scholarly journals Evaluation of Immunohistochemical Reactivity with Avidin-Biotin Complex (ABC) Method

2018 ◽  
Vol 3 (3) ◽  
pp. 909-918
Author(s):  
C. Sandoval ◽  
B. Vásquez
Keyword(s):  
Malignant Melanoma ◽  
Primary Antibody ◽  
Histopathological Diagnosis ◽  
Distilled Water ◽  
Correct Interpretation ◽  
S 100 ◽  
Antibody Reaction ◽  
Abc Method ◽  
Antigen Antibody ◽  
Avidin Biotin Complex

Immunohistochemistry is any technique that can detect cellular and extracellular components in situ by means of specific antibodies, using enzymatic detection systems. Among immunohistochemical methods, the technique of avidin - biotin complex (ABC) is widely used because of its high sensitivity. The aim of this study was to evaluate the immunohistochemical reactivity of the 4C4.9 antibody for detection of S-100 protein using the ABC method. For the evaluation of immunohistochemical reactivity 2 biopsies of human skin were used with histopathological diagnosis of ulcerated malignant melanoma and melanocytic intradermal nevi from the Research Laboratory on Animal Biotechnology of the Universidad de La Frontera, Chile. The Kit VECTASTAIN® was used as detection method, the dilution the 4C4.9 antibody was 1/250 and incubation temperature was at 4 ° C or 37 °C for 18 hours. To validate the technique, a positive control and a negative for 4C4.9 was performed. The results of immunohistochemical staining by the method of ABC complex showed positive staining for protein S-100 both in ulcerated malignant melanoma and melanocytic intradermal nevi, incubated for 18 hours at 4 ° C or 37 ° C. However, immunostaining was more intense when the primary antibody was incubated at 37° C. For a correct interpretation of the results, it is necessary to take into consideration that the antigen-antibody reaction is influenced by various factors such as the concentration of antibody, time and temperature of incubation. In conclusion, our results suggest incubating the samples with the first antibody (4C4.9) at 1/250 dilution in distilled water, incubating for 18 h at 37 oC. However, immunostaining was more intense when the primary antibody was incubated at 37° C. For a correct interpretation of the results, it is necessary to take into consideration that antigen- antibody reaction is influenced by various factors such as the concentration of antibody, time and temperature of incubation. In conclusion, our results suggest incubating the samples with the first antibody (4C4.9) at 1/250 dilution in distilled water, incubating for 18 h at 37 oC. The use of the antibody 4C4.9 is recommended to support the diagnosis and differential diagnosis.

scholarly journals Primary ovarian malignant melanoma

2010 ◽  
Vol 67 (3) ◽  
pp. 252-255
Author(s):  
Milos Kostov ◽  
Biserka Vukomanovic-Djurdjevic ◽  
Dane Nenadic ◽  
Milos Pavlovic
Keyword(s):  
Case Report ◽  
Malignant Melanoma ◽  
Primary Melanoma ◽  
Histopathological Diagnosis ◽  
Solid Mass ◽  
Diagnostic Problem ◽  
S 100 ◽  
Black Color ◽  
Immunohistochemical Analyses ◽  
Hmb 45

Background. Primary ovarian malignant melanoma is extremely rare. It usually appears in the wall of a dermoid cyst or is associated with another teratomatous component. Metastatic primary malignant melanoma to ovary from a primary melanoma elsewhere is well known and has been often reported especially in autopsy studies. Case report. We presented a case of primary ovarian malignant melanoma in a 45- year old woman, with no evidence of extraovarian primary melanoma nor teratomatous component. The tumor was unilateral, macroscopically on section presented as solid mass, dark brown to black color. Microscopically, tumor cells showed positive immunohistochemical reaction for HMB-45, melan-A and S-100 protein, and negative immunoreactivity for estrogen and progesteron receptors. Conclusion. Differentiate metastatic melanoma from rare primary ovarian malignant melanoma, in some of cases may be a histopathological diagnostic problem. Histopathological diagnosis of primary ovarian malignant melanoma should be confirmed by immunohistochemical analyses and detailed clinical search for an occult primary tumor.

Radioimmunodetection of Malignant Melanoma with the 225.28S Monoclonal Antibody to HMW-MAA

1986 ◽  
Vol 25 (06) ◽  
pp. 220-224 ◽  
Author(s):  
G. L. Buraggi
Keyword(s):  
Monoclonal Antibody ◽  
Pilot Study ◽  
Malignant Melanoma ◽  
Prospective Study ◽  
Clinical Application ◽  
Multicenter Trial ◽  
Clinical Applications ◽  
Gamma Energy ◽  
A Prospective Study ◽  
Antigen Antibody

A review of the studies on the use of the antigen-antibody system HMW-MAA 225.28S in melanoma radioimmunodetection is reported. The results obtained in a pilot study (42 patients with 74 lesions), a multicenter trial (254 patients with 553 lesions) and a prospective study still outstanding (29 patients with 38 lesions) allow to consider this system as suitable for clinical application. F(ab′)2 labelled with 99mTc gave the best results in terms of positivity. Moreover this radioisotope allows the best dosimetric conditions. The gamma energy emitted by this radionuclide is particularly convenient for conventional scintillation cameras and ECT. Very good results in terms of sensitivity (70%-85%) and especially specificity (about 100%) were achieved. Possible clinical applications of the method are discussed.

scholarly journals Is clear cell sarcoma a malignant form of psammomatous melanotic schwannoma?

2006 ◽  
Vol 21 (6) ◽  
pp. 1-4 ◽  
Author(s):  
Ahmet Şengöz ◽  
Erol Taşdemiroğlu ◽  
Halit Togay
Keyword(s):  
Malignant Melanoma ◽  
Nerve Root ◽  
Clear Cell ◽  
Clear Cell Sarcoma ◽  
Histopathological Diagnosis ◽  
Total Resection ◽  
Cell Sarcoma ◽  
Melanotic Schwannoma ◽  
Psammomatous Melanotic Schwannoma

✓The authors present a case of clear cell sarcoma (CCS) in which the tumor originated in the S-1 nerve root and had been previously diagnosed as psammomatous melanotic schwannoma (PMS). This is the third case of a spinal nerve root origin for CCS reported in the English-language literature. The similar histogenesis of CCS and malignant melanoma supports the hypothesis that biological agents or immunotherapy are potentially important areas of investigation. The patient underwent S1–3 laminectomy and gross-total resection of the mass lesion. The border of the resection was extended 1 cm distal to the tumor margin. The postoperative period was uneventful. The new histopathological diagnosis was CCS (malignant melanoma of soft tissue). Despite total resection, the patient returned with disseminated disease at the 18-month follow-up visit. His follow-up magnetic resonance image of the lumbar spine revealed sacral L5–S3 involvement of the vertebral bodies along with disseminated cauda equina seeding. A CCS originating from peripheral nerves is quite rare. The histopathological and immunohistochemical appearance of CCSs resembles those of PMSs. Surgery should be the first choice of treatment.

scholarly journals Evaluation of Shear Horizontal Surface Acoustic Wave Biosensors Using “Layer Parameter” Obtained from Sensor Responses during Immunoreaction

Sensors ◽  
2021 ◽  
Vol 21 (14) ◽  
pp. 4924
Author(s):  
Koji Kano ◽  
Hiromi Yatsuda ◽  
Jun Kondoh
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Horizontal Surface ◽  
Propagation Characteristics ◽  
Viscosity Change ◽  
Layer Parameter ◽  
Antibody Reaction ◽  
Antigen Antibody ◽  
Shear Horizontal ◽  
Molecular Dimensions

Shear horizontal surface acoustic wave (SH-SAW) biosensors measure the reaction of capture antibodies immobilized on the sensing surface to capture test molecules (antigens) by using the change in SH-SAW propagation characteristics. SH-SAW displacement exists not only on the SH-SAW propagating surface, but also partially penetrates the specimen liquid to a certain depth, which is determined by the liquid properties of the specimen and the operating frequency of the SH-SAW. This phenomenon is called viscosity penetration. In previous studies, the effect of viscosity penetration was not considered in the measurement of SH-SAW biosensors, and the mass or viscosity change caused by the specific binding of capture antibodies to the target antigen was mainly used for the measurement. However, by considering the effect of viscosity penetration, it was found that the antigen–antibody reaction could be measured and the detection characteristics of the biosensor could be improved. Therefore, this study aims to evaluate the detection properties of SH-SAW biosensors in the surface height direction by investigating the relationship between molecular dimensions and SH-SAW propagation characteristics, which are pseudo-changed by varying the diameter of gold nanoparticles. For the evaluation, we introduced a layer parameter defined by the ratio of the SH-SAW amplitude change to the SH-SAW velocity change caused by the antigen–antibody reaction. We found a correlation between the layer parameter and pseudo-varied molecular dimensions. The results suggest that SH-SAW does not only measure the mass and viscosity but can also measure the size of the molecule to be detected. This shows that SH-SAW biosensors can be used for advanced functionality.

scholarly journals A Case of Primary Subglottic Malignant Melanoma with a Successful Surgical Treatment

2014 ◽  
Vol 2014 ◽  
pp. 1-3
Author(s):  
Shahzad Ahmad ◽  
Mahmoud Abdelghany ◽  
Curtis Goldblatt ◽  
Owen Stark ◽  
Nicholas Masciotra
Keyword(s):  
Surgical Treatment ◽  
Malignant Melanoma ◽  
Excisional Biopsy ◽  
Human Melanoma ◽  
Primary Malignant Melanoma ◽  
Shortness Of Breath ◽  
S 100 ◽  
Hmb 45

Primary subglottic malignant melanoma is a very rare and underdiagnosed neoplasm. We are reporting a case of primary malignant melanoma of subglottic mucosa in a 78-year-old woman who presented to our hospital with shortness of breath and hoarseness of voice. Laryngoscopy and excisional biopsy along with immunoreactivity to S-100 and human melanoma black-45 (HMB-45) confirmed the diagnosis. The patient was treated with laryngectomy followed by radiotherapy. Five years following surgical treatment, she continues to be asymptomatic. To our knowledge, there is only one reported case of primary malignant melanoma of subglottic mucosa in the medical literatures.

Malignant Melanoma Presenting as an Intrathymic Tumor

2000 ◽  
Vol 124 (1) ◽  
pp. 130-134
Author(s):  
P. M. Alli ◽  
B. J. Crain ◽  
R. Heitmiller ◽  
P. Argani
Keyword(s):  
Malignant Melanoma ◽  
Previous History ◽  
Clinical History ◽  
Computed Tomographic ◽  
Eosinophilic Cytoplasm ◽  
S 100 ◽  
History Of ◽  
Solitary Metastases ◽  
Physical Findings ◽  
Pleomorphic Cells

Abstract The identification of malignant melanoma in a visceral organ of nonepidermal origin is not an uncommon occurrence. Frequently, these cases are solitary metastases that present years after a thin epidermal melanoma has been diagnosed (and sometimes forgotten). However, primary visceral melanomas have been reported that have not been preceded by an epidermal lesion. We describe herein a unique case of melanoma presenting as a primary intrathymic tumor. The patient had no previous history of epidermal melanoma, and extensive workup did not reveal evidence for an alternative primary site. The tumor exhibited histologic features characteristic of melanoma, including an abundance of large pleomorphic cells with eosinophilic cytoplasm, prominent nucleoli, and occasional intranuclear inclusions. Tumor cells stained for HMB-45 and S-100 protein and ultrastructural analysis revealed stage II and stage III melanosomes. The patient remained free of disease until intrathoracic recurrence was detected on a computed tomographic scan 14 months later. The lack of clinical history and physical findings of melanoma at presentation, the intrathymic location of the tumor, and the pattern of recurrence suggest that this case likely represents a primary thymic melanoma, a previously unreported entity.

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