽  
Potential Health ◽  
Worldwide Distribution ◽  
Fresh Vegetables ◽  
Vegetables And Fruits ◽  
North Western ◽  
Western Greece

Abstract Giardia and Cryptosporidium are recognized as leading causes of waterborne and foodborne diarrhoeal disease with worldwide distribution. The study aimed to determine the protozoan contamination of various foods of plant origin. A total of 72 samples from 27 different varieties of fresh vegetables and fruits were collected from supermarkets and open markets in North-Western Greece and were examined using conventional diagnostic methods. Two out of 72 (2.8%) samples were found positive for Cryptosporidium oocysts, while no sample was found to be positive for Giardia cysts. The results show the presence of protozoan contamination in foods of plant origin, which may constitute a potential health hazard.

scholarly journals Enhancing colorimetric loop-mediated isothermal amplification speed and sensitivity with guanidine chloride

BioTechniques ◽  
2020 ◽  
Vol 69 (3) ◽  
pp. 178-185 ◽  
Author(s):  
Yinhua Zhang ◽  
Guoping Ren ◽  
Jackson Buss ◽  
Andrew J Barry ◽  
Gregory C Patton ◽  
...  
Keyword(s):  
Guanidine Hydrochloride ◽  
Isothermal Amplification ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Simple Method ◽  
Diagnostic Assays ◽  
Loop Mediated Isothermal Amplification ◽  
Dna And Rna ◽  
Versatile Technique ◽  
Primer Sets

Loop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. The global SARS-CoV-2 pandemic has presented an urgent need for new and better diagnostic methods, with colorimetric LAMP utilized in numerous studies for SARS-CoV-2 detection. However, the sensitivity of colorimetric LAMP in early reports has been below that of the standard RT-qPCR tests, and we sought to improve performance. Here we report the use of guanidine hydrochloride and combined primer sets to increase speed and sensitivity in colorimetric LAMP, bringing this simple method up to the standards of sophisticated techniques and enabling accurate, high-throughput diagnostics.

scholarly journals Genome-health nutrigenomics and nutrigenetics: nutritional requirements or ‘nutriomes’ for chromosomal stability and telomere maintenance at the individual level

2008 ◽  
Vol 67 (2) ◽  
pp. 146-156 ◽  
Author(s):  
Caroline Bull ◽  
Michael Fenech
Keyword(s):  
Research Field ◽  
Telomere Maintenance ◽  
Health Maintenance ◽  
Individual Level ◽  
Chromosomal Stability ◽  
Effective Strategies ◽  
Health Clinics ◽  
Genome Damage ◽  
Health Strategy ◽  
The Individual

It is becoming increasingly evident that (a) risk for developmental and degenerative disease increases with more DNA damage, which in turn is dependent on nutritional status, and (b) the optimal concentration of micronutrients for prevention of genome damage is also dependent on genetic polymorphisms that alter the function of genes involved directly or indirectly in the uptake and metabolism of micronutrients required for DNA repair and DNA replication. The development of dietary patterns, functional foods and supplements that are designed to improve genome-health maintenance in individuals with specific genetic backgrounds may provide an important contribution to an optimum health strategy based on the diagnosis and individualised nutritional prevention of genome damage, i.e. genome health clinics. The present review summarises some of the recent knowledge relating to micronutrients that are associated with chromosomal stability and provides some initial insights into the likely nutritional factors that may be expected to have an impact on the maintenance of telomeres. It is evident that developing effective strategies for defining nutrient doses and combinations or ‘nutriomes’ for genome-health maintenance at the individual level is essential for further progress in this research field.

scholarly journals Systemic and local immune mechanisms stimulated in the course of chicken infectious bronchitis

2016 ◽  
Vol 72 (6) ◽  
pp. 358-363
Author(s):  
Marcin Śmiałek ◽  
Joanna Welenc ◽  
Andrzej Koncicki
Keyword(s):  
Respiratory Tract ◽  
Underlying Mechanism ◽  
Viral Disease ◽  
Immune Memory ◽  
System Response ◽  
Upper Respiratory Tract ◽  
Resistance Factors ◽  
Infectious Bronchitis ◽  
Immune Mechanisms ◽  
Worldwide Distribution

Infectious bronchitis (IB) is a highly contagious, viral disease of chickens that causes damage to the respiratory tract, kidneys, gastrointestinal and reproductive systems, as well as muscles. Despite the worldwide distribution of vaccines against IB, the outbreaks of this disease are recorded frequently. This review paper describes the mechanisms of the immune system response against both infectious bronchitis virus (IBV) and vaccine IBV, with special attention to the local upper respiratory tract immune mechanisms stimulated in the course of IB. CD8+ T cells as well as IgA+ and IgY+ B memory cells seem to play the most important role in protection against re-infection with IBV. The present paper describes in detail the stimulation of non-specific innate resistance factors and the underlying mechanism of IBV innate immunity breach, as well as the stimulation and acquisition of specificity and immune memory against IBV by immunocompetent cells after both infection and vaccination.

scholarly journals Epidemiological situation of human leptospirosis in Brazil and challenges in its diagnosis with a focus on molecular approaches

Biotemas ◽  
2021 ◽  
Vol 34 (4) ◽  
pp. 1-13
Author(s):  
Andressa Penedo de Paiva Estrella ◽  
Amanda Silva Hecktheuer ◽  
Fabienne Antunes Ferreira ◽  
Ricardo Ruiz Mazzon
Keyword(s):  
Diagnostic Test ◽  
Agglutination Test ◽  
Diagnostic Methods ◽  
Polymorphism Analysis ◽  
General View ◽  
Routine Test ◽  
Human Leptospirosis ◽  
Molecular Approaches ◽  
Worldwide Distribution ◽  
Causative Agents

Leptospira interrogans is one of the causative agents of human leptospirosis, a zoonotic disease with worldwide distribution. Nowadays, this zoonosis is considered one of the biggest in terms of morbidity and mortality (even considering Dengue, the major arbovirosis affecting humans), having in Brazil 3,800 human cases per year. Currently, difficulties imposed by the absence of a rapid, sensitive diagnostic test that can be used as a routine test for the detection of leptospirosis lead to misdiagnosis and underreported cases. The gold standard diagnostic test for leptospirosis is the microscopic agglutination test (MAT), which presents difficulties in execution and interpretation. Therefore, this review proposes a general view of the epidemiologic situation of the disease in Brazil, in addition to the current contributions in the literature for the development of new diagnostic methods. Amongst them, the gene sequences polymorphism analysis, which presents potential for phylogenetic and populational analysis and genotyping of Leptospira spp.

scholarly journals Enhancing Colorimetric LAMP Amplification Speed and Sensitivity with Guanidine Chloride

2020 ◽  
Author(s):  
Yinhua Zhang ◽  
Guoping Ren ◽  
Jackson Buss ◽  
Andrew J. Barry ◽  
Gregory C. Patton ◽  
...  
Keyword(s):  
Guanidine Hydrochloride ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Simple Method ◽  
Diagnostic Assays ◽  
Dna And Rna ◽  
Target Dna ◽  
Versatile Technique ◽  
Guanidine Chloride ◽  
Primer Sets

AbstractLoop-mediated isothermal amplification (LAMP) is a versatile technique for detection of target DNA and RNA, enabling rapid molecular diagnostic assays with minimal equipment. The global SARS-CoV-2 pandemic has presented an urgent need for new and better diagnostic methods, with colorimetric LAMP utilized in numerous studies for SARS-CoV-2 detection. However, the sensitivity of colorimetric LAMP in early reports has been below that of the standard RT-qPCR tests, and we sought to improve performance. Here we report the use of guanidine hydrochloride and combined primer sets to increase speed and sensitivity in colorimetric LAMP, bringing this simple method up to the standards of sophisticated technique and enabling accurate and high-throughput diagnostics.

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