scholarly journals Optimizing beef cattle performance using rumen-protected lysine supplementation in diets balanced for predicted amino acid and effective energy requirement

2017 ◽  
Author(s):  
◽  
Jacquelyn Prestegaard
Keyword(s):  
Beef Cattle ◽  
Continuous Culture ◽  
Soybean Meal ◽  
Dry Matter ◽  
Energy Requirement ◽  
Latin Square ◽  
Negative Control ◽  
Effective Energy ◽  
Dietary Treatments

The objectives of these experiments were to determine the bypass value of rumen-protected lysine and performance responses of beef cattle fed encapsulated lysine. During experiment 1 singe-flow continuous culture fermenters were fed a Lys-deficient control (CON), a Lys-sufficient diet supplemented with rumen-protected soybean meal (RPSBM; AminoPlus, Ag Processing Inc., Omaha, NE), or a Lys-sufficient diet containing a commercially available rumen-protected Lys product (RPLYS; USA Lysine, Kemin Industries Inc., Des Moines, IA). Results from continuous culture showed greater degradation of RPLYS than RPSBM and CON. During Experiment 2, an in vitro dry matter digestibility study showed DM degradation was 23 percent greater for pure USA Lysine than another encapsulated lysine product (AjiPro 2G, Ajinomoto Heartland, Inc., Omaha, NE). The next objective was to test if AjiPro would increase plasma Lys levels in vivo (and therefore indicate its successful ruminal bypass and small intestinal absorption). Experiment 3 involved a 3 x 3 Latin Square study conducted on cannulated crossbred steers fed a combination of rumen-protected soybean meal and two increased levels of the rumen-protected product, where it was fed to meet 100 percent (AJ100) and 150 percent (AJ150) absorbable AA to effective energy (EE) ratio. Plasma Lys levels were greater when steers were fed diets containing AjiPro in comparison to when they were fed a negative control (NEGCON) which did not contain the product and was deficient in absorbable Lys. From both the in vitro dry matter degradation experiment and the 3 x 3 Latin Square study, we determined AjiPro to be an effective source of bypass Lys, and consequently used it to conduct a growing through finishing study. During Experiment 4, we evaluated steer performance when fed diets balanced for predicted Lys requirement to EE ratio through its supplementation in several dietary treatments. Control treatments included a negative control (NEGCON) that was deficient in absorbable Lys and contained no rumen-protected products; and a positive control (POSCON) where rumen-protected soybean meal was used to balance absorbable AA to EE ratio. Three additional dietary treatments included similar amounts of rumen-protected soybean meal and incremental amounts of AjiPro formulated to provide 50 percent (AJ50), 100 percent (AJ100), or 150 percent (AJ150) of the absorbable Lys provided by POSCON. Starting on d 151 of the growth study, steers were weighed on 2 consecutive days every 14 d and assigned a final BW when no longer profitable (defined as when cost of gain exceeded value of gain). Steers remained profitable for greater days for NEGCON, POSCON and AJ100 than AJ150. Steers consuming POSCON had lesser ADG (kg/d) than all other treatments during the early finishing phase (d 75 to 112). However, steer ADG (kg/d) during late finishing (d 112 to 179) was greater for steers fed diets optimized for Lys requirement (POSCON and AJ100) than all other treatments. Between d 112 to 179, POSCON had greater G:F than all other treatments but did not differ from AJ100. When encapsulated Lys was under or over-supplemented, finishing steers became less profitable sooner. Feed efficiency increased with use of rumen-protected products during late finishing (d 112 to 179) in diets formulated to meet Lys requirement, but this did not impact overall steer performance from growing through finishing. A more accurate understanding of steer AA requirement and subsequent AA metabolism will allow more precise and effective use of rumenprotected products.

scholarly journals Sunflower cake in diets for beef cattle: digestibility, kinetics and in vitro ruminal fermentation parameters

2018 ◽  
Vol 41 (1) ◽  
pp. 39492 ◽  
Author(s):  
Rafael Henrique de Tonissi e Buschinelli de Goes ◽  
Luiz Henrique Xavier da Silva ◽  
Tatiana García Díaz ◽  
Antonio Ferriani Branco ◽  
Ana Lúcia Teodoro ◽  
...  
Keyword(s):  
Organic Matter ◽  
Beef Cattle ◽  
Soybean Meal ◽  
Crude Protein ◽  
Dry Matter ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Fermentation Products ◽  
Sunflower Cake

 The objective of this study was to evaluate the effect of the inclusion of sunflower cake replacing soybean meal in beef cattle diets on the in vitro digestibility of dry matter (IVDDM), organic matter (IVDOM), crude protein (IVDCP) and the ruminal fermentation kinetics and parameters. The experiment was analyzed according to a completely randomized design. The treatments consisted of four levels of sunflower cake, 0, 200, 400, 600 g kg-1, replacing soybean meal in the concentrate of beef cattle diets. The coefficients of IVDDM, IVDOM and IVDCP presented a quadratic effect with the addition of sunflower cake. The soluble fraction (fraction B) degradation rate and total gas production decreased linearly with the inclusion of sunflower cake. Values of pH in ruminal fluid were higher for levels 0, 200 and 600 g kg-1 sunflower cake. Sunflower cake can replace soybean meal by up to 280 g kg-1 in the concentrate of beef cattle diets, improving the in vitro digestibility of dry matter organic, matter and crude protein. Levels above 400 g kg-1 reduce ruminal digestion rate, digestibility and release of final fermentation products. 

scholarly journals Slow-Release Urea as a Sustainable Alternative to Soybean Meal in Ruminant Nutrition

2021 ◽  
Vol 13 (5) ◽  
pp. 2464
Author(s):  
Saheed A. Salami ◽  
Maria Devant ◽  
Juha Apajalahti ◽  
Vaughn Holder ◽  
Sini Salomaa ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Soybean Meal ◽  
Dry Matter ◽  
Vegetable Protein ◽  
Slow Release ◽  
Protein Sources ◽  
Slow Release Urea ◽  
In Vitro Rumen Fermentation

Three experiments were conducted to evaluate the feasibility of using a commercial slow-release urea product (SRU; Optigen®, Alltech Inc., Nicholasville, KY, USA) as a partial replacement for vegetable protein sources in cattle diets. The first experiment was an in vitro rumen fermentation that evaluated the effect of replacing soybean meal (SBM) nitrogen with nitrogen from either SRU or free urea in diets varying in forage:concentrate ratios. The second experiment examined the effect of replacing SBM with SRU on in situ dry matter and nitrogen degradability in the rumen. In the third experiment, a feeding trial was conducted to evaluate the effect of replacing SBM (0% as-fed SRU) with 1% or 3% as-fed SRU on feed carbon footprint (CFP; total greenhouse gas emissions associated with the life cycle of feed raw materials) and the toxicity potential of SRU in growing beef cattle. Results showed that replacing SBM with SRU up to 1.3% did not negatively affect in vitro rumen fermentation parameters. Supplementing SRU favourably decreased ruminal accumulation of ammonia and lactic acid when compared to free urea. There was no significant effect on effective rumen degradability of dry matter and nitrogen when one-third of SBM was replaced by SRU in the in situ study. Compared with the 0% SRU diet, feed CFP decreased by 18% and 54% in 1% SRU and 3% SRU diets, respectively. Additionally, feeding up to 3% SRU diet to beef cattle did not affect health and intake, and blood hematological and biochemical indices were within the physiological range for healthy bulls, suggesting no indication of ammonia toxicity. Overall, these results indicate that SRU can be used as a sustainable alternative to partially replace vegetable protein sources in ruminant diets without compromising rumen function and health of ruminants.

Cassava root fermented with yeast improved feed digestibility in Brahman beef cattle

2017 ◽  
Vol 57 (8) ◽  
pp. 1613 ◽  
Author(s):  
C. Promkot ◽  
P. Nitipot ◽  
N. Piamphon ◽  
N. Abdullah ◽  
A. Promkot
Keyword(s):  
Beef Cattle ◽  
Dry Matter ◽  
Control Diet ◽  
Latin Square ◽  
Nutrient Digestibility ◽  
Dry Matter Intake ◽  
Neutral Detergent Fibre ◽  
Feed Digestibility ◽  
Cassava Root ◽  
Dietary Treatments

Saccharomyces cerevisiae has been used to enhance the protein level of low-protein feedstuffs such as cassava root. In the present study, fresh cassava root was grated and subjected to solid-state fermentation with S. cerevisiae to enhance the protein content. The fermentation process lasted for 21 days, followed by fermentation with yeast for 5 days. The fermented product was called yeast-fermented cassava root (YEFECAR). The YEFECAR was then evaluated as a feed ingredient at 10%, 20% and 30% dry matter in concentrate diet for dry matter intake and nutrient digestibility in non-pregnant female Brahman beef cattle (bodyweight 256 ± 11.5 kg). Four cattle were randomly given four treatments in 4 × 4 Latin square design for 21-day period, including a control diet (without fermented cassava root). Cattle were fed rice straw as roughage. The results showed that while dry matter intake was not significantly different among dietary treatments, average dairy gain tended to increase when fermented cassava root was fed at 20% in the concentrate diet. There were no significant differences in nutrient digestibility among the dietary treatments. However, neutral detergent fibre and acid detergent fibre digestibility showed an increasing trend when fermented cassava root was fed to the cattle. Likewise, fermented cassava root showed no effect on rumen microbial population, although rumen bacterial population and microbial protein tended to increase with YEFECAR addition. It was concluded that yeast-fermented cassava root at 20% in concentrate feed tended to enhance rumen bacteria population and neutral detergent fibre digestibility in cattle.

scholarly journals PSI-24 Relationship between passage rate and in vitro methane production from rumen and fecal contents

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 240-241
Author(s):  
Corina Nunez ◽  
Sara M Tondini ◽  
Thomas G Jennings ◽  
Justin W Rickard ◽  
Angela R Green-Miller ◽  
...  
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Latin Square ◽  
Negative Control ◽  
Gas Production ◽  
Buffer Solution ◽  
Passage Rate ◽  
Methane Production Rate ◽  
Ad Libitum

Abstract The objective was to evaluate treatments designed to increase passage rate on the in vitro methanogenic potential of rumen and fecal contents. Eight ruminal-cannulated steers were used in a replicated 4×4 Latin square design with data collected from the latter three 24 d periods. All steers were fed an ad libitum diet of grass hay (10.4% CP) with the following treatments designed to increase passage rate: negative control (CON), pelleted (PEL), 7% Ca(OH)2 treated (CAOH), and four 6 kg weights added to the rumen (WTS). Molasses (3% of diet DM) was added to all treatments to improve diet palatability. On d 22 of each period, fecal and rumen samples for in vitro procedure were collected 0 and 2 hr after feeding, respectively. Rumen (10 g) and fecal (20 g) samples were added to 100 ml Wheaton vials with buffer solution at 1:5 and 1:2 ratio, respectively. Vials were incubated at 39°C, and samples were collected at 6 and 24 hr to determine gas production. Methane was analyzed using gas chromatography. Data were analyzed using the MIXED procedure of SAS. In vitro methane production rate from rumen samples tended (P = 0.09) to be affected by treatment with the greatest rate observed for CON (11.7 mM per g DM per h) and the least for PEL (6.3 mM per g DM per h). Rate of fecal methane production in vitro was not affected (P = 0.48) by treatment with means ranging from 0.79 to 2.3 mM per g DM per h. Predicted daily methane production from fecal dry matter excretion was also not affected (P = 0.98) by treatment. Treatments designed to increase passage rate may affect the methanogenic potential of archaea in ruminal contents but not in feces.

scholarly journals Effects of slow-release urea on in vitro rumen fermentation parameters, growth performance, nutrient digestibility and serum metabolites of beef cattle

2020 ◽  
Vol 41 (4) ◽  
pp. 1399
Author(s):  
Huan Liang ◽  
Erlong Zhao ◽  
Chunyan Feng ◽  
Jianfei Wang ◽  
Lanjiao Xu ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Soybean Meal ◽  
Dry Matter ◽  
Slow Release ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Serum Metabolites ◽  
Fermentation Parameters ◽  
Slow Release Urea

Two experiments were conducted to investigate the effects of slow-release urea (SRU) on in vitro rumen fermentation parameters, growth performances, nutrient digestibility, and serum metabolites of beef cattle. The single factor design was applied in both experiments. Three diets with different nitrogen sources including soybean meal (Control group), slow-release urea (SRU group), and common urea (Urea group) was designed (concentrate to forage ratio was 4:6). The diets were formulated to be isoenergetic and isonitrogenous, 75% of the soybean meal in the control diet was replaced by 1.41% SRU and 1.15% urea in SRU group and Urea group, respectively. In experiment 1, five healthy Jinjiang cattle (average body weight (BW) was 380 ± 17.1 kg) with permanent rumen fistulas were used in in vitro ruminal fermentation experiment. The results showed that supplementing SRU increased the dry matter degradation rate (DMD), digestible organic matter (DOM) and propionic acid concentration in cultivated fluid, and SRU supplementation decreased pH, NH3-N, total volatile fatty acid (TVFA), acetic acid, butyric acid concentration and microbial growth efficiency (MOEFF) in cultivated fluid. In experiment 2, eighteen Simmental crossbred cattle BW= 315 ± 5.2 kg) were stratified by BW and then assigned to the three groups to have equal BW among groups. The results showed that supplementing SRU reduced the average dry matter intake (ADMI), apparent digestibility of ether extract (EE), the activity of glutathione peroxidase (GSH-Px), the levels of IgG and IgA, and the production of thiiodothronine (T3) in serum, SRU supplementation increased the apparent digestibility of dry matter and organic matter (OM) and alanine aminotransferase (ALT) concentration in serum. These results indicated that some soybean meal could be replaced by SRU and urea in the production of beef cattle. In addition, compared with urea, SRU had a good sustained-release effect. The replacement of some soybean meal by SRU in the diet had no adverse impact on rumen fermentation, growth performance, and serum metabolites of beef cattle.

scholarly journals PSIV-1 Apparent and Standardized Ileal Digestibility of Amino Acids in Dry Extruded-Expelled Soybean Meal Fed to Growing Pigs Without or With Organic Acids and Multi-Carbohydrase

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 275-276
Author(s):  
Jichen Song ◽  
Charles Martin Nyachoti
Keyword(s):  
Soybean Meal ◽  
Mixed Model ◽  
Interactive Effect ◽  
Latin Square ◽  
Growing Pigs ◽  
Final Model ◽  
Ileal Digestibility ◽  
Positive Effects ◽  
Dietary Treatments ◽  
Main Effects

Abstract Dry extruded-expelled soybean meal (DESBM) is an attractive feed ingredient for swine not only as a protein source but also as a good source of energy because of its high fat content. Apparent (AID) and standardized (SID) ileal amino acid (AA) digestibility in DESBM fed without or with organic acid (OA) and a multicarbohydrase (MC) supplementation to growing pigs were determined. Ten ileal-cannulated barrows (17.3 ± 0.4 kg BW) were randomly assigned to 1 of 5 dietary treatments in a replicated 5 × 4 incomplete Latin square design with 4 periods, each consisting of 5 d of adaptation and 2 d of ileal digesta collection. Four cornstarch-based diets with 44.4% DESBM as the only source of AA, without or with OA and MC supplementation, and a 5% casein-cornstarch diet were used. All diets contained 0.3% titanium dioxide. Data were analyzed using the MIXED model procedures of SAS, with the final model including OA, MC, and the interaction between OA and MC as the main effects. The SID of indispensable AA in DESBM fed to growing pigs were: Arg, 93.2%; His, 85.1%; Ile, 87.1%; Leu, 87.6%; Lys, 88.7%; Met, 87.8%; Phe, 88.4%; Thr, 81.4%; Trp, 73.6%; Val, 84.5%. OA supplementation decreased the AID and SID (P < 0.05) of Arg, Ile, Asp, and Glu, and tended (P < 0.10) to decrease the AID and SID of Phe, Trp, Cys, Gly, and Tyr. MC supplementation did not affect the AID and SID of AA, and no interactive effect on AA digestibility was observed between OA and MC supplementation. In conclusion, dietary supplementation of OA and MC showed no positive effects on improving AA digestibility in DESBM fed to growing pigs.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P < 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

PSVI-13 Amino Acid Supplementation During the Adaptation Period Did Not Affect the Standardized Ileal Digestibility of Amino Acids in Corn and Soybean Meal Fed to Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 200-201
Author(s):  
Hyunjun Choi ◽  
Sun Jong You ◽  
Beob Gyun G Kim
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Soybean Meal ◽  
Latin Square ◽  
Sole Source ◽  
Adaptation Period ◽  
Amino Acid Supplementation ◽  
Ileal Digestibility ◽  
Indispensable Amino Acids ◽  
Dietary Treatments

Abstract The objective was to determine the influence of amino acid (AA) supplementation during the adaptation period on the ileal digestibility of crude protein and AA in corn and soybean meal (SBM). Six barrows with an initial body weight of 30.9 ± 2.6 kg fitted with a T-cannula in the distal ileum were assigned to a 6 × 6 Latin square design with 6 dietary treatments and 6 periods. Two experimental diets contained corn or SBM as the sole source of AA and an N-free diet was additionally prepared. For AA supplementation groups, an AA mixture consisted of Gly, Lys, Met, Thr, Trp, Ile, Val, His, and Phe was added to the corn diet and the N-free diet at the expense of cornstarch, and an AA mixture of Lys, Met, and Thr was added to the SBM diet. All diets contained 0.5% of chromic oxide. The 6 experimental diets were fed to the pigs for 4 and half days, and the 3 diets containing AA mixture were switched to the respective diets without AA mixture during the following 2 and half days. Ileal digesta were collected during the last 2 days. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Arg and Trp in corn (P < 0.05), but did not affect that in SBM. The addition of AA mixture during the adaptation period caused increased apparent ileal digestibility of Pro and Gly regardless of feed ingredient (P < 0.05), but did not affect that of other AA. All AA except Pro in corn and SBM were unaffected by the addition of AA mixture during the adaptation period. In conclusion, the addition of amino acid during the adaptation period does not affect the standardized ileal digestibility of indispensable amino acids in feed ingredients.

scholarly journals Dietary rambutan peel powder as a rumen modifier in beef cattle

2020 ◽  
Vol 33 (5) ◽  
pp. 763-769
Author(s):  
Thiwakorn Ampapon ◽  
Metha Wanapat
Keyword(s):  
Beef Cattle ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Neutral Detergent Fiber ◽  
Napier Grass ◽  
Feed Consumption ◽  
Ruminal Fermentation ◽  
Fruit Peel ◽  
Negative Effect

Objective: The experiment was conducted to study the effect of rambutan (Nephelium lappaceum) fruit peel powder (RP) on feed consumption, digestibility of nutrients, ruminal fermentation dynamics and microbial population in Thai breed cattle.Methods: Four, 2-year old (250±15 kg) beef bull crossbreds (75% Brahman×25% local breed) were allotted to experimental treatments using a 4×4 Latin square design. Four dietary supplementation treatments were imposed; non-supplementation (control, T1); supplementation of RP fed at 2% of dry matter intake (DMI) (low, T2); supplementation of RP fed at 4% of DMI (medium, T3) and supplementation of RP fed at 6% of DMI (high, T4). All cattle were given a concentrate supplement at 1% of body weight while Napier grass was provided as a free choice.Results: The findings revealed that RP supplementation did not negatively affect (p>0.05) DMI of Napier grass, while RP intake and total DMI were the greatest in the RP supplementation at 4% and 6% DMI. Nevertheless, the nutrients (dry matter, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber) digestibilities were not changed in the RP supplementation groups. Rumen fermentation parameters especially those of total volatile fatty acids, acetate and butyrate were not significantly changed. However, the propionate concentration was remarkably increased (p<0.05) in the RP supplementation. Notably, the ratio of acetate to propionate, the number of protozoa, as well as the methane estimation were significantly reduced in the RP supplemented groups (4% and 6% of DMI), while the counts of bacteria was not altered.Conclusion: Supplementation of RP (4% of DMI) improved rumen propionate production, reduced protozoal population and methane estimation (p<0.05) without a negative effect on feed consumption and nutrients total tract digestibilities in beef cattle. Using dietary rambutan fruit peel powder has potential promise as a rumen regulator.

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