scholarly journals Parinaric Acid

2020 ◽  
Author(s):  
Keyword(s):  
Parinaric Acid

[34] Parinaric acid from Parinarium glaberrimum

1981 ◽  
pp. 479-482 ◽  
Author(s):  
Larry A. Sklar ◽  
Bruce S. Hudson ◽  
Robert D. Simoni
Keyword(s):  
Parinaric Acid

Induced chirality upon binding ofcis-parinaric acid to bovine β-lactoglobulin: spectroscopic characterization of the complex

FEBS Letters ◽  
2002 ◽  
Vol 520 (1-3) ◽  
pp. 81-87 ◽  
Author(s):  
Ferenc Zsila ◽  
Tı́mea Imre ◽  
Pál T. Szabó ◽  
Zsolt Bikádi ◽  
Miklós Simonyi
Keyword(s):  
Spectroscopic Characterization ◽  
Parinaric Acid ◽  
Β Lactoglobulin

Synthesis of four isomers of parinaric acid

2004 ◽  
Vol 131 (2) ◽  
pp. 215-222 ◽  
Author(s):  
Dmitry V. Kuklev ◽  
William L. Smith
Keyword(s):  
Parinaric Acid

Quantitative Analysis of Phospholipid Peroxidation and Antioxidant Protection in Live Human Epidermal Keratinocytes

2001 ◽  
Vol 21 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Anna A. Shvedova ◽  
Yulia Y. Tyurina ◽  
Vladimir A. Tyurina ◽  
Yoko Kikuchi ◽  
Valerian E. Kagan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Quantitative Analysis ◽  
Phospholipid Composition ◽  
Peroxyl Radicals ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Normal Human Epidermal Keratinocytes ◽  
Normal Human ◽  
Parinaric Acid ◽  
Phospholipid Peroxidation

To characterize oxidative stress in phospholipids of normal human epidermal keratinocytes we metabolically labeled their membrane phospholipids with a natural oxidation-sensitive fluorescent fatty acid, cis-parinaric acid, and exposed the cells to two different sources of oxidants–a lipid-soluble azo-initiator of peroxyl radicals, 2,2′-azobis(2,4-dimethyl-valeronitrile), AMVN, and a superoxide generator, xanthine oxidase/xanthine. We demonstrated that both oxidants induced pronounced oxidation of four major classes of cis-parinaric acid-labeled phospholipids–phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol–in normal human epidermal keratinocytes that was not detectable as any significant change of their phospholipid composition. Vitamin E was effective in protecting the cells against phospholipid peroxidation. Since viability of normal human epidermal keratinocytes was not changed either by labeling or exposure to oxidants the labeling protocol and oxidative stress employed are compatible with the quantitative analysis of phospholipid peroxidation in viable cells.

Fluorescence polarization studies of rat liver microsomes with altered phospholipid desaturase activities

1980 ◽  
Vol 58 (10) ◽  
pp. 952-958 ◽  
Author(s):  
E. L. Pugh ◽  
M. Kates ◽  
A. G. Szabo
Keyword(s):  
Fatty Acid ◽  
Rat Liver ◽  
Fluorescence Polarization ◽  
Desaturase Activity ◽  
Liver Microsomes ◽  
Rat Liver Microsomes ◽  
Polarization Ratio ◽  
Parinaric Acid ◽  
Cis And Trans ◽  
Normal Controls

Phospholipid desaturase activity of rat liver microsomes can be varied by dietary manipulation: rats starved and refed a "fat-free" diet have two to three times the activity of normal controls whereas starved rats have no detectable activity. These changes were accompanied by changes in fatty acid composition of the microsomal phospholipids, resulting in a double bond:saturated fatty acid mole ratio (moles double bonds per mole saturated fatty acid) of 5.7 in control and starved rats and 4.7 in starved–refed rats. Fluorescence polarization ratio [Formula: see text] of cis- and trans-parinaric acid (PnA) showed no significant differences in physical state of the three microsomal preparations. However, the isolated microsomal phospholipids with trans-PnA as probe showed differences in the temperature at which onset of a change in polarization ratio occurred (starved–refed > normal > starved rats). With the cis-PnA as probe, the polarization ratio showed no change in the range 10–40 °C but was significantly higher (1.8) in starved–refed rats than in normal and starved rats (1.6 in both cases). These data indicate that the microsomal phospholipids of starved–refed animals were in a less fluid state than those from control and starved rats and that this decrease in fluidity was correlated with an increase in phospholipid desaturase activity.

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