scholarly journals Design, manufacture and test of a dental camera using fluorescence technique

2017 ◽  
Vol 20 (K2) ◽  
pp. 84-90
Author(s):  
Mien Thi Hai Pham ◽  
Dat Tien Nguyen ◽  
Vy Ngoc Khanh Duong ◽  
Vy Thi Hoang Huynh
Keyword(s):  
Dental Caries ◽  
Rapid Test ◽  
Test Time ◽  
Treatment And Prevention ◽  
Oral Health Problem ◽  
Carious Lesions ◽  
Metabolic Products ◽  
Fluorescence Camera ◽  
Industrialised Countries ◽  
Early Caries

Dental caries is a major oral health problem in most industrialised countries and is still a major cause of tooth loss. The early diagnostics of caries is of great importance for children and adults to facilitate the treatment and prevention of the diseases. In this study, a fluorescence camera was designed and manufactured for this purpose. This device includes a 380-nm LED, which stimulates porphyrins - metabolic products of the life cycle of caries-inducing bacteria to emit fluorescence, and a compact camera recording fluorescence images in real time. The device is connected to computer via usb cable. An archiving software helps save shots as image or video. The weight and size of this device are suitable for the visual inspection in oral cavity and can be used in daily dental practice. The test results showed that this fluorescence camera can detect some types of carious lesions including dental plaque, dental caries, hidden caries and early caries. Besides, this tool has a number of advantages such as noninvasiveness, safety (non-ionizing radiation), mobility, rapid test time, and economical.

scholarly journals sCD14 Level in Saliva of Children and Adolescents with and without Dental Caries, a Hurdle Model

Children ◽  
2021 ◽  
Vol 8 (8) ◽  
pp. 679
Author(s):  
Gaia Pellegrini ◽  
Marcello Maddalone ◽  
Matteo Malvezzi ◽  
Marilisa Toma ◽  
Massimo Del Fabbro ◽  
...  
Keyword(s):  
Dental Caries ◽  
Innate Immune ◽  
Hurdle Model ◽  
Soluble Cd14 ◽  
Clear Cut ◽  
Carious Lesions ◽  
Decayed Tooth ◽  
Group A ◽  
The Relationship ◽  
Control Study

Objective: Soluble CD14 (sCD14) plays an important role in the innate immune response of the oral cavity. The investigation of this biomarker for detection of carious lesions is an even more actual procedure due to its non-invasiveness and the ease of withdrawal. The purpose of the present observational case-control study was to evaluate whether the quantification of sCD14 in children and adolescent’s saliva can discriminate healthy subjects from those suffering from tooth decay. Materials and Methods: 164 subjects (6 to 17 years) were selected and divided into 2 groups: those with at least 1 decayed tooth were assigned to group Decayed (n = 82) and those free from dental caries to group Healthy (n = 82). The amount of salivary soluble CD14 was quantified. Results: Mean salivary soluble CD14 was 28.3 ± 10.8 μg/mL in the Healthy group and 22 ± 9.6 μg/mL in the Decayed group. A hurdle model was applied to the data to estimate both the probability of having carious lesions and their number in relation to sCD14 levels. sCD14 was strongly associated (p < 0.01) with an inverse relation to both the probability of having caries and their number (falling rate of 5% per unit CD14 μg/mL). Conclusions: This data confirms the relationship between sCD14 and the presence of dental caries. However, there is no clear cut off level between healthy and unhealthy subjects, so it is currently not possible to use sCD14 as a biomarker to determine the risk of decays.

scholarly journals Case Report: a 5-Year Follow-up of Proximal Caries Lesion Diagnosis

2018 ◽  
Vol 20 (3) ◽  
pp. 158
Author(s):  
Renata Espíndola Silveira ◽  
Isabela Pereira Furtado ◽  
Marco Aurélio Carvalho ◽  
Lawrence Gonzaga Lopes ◽  
João Batista Souza ◽  
...  
Keyword(s):  
Dental Caries ◽  
Clinical Examination ◽  
Oral Hygiene ◽  
Dietary Habits ◽  
Treatment Plan ◽  
Preventive Treatment ◽  
Diagnostic Methods ◽  
Carious Lesions ◽  
Oral Hygiene Instructions

Abstract This clinical case was aimed at reporting and discussing diagnostic methods for early detection and preventive treatment of proximal carious lesions. The patient presented to the dental clinic seeking treatment of a problem she reported as being “dental caries”. The patient’s orthodontist diagnosed dental caries, by means of radiographic exam. There was no evidence of any proximal carious lesion in the intraoral examination. The bitewing radiographs presented a radiolucent area limited to enamel in the mesial of maxillary left-second-bicuspid. For the purpose of confirming the intraoral diagnosis, an immediate tooth separation was performed. The immediate separation was unable to provide sufficient access; thus, the slow separation approach was performed. After 24-hours, tooth separation of 1mm was obtained, providing enough access for clinical examination. The proximal areas were evaluated by transillumination and impression taking. During the visual inspection, an area of enamel staining was observed, with no roughness due to tactile inspection and the evaluation by transillumination and impression showed no cavitation. The following preventive treatment plan was endorsed: oral hygiene instructions with emphasis to the importance of flossing the proximal areas, and healthy dietary habits. After a 5-years period of follow-up, the enhanced oral hygiene and heathy dietary habits were observed. A clinical examination with immediate tooth separation and bitewing radiographs was performed and showed no cavitation. Thecombination of methods for detecting carious lesions in proximal surfaces was effective in obtaining a diagnosis of cariesin the permanent dentition. The absence of cavitated lesions must be indicative of preventive treatment.Keywords: Dental Caries. Preventive Dentistry. Diagnosis, Oral.ResumoEste caso clínico objetivou relatar e discutir os métodos diagnósticos para detecção e tratamento preventivo de lesões cariosas proximais. A paciente procurou atendimento restaurador com queixa de cárie diagnosticada por exame radiográfico realizado após término de seu tratamento ortodôntico. Ao exame clínico não havia suspeita de lesão cariosa. A avaliação radiográfica revelou, na face mesialdo dente 25, presença de área radiolúcida limitada à metade externa do esmalte. Para confirmação do diagnóstico realizou-se a separação interdentária imediata, porém como a mesma não permitiu adequada visualização foi realizada a separação mediata e após 24 horas obteve-se adequada separação (1 mm) para o exame clínico. Em seguida, a superfície proximal foi avaliada por transiluminação e moldagem. Durante a inspeção visual foi constatada área de manchamento do esmalte, com ausência de rugosidade pela inspeção tátil e a avaliação tanto por transiluminação da superfície proximal, quanto por moldagem revelou ausência de cavitação. Devido o diagnóstico obtido o seguinte tratamento preventivo foi indicado: reforço de higiene oral com ênfase na importância do uso do fio dental e hábitos dietéticos saudáveis. Após 5 anos de acompanhamento melhoria da higiene oral e hábitos dietéticos foram constatados. O exame clínico com separação interdentária imediata e exame radiográfico revelaram ausência de cavitação. Conclui-se que a combinação de métodos de detecção de lesões cariosas proximais foi efetivo no diagnóstico da cárie dentária na dentição permanente. O resultado deste relato de caso mostrou que a ausência de lesões cariosas cavitadas deve ser um indicativo de tratamento preventivo.Palavras-chave: Cárie Dentária. Odontologia Preventiva. Diagnóstico Bucal.

scholarly journals Effect of Prebiotics-Enhanced Probiotics on the Growth ofStreptococcus mutans

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Santichai Nunpan ◽  
Chatrudee Suwannachart ◽  
Kornchanok Wayakanon
Keyword(s):  
Growth Rate ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Lactobacillus Acidophilus ◽  
Digestive System ◽  
Acidic Environment ◽  
Beneficial Microorganisms ◽  
Carious Lesions ◽  
Significant Difference ◽  
Stimulation Of

Streptococcus mutanspredominantly creates an acidic environment in an oral cavity. This results in dental demineralization and carious lesions. The probiotics are beneficial microorganisms that modulate the bacterial balance in the digestive system. Prebiotics are defined as nondigestible oligosaccharides that are utilized for the selective stimulation of the beneficial microorganisms. The objective of this study was to evaluate the efficacy of the prebiotics, galactooligosaccharides (GOS) and fructooligosaccharides (FOS), for enhancing the probioticLactobacillus acidophilusATCC 4356, for inhibitingStreptococcus mutans(A32-2) for the prevention of dental caries. The growth rate of theS. mutanssignificantly decreased when cocultured withL. acidophilusin the GOS-supplemented medium at 3%, 4%, and 5%. In the FOS-supplemented medium, the growth rate ofS. mutanssignificantly decreased in all concentrations when cocultured withL. acidophilus. There was no significant difference in the growth rate ofL. acidophilusin all concentrations of either GOS or FOS. It can be concluded that the growth rate ofS. mutanswas significantly retarded when cocultured withL. acidophilusand the proper concentration of prebiotics. These prebiotics have potential for a clinical application to activate the function of the naturally intraoralL. acidophilusto inhibitS. mutans.

Immunization with Dextransucrases, Levansucrases, and Glycosidic Hydrolases from Oral Streptococci. II. Immunization with Glucosyltransferases, Fructosyltransferases, and Glycosidic Hydrolases from Oral Streptococci in Monkeys

1977 ◽  
Vol 56 (12) ◽  
pp. 1586-1598 ◽  
Author(s):  
Arthur N. Bahn ◽  
Irving L. Shklair ◽  
James A. Hayashi
Keyword(s):  
Dental Caries ◽  
Macaca Mulatta ◽  
Rhesus Monkeys ◽  
Control Group ◽  
Oral Streptococci ◽  
Primate Model ◽  
Pathological Effect ◽  
Carious Lesions ◽  
Gross Lesions

The feasibility of immunizing monkeys with enzymes from oral streptococci in an attempt to reduce dental caries was investigated. Forty rhesus monkeys, Macaca mulatta, were used. Cariogenic streptococci, S mutans, were implanted into all the monkeys' mouths. There was no pathological effect resulting from immunization. Of the 40 animals, 30 retained the implanted flora throughout the experiment; the remaining 10 were reimplanted until the streptococci remained. In six months, gross carious lesions were evident with plaque. Inhibitors present in the monkey sera after immunization inhibited glucosyltransferase, fructosyltransferase, and neuraminidase activzties. It was presumed the inhibitors were antibodies. There was a reduction of 68.6% in the total carious lesions in the animals immunized intraorally with glucosyltransferase, 62.4% reduction in those injected with fructosyltransferase, and 57.4% reduction in total lesions in those immunized with glycosidic hydro lases after 19 months, as compared to the control group. There were no gross lesions apparent in the group immunized with glycosidic hydrolases. It appears that immunization with enzymes significantly reduces caries and is feasible in a primate model.

scholarly journals Antimicrobial Efficacy of Toothpastes Containing Fluoride against Clinically Isolated Streptococci mutans

2020 ◽  
pp. 19-24
Author(s):  
Eze E. Ajaegbu ◽  
Calister U. Ezeh ◽  
Adaobi J. Dieke ◽  
Adaora L. Onuora ◽  
Jane I. Ugochukwu
Keyword(s):  
Antimicrobial Activity ◽  
Dental Caries ◽  
Antimicrobial Properties ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Oral Diseases ◽  
Laboratory Methods ◽  
Chemical Substances ◽  
Carious Lesions ◽  
Agar Well Diffusion Method

Dental caries is one of the prevalent oral diseases which affect all ages. Many chemical substances with antimicrobial properties, are effective against microorganisms causing dental caries. The objective of this study was to check the antimicrobial efficacy of some toothpastes  found in Enugu metropolis, Nigeria more specifically against clinically isolated Streptococci mutans. The antimicrobial efficacy of different brands of toothpastes marketed in Enugu metropolis  was assayed against Streptococci mutans using the agar well diffusion method. The clinical Streptococci mutans used for this study were taken from carious lesions of patients that  attended the clinic at Federal College of Dental Therapy and Technology, Trans Ekulu, Enugu, Nigeria and were isolated and cultivated using traditional laboratory methods. The present  study has shown that toothpaste which contains mint extract as herbal constituent and sodium fluoride had the highest antimicrobial activity, followed by toothpastes containing only sodium monofluorophosphate. Herbal toothpaste with mint can be a safe and effective alternative in the prevention of dental caries.

scholarly journals Inhibitory Effect of Lactobacillus plantarum CCFM8724 towards Streptococcus mutans- and Candida albicans-Induced Caries in Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Qiuxiang Zhang ◽  
Sujia Qin ◽  
Xianyin Xu ◽  
Jianxin Zhao ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Dental Caries ◽  
Oral Cavity ◽  
Lactobacillus Plantarum ◽  
Streptococcus Mutans ◽  
Microbial Colonization ◽  
Control Group ◽  
Scoring Method ◽  
Treatment And Prevention

Streptococcus mutans is a recognized cariogenic bacterium and a major producer of biofilm matrix. The presence of Candida albicans in dental plaque with S. mutans enhances the virulence leading to the onset of rampant caries which is similar to early childhood caries (ECC). The purpose of this study was to explore the effect of Lactobacillus plantarum CCFM8724 (CCFM8724) on the treatment and prevention of dental caries induced by S. mutans and C. albicans in vivo. Rats were divided into 6 groups: the control group and model group, 2 treatment groups, and 2 prevention groups (0.02% chlorhexidine or CCFM8724). The fluctuation of microbial colonization and the change of bacteria flora in rat oral cavity after sowing of L. plantarum CCFM8724 were investigated by colony-forming units (CFU) and microflora analysis. The caries of rats were assessed by microcomputed tomography (micro-CT) and Keyes scoring method. The results showed that L. plantarum CCFM8724 in both the treatment and prevention groups could significantly decrease the population of S. mutans and C. albicans in the rats’ oral cavity ( p < 0.001 ), the mineral loss of enamel ( p < 0.05 ), and the scores of caries ( p < 0.05 ). Besides, L. plantarum CCFM8724 exhibited better effects than chlorhexidine. Hence, L. plantarum CCFM8724 was proved to be a potential oral probiotic on caries treatment and prevention in vivo and it may have the prospect of application in dental caries (especially ECC) prevention products.

scholarly journals THE CHEMICAL COMPOSITION OF THE SURFACE LAYER OF ENAMEL DEPENDING ON ITS FUNCTIONAL STATE

2018 ◽  
pp. 66-71
Author(s):  
N. N. Pustovoitova ◽  
V. V. Kitel
Keyword(s):  
Surface Layer ◽  
Czech Republic ◽  
Great Britain ◽  
Chemical Composition ◽  
High Resolution ◽  
Dental Caries ◽  
Light Elements ◽  
Spectral Analyzer ◽  
Carious Lesions ◽  
Calcium Phosphorus

Objective: to study the chemical composition of the surface layer of enamel in dental caries depending on the activity of carious lesions. Material and methods. The chemical composition of the surface layer of enamel in teeth samples with arrested (n = 15) and active (n = 15) caries was studied using the high resolution SEM «Mira» by the firm «Tescan» (Czech Republic) with the micro spectral analyzer «INCA Energy 350» of the firm «Oxford Instruments Analytical» (Great Britain). Results. We have revealed reliable increases in carbon, oxygen and other light elements in the surface layer of the enamel at the area of active carious lesions in comparison with intact enamel and arrested carious lesions; significant decreases in the content of calcium, phosphorus, ratio of Ca / P coefficient for the surface layer of the enamel of active carious lesions in comparison with intact enamel and arrested carious lesions. We have revealed no reliable differences in the content of C, O, Ca, P, CI in the surface layer of intact enamel and that at the area of arrested no cavity caries.

scholarly journals ASSESSMENT OF DENTAL CARIOUS LESIONS BY ICDAS II

2021 ◽  
Vol 21 (1) ◽  
pp. 76-80
Author(s):  
V. Melnik ◽  
L. Gorzov ◽  
S. Melnik ◽  
Ya. Duganchik
Keyword(s):  
Dental Caries ◽  
Age Groups ◽  
Dental Enamel ◽  
Statistical Processing ◽  
Caries Detection ◽  
Average Intensity ◽  
Initial Caries ◽  
Carious Lesions ◽  
Icdas Ii ◽  
Caries Lesions

Introduction. The largest amount of information about the dental caries is provided by the International Caries Detection and Assessment II system (ICDAS II), which is known as evidence-based approach to clinical visual detection of dental caries and enables to detect the stage and depth of carious lesions from the slightest changes in dental enamel to visible cavities affecting dentin. This system has been developed by the group of leading experts in the field of cariology. The results obtained by determining the ICDAS II index contribute to making right clinical decision in the choice of prevention and treatment methods, as well as to predicting the carious progression. The aim of the study is to assess the prevalence and intensity of initial dental caries in people using the ICDAS II index. Materials and methods. A total of 32 patients aged 12 to 25 years were examined. During the dental examination, we assessed the prevalence of dental caries using the ICDAS II. Clinical findings were recorded in oral follow-up charts proposed by the ICDAS Foundation for Epidemiological Studies, which allow us to record dental hard tissue status using six codes: three for assessing carious changes in enamel and three for assessing carious changes in dentine in a sequence of growing severity. Statistical processing of the findings was performed using Student's t-test. Results. Carious lesions were detected in all study participants, their total number was 285, of which 140 (49.1%) had the code 1 and 145 (50.8%) had the code 2 according to the ICDAS II. On average, each of the participants had 9.28 ± 0.67 foci of enamel demineralization. The average number of lesions with codes 1 and 2 by the ICDAS II was respectively 4.54 ± 0.51 and 4.74 ± 0.38 (p> 0.05). The average intensity of dental caries increased with age from 8.29 ± 0.83 in 12-15 year old individuals to 9.39 ± 1.20 in 18-25 year old individuals (p> 0.05), mainly by the growth of the average number of the mean number of caries lesions with the code 1 according to the ICDAS II. Mostly carious lesions are found on the masticatory surfaces, their total number made up 159 (55.7%). 101 (35.4%) caries lesions were found on the vestibular and oral parts of the tooth surfaces, and 25 (8.9%) average lesions were detected on the proximal surfaces. The average intensity of caries detected on the chewing surfaces of the teeth was 5.15 ± 0.49 and was significantly higher than on the vestibular and oral (3.33 ± 0.57, p <0.05) and proximal (0.79 ± 0, 20, p <0.001) surfaces. Conclusion. The obtained results showed a high intensity of carious lesions, with their predominance in the active stages in the two age groups under the study. This proves the appropriateness of using diagnostic ICDAS criteria for early detection of initial caries and its proper treatment.

scholarly journals Oral Health Problem Among Pregnant Mothers: Age And Attitude Do Matter

2017 ◽  
Vol 16 (2) ◽  
Author(s):  
Karimah Hanim binti Abd Aziz ◽  
Nurjasmine Aida bt Jamani ◽  
Zurainie bt Abllah
Keyword(s):  
Oral Health ◽  
Dental Caries ◽  
Low Socioeconomic Status ◽  
Health Problem ◽  
Multinomial Logistic Regression ◽  
Well Being ◽  
Oral Health Problem ◽  
Periodontal Index ◽  
Pregnant Mothers ◽  
Good Attitude

Introduction: Oral health care during pregnancy is greatly important as it is crucial to the health and well-being to both pregnant mother and baby. Studies showed that mothers with oral health problem have greater risk of prematurity and low birth weight babies. This study aimed to measure the associated factors of oral health problem among pregnant mothers. Materials and Methods: This cross sectional study was conducted among purposely selected 296 pregnant mothers aged 18- 45 years from the biggest district in Pahang State, Malaysia. This 18 months study used validated self-administered questionnaire which was asking on socio-demographic characteristics, and knowledge, attitude and practice on oral health. Oral health status was assessed by dentists using Community Periodontal Index of Treatment Need (DFMT index). Descriptive analysis used to describe respondents background and multinomial logistic regression used to examine the associations. Results: Majority of the respondents (89.9%) were among young mothers (35 years), Malay (91.9%), mean gestation of two, and from low socioeconomic status (59.5%). Of the 296 respondents, 111 (48.1%) had low experience with dental caries, 57 (24.7%) had moderate experience, and 63 (24.3%) had high experience with dental caries. Older age had higher risk to experience dental caries and those who had good attitude were protected, p=0.018 and p=0.023 respectively. Conclusion(s): Age and attitude were significant factors associated to oral health problem among pregnant mothers. Thus, health promotion and good attitude should be instilled from younger age.

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