Adventitious rooting in stem and rhizome cuttings of Tuscan (Italy) Vaccinium myrtillus L. under different environmental conditions

2020 ◽  
pp. 1-19
Author(s):  
Stefania Nin ◽  
William Antonio Petrucci ◽  
Adelaide Turchi ◽  
Edgardo Giordani

BACKGROUND: There is no mention in the literature of vegetative propagation of Vaccinium myrtillus. OBJECTIVE: The aim of the study was to evaluate the rooting potential of bilberry rhizome and stem cuttings. METHODS: Rhizome and stem cuttings collected in early Autumn were dipped in different IBA solutions (0 – 2000 – 4000 mgL–1) and rooted in non-sterilized V. myrtillus soil and expanded perlite under controlled growth chamber environment or under mist with basal heating in a greenhouse for 12 weeks. RESULTS: Rhizome cuttings showed high rooting potential in plant growth regulator (PGR) free substrates. Conversely, stem cuttings took advantage from IBA treatments; this positive effect on rooting percentage was synergistically increased when rooted in agriperlite. A higher rooting capacity was achieved when rhizome and stem cuttings were placed in agriperlite, however number of developed roots and shoots were much higher when V. myrtillus turf was used as substrate. Mist and bottom heating did not improve rooting significantly. CONCLUSIONS: V. myrtillus can be successfully propagated both by rhizome and stem cuttings. With an overall rooting success of 56–68% and with a reasonably good growth rate, production of planting material from stem cuttings of V. myrtillus offers opportunities for a cheaper, practically feasible and technically less demanding alternative means of propagation.

2018 ◽  
Vol 12 (2) ◽  
pp. 491-499
Author(s):  
Aurea Portes Ferriani ◽  
Cícero Deschamps ◽  
Wanderlei Do Amaral ◽  
Luiz Everson da Silva

Native Piper species present significant repellent, antimicrobial, inseticidal, anti-tumor and anti-protozoal biological activities. Studies on new species can discover unpublished potentialities and vegetative propagation for the development of cultivation protocols and reduce the natural extraction. The objective of this study was to evaluate the rooting of P. arboreum, P. cernuum and P. diospyrifolium stem cuttings with different treatments containing doses of indole butyric acid (0, 500; 1,000; 1,500; 2,000 and 3,000 mg L-1). Branches were collected in the “Bom Jesus Biological Reserve”, Parana state, Brazil. The cuttings, with a length of 12 cm and average diameter of 6 mm, were placed in 53 cm3 plastic tubes with the commercial substrate Tropstrato HP® and intermittent misting. After 90 days, the survival, mortality, cuttings with new shoots, number and length of the three main roots were evaluated. The percentages of rooting reached an average of 67.5% for P. arboreum, 51.6% for P. cernuum and 50.4% for P. diospyrifolium. A positive effect in the treatments containing the plant regulator was observed for rooting development, percentage of cuttings with shoots and number of roots per propagule, but there were variations in the responses of each species. Leaf retention was an important factor for the adventitious rooting formation in all evaluated species.


Author(s):  
G. A. Pavlenkova ◽  
O. Yu. Emelyanova

Sweet mock orange f. golden dwarf (Philadelphus coronarius L. f. aureus nanus) is a promising ornamental shrub in green building within Central Russia. In the Orel Region, this species has a limited occurrence due to the lack of quality planting material, which warrants research into rhizogenesis of P. coronarius f. aureus nanus stem cuttings during soſtwood graſting. Trials were conducted during 2017-2019 in triplicate, 20 cuttings each, adhering to the common woody plant soſtwood graſting propagation technique. Two yearling types were used, side shoots and innovations. Experimental setups: 1 — control (water); 2 — aqueous indolebutyric acid (IBA) 50 mg/L at 18-h exposure; 3 — bottom cutting powdering with 0.5 g/kg active coal-IBA mixture; 4 — bottom cutting treatment with 50 mg/L IBA at 18-h exposure followed by powdering with 0.5 g/kg active coal-IBA mixture. Setup 2 (IBA 50 mg/L) rendered the highest positive effect on the rooting and reproduction of side shoot and innovation-derived stem cuttings. Te best cutting root system biometry (number and length of 1st order roots) was obtained in setup 2 with the innovation cuttings (10.50 roots of 4.53 cm) compared to the side-shoot ones (9.81 roots of 3.68 cm).


2018 ◽  
Vol 21 (02) ◽  
pp. 64-67
Author(s):  
Mistratova A N ◽  
Bopp L V

The biological effects of sapropel in the technology of growing sea- buckthorn seedlings are studied. It is established that the rooting of green cuttings of the studied varieties on the sapropelic substrate is higher compared to the classic version – peat + sand by 2,0 – 31,3%. It is revealed that the composition of the substrate, the use of the root formation stimulator and varietal characteristics of culture influence the further development of plants grown by the non-transplant method at the place of green cuttings rooting. The most positive effect on the quality of the sea-buckthorn planting material is obtained on the substrate of peat + sand. It is recommended to use the sapropel-based substrate for rooting of the sea-buckthorn stem cuttings in the production conditions, and to apply the transplant method for seedlings further growing.


2021 ◽  
Vol 354 ◽  
pp. 129517
Author(s):  
Priyanka Trivedi ◽  
Nga Nguyen ◽  
Linards Klavins ◽  
Jorens Kviesis ◽  
Esa Heinonen ◽  
...  

1995 ◽  
Vol 73 (3) ◽  
pp. 465-473 ◽  
Author(s):  
Anne Tolvanen

Ramet morphology in the deciduous Vaccinium myrtillus L. and the evergreen Vaccinium vitis-idaea L. was compared between the forest and open, clear-felled habitats. Growth habits were measured in terms of branching, shoot mass, bud type, branching angle, and vertical elevation of ramets. New ramets of both species were produced from buds on the below-ground stem. Branching occurred from buds on 1-year-old shoots in young ramets. Aging and flowering induced rejuvenation, i.e., production of new shoots from buds on older shoot generations within the ramets. Sympodial V. myrtillus ramets were more branched than the predominantly monopodial V. vitis-idaea ramets. In ramets of both species, vegetative shoots grew primarily from distal buds in the forest, whereas a greater number of lower buds were activated in the ramets in the open habitat. Vaccinium myrtillus ramets grew more horizontally and had wider branching angles to increase the intercepted radiation in the forest, whereas compact, vertically growing ramets prevailed in the open habitat, where light was not limited. No difference in branch orientation was observed between habitats in V. vitis-idaea. Production of flowers was greater in the open habitat than in the forest in both species. The observed differences in growth habits between forest and open habitat were assumed to indicate high morphological plasticity in both species, allowing the plants to respond rapidly to changed environmental conditions. Key words: architecture, branching, growth habit, shrub, Vaccinium.


2013 ◽  
Vol 150 (3) ◽  
pp. 446-462 ◽  
Author(s):  
María Ángeles Agulló-Antón ◽  
Almudena Ferrández-Ayela ◽  
Nieves Fernández-García ◽  
Carlos Nicolás ◽  
Alfonso Albacete ◽  
...  

2019 ◽  
Author(s):  
Changzheng Xu ◽  
Yuanxun Tao ◽  
Xiaokang Fu ◽  
Li Guo ◽  
Haitao Xing ◽  
...  

AbstractAdventitious root (AR) formation at the base of stem cuttings determines the efficiency of clonal propagation for woody plants. Many endogenous and environmental factors influence AR formation. However, our knowledge about the regulation of AR development by mitochondrial metabolism in plants is very limited. Here we identified Populus-specific miR476a as a novel regulator of wound-induced adventitious rooting via orchestrating mitochondrial homeostasis in poplar. MiR476a exhibited inducible expression during AR formation and directly targets several Restorer of Fertility like (RFL) genes encoding mitochondrion-localized pentatricopeptide repeat proteins. Genetic modification of miR476-RFL expression revealed the miR476/RFL-mediated dynamic regulation of mitochondrial homeostasis on AR formation in transgenic poplar. Furthermore, mitochondrial perturbation via exogenous chemical inhibitor validated that the miR476a/RFL-directed AR formation depended on mitochondrial regulation though modulating the auxin pathway. Our results established a miRNA-directed mitochondrion-auxin signaling cascade required for AR development, providing novel insights into the understanding of mitochondrial regulation on plant developmental plasticity.


1991 ◽  
Vol 9 (2) ◽  
pp. 83-85
Author(s):  
William H. Rein ◽  
Robert D. Wright ◽  
Dale D. Wolf

Abstract Containerized stock plants of Ilex crenata Thunb. ‘Rotundifolia’ were liquid-fertilized at least three times per week with nitrogen (N) at five rates (25, 50, 100,200, and 300 mg N/l) and in two forms (100% NH4NO3 or 50% NH4NO3 + 50% urea) in a factorial treatment design. Phosphorus and potassium were included in a constant ratio with N (100 N:15 P:50 K). Leaf and stem percent N levels were suboptimal at the lowest fertilizer rate and excessive at the highest fertilizer rate. Percent rooting of stem cuttings decreased linearly with fertilizer rate. With increasing fertilizer rates, total nonstructural carbohydrate (TNC) levels increased in leaves and remained constant in stems. Stem cutting percent moisture increased and was highly correlated with fertilizer rate. The form of N applied made no statistical difference in these trends. The decrease in percent rooting with increasing fertilizer rates was attributed to increases in shoot growth activity and decreased tissue maturation.


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