scholarly journals MEMS Impedance Biosensor for accurate and rapid detection of E.coli 0157:H7

2016 ◽  
Author(s):  
◽  
Shibajyoti Ghosh Dastider
Keyword(s):  
Electrode Array ◽  
Electrode Pair ◽  
Impedance Change ◽  
Bacterial Cells ◽  
Electrode Arrays ◽  
Measurement Sensitivity ◽  
Functional Region ◽  
Bulk Fluid ◽  
Treatment Techniques ◽  
Previous Design

Two Impedance biosensors based on interdigitated electrode (IDE) arrays were designed, fabricated and tested for detection of low concentration Escherichia coli O157:H7. The first biosensor consists of two set of gold IDE arrays embedded in a SU8-PDMS microchannel. Positive dielectrophoresis (p-DEP) is used to focus and concentrate the E.coli cells into the centre of the microchannel, using the first IDE array. The concentrated cells are then guided towards the sensing region microchannel, which has one-third the width of the initial microchannel. The bulk fluid keeps flowing toward the outer channel towards the waste outlets. The second IDE array located in the sensing region is used for impedimetric detection of the E.coli cells. A combination of standard photolithography, wet etching and plasma treatment techniques were used to fabricate the biosensor. The E.coli cells in the test solution were focused into the centre of the channel when excitation signal of 5 Vp-p at 5.6 MHz was applied across the electrode arrays. Before injecting the E.coli cells, polyclonal anti-E.coli antibodies were non-specifically immobilized on the sensing electrode array. This ensures specific detection of E.coli O157:H7 bacterial cells. As the concentrated E.coli cells (antigen) reach the sensing electrode array, they bind to the immobilized antibody sites. This antigen-antibody binding causes a change in the impedance which is measured using an impedance analyzer. The device performance was tested by measuring the impedance, between 100 Hz-1 MHz frequency, before and after applying p-DEP on the focusing electrode array, and after applying p-DEP on both the focusing and sensing electrodes. The result shows clearly that the use of p-DEP on the focusing IDE array significantly increased the measurement sensitivity with the lower detection limit being 3x10^2 CFU/mL. In addition, the use of p-DEP on both electrode arrays increased the measurement sensitivity by a factor of 2.9 to 4.5 times depending on the concentration. The second biosensor consists of a redesigned focusing region and multielectrode sensing region to improve the efficiency and to be able to detect even lower concentration E.coli O157:H7. Similar to the previous design this biosensor also consists of two functional region: focusing and sensing region. In this design, the focusing region consists a ramp down vertical electrode pair made of electroplated gold along with tilted (45 degree) thin film finger pairs, embedded in a microchannel. This configuration improves the concentration and focusing of the bacteria into the center of the microchannel, and direct them towards the sensing region. The sensing region consists of three IDE arrays, with varying number of electrode fingers (30, 20 and 10 pairs respectively), all embedded inside a narrow microchannel and functionalized using anti-E.coli antibody. As E.coli binds to the antibody, it results in impedance change. The biosensor was fabricated on a glass substrate using SU8 negative photoresist to form the microchannel, gold electroplating to form the vertical focusing electrode pair, thin gold film to form the detection electrode, the finger electrodes, traces and bonding pads, and PDMS to seal the device. This biosensor was able to detect concentrations as low as 39 CFU/mL, which indicates a 7.5 times higher sensitivity, over the previous design.

scholarly journals The Importance of Intraoperative Plain Radiographs during Cochlear Implant Surgery in Patients with Normal Anatomy

2021 ◽  
Vol 11 (9) ◽  
pp. 4144
Author(s):  
Ohad Cohen ◽  
Jean-Yves Sichel ◽  
Chanan Shaul ◽  
Itay Chen ◽  
J. Thomas Roland ◽  
...  
Keyword(s):  
Cochlear Implant ◽  
Electrode Array ◽  
Cost Effective ◽  
X Rays ◽  
Normal Anatomy ◽  
Electrode Arrays ◽  
Plain Radiographs ◽  
Hearing Function ◽  
Tertiary Center ◽  
Cochlear Implant Surgery

Although malpositioning of the cochlear implant (CI) electrode array is rare in patients with normal anatomy, when occurring it may result in reduced hearing outcome. In addition to intraoperative electrophysiologic tests, imaging is an important modality to assess correct electrode array placement. The purpose of this report was to assess the incidence and describe cases in which intraoperative plain radiographs detected a malpositioned array. Intraoperative anti-Stenver’s view plain X-rays are conducted routinely in all CI surgeries in our tertiary center before awakening the patient and breaking the sterile field. Data of patients undergoing 399 CI surgeries were retrospectively analyzed. A total of 355 had normal inner ear and temporal bone anatomy. Patients with intra or extracochlear malpositioned electrode arrays demonstrated in the intraoperative X-ray were described. There were four cases of electrode array malposition out of 355 implantations with normal anatomy (1.1%): two tip fold-overs, one extracochlear placement and one partial insertion. All electrodes were reinserted immediately; repeated radiographs were normal and the patients achieved good hearing function. Intraoperative plain anti-Stenver’s view X-rays are valuable to confirm electrode array location, allowing correction before the conclusion of surgery. These radiographs are cheaper, faster, and emit much less radiation than other imaging options, making them a viable cost-effective tool in patients with normal anatomy.

Precise Alignment of Micromachined Electrode Arrays With V1 Functional Maps

2007 ◽  
Vol 97 (5) ◽  
pp. 3781-3789 ◽  
Author(s):  
Ian Nauhaus ◽  
Dario L. Ringach
Keyword(s):  
Receptive Field ◽  
Electrode Array ◽  
Theoretical Models ◽  
Orientation Tuning ◽  
Feedback Signal ◽  
Electrode Arrays ◽  
Tuning Curves ◽  
Alignment Procedure ◽  
Receptive Field Properties ◽  
Orientation Maps

Recent theoretical models of primary visual cortex predict a relationship between receptive field properties and the location of the neuron within the orientation maps. Testing these predictions requires the development of new methods that allow the recording of single units at various locations across the orientation map. Here we present a novel technique for the precise alignment of functional maps and array recordings. Our strategy consists of first measuring the orientation maps in V1 using intrinsic optical imaging. A micromachined electrode array is subsequently implanted in the same patch of cortex for electrophysiological recordings, including the measurement of orientation tuning curves. The location of the array within the map is obtained by finding the position that maximizes the agreement between the preferred orientations measured electrically and optically. Experimental results of the alignment procedure from two implementations in monkey V1 are presented. The estimated accuracy of the procedure is evaluated using computer simulations. The methodology should prove useful in studying how signals from the local neighborhood of a neuron, thought to provide a dominant feedback signal, shape the receptive field properties in V1.

scholarly journals Design and development of a multichannel potentiometer for monitoring an electrode array and its application in flow analysis

2002 ◽  
Vol 24 (4) ◽  
pp. 105-110 ◽  
Author(s):  
Jarbas J. R. Rohwedder ◽  
Celio Pasquini ◽  
Ivo M. Raimundo, Jr. ◽  
M. Conceiçao ◽  
B. S. M. Montenegro ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Flow Analysis ◽  
Reference Electrode ◽  
Electrode Array ◽  
Ion Selective Electrodes ◽  
Electrode Arrays ◽  
Figures Of Merit ◽  
Flow Through ◽  
The Individual ◽  
Intense Signal

A versatile potentiometer that works with electrode arrays in flow injection and/or monosegmented flow systems is described. The potentiometer is controlled by a microcomputer that allows individual, sequential multiplexed or random accesses to eight electrodes while employing only one reference electrode. The instrument was demonstrated by monitoring an array of seven flow-through ion-selective electrodes for Ag+and for three electrodes for Cl-, Ca2+and K+. The figures of merit of the individual and multiplexed (summed) readings of the electrode array were compared. The absolute standard deviation of the measurements made by summing the potential of two or more electrodes was maintained constant, thus improving the precision of the measurements. This result shows that an attempt to combine the signals of the electrodes to produce a more intense signal in the Hadamard strategy is feasible and accompanied by a proportional improvement in the precision of individual measurements. The preliminary tests suggest that the system can allow for 270 determinations per hour, with a linear range from1.0×10−2to1.0×10−4mol l-1for the three di¡erent analytes. Detection limits were estimated as3.1×10−5,3.0×10−6and1.0×10−5mol l-1for Cl-, Ca2+and K+, respectively.

scholarly journals CRSP, numerical results for an electrical resistivity array to detect underground cavities

2017 ◽  
Vol 9 (1) ◽  
Author(s):  
Amin Amini ◽  
Hamidreza Ramazi
Keyword(s):  
Electrical Resistivity ◽  
Geophysical Methods ◽  
Electrode Array ◽  
Electrode Configuration ◽  
Electrode Arrays ◽  
Wide Range ◽  
Underground Cavities ◽  
Conventional Electrode

AbstractThis paper is devoted to the application of the Combined Resistivity Sounding and Profiling electrode configuration (CRSP) to detect underground cavities. Electrical resistivity surveying is among the most favorite geophysical methods due to its nondestructive and economical properties in a wide range of geosciences. Several types of the electrode arrays are applied to detect different certain objectives. In one hand, the electrode array plays an important role in determination of output resolution and depth of investigations in all resistivity surveys. On the other hand, they have their own merits and demerits in terms of depth of investigations, signal strength, and sensitivity to resistivity variations. In this article several synthetic models, simulating different conditions of cavity occurrence, were used to examine the responses of some conventional electrode arrays and also CRSP array. The results showed that CRSP electrode configuration can detect the desired objectives with a higher resolution rather than some other types of arrays. Also a field case study was discussed in which electrical resistivity approach was conducted in Abshenasan expressway (Tehran, Iran) U-turn bridge site for detecting potential cavities and/or filling loose materials. The results led to detect an aqueduct tunnel passing beneath the study area.

Performance Evaluation of a Triangular-Prism-Slit Electrode Pair as an Electro-Conjugate Fluid Jet Generator

2011 ◽  
Author(s):  
Hai-bo Wang ◽  
Joon-wan Kim ◽  
Shinichi Yokota ◽  
Kazuya Edamura
Keyword(s):  
High Performance ◽  
Electrode Array ◽  
Research Field ◽  
Electrode Pair ◽  
Triangular Prism ◽  
Electrode Structure ◽  
Electrode Gap ◽  
Promising Candidate ◽  
Output Pressure ◽  
The Relationship

Electro-conjugate fluid (ECF) is a dielectric and functional fluid, which generates a powerful jet when electrodes inserted into it are subjected to a constant voltage of less than one thousand volts. As one essential research field on ECF, researchers have been conducting the study on electrodes. Several structures, e.g. planar parallel rod-like electrode array, ring-needle electrode pair and triangular-prism-slit (TPS) electrode pair, were proposed. Among them, the TPS electrode structure is often thought as the most promising candidate for future ECF applications thanks to its great merits of combining easy fabrication and relatively high performance. In this paper, in order to evaluate performance of the TPS electrode pair, a novel modular ECF-jet generator capable of independently adjusting alignment and gap of each electrode is designed. By utilizing it, the relationship between output pressure and parameters of the TPS electrode pair, including thickness, slit width, tip angle, electrode gap and alignment, are obtained.

Electrode design and insertional depth-dependent intra-cochlear pressure changes: a model experiment

2017 ◽  
Vol 132 (3) ◽  
pp. 224-229 ◽  
Author(s):  
P Mittmann ◽  
A Ernst ◽  
I Todt
Keyword(s):  
Cochlear Implant ◽  
Fluid Pressure ◽  
Electrode Array ◽  
Lateral Wall ◽  
Peak Frequency ◽  
Peak Amplitude ◽  
Residual Hearing ◽  
Electrode Arrays ◽  
Pressure Peak ◽  
Pressure Changes

AbstractBackground:Preservation of residual hearing is one of the major goals in modern cochlear implant surgery. Intra-cochlear fluid pressure changes influence residual hearing, and should be kept low before, during and after cochlear implant insertion.Methods:Experiments were performed in an artificial cochlear model. A pressure sensor was inserted in the apical part. Five insertions were performed on two electrode arrays. Each insertion was divided into three parts, and statistically evaluated in terms of pressure peak frequency and pressure peak amplitude.Results:The peak frequency over each third part of the electrode increased in both electrode arrays. A slight increase was seen in peak amplitude in the lateral wall electrode array, but not in the midscalar electrode array. Significant differences were found in the first third of both electrode arrays.Conclusion:The midscalar and lateral wall electrode arrays have different intra-cochlear fluid pressure changes associated with intra-cochlear placement, electrode characteristics and insertion.

Estimating depth of investigation in dc resistivity and IP surveys

Geophysics ◽  
1999 ◽  
Vol 64 (2) ◽  
pp. 403-416 ◽  
Author(s):  
Douglas W. Oldenburg ◽  
Yaoguo Li
Keyword(s):  
Electrode Array ◽  
Physical Property ◽  
Model Space ◽  
Visual Assessment ◽  
Order Estimate ◽  
Dc Resistivity ◽  
Electrode Arrays ◽  
The Earth ◽  
Surface Data ◽  
Depth Of Investigation

In this paper, the term “depth of investigation” refers generically to the depth below which surface data are insensitive to the value of the physical property of the earth. Estimates of this depth for dc resistivity and induced polarization (IP) surveys are essential when interpreting models obtained from any inversion because structure beneath that depth should not be interpreted geologically. We advocate carrying out a limited exploration of model space to generate a few models that have minimum structure and that differ substantially from the final model used for interpretation. Visual assessment of these models often provides answers about existence of deeper structures. Differences between the models can be quantified into a depth of investigation (DOI) index that can be displayed with the model used for interpretation. An explicit algorithm for evaluating the DOI is presented. The DOI curves are somewhat dependent upon the parameters used to generate the different models, but the results are robust enough to provide the user with a first‐order estimate of a depth region below which the earth structure is no longer constrained by the data. This prevents overinterpretation of the inversion results. The DOI analysis reaffirms the generally accepted conclusions that different electrode array geometries have different depths of penetration. However, the differences between the inverted models for different electrode arrays are far less than differences in the pseudosection images. Field data from the Century deposit are inverted and presented with their DOI index.

scholarly journals Graphene-based carbon-layered electrode array technology for neural imaging and optogenetic applications

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Dong-Wook Park ◽  
Amelia A. Schendel ◽  
Solomon Mikael ◽  
Sarah K. Brodnick ◽  
Thomas J. Richner ◽  
...  
Keyword(s):  
Soft Tissues ◽  
Electrode Array ◽  
Electrode Arrays ◽  
Neural Imaging ◽  
Brain Surface ◽  
Array Technology ◽  
Micro Electrode Arrays ◽  
The Brain

Abstract Neural micro-electrode arrays that are transparent over a broad wavelength spectrum from ultraviolet to infrared could allow for simultaneous electrophysiology and optical imaging, as well as optogenetic modulation of the underlying brain tissue. The long-term biocompatibility and reliability of neural micro-electrodes also require their mechanical flexibility and compliance with soft tissues. Here we present a graphene-based, carbon-layered electrode array (CLEAR) device, which can be implanted on the brain surface in rodents for high-resolution neurophysiological recording. We characterize optical transparency of the device at >90% transmission over the ultraviolet to infrared spectrum and demonstrate its utility through optical interface experiments that use this broad spectrum transparency. These include optogenetic activation of focal cortical areas directly beneath electrodes, in vivo imaging of the cortical vasculature via fluorescence microscopy and 3D optical coherence tomography. This study demonstrates an array of interfacing abilities of the CLEAR device and its utility for neural applications.

Area Effect of Bath Mode Micro Electro-Discharge Machining

2010 ◽  
Vol 431-432 ◽  
pp. 65-69
Author(s):  
Ping Mei Ming ◽  
Di Zhu ◽  
Yang Yang Hu ◽  
Yong Bin Zeng
Keyword(s):  
Electrode Pair ◽  
Electrode Wear ◽  
Micro Edm ◽  
Electrode Arrays ◽  
Shape Accuracy ◽  
Batch Mode ◽  
Positive Type ◽  
Area Effect ◽  
Negative Type ◽  
The Impact

This paper investigated the impact of area effect in the batch mode micro-EDM on shape accuracy and surface morphology of the machined microstructures, as well as electrode wear, using both the positive-type and the negative-type electrode arrays fabricated by the UV-LIGA process. Experimental results showed that ,with the increase of electrode-pair scale from Φ1mm, Φ2mm, Φ4mm to Φ8mm, the shape accuracy of the machined stainless steel microstructures increased, but the eroded microcavities of the machined area reduced in both the positive-type and the negative-type micro-EDM using the same electrical pulse parameters. It was also found that, with the same pulse energy and electrode-pair area, the electrode wear reduced as the electrode-couple area increased, and the wear ratio in the positive-type micro-EDM was larger than that in the negative-type micro-EDM.

Enhancement of Mixing in a Microchannel by Using AC-Electroosmotic Effect

2008 ◽  
Author(s):  
Yangyang Wang ◽  
Sangmo Kang ◽  
Yongkweon Suh
Keyword(s):  
Numerical Simulations ◽  
Chaotic Behavior ◽  
Electrode Array ◽  
Longitudinal Direction ◽  
Central Line ◽  
Transverse Flow ◽  
Electrode Pair ◽  
Mixing Performance ◽  
Commercial Code ◽  
Micro Mixer

This study has focused on optimizing the AC-electroosmotic micro-mixer, which is composed of a microchannel with an array of rectangular electrodes attached on the bottom wall. The electrode array is spatial-periodically arranged in pairs symmetric with respect to the longitudinal central line. An AC electrode field is applied to the electrodes, which drives the secondary transverse flow in a circulating cell mode near the electrodes. The main flow along the channel longitudinal direction plus this secondary transverse flow contribute to the stretching and folding of the fluid flow, that is the chaotic behavior, and thus to the enhancement of the fluid mixing. To design the better micro-mixer, numerical simulations have been performed by using a commercial code (CFX 10). In the simulations, the concept of mixing index is employed to evaluate the mixing performance as well as to optimize the size and spacing of each electrode in one pair. It is found that the optimum design of one electrode pair, which leads to the best mixing performance, is not simply harmonic one. When the length ratio of the two electrodes in a pair closes to 2:1, the best mixing effect can be attained. The flow pattern was visualized. Furthermore, the velocity field will be measured with a PTV technique to validate the numerical simulations.

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