scholarly journals Cystatin-SN

2020 ◽  
Author(s):  
Keyword(s):  
Cystatin Sn

scholarly journals Differences in Salivary Proteins as a Function of PROP Taster Status and Gender in Normal Weight and Obese Subjects

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2244
Author(s):  
Melania Melis ◽  
Mariano Mastinu ◽  
Stefano Pintus ◽  
Tiziana Cabras ◽  
Roberto Crnjar ◽  
...  
Keyword(s):  
Food Preferences ◽  
Specific Weight ◽  
Normal Weight ◽  
Nutrition Status ◽  
Salivary Proteins ◽  
Specific Proteins ◽  
Obese Subjects ◽  
And Gender ◽  
Cystatin Sn

Taste plays an important role in processes such as food choices, nutrition status and health. Salivary proteins contribute to taste sensitivity. Taste reduction has been associated with obesity. Gender influences the obesity predisposition and the genetic ability to perceive the bitterness of 6-n-propylthiouracil (PROP), oral marker for food preferences and consumption. We investigated variations in the profile of salivary proteome, analyzed by HPLC-ESI-MS, between sixty-one normal weight subjects (NW) and fifty-seven subjects with obesity (OB), based on gender and PROP sensitivity. Results showed variations of taste-related salivary proteins between NW and OB, which were differently associated with gender and PROP sensitivity. High levels of Ps-1, II-2 and IB-1 proteins belonging to basic proline rich proteins (bPRPs) and PRP-1 protein belonging to acid proline rich proteins (aPRPs) were found in OB males, who showed a lower body mass index (BMI) than OB females. High levels of Ps-1 protein and Cystatin SN (Cyst SN) were found in OB non-tasters, who had lower BMI than OB super-tasters. These new insights on the role of salivary proteins as a factor driving the specific weight gain of OB females and super-tasters, suggest the use of specific proteins as a strategic tool modifying taste responses related to eating behavior.

scholarly journals Resistance and resilience to experimental gingivitis: a systematic scoping review

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Charifa Zemouri ◽  
Nicholas S. Jakubovics ◽  
Wim Crielaard ◽  
Egija Zaura ◽  
Michael Dodds ◽  
...  
Keyword(s):  
Scoping Review ◽  
Gingival Crevicular Fluid ◽  
Oral Microbiome ◽  
Induction Phase ◽  
Experimental Gingivitis ◽  
Normal Composition ◽  
Baseline Values ◽  
Cystatin Sn ◽  
Crevicular Fluid ◽  
Reporting Data

Abstract Background This systematic scoping review aimed to identify changes in biomarkers of microbiological, immunological and biochemical origin during experimental gingivitis (EG) studies that might indicate resistance and resilience. Methods The term ‘experimental gingivitis’ was run in PubMed from inception to April 11th, 2018. From the 411 studies retrieved, 22 studies were included for this review. Results Studies reporting data on biomarker changes during and after full mouth EG trial were included. Two studies reported findings on changes in biomarkers of microbiological, 12 on immunological and eight on biochemical origin. Changes were reported in the induction phase, and occasionally in the resolution phase. The microbiological composition of both supragingival and subgingival dental plaque changed over the course of EG to a more pathogenic direction, but showed a shift back to a more normal composition. This indicates resilience of the oral microbiome. For immunological biomarkers, it was challenging to retrieve a robust pattern of changes across multiple studies. IL-1β and IL-6 in saliva and in gingival crevicular fluid increased during induction phase and returned in the resolution phase below baseline values. The biochemical parameters cystatin-SN, cystatin-S and lactoferrin in saliva were increased at the end of induction phase, however also here no clear pattern emerged based on all available studies. Conclusions More research is needed to investigate which microbiological, immunological, and biochemical biomarkers can be useful for future investigations into the resistance and resilience of the oral cavity to experimental gingivitis.

scholarly journals Research progress of cystatin SN in cancer

2019 ◽  
Vol Volume 12 ◽  
pp. 3411-3419 ◽  
Author(s):  
Yanfang Liu ◽  
Jing Yao
Keyword(s):  
Research Progress ◽  
Cystatin Sn

Biological activities and secondary structures of variant forms of human salivary cystatin SN produced in Escherichia coli

Gene ◽  
1994 ◽  
Vol 151 (1-2) ◽  
pp. 303-308 ◽  
Author(s):  
Libuse A. Bobek ◽  
Narayanan Ramasubbu ◽  
Xiahong Wang ◽  
Tara R. Weaver ◽  
Michael J. Levine
Keyword(s):  
Escherichia Coli ◽  
Biological Activities ◽  
Secondary Structures ◽  
Cystatin Sn

scholarly journals Expression of Human Salivary Histatin and Cystatin/ Histatin Chimeric cDNAs in Escherichia coli

1993 ◽  
Vol 4 (3) ◽  
pp. 581-590 ◽  
Author(s):  
L.A. Bobek ◽  
H. Tsai ◽  
M.J. Levine
Keyword(s):  
Escherichia Coli ◽  
Fusion Proteins ◽  
Expression System ◽  
Full Length ◽  
Blue Staining ◽  
Coomassie Blue Staining ◽  
Coomassie Blue ◽  
Overlap Extension ◽  
Cystatin Sn ◽  
The Individual

We have previously constructed recombinants encoding the full-length and truncated forms of cystatin-SN and expressed these in the Escherichia coli expression system pGEX-2T, which expresses foreign sequences as fusion proteins with glutathione S-transferase (GST). Recombinant cystatins were produced and purified in large quantities. The full-length recombinant cystatin-SN exhibited comparable biological activity and secondary structure to natural cystatin, validating the use of the full-length and mutant recombinant proteins for structure-function studies of salivary molecules. In this study, we have expressed histatin-1 cDNA in the pGEX-3X vector and cystatin-SN/histatin-1 or cystatin-SN/histatin-3 chimeric cDNAs in the pGEX-2T vector. Gene splicing by overlap extension (SOE), a PCR-based method, was used for generating the chimeric cDNAs. Each construct was analyzed by DNA sequencing, which showed the correct junctions and reading frames between the GST/histatin-1 and the GST/cystatin/histatin cDNAs. Expression of histatin and cystatin/histatin chimeras was induced by IPTG and the production of the fusion proteins monitored by SDS-PAGE/Coomassie blue staining and in the case of the GST/cystatin/histatin fusion proteins, also by Western blot using anti-cystatin antibody. The results of these studies showed that we have successfully constructed recombinants encoding the individual and chimeric salivary molecules and efficiently expressed these in E. coli expression system pGEX. Purification and characterization of recombinant histatin and cystatin-histatin hybrid proteins are presently ongoing.

scholarly journals Prognostic significance of cystatin SN associated nomograms in patients with colorectal cancer

Oncotarget ◽  
2017 ◽  
Vol 8 (70) ◽  
pp. 115153-115163
Author(s):  
Taiyuan Li ◽  
Qiangqiang Xiong ◽  
Zhen Zou ◽  
Xiong Lei ◽  
Qunguang Jiang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Prognostic Significance ◽  
Cystatin Sn
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