scholarly journals Manual Count

2020 ◽  
Author(s):  
Keyword(s):  
Manual Count

A Critical Evaluation of Automatic Bacterial Colony Counters

1979 ◽  
Vol 42 (2) ◽  
pp. 138-143 ◽  
Author(s):  
M. H. BRODSKY ◽  
B. W. CIEBIN ◽  
D. A. SCHIEMANN
Keyword(s):  
Experimental Design ◽  
Critical Evaluation ◽  
Bacterial Colony ◽  
Pasteurized Milk ◽  
Instrument Calibration ◽  
Manual Count ◽  
Milk Samples ◽  
Precision And Accuracy ◽  
And Performance ◽  
Acceptability Criterion

Four automatic colony counters (ACC), 3M Model 620 and Artek Models 480, 870 and 880, were evaluated for their precision and accuracy in counting bacterial colonies in pour-plates prepared using raw and pasteurized milk samples. The automatic colony counters were precise, labor saving devices. but not one of the ACC units approached our acceptability criterion that 90% of the ACC counts fall within ± 10% of the corresponding manual count. Some parameters of experimental design and instrument calibration which may significantly influence the response and performance of the automatic counters are discussed.

Performance of automated digital cell imaging analyzer Sysmex DI-60

2017 ◽  
Vol 56 (1) ◽  
Author(s):  
Hyeong Nyeon Kim ◽  
Mina Hur ◽  
Hanah Kim ◽  
Seung Wan Kim ◽  
Hee-Won Moon ◽  
...  
Keyword(s):  
Platelet Count ◽  
Blood Cell ◽  
White Blood Cell ◽  
Red Blood Cell ◽  
Cell Imaging ◽  
Platelet Counts ◽  
Manual Count ◽  
Manual Counting ◽  
Highly Correlated

AbstractBackground:The Sysmex DI-60 system (DI-60, Sysmex, Kobe, Japan) is a new automated digital cell imaging analyzer. We explored the performance of DI-60 in comparison with Sysmex XN analyzer (XN, Sysmex) and manual count.Methods:In a total of 276 samples (176 abnormal and 100 normal samples), white blood cell (WBC) differentials, red blood cell (RBC) classification and platelet (PLT) estimation by DI-60 were compared with the results by XN and/or manual count. RBC morphology between pre-classification and verification was compared according to the ICSH grading criteria. The manual count was performed according to the Clinical and Laboratory Standards Institute guidelines (H20-A2).Results:The overall concordance between DI-60 and manual count for WBCs was 86.0%. The agreement between DI-60 pre-classification and verification was excellent (weighted κ=0.963) for WBC five-part differentials. The correlation with manual count was very strong for neutrophils (r=0.955), lymphocytes (r=0.871), immature granulocytes (r=0.820), and blasts (r=0.879). RBC grading showed notable differences between DI-60 and manual counting on the basis of the ICSH grading criteria. Platelet count by DI-60 highly correlated with that by XN (r=0.945). However, DI-60 underestimated platelet counts in samples with marked thrombocytosis.Conclusions:The performance of DI-60 for WBC differential, RBC classification, and platelet estimation seems to be acceptable even in abnormal samples with improvement after verification. DI-60 would help optimize the workflow in hematology laboratory with reduced manual workload.

scholarly journals Comparison of automated flowcytometric reticulocyte analysis with manual reticulocyte count

2019 ◽  
Vol 7 (10) ◽  
pp. 3825 ◽  
Author(s):  
Komal Patel ◽  
Siddhi M. Patel
Keyword(s):  
Bone Marrow ◽  
Reticulocyte Count ◽  
Good Method ◽  
Individual Case ◽  
Manual Count ◽  
Automated Method ◽  
Quality Control Parameters ◽  
Hematology Analyzer ◽  
The Mean ◽  
Automated Hematology Analyzer

Background: Reticulocytes are young or immature red blood cells released from bone marrow and that contain remanants of ribonucleic acid (RNA) and ribosomes. Reticulocyte count (RC) is the index of erythropoietic activity within bone marrow. The reticulocyte counting methods at clinical laboratories are currently divided into manual and automated.Methods: A total of 500 samples of study cases were processed by manual method using New Methylene Blue (NMB) and automated method based on flowcytometry by PENTRA XLR HORIBA hematology analyzer. All quality control parameters were evaluated and values obtained by both methods were compared using various statistical methods.Results: Automated hematology analyzer provides excellent precision and linearity with no significant carryover. On comparing manual and automated RC method good method correlation was found (correlation coefficient r-0.865), however individual case wise percent deviation between manual and automated RC and CRC varied significantly. In addition within run precision calculated for automated RC differed significantly from manual count. The mean of difference between duplicate readings (150 samples) of manual and automated RC (<5%) were 0.3 and 0.01 respectively while 6.3 and 0.15 respectively for >5% RC. Thus, automated method was found to be more precise than the manual RC.Conclusions: The manual count method for RC associated with significant imprecision compared to flowcytometric method mostly based on interobserver variation and the smaller number of cell being counted. In contrast, the automated method is rapid, easy to operate, count higher number of cells with precise measurement.

scholarly journals Penerapan Pathfinding Menggunakan Algoritma A* Pada Non Player Character (NPC) Di Game

2019 ◽  
Vol 17 (2) ◽  
pp. 39
Author(s):  
Paulus Harsadi ◽  
Sri Siswanti
Keyword(s):  
Optimal Solution ◽  
Design And Development ◽  
A Algorithm ◽  
Manual Count ◽  
Additional Information ◽  
Unity 3D ◽  
Adventure Game ◽  
Algorithm Implementation ◽  
Test Result ◽  
The Way

In this Game , we implement a character designer at the enemy by applying the algorithm A * pathfinding Algorithm. Furthermore, A * is as the algorithm for searching solutions with the use of additional information (heuristics) in which it is an optimal solution. The purpose of developing this Adventure Game  is A * Algorithm implementation for Pathfinding using Unity 3D. Pathfinding is the fastest path of searching process from point of origin to point of destination by avoiding the various barriers along the path traveled without crashing the existing barrier along the way. Its design and development applies Unity 3D. The test result of algorithm in the Game  shows that the total of passed node result is 50 nodes. It is similar with the previous manual count result. Furthermore, the Game  route shows that its passed path is similar with manual count. 

Vascular enumeration as a significant prognosticator for invasive breast carcinoma.

1998 ◽  
Vol 16 (5) ◽  
pp. 1684-1688 ◽  
Author(s):  
M J Aceñero ◽  
J F González ◽  
M G Gallego ◽  
P A Ballesteros
Keyword(s):  
Ductal Carcinoma ◽  
Univariate Analysis ◽  
Radical Mastectomy ◽  
Image Analyzer ◽  
Free Survival ◽  
Node Negative ◽  
Manual Count ◽  
Node Metastases ◽  
Vessel Count

PURPOSE Vascular enumeration has been claimed to be an independent prognosticator for invasive breast cancer. Most of the studies have performed a manual count of the vessels. Few investigators have used image analyzers to reduce subjectivity in the measures. The aim of this retrospective study was to compare the manual vessel count to the counts obtained with an image analyzer and to estimate their possible prognostic influence. MATERIALS AND METHODS We selected 112 patients with invasive ductal carcinoma, treated with radical mastectomy followed by chemotherapy and with a minimal follow-up time of 60 months (5 years). After immunostaining with factor VIII, we performed a double count of the vessels. First, we performed a manual count following Gasparini's criteria, and second, we used an image analyzer (Microm, Barcelona, Spain) to count the vessels in eight adjacent microscopic fields with a 20x objective, to reach a total area of 0.98 mm2. The image analyzer calculated the total number of vessels, their size and also the percentage of the field occupied by the vessels. RESULTS In our series, vascular enumeration showed no significant association with histologic grade (Bloom-Scarff-Richardson grading), tumor size, or staging. CONCLUSION Vascular enumeration with both methods was an independent prognosticator for relapse-free-survival (RFS) in both node-negative and -positive patients in the univariate analysis, but only vascular enumeration with the image analyzer was an independent prognostic factor in the multivariate analysis, together with lymph node metastases.

Comparing Three Methods for Counting Raw-Milk Pour Plates1

1979 ◽  
Vol 42 (9) ◽  
pp. 729-731
Author(s):  
J. A. WESLEY ◽  
B. E. LANGLOIS ◽  
J. O'LEARY
Keyword(s):  
Raw Milk ◽  
Regression Equations ◽  
Standard Methods ◽  
Manual Count ◽  
Milk Samples ◽  
Plate Counts ◽  
Electronic Probe ◽  
The Difference ◽  
Incubation Temperatures ◽  
Colony Counter

Six hundred Grade A raw milk samples were plated on Standard Methods agar and the pour-plate counts compared using three methods. Counts of each sample were determined after incubation at 26, 30 and 32 C for 72 h with an automatic colony counter (ACC). Quebec colony counter and hand tally (HT) and Quebec colony counter and electronic probe with digital register (DT). No significant differences were found between the counts obtained with the HT and the DT for any of the three incubation temperatures. The ACC counts were lower (P &lt; .01) than the manual counts at each temperature. The ACC counts were .39, .35 and .31 log counts lower than the manual count at 26, 30 and 32 C, respectively. The ACC counts at 72 h were higher (P &lt; .01) than the ACC counts at 48 h for all temperatures. The difference was about .41 log counts. The two manual counts were totally correlated, while the correlation between the ACC counts and the manual count; was 97. Regression equations were formulated for predicting the 72 h HT count from either the 48 or 72 h ACC count.

scholarly journals Analysis of Transmission Tower using Stadd Pro

2020 ◽  
Vol 9 (5) ◽  
pp. 1285-1289
Keyword(s):  
Wind Speed ◽  
3D Model ◽  
Damping Ratio ◽  
Landscape Classification ◽  
Transmission Tower ◽  
Wind Force ◽  
Source Power ◽  
Manual Count ◽  
High Transmission

Present days there are a lot of interest for power in the private and open parts in India has been expanded and to full fill, the interest of power a few activities is being taken by the legislature of India. From the source power to the customer the transmission tower is utilized as a transport framework. Right now, endeavor has been made to investigation a 36 m high transmission tower 3D model with the assistance of STAAD Pro (V8i) programming utilizing the codal arrangements of IS 800:2007 and furthermore the manual count of every part are determined for the examination as per codal arrangements.As per IS 875 (part III) code, the wind zone 3 height 36 m, landscape classification 2 and fundamental wind speed 39 m/sec are considered for the analysis. The damping ratio is 0.05 is also considered in the analysis. The results are concluded in terms of stability against wind force, structural, statically stable of all steel components.

Prediction of inventory sustainability during a drug shortage

2016 ◽  
Vol 73 (14) ◽  
pp. 1094-1098
Author(s):  
Nathaniel J. Rhodes ◽  
Elise M. Gilbert ◽  
Erik Skoglund ◽  
John S. Esterly ◽  
Michael J. Postelnick ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Consumption Rate ◽  
Medication Administration ◽  
Single Site ◽  
Drug Shortage ◽  
Evaluation Period ◽  
Manual Count ◽  
Review Period ◽  
Future Supply ◽  
The Mean

Abstract Purpose A methodology for predicting how long the on-hand inventory of a given medication will last during a supply shortage is described; a practical example of application of the methodology is provided. Methods Single-site data on consumption of i.v. tobramycin over an eight-month evaluation period were collected using commercial software that tabulates barcode-assisted medication administration (BCMA) events; administered doses were standardized as 1200-mg “vial-equivalents” and summed over the review period. The total number of vial-equivalents consumed was divided by the number of “non-zero weeks of consumption” (i.e., weeks during which any tobramycin use occurred) to obtain a mean ± S.D. weekly consumption rate; this rate was multiplied by the total i.v. tobramycin on-hand supply (in vial-equivalents) to determine the mean number of potentially sustainable weeks of therapy in the event a shortage were to restrict the future supply of the drug. Results Overall, 99.6 vial-equivalents of i.v. tobramycin were used during the evaluation period. The mean ± S.D. number of vial-equivalents used per non-zero week of consumption was 3.11 ± 1.26. A manual count of pharmacy inventory revealed that 102.9 vial-equivalents were available at the time of analysis. The mean predicted duration of supply was 33 weeks (95% confidence interval, –126 to 192 weeks). Conclusion Available BCMA data on tobramycin consumption over eight months were used to calculate the mean number of weeks the on-hand supply of the drug could be expected to last during a persistent drug shortage.

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