scholarly journals Physical Map of the Human Genome

2020 ◽  
Author(s):  
Keyword(s):  
Human Genome ◽  
Physical Map

scholarly journals A test case for physical mapping of human genome by repetitive sequence fingerprints: construction of a physical map of a 420 kb YAC subcloned into cosmids

1991 ◽  
Vol 19 (3) ◽  
pp. 505-510 ◽  
Author(s):  
C. Bellanné-Chantelot ◽  
E. Barillot ◽  
B. Lacroix ◽  
D.Le Paslier ◽  
D. Cohen
Keyword(s):  
Human Genome ◽  
Physical Mapping ◽  
Repetitive Sequence ◽  
Physical Map ◽  
Test Case

A first-generation physical map of the human genome

Nature ◽  
1993 ◽  
Vol 366 (6456) ◽  
pp. 698-701 ◽  
Author(s):  
D. Cohen ◽  
I. Chumakov ◽  
J. Weissenbach
Keyword(s):  
Human Genome ◽  
First Generation ◽  
Physical Map

Piggy-BACing the Human Genome I: Constructing a Porcine BAC Physical Map Through Comparative Genomics

2008 ◽  
Vol 19 (1) ◽  
pp. 28-42 ◽  
Author(s):  
Margarita B. Rogatcheva ◽  
Kefei Chen ◽  
Denis M. Larkin ◽  
Stacey N. Meyers ◽  
Brandy M. Marron ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Human Genome ◽  
Physical Map

scholarly journals A physical map of the human genome

Nature ◽  
2001 ◽  
Vol 409 (6822) ◽  
pp. 934-941 ◽  
Author(s):  
Keyword(s):  
Human Genome ◽  
Physical Map

Pulsed-field electrophoresis of megabase-sized DNA

1991 ◽  
Vol 11 (6) ◽  
pp. 3348-3354
Author(s):  
K Gunderson ◽  
G Chu
Keyword(s):  
Human Genome ◽  
Physical Map ◽  
Theoretical Models ◽  
Pulsed Field ◽  
Practical Applications ◽  
Lateral Band ◽  
Systematic Exploration ◽  
Pulsed Field Electrophoresis ◽  
Pulsed Fields ◽  
Band Spreading

Success in constructing a physical map of the human genome will depend on two capabilities: rapid resolution of very large DNA and identification of migration anomalies. To address these issues, a systematic exploration of pulsed-field electrophoresis conditions for separating multimegabase-sized DNA was undertaken. Conditions were found for first liberating and then separating DNA up to 6 megabases at higher field strengths and more rapidly than previously reported. In addition, some conditions for transversely pulsed fields produced mobility inversion, in which increased size was accompanied by faster rather than slower migration. Importantly, anomalous migration could be identified by the presence of lateral band spreading, in which the DNA band remained sharply defined but spread laterally while moving down the gel. These results have implications for both practical applications and theoretical models of pulsed-field electrophoresis.

scholarly journals Assembly, Annotation, and Integration of UNIGENE Clusters into the Human Genome Draft

2001 ◽  
Vol 11 (5) ◽  
pp. 904-918
Author(s):  
Degen Zhuo ◽  
Wei D. Zhao ◽  
Fred A. Wright ◽  
Hee-Yung Yang ◽  
Jian-Ping Wang ◽  
...  
Keyword(s):  
Human Genome ◽  
Positional Cloning ◽  
Physical Map ◽  
Sequence Contigs ◽  
New Genes ◽  
Human Genes ◽  
Individual Transcript ◽  
Genome Draft ◽  
Human Transcript ◽  
The Individual

The recent release of the first draft of the human genome provides an unprecedented opportunity to integrate human genes and their functions in a complete positional context. However, at least three significant technical hurdles remain: first, to assemble a complete and nonredundant human transcript index; second, to accurately place the individual transcript indices on the human genome; and third, to functionally annotate all human genes. Here, we report the extension of the UNIGENE database through the assembly of its sequence clusters into nonredundant sequence contigs. Each resulting consensus was aligned to the human genome draft. A unique location for each transcript within the human genome was determined by the integration of the restriction fingerprint, assembled genomic contig, and radiation hybrid (RH) maps. A total of 59,500 UNIGENE clusters were mapped on the basis of at least three independent criteria as compared with the 30,000 human genes/ESTs currently mapped in Genemap'99. Finally, the extension of the human transcript consensus in this study enabled a greater number of putative functional assignments than the 11,000 annotated entries in UNIGENE. This study reports a draft physical map with annotations for a majority of the human transcripts, called the Human Index of Nonredundant Transcripts (HINT). Such information can be immediately applied to the discovery of new genes and the identification of candidate genes for positional cloning.

scholarly journals A second-generation combined linkage physical map of the human genome

2007 ◽  
Vol 17 (12) ◽  
pp. 1783-1786 ◽  
Author(s):  
T. C. Matise ◽  
F. Chen ◽  
W. Chen ◽  
F. M. De La Vega ◽  
M. Hansen ◽  
...  
Keyword(s):  
Human Genome ◽  
Second Generation ◽  
Physical Map

scholarly journals Nematode Functional Genomics

Yeast ◽  
2000 ◽  
Vol 1 (1) ◽  
pp. 43-47
Keyword(s):  
Functional Genomics ◽  
Human Genome ◽  
Genome Sequence ◽  
Protein Interactions ◽  
Dna Microarrays ◽  
Molecular Mechanisms ◽  
Physical Map ◽  
Cell Signalling ◽  
Genome Project ◽  
C Elegans

Alan Coulson has two main roles at the Sanger Centre, revolving around the worm and the human genome projects. Although the worm sequence is essentially finished, the tidying-up of that and the physical map is ongoing. There is also a continuous need for communication with the worm field with regard to information and materials relating to the sequence project. For example, the cosmids and YACs of the physical map continue to be, as they have been for many years now, an extremely powerful resource, and the Sanger Centre distributes in the order of 500 clones per month to the community.Alan is team leader of the worm functional genomics group, which is currently small but will be expanding shortly. Patricia Kuwabara is a member of the team and a description of their activities can be found below. The Human Genome Project is sequencing mapped PAC and BAC clones. Alan's primary involvement is with the team that is responsible for subcloning the 10 000 or so clones that will be required to complete the one-third of the genome sequence to be contributed by the Sanger Centre.Patricia Kuwabara has been using Caenorhabditis elegans as a model for understanding how protein–protein interactions regulate cell-to-cell signalling. Her research has focused on understanding the molecular mechanisms underlying the genetics of C. elegans sex determination. This work has led into a study of regulated proteolysis involving calpains and also into the roles of the multiple C. elegans Patched proteins, which in other organisms have been shown to be receptors for the Hedgehog morphogen.In addition, the group is taking advantage of the completion of the C. elegans genome sequence to develop whole genome DNA microarrays for expression profiling. At the Sanger Centre, DNA microarrays are providing opportunities to examine how development and physiology are regulated globally, because most nematode genes have now been identified at the sequence level. The group are being assisted in this endeavour by Dr Stuart Kim (Stanford, CA).

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