scholarly journals Phenotypic Multiple Antigen Bead-based Multiplex Assay

2020 ◽  
Author(s):  
Keyword(s):  
Multiplex Assay ◽  
Multiple Antigen

scholarly journals Implementation and evaluation of a novel real-time multiplex assay for SARS-CoV-2: in-field learnings from a clinical microbiology laboratory

Pathology ◽  
2020 ◽  
Vol 52 (7) ◽  
pp. 754-759 ◽  
Author(s):  
Eloise Williams ◽  
Katherine Bond ◽  
Brian Chong ◽  
Dawn Giltrap ◽  
Malcolm Eaton ◽  
...  
Keyword(s):  
Real Time ◽  
Clinical Microbiology ◽  
Multiplex Assay ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory

scholarly journals Immunogenicity of NS4b Dengue 3 Virus Mimotope Presented to the Immune System as Multiple Antigen Peptide System

ISRN Virology ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Nevis Amin ◽  
Maritza Pupo ◽  
Alicia Aguilar ◽  
Frank Camacho ◽  
Mayling Alvarez ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Vaccine Design ◽  
Amino Acid Sequences ◽  
Diagnostic Assays ◽  
Random Peptide ◽  
Multiple Antigen ◽  
Antigen Peptide ◽  
Multiple Antigen Peptide ◽  
Human Sera ◽  
Phage Displayed Peptide Library

The availability of random peptide libraries displayed on bacteriophage (RPL) has provided a powerful tool for selecting sequences that mimic binding properties of natural antigen epitopes (mimotopes). These mimotopes can be used for vaccine design, drug development, and diagnostic assays. Several mimotopes have been shown to induce production of antibodies against the natural antigen. We have previously identified four dengue virus mimotopes from a phage-displayed peptide library using antidengue 3 human sera. Three of them showed similarity in their amino acid sequences with the NS4b proteins of dengue. Few studies have examined the role of NS4b proteins in the antibody response to dengue virus infection. A multiple antigen peptide (MAP) system was chemically synthesized containing this mimotope (NS4b MAP), and BALB/c mice were immunized to evaluate its immunogenicity. Antipeptide responses were induced and recognised DENV-3 infected cells as determined by immunofluorescence. The high levels of the IgG2a subtype against NS4bMAP suggest the induction of a Th1-like response. Our findings suggest that the NS4b mimotope might be a useful tool for the development of multiepitope diagnostic assays, dengue virus vaccine design, and pathogenesis studies.

Structural probing of human lutropin using antibodies raised against synthetic peptides constructed by classical and multiple antigen peptide system approaches

1990 ◽  
Vol 27 (4) ◽  
pp. 363-368 ◽  
Author(s):  
Frédéric Troalen ◽  
Alain Razafindratsita ◽  
Alain Puisieux ◽  
Thibault Voeltzel ◽  
Claude Bohuon ◽  
...  
Keyword(s):  
Synthetic Peptides ◽  
Multiple Antigen ◽  
Antigen Peptide ◽  
Multiple Antigen Peptide ◽  
Peptide System ◽  
System Approaches

scholarly journals Expanding Assay Dynamics: A Combined Competitive and Direct Assay System for the Quantification of Proteins in Multiplexed Immunoassays

2008 ◽  
Vol 54 (6) ◽  
pp. 956-963 ◽  
Author(s):  
Michael Hartmann ◽  
Monika Schrenk ◽  
Anette Döttinger ◽  
Sarah Nagel ◽  
Johan Roeraade ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Protein Microarray ◽  
Measurement Range ◽  
Multiplex Assay ◽  
Competitive System ◽  
Direct Immunoassay ◽  
Sandwich Configuration ◽  
Readout Channel ◽  
Autoantibody Detection ◽  
Detection And Quantification

Abstract Background: The concurrent detection and quantification of analytes that vary widely in concentration present a principal problem in multiplexed assay systems. Combining competitive and sandwich immunoassays permits coverage of a wide concentration range, and both highly abundant molecules and analytes present in low concentration can be quantified within the same assay. Methods: The use of different fluorescence readout channels allows the parallel use of a competitive system and a sandwich configuration. The 2 generated assay signals are combined and used to calculate the amount of analyte. The measurement range can be adjusted by varying the competitor concentration, and an extension of the assay system’s dynamic range is possible. Results: We implemented the method in a planar protein microarray–based autoimmune assay to detect autoantibodies against 13 autoantigens and to measure the concentration of a highly abundant protein, total human IgG, in one assay. Our results for autoantibody detection and IgG quantification agreed with results obtained with commercially available assays. The use of 2 readout channels in the protein microarray–based system reduced spot-to-spot variation and intraassay variation. Conclusions: By combining a direct immunoassay with a competitive system, analytes present in widely varying concentrations can be quantified within a single multiplex assay. Introducing a second readout channel for analyte quantification is an effective tool for spot-to-spot normalization and helps to lower intraassay variation.

scholarly journals Easy Surface Functionalization and Bioconjugation of Peptides as Capture Agents of a Microfluidic Biosensing Platform for Multiplex Assay in Serum

2021 ◽  
Author(s):  
Concetta Di Natale ◽  
Edmondo Battista ◽  
Vincenzo Lettera ◽  
Narayana Reddy ◽  
Gabriele Pitingolo ◽  
...  
Keyword(s):  
Surface Functionalization ◽  
Multiplex Assay

MULTIPLE ANTIGEN IMMUNIZATION OF INFANTS AGAINST POLIOMYELITIS, DIPHTHERIA, PERTUSSIS, AND TETANUS

PEDIATRICS ◽  
1962 ◽  
Vol 30 (5) ◽  
pp. 720-736
Author(s):  
Clarence D. Barrett ◽  
I. William McLean ◽  
Joseph G. Molner ◽  
Eugene A. Timm ◽  
Charles F. Weiss
Keyword(s):  
Booster Dose ◽  
Active Immunization ◽  
Maternal Antibody ◽  
Control Group ◽  
Tetanus Antitoxin ◽  
The Third ◽  
Multiple Antigen ◽  
Primary Series ◽  
Initial Injection ◽  
Pertussis Antigen

This study was designed to determine the earliest age in infancy at which immunization against poliomyelitis, diphtheria, tetanus, and pertussis can be started using a multiple antigen containing component antigens against all four diseases. Subjects ranged in age from 1 day old through 6 months old at time of initial injection. All were given a series of four injections of 0.5 ml of DPT-polio antigen 4 weeks apart followed by a fifth dose (0.5 ml) of the same material 6 months later. A control group received 0.5 ml of a DPT antigen at monthly intervals for their first four doses, but were given a DPT-polio injection (0.5 ml) for their fifth dose. Although it is evident that there is a progressive response in relation to age of the infant at time of initial inoculation, in respect to poliomyelitis and pertussis immunization, it was apparent that the capacity of the 3-month-old infant to respond to active immunization closely approximates that of the 6-month-old. Ninety per cent showed definite evidence of an immune response to all three poliovirus types despite extremely high levels of preprimary maternal antibody in the majority of 3-month-old infants under study. Pertussis antibody response, as measured by agglutinin titers, was as good in the 3-month-old as in the 6-month-old infants. The response in the 2-month-old infants was relatively poor at the postprimary stage but was equivalent to that of the older infants at the postbooster interval. There was no indication that response to pertussis immunization was impaired by the inclusion of pertussis antigen in the quadrivalent antigen under study. Diphtheria and tetanus antitoxin titers were excellent regardless of age at initial inoculation. The results indicate that four doses of DPT-polio combined antigen given at monthly intervals will overcome the interference of high levels of maternal antibody in respect to poliomyelitis immunization and that the primary series of injections may be started as early as the third month of life. It is important, however, that this primary series of inoculations be followed by a booster dose of the same antigen preparation in about 6 months in order to reinforce the basic immunity.

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